• Molecules and Cells
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• v.37 no.3
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• pp.213-219
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• 2014

MicroRNAs (miRNAs) represent a class of small non-coding regulatory RNAs that play important roles in normal hematopoiesis, including erythropoiesis. Although studies have identified several miRNAs that regulate erythroid commitment and differentiation, we do not understand the mechanism