• 한국균학회지
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• 제32권2호
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• pp.134-137
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• 2004

항암 능력이 높다고 알려진 진흙버섯속(Phellinus)은 단 다년생인 백색부후균이며 여기에 속하는 10개 균주의 최적 배양조건을 조사하였다. 그 결과 진흙버섯속 균사체 배양에 적절한 영양원으로는 $25^{\circ}C$ 배양상에서 PDA 배지였으며, 최적 pH는 $6.0{\sim}8.0$로 조사되었고 pH 4.0에서는 모든 균주가 느린 생장속도를 나타냈다. 배양온도는 $25{\sim}30^{\circ}C$에서 대부분의 균주가 양호한 생장을 보여주었다. Phellinus biscuspidatus는 실험 균주 중 가장 빠른 생장속도를 나타냈으며 pH 5.0에서 최적의 성장속도를 보여주었으나 pH $6{\sim}8$에서도 양호한 생장을 한 것과 균사밀도를 고려했을 때 최적pH는 $6.0{\sim}7.0$이라 할 수 있겠다. P. biscuspidatus, P. johnsonianus와 P. lloydii는 $30^{\circ}C$와 $35^{\circ}C$ 사이에서 균사생장이 활발한 고온성 균임을 알 수 있다.

• Wind and Structures
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• 제23권4호
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• pp.367-383
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• 2016

The influence mechanism of mean value components, noted as $P_0$, on POD applications for complete random fields $P_C(t)$ and fluctuating random fields $P_F(t)$ are illustrated mathematically. The critical philosophy of the illustration is introduction of a new matrix, defined as the correlation function matrix of $P_0$, which connect the correlation function matrix of $P_C(t)$ and $P_F(t)$, and their POD results. Then, POD analyses for several different wind pressure fields were presented comparatively as validation. It's inevitable mathematically that the first eigenmode of $P_C(t)$ resembles the distribution of $P_0$ and the first eigenvalue of $P_C(t)$ is close to the energy of $P_0$, due to similarity of the correlation function matrixs of $P_C(t)$ and $P_0$. However, the viewpoint is not rigorous mathematically that the first mode represents the mean pressure and the following modes represent the fluctuating pressure when $P_C(t)$ are employed in POD application. When $P_C(t)$ are employed, POD results of all modes would be distorted by the mean value components, and it's impossible to identify $P_0$ and $P_F(t)$ separately. Consequently, characteristics of the fluctuating component, which is always the primary concern in wind pressure field analysis, can only be precisely identified with $P_0$ excluded in POD.

• 대한수학회논문집
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• 제37권4호
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• pp.1055-1072
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• 2022

Our aim is to establish certain image formulas of the (p, 𝜈)-extended Gauss' hypergeometric function F_p,𝜈(a, b; c; z) by using Saigo's hypergeometric fractional calculus (integral and differential) operators. Corresponding assertions for the classical Riemann-Liouville(R-L) and Erdélyi-Kober(E-K) fractional integral and differential operators are deduced. All the results are represented in terms of the Hadamard product of the (p, 𝜈)-extended Gauss's hypergeometric function F_p,𝜈(a, b; c; z) and Fox-Wright function _rΨ_s(z). We also established Jacobi and its particular assertions for the Gegenbauer and Legendre transforms of the (p, 𝜈)-extended Gauss' hypergeometric function F_p,𝜈(a, b; c; z).

• 한국식품위생안전성학회지
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• 제12권3호
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• pp.200-204
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• 1997

Effects of benzoate (0~0.6 g/$\ell$ ) and sorbate (0~2.0 g/$\ell$) on the growth of Escherichia coli O157:H7 in tryptic soy broth at various pH levels (4~8) and temperatures (4$^{\circ}C$, 37$^{\circ}C$) were investigated. Benzoate and sorbate were inhibited the growth of E. coli O157:H7 up to 12 hours cultivation at 4$^{\circ}C$, and 2.0 g/$\ell$ sorbate was only inhibited during 48 hours cultivation at 37$^{\circ}C$. Among the pH levels tested, pH 4 showed significant inhibitory effect against the E. coli O157:H7 on 4$^{\circ}C$ and at 37$^{\circ}C$, respectively. When used in combination 0.2 g/$\ell$ benzoate and sorbate were completely inhibited the growth of E. coli O157:H7 on pH 4 and at 37$^{\circ}C$. While on pH 5 at 4$^{\circ}C$, all of the concentration tested did not exert any inhibitory effect. The combined effects were retarded more than single treatment of E. coli O157:H7.

