• Journal of Ecology and Environment
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• 제30권4호
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• pp.331-340
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• 2007

We examined the evolutionary and ecological processes shaping current geographical distributions of a Korean wood-eating cockroach species, Cryptocercus kyebangensis. Our research aims were to understand evolutionary pattern of DNA sequences, to construct genetic network of Cryptocercus kyebangensis local populations and to understand evolutionary and ecological processes shaping their current geographical distribution patterns via DNA sequence information and genetic networks, using sequence data of two genes (ITS-2 and AT region) from local populations of C. kyebangensis. The results suggest that the ITS-2 and AT region are appropriate molecular markers for elucidating C. kyebangensis geographic patterns at the population level. The MSN-A based on the ITS-2 showed two possible routes, the Hwaak-san and Myeongji-san route and the Seorak-san and Gyebang-san route, for migration of ancestral C. kyebangensis into South Korea. The MSNs (MSN-A and -B) elucidate migration routes well within South Korea, especially the route of Group I and Group II.

• Journal of Plant Biology
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• 제37권2호
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• pp.167-173
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• 1994

Poly(A)+ RNA was selected from Canavalia lineata root nodule RNA through oligo(dT) cellulose column and used for construction of a cDNA library using λgt10-EcoRI arms. The size of the library was 7.2$\times$105 pfu/mL. A full length leghemoglobin (Lb) cDNA clone, pCILb1(687 bp) isolated with soybean Lb probe, contained one open reading frame (ORF) of 447 bp with 54 bp plus 186 bp at 5' and 3' untranslated region, respectively. A consensus sequence of plant translation start region (AAAATGGG) was found at 5' untranslated region, and two polyadenylation-related sequence (AATAAA, AATAAG) and a conserved motif between them (gACTTGTT) were found upstream of poly(A)+ tail consisted of 13 (A)s at 3' untranslated region. The ORF encoded a polypeptide consisted of 149 amino acids with a molecular weight of 16.2 kD. Deduced amino acid sequences showed high degree of homology values with those of other Lbs ranging from 66% (Casuarina glauca) to 85% (Glycine max).

• 생명과학회지
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• 제8권1호
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• pp.102-108
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• 1998

Gltcosylphosphatidylinositol (GPI)에 의해 고정된 단백질의 초기 형태는 골지체에서의 직접적인 processing을 수행하기 위한 아미노와 카르 복시 말단의 hydrophobic signal sequence를 소유하고 있다. 앞서, mouse 임파구로부터 NAD;arginine ADP-ribosyltransferase (Yac-1)가 클로닝되었으며 Yac-1 transferase의 아미노산 배열을 추정해 본 결과, hydrophobic 아미노와 카르복시 말단을 포함하고 있었으며 이는 GPI-anchroed 단백질들의 알려진 signal sequence와 일치하였다. 미 transferase는 야생형의 cDNA로 transfection된 NMU (rat mammary adenocarcinoma) cell의 표면에 존재하였으며 phosphoatidylinosotol-specific phospholipase C에 의해 방출되어졌다. 카르복시 말단의 hydrophobic sequence가 없는 돌연변이체는 수용성이며 분비성인 transferase를 생산하였다. 이러한 사실은 카르복시 말단의 sequence가 없는 돌연변이체는 수용성이며 분비성인 transferase를 생산하였다. 이러한 사실은 카르복시 말단의 sequence가 GPI의 부착에 중요함을 나타내준다.

• 한국미생물학회:학술대회논문집
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• 한국미생물학회 2000년도 추계학술발표대회
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• pp.15-23
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• 2000

The subcellular localization of the SopB protein, which is encoded by the Escherichia coli F plasmid and is involved in the partition of the single-copy plasmid, was directly visualized through the expression of the protein fused to the jellyfish green fluorescent protein (GFP). The fusion protein was found to localize to positions close but not at the poles of exponentially growing cells. Examination of derivatives of the fusion protein lacking various regions of SopB suggests that the signal for the cellular localization of SopB resides in a region close to its N terminus. Overexpression of SopB led to silencing of genes linked to, but well-separated from, a cluster of SopB-binding sites termed sopC. In this SopB-mediated repression of sopC-linked genes, all but the N-terminal 82 amino acids of SopB can be replaced by the DNA-binding domain of a sequence-specific DNA -binding protein, provided that the sopC locus is also replaced by the recognition sequence of the DNA-binding domain. These results suggest a mechanism of gene silencing: patches of closely packed DNA-binding protein is localized to specific cellular sites; such a patch can capture a DNA carrying the recognition site of the DNA -binding domain and sequestrate genes adjacent to the recognition site through nonspecific binding of DNA.

