International Journal of Industrial Entomology and Biomaterials

Korean Society of Sericultural Science (한국잠사학회)
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A Chymotrypsin Gene Homologue from the Mulberry Longicorn Beetle, Apriona germari: cDNA Sequence Characterization and mRNA Expression Pattern

  • Gui Zong Zheng (College of Natural Resources and Life Science, Dong-A University) ;
  • Lee Kwang Sik (College of Natural Resources and Life Science, Dong-A University) ;
  • Yoon Hyung Joo (Department of Agricultural Biology, National Institute of Agricultural Science and Technology) ;
  • Kim Iksoo (Department of Agricultural Biology, National Institute of Agricultural Science and Technology) ;
  • Sohn Hung Dae (College of Natural Resources and Life Science, Dong-A University) ;
  • Jin Byung Rae (College of Natural Resources and Life Science, Dong-A University)
  • Published : 2005.12.01
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Abstract

A chymotrpsin gene homologue was cloned from the mulberry longicorn beetle, Apriona germari. The A. germari chymotrypsin cDNA contains an ORF of 950 nucleotides capable of encoding a 283 amino acid polypeptide with a predicted molecular mass of 29151 Da and pI of 9.38. The A. germari chymotrypsin has conserved six cysteine residues and active triad formed by His, Asp and Ser. The deduced amino acid sequence of the A. germari chymotrypsin cDNA was closest in structure to the Anthonomus grandis chymotrypsin. Northern blot analysis revealed that A. germari chymotrypsin showed the midgut-specific expression.

Keywords

References

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