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Effects of Caffeine on Bone Mineral Density and Bone Mineral Content in Ovariectomized Rats  

Choi, Mi-Ja (Department of Food and Nutrition, Keimyung University)
Lee, Joo-Young (Department of Food and Nutrition, Keimyung University)
Publication Information
Journal of Nutrition and Health / v.41, no.3, 2008 , pp. 216-223 More about this Journal
Abstract
The purpose of this study was to examine the effects of dietary caffeine supplementation on bone mineral density and bone mineral content in ovariectomized rats. Twenty eight female Sprague-Dawley rats (body weight $210\;{\pm}\;5\;g$) were divided into two groups, ovariectomy (OVX) and Sham groups, which were each randomly divided into two subgroups that were fed control and control supplemented with caffeine diets (caffeine 0.03% diets). All rats were fed on experimental diet and deionized water ad libitum for 6 weeks. Bone mineral density (BMD) and bone mineral content (BMC) were measured using PIXImus (GE Lunar Co, Wisconsin) in spine and femur. Serum alkaline phosphatase activity (ALP) and osteocalcin and urinary DPD crosslinks value were measured as markers of bone formation and resorption. The results of this study indicate that body weight gain and food intake were higher in OVX groups than in Sham groups regardless of diets. There were no differences weight gain between the control and caffeine groups in both OVX and Sham groups. Within the OVX groups, serum Ca concentration was lower in rats fed caffeine than in rats fed the control diet. Serum ALP, osteocalcin, urinary Ca, and phosphate were not different in each group. Spine BMD, spine BMD/weight, and spine BMC/weight, femur BMD/weight and femur BMC/weight of ovariectomy groups were significantly lower than Sham groups. Within the OVX group, there were no differences in spine BMD and BMC and femur BMD and BMC. These results indicate that no significant differences in spine and femur BMD were found due to 0.03% caffeine intakes in diet in OVX rats for 6 weeks. No negative effect of caffeine in 0.03% diet on bone mineral density were found in the present study. Further investigation of the relation between caffeine and bone mineral density are warranted. (KoreanJNutr2008; 41(3): 2l6~223)
Keywords
caffeine; BMD; BMC; ovariectomized rats;
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1 Yoon MH, Lee MJ, Hwang SI, Moon SK, Kim JK, Jeong LH, Yim JR. A evaluation of caffeine contents in commercial foods. J Fd Hyg Safety 2001; 16(4): 295-299
2 Harris SS, Dawson-Hughes B. Caffeine and bone loss in healthy postmenopausal women. Am J Clin Nutr 1994; 60: 573-578   DOI
3 Williams JP, Jordan SE, Barnes S, Blair HC. Tyrosine Kinase inhibitor effects on avian osteoclastic acid transport. Am J Clin Nutr 1998(6s); 1369-1374
4 Chung LW, Gleave ME, Hsi JT, Hong SJ, Zhau HE. Reciprocal mesenchymal epithelial interaction affecting prostate tumor growth and hormonal responsiveness. Cancer Survey 1991; 11: 91-121
5 Liu G, Peacock M. Age-related changes in serum undercarboxylated osteocalcin and its relationships with bone density, bone quality, and hip fracture. Calci Tissue Int 1998; 62: 286-289   DOI   ScienceOn
6 Glajchen N. The effect of chronic caffeine administration on serum makers of bone metabolism and bone histomorphometry in the rat. Calcif Tissue Int 1988; 43: 277-280   DOI   ScienceOn
7 Choi MJ, JO HJ. Effect of soy protein and isoflabones on bone mineral dencity and bone mineral content in growing female rats. Korean J Nutr 2003; 36: 359-367
8 Choi MJ, Kang YJ. Effect of isoflabones on bone mineral dencity and bone mineral content in ovariectomized rats. Korean J Nutr 2006; 39: 236-243   과학기술학회마을
9 Choi MK, Lee YS, Sung CJ. Effects of caffeine intake on calcium utilization in rats of different age and sex. Korean J Nutr 1997; 30(8): 911-919
10 Lee HW. A study on caffeine containing foods and the effect of caffeine in humans. Kor J Culinary Res 2000; 6(3): 343-355