• 한국정보통신학회논문지
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• 제10권12호
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• pp.2329-2334
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• 2006

본 논문에서는 기존의 conting 구성 프로그램의 단점인 단편들 간의 결합 실패를 보완하는 알고리즘을 제안하였다. 제안된 방법은 매우 긴 DNA의 염기 서열을 자동 서열 분석기로 한번에 분석 가능한 약 700개의 단편들을 한 주형으로 만들어 PCR 방법으로 클론 3을 생성 후, $600\sim700$개의 길이로 단편화하여 기준 주형과 비교하여 일치율을 계산한다. 이때 Compute Agreement 알고리즘을 이용하여 일치율을 계산하는 시간을 단축시킨다. 계산된 단편 쌍들의 중첩 정도를 기준으로 주형마다 2개의 결합 후보 단편을 추출하여 추출된 각 단편들의 일치율과 각 DNA 염기의 A,G,C,T 소속도 및 각 A,G,C,T 이 전 빈도수를 퍼지 추론 규칙을 이용하여 결합 여부를 판단한다. 본 논문에서는 결정된 최 적의 비교 단편을 결합하고, 더 이상 단편이 없을 때까지 반복하여 서열 결합을 완성한다. 실험을 위해 완성된 단백질 지놈인 'Synechocystis PCC6803'을 각각 1만개, 10만개씩 추출하여 $600{\sim}700$개의 길이를 가진 단편을 생성하였으며, 이 단편을 임 의의 mutation을 유발하여 실험한 결과, FAP 프로그램보다 속도가 줄어들었으며, conting 구성 프로그램의 단점 인 결합 실패가 발생하지 않았다.

• Ocean Science Journal
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• 제40권1호
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• pp.55-59
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• 2005

We cloned the complete cDNA of the ${\beta}-bubulin$ from the soft coral, Scleronephthya gracillimum $(K\ddot{u}kenthal)$ (Alcyonacea, Octocorallia, Anthozoa, Cnidaria), via the random sequencing of a cDNA library and the 5'-rapid amplification of cDNA end (RACE) technique. The full-length cDNA of the S. gracillimum ${\beta}-tubulin$ comprised 1541 bp, not including the poly $A^+$ stretch, also contained a complete open reading frame, which codes for a total of 445 amino acids. The amino acid residues 16402 appeared to be in a state of conservation in a variety of animals. Northern blot analysis clearly demonstrated that the sequence we have obtained is, indeed, the full-length cDNA of the ${\beta}-bubulin$ gene in S. gracillimum.

• International Journal of Industrial Entomology and Biomaterials
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• 제4권1호
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• pp.63-68
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• 2002

A cDNA encoding a putative member of cathepsin B of the thiol pretense superfamily was cloned from a cDNA library of the mulberry longicorn beetle, Apriona germari. Sequence analysis of the cDNA encoding the cathepsin B of A. germari (AgCatB) revealed that the 972 bp cDNA has an open reading frame of 324 amino acid residues. The deduced protein sequence of the AgCatB showed high homology with cathepsin B of the insects, Bombyx mori (47.3% amino acid identity), Helicoverpa armigera (46.6%) and Sarcophaga peregrina (45.6%), and the lowest homology with Aedes aegypti (33.2%). The AgCatB contains six disulfate bonds typical for cysteine pretenses. The three amino acid positions Cys-109, His-267, and Asn-287 which are conserved, active sites characteristic for cathepsin B, were also found. Phylogenetic analysis further confirmed that the AgCatB has a close relationship with that of B. mori, H. armigera and S. peregrina.

• 한국어병학회지
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• 제20권3호
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• pp.299-306
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• 2007

Cysteine-cysteine chemokine receptor 9 (CCR9) homologue cDNA was isolated from olive flounder leukocyte cDNA library. Olive flounder CCR9 homologue consisted of 1709 bp encoding 367amino acid residues. When compared with other known CCR peptide sequences, the most conserved region of the olive flounder CCR9 peptide is the seven transmembranes. A phylogenetic analysis based on the deduced amino acid sequence showed the homologous relationship between the olive flounder CCR9 sequence and that of Mouse CCR9. The olive flounder CCR9 gene was predominantly expressed in the Peripheral blood leukocytes (PBLs), kidney, spleen, and gills.

• International Journal of Industrial Entomology and Biomaterials
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• 제11권2호
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• pp.113-117
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• 2005

A chymotrpsin gene homologue was cloned from the mulberry longicorn beetle, Apriona germari. The A. germari chymotrypsin cDNA contains an ORF of 950 nucleotides capable of encoding a 283 amino acid polypeptide with a predicted molecular mass of 29151 Da and pI of 9.38. The A. germari chymotrypsin has conserved six cysteine residues and active triad formed by His, Asp and Ser. The deduced amino acid sequence of the A. germari chymotrypsin cDNA was closest in structure to the Anthonomus grandis chymotrypsin. Northern blot analysis revealed that A. germari chymotrypsin showed the midgut-specific expression.

• Fisheries and Aquatic Sciences
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• 제6권2호
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• pp.101-104
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• 2003

A cDNA clone for the olive flounder (Paralichthys olivaceus) transferrin receptor (fTfR) was isolated from a leukocytes cDNA library. The fTfR gene consisted of 2,319 bp encoding 773 amino acid residues. The amino acid sequence alignment of the fTfR showed that their size and hydrophobic profile are similar. In addition, the Tyr-Thr-Arg-Phe (YTRF) motif that is the recognition signal for high-efficiency endocytosis, is conserved very well. This motif is important for functional properties of TfR. The deduced amino acid sequence had $42.4-42.9\%$ identities with the previously reported TfRs of vertebrates. The fTfR was expressed in the blood, kidney, spleen, and liver of healthy olive flounder by the Northern blot hybridization.