• IMMUNE NETWORK
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• 제1권3호
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• pp.221-229
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• 2001

Background: Inactivation of tumor suppressor genes is believed to be important in the development of many human malignancies. Recently, several lines of evidence have indicated that the wild type p53 gene located at 17p13.3, may function as a tumor suppressor gene and that a mutant p53 gene could promote transformation by inactivating normal p53 function in a dominant negative fashion. These broad spectrum of p53 mutation in human cancers provide that mutant p53 and their protein may be potential targets of tumor diagnostic and therapeutic interventions. Method: Colony formation was performed to investigate growth suppressional ability. p53 expression pattern was examined by western blot and p53-mediated transactivation ability was assessed by CAT activity. SNU C2A cells were observed in apoptotic aspects induced by etoposide and $H_2O_2$ treatment, detecting sensitivity on agent, DNA fragmentation through agarose gel, chromatin condensation by fluorescence microscope, and cell cycle distribution by FACS. Result: 1) p53 mutant his179arg ($histidine{\rightarrow}arginine$) detected in SNU C2A cells lost transcriptional activity and growth suppression ability, showing dominant negative effect on its wild type p53. 2) Etoposide-treated SNU C2A cells induced apoptosis, exhibiting dramatic reduction of cell growth, DNA fragmentation, nuclear condensation formation of apoptotic body and increment of sub-G1 cell fraction. 3) Etoposide and $H_2O_2$-treated SNU C2A cells have no high increase of p53 expression and overexpressed p53 protein changed localization, from cytoplasm to nucleus. Also, p53-mediated transcriptional activity was increased by agents-treatment. Conclusion: SNU C2A cells coexpress wild-type and mutant p53 protein induced apoptosis in the condition on DNA damage, through localizational shift from cytoplasm to nucleus of p53 protein rather than the induction of p53 protein. SNU C2A cells derived mutant p53 his179arg abrogated both the growth supression ability and transactivational activity, showing inhibition effect on transcriptional activity of wild type p53, but did not repress the activity of wild type p53 in SNU C2A cells owing to dominant activity of wild type. These cell condition may provide new gene therapeutic implications leading effective antiproliferation of cell when mutant and wild-type p53 protein were co-expressed in cell.

• 미생물학회지
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• 제47권1호
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• pp.92-96
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• 2011

Rhodococcus sp. strain DK17은 3개의 linear catabolic megaplasmid (380 kb pDK1, 330 kb pDK2, 750 kb pDK3)를 보유하고 있다. Alkylbenzene 분해 유전자군(akbABCDEF)은 pDK2에만 위치하는 반면 phthalate 분해 유전자군의 경우에는 거의 동일한 copy가 pDK2 (ophA'B'C'R')와 pDK3 (ophABCR)에 각각 위치한다. DK17은 최적 성장온도인 $30^{\circ}C$에서 $37^{\circ}C$로 배양온도를 상승시키면 성장이 멈추지만 $30^{\circ}C$로 환원 즉시 다시 성장을 시작하였다. 이는 $37^{\circ}C$라는 온도 조건이 DK17에게 치명적이지는 않지만 스트레스로 작용함을 암시하는 것이다. 또한 $37^{\circ}C$에서 열충격을 받은 일부 세포는 o-xylene (alkylbenzene의 모델 화합물)을 분해 능력을 상실하였다. 열충격 후 200개의 집락을 무작위로 선택하여 pDK2- 또는 pDK3-specific 프라이머를 이용한 PCR과 pulsed-field gel electrophoresis를 통해 megaplasmid의 변화 양상을 비교 분석한 결과, 29개 돌연변이체에서 야생형의 것과 다른 5가지 megaplasmid 유형[pDK2 소실과 함께 새로운 약 700 kb megaplasmid 출현(10개), pDK2만 소실(9개), pDK3 소실과 함께 새로운 약 400 kb megaplasmid 출현(8개), pDK3만 소실(1개), 그리고 pDK2와 pDK3 소실과 함께 각각 약 400 kb와 700 kb 크기의 새로운 megaplasmid 출현(1개)]이 관찰되었다. 이상의 연구 결과는 성장 온도 변화가 세균 유전체의 물리적 변화를 직접 유도할 수 있음을 입증하는 것으로 온도 변화를 비롯한 서식지 환경 변화가 세균 진화에 상당한 영향력을 미칠 수 있음을 보여준다.