11 Choi MJ, Jung JW. Effect of soybean protein on BMD and BMC in ovariectomized rats. Korean J Nutr 2005; 38(4): 279-288   과학기술학회마을
12 Cheung WT, Lee CM, Ng TB. Potentiation of the antilipolytic effect of 2-chloroadenosine after chronic caffeine treatment. Pharmacol 1988; 36: 331-339   DOI   ScienceOn
13 Sakamoto W. Effect of coffee consumption on bone metabolism. Bone 2001; 28(3): 332-336   DOI   ScienceOn
14 Kim MS, Beneficial effect of soy isoflavone on bone loss and hyperlopidemia in ovariectomized rat. Dissertation of Ph. D. Seoul: Seoul National University; 1999
15 Jang HR. The effect of soy isoflabone supplementation on the factors relating to vascular disease in ovariectomized and hyperlipidemic rats. MS Thesis. Seoul: Seoul National University; 2002
16 Lee HJ, Lee HO. A study on the bone mineral density and related factors in korean postmenopausal women. Korean J Nutr 1999; 32(2): 197-203
17 Ohta M, Ide K, Cheuk G, Yazdani M, Nakamoto T, Thomas KA. A caffeine diet can alter the mechanical properties of bones of young ovariectomised rats. Ann Nutr Metab 2002; 46: 108-113   DOI   ScienceOn
18 Lloyd T, Rollings N, Douglas FE, Keselhorst, VM Chinchilli. Dietary caffeine intake and bone status of postmenopausal women. Am J Clin Nutr 1997; 65: 1826-1830   DOI
19 Ohta M, Check G, Ide K, Cheuk S, Wink CS, Falster AU, Simmons WB, Nakamoto T. Effects of caffeine on the bones of aged, ovariectomized rats. Ann Nutr Metab 1999; 43: 52-59   DOI   ScienceOn
20 Nakamoto T, Shaye R. Effects of caffeine on growth of mandible and long bone in protein-energy malnourished newborn rats. Proc Soc Exp Biol Med 1984; 177: 56-61
21 Zapata CLV, Donangelo CM, Woodhouse LR, Abrams SA, Spencer EM, King JC, et al. Calcium homeostasis during pragnancy and lactation in Brazilizn women with low calcium intakes: a longitudinal study. Am J Clin Nutr 2004; 80: 417-422   DOI
22 Korea National Statistical Office, Life Expectancy, Seoul; 2005
23 Bukowiecki LJ, Lupien J, Folla N, Jahjah L. Effect of sucrose, caffeine, and cola beverages on obesity, cold resistance, and adipose tissue cellularity. Am J Physiol 1983; 244: R500-R507
24 Wink Cs, Rossowska MJ, Nakamoto T. Effects of caffeine on bone cells and bone development in fast-growing rats. Pub Med 1996; 246(1): 30-39
25 Mituka BM, Rawnsley HN. Clinical biochemical and hemogtologycal reference value in normal experimental animals and normal humans. 2nd edition, Masson, New York. 1987; p.160
26 Lee YS. Micronutrients and osteoporosis. Korean J Osteoprosis 2005; 3: 206-230
27 Hernandez-Avila M, Colditz GA, Stamter MJ, Rosner B, SpeiZer FE, Willett WC: Caffeine, moderate alcohol intake, and the risk of the hip and forearm in middle-aged women. Am J Clin Nutr 1991; 54: 157-163   DOI
28 Lee JS, Hong HO, Yu CH. A study on the effect of caffeine intake on calcium and phosphorus metabolism in ovariectomized rats. Korean J Nutr 1996; 29(9): 950-957
29 Massey KL, Whiting SJ. Caffeine, urinary calcium, calcium metabolism and bone. J Nutr 1993; 123(9): 1161-1164
30 Ohta M, Cheuk G, Thomos KA, Kamagata-Kiyouraa Y, Winkc CS, Yazdania M, Falsterd AU, Simmonsd WB, Nakamotoa T. Effects of caffeine on bones of aged, ovariectomized rats. Ann Nutr Metab 1999; 43: 52-59   DOI   ScienceOn
31 Greger JL, Emery SM. Mineral metabolism and bone strength of rats fed coffee and decaffeinated coffee. J Agric Food Chem 1987; 35: 551-556   DOI
32 Kim WY, Choi HK, Lee HS. The effects of dietary calcium levels on calcium and skelectal metabolism in ovariectomised rats of different ages. Korean J Nutr 1998; 31(4): 55-62
33 Gallagher SK. Biochemiccal makers of bone metabolism as they relate to osteoporosis. Medical Lab Observer 1997; 29: 50-56
34 Wronski TJ, Cinton M, Doherty AL, Donn LM. Estrgen treatment prevents osteopenia and depresses bone turnover in ovariectomized rats. Endocrinol 1988; 123: 681-688   DOI   ScienceOn
35 Ried IR, Ibbertson HK. Calcium supplements in prevention of steroid- induced osteoporosis. Am J Clin Nutr 1986; 44: 287-290   DOI