Nucleotide Sequence of ${\beta}-tubulin$ Gene from the Soft Coral Scleronephthya gracillimum $(K\ddot{u}kenthal)$

  • Yum, Seung-Shic (Southern Coastal Environment Research Division, South Sea Institute, KORDI) ;
  • Woo, Seon-Ock (Southern Coastal Environment Research Division, South Sea Institute, KORDI) ;
  • Chang, Man (Southern Coastal Environment Research Division, South Sea Institute, KORDI) ;
  • Lee, Taek-Kyun (Southern Coastal Environment Research Division, South Sea Institute, KORDI) ;
  • Song, Jun-Im (Department of Biological Science, College of Natural Sciences, Ewha Womans University)
  • Published : 2005.03.31

Abstract

We cloned the complete cDNA of the ${\beta}-bubulin$ from the soft coral, Scleronephthya gracillimum $(K\ddot{u}kenthal)$ (Alcyonacea, Octocorallia, Anthozoa, Cnidaria), via the random sequencing of a cDNA library and the 5'-rapid amplification of cDNA end (RACE) technique. The full-length cDNA of the S. gracillimum ${\beta}-tubulin$ comprised 1541 bp, not including the poly $A^+$ stretch, also contained a complete open reading frame, which codes for a total of 445 amino acids. The amino acid residues 16402 appeared to be in a state of conservation in a variety of animals. Northern blot analysis clearly demonstrated that the sequence we have obtained is, indeed, the full-length cDNA of the ${\beta}-bubulin$ gene in S. gracillimum.

Keywords

References

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