• 미생물학회지
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• 제5권1호
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• pp.20-23
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• 1967

I studied about temperature and pH concentration having influence upon chlorophyll decrease on the Glucose culture of Chlorella variegata Beijerinck(211/10a). I cultured under $25^{\circ}C$(standard), $15^{\circ}C$ and $35^{\circ}C$ and compared with each other. The culture of $15^{\circ}C$ didn't have any large difference with the standard culture($25^{\circ}C$) but the culture of $35^{\circ}C$ had a large amount of chlorophyll decrease without carbohydrate accumulation, stimulation of cell division and nitrogen-deficiency. Chlorella variegata had optimum pH 6.5-7 and was a little weak in all phenomenon under pH 8 rather than under pH 6.5-7. Under pH 5 they had deep chlorophyll decrease without phephytin.

• 한국분말재료학회지
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• 제4권1호
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• pp.26-41
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• 1997

The powder metallurgy (P/M) processed 2009 and 2124 Al composites reinforced with SiC particulates were studied by focusing on the effect of consolidation temperature on the microstructural and mechanical Properties. The mechanical properties such as tensile properties and microhardness of the second phases were analysed in relation to the microstructures observed by a SEM and an optical microscope. The in situ fracture process study using SEM showed that the grain refinement and the removal of manganese-containing particles often observed in the 2124 Al-${SiC}_{p}$ composites were important for the improvement of the mechanical properties. This study offers an optimum consolidation temperature for the control of the manganese-containing particles in the 2124 Al-${SiC}_{p}$ composites that yields mechanical properties higher than those of the 2009 Al-${SiC}_{p}$ composites.

• Elastomers and Composites
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• 제7권1호
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• pp.53-58
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• 1972

Sublimatographic study on the method of test for sulfur in the petroleum and rubber products was as following results: (1) The relation between the heating temperature$(t_h)$ and the vacuum condensing point(V.C.P.) of sulfur at different degree of vacuum appeared as Fig.4. The results were in good agreement with those expected from the $t_h-V.C.P$ curves of sublimatographic separation of sulfur. (2) The relation between the degree of vacuum and the V.C.P. at different heating temperature of sulfur appeared as follows; $$tv.c.p.=\alpha+{\beta}logP_{va}$$ (3) The V.C.P of sulfur could be physical property comparing with melting point and boiling point. (4) The relation between the V.C.P. and the quantity of sublimation of sulfur becomes quantitative as Fig.7.

• 대한화학회지
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• 제14권4호
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• pp.321-326
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• 1970

The relation between the heating temperature and the V.C.P(Vacuum Condensing Point) at different degree of vacuum through the sublimatographic separation was studied where by ; (1) Anthracene and Anthraquinone, ${\alpha}$-Naphtol and ${\beta}$-Naphthol, o-Aminobenzoicacid and p-Aminobeenzoicacid were easily separated from each of its mixtures as shown in Figure9, 10 and 11, while tailings appeared appreciably. The results were in good agreement with those expected from the $t_k$-V.C.P curves in Figures 3,4,5,6,7 and 8. (2) The relation between the degree of vacuum and the V.C.P. of ${\alpha}$-Naphthol and Anthracene at different heating temperatures appeared as follows and are shown in Figures 12 and 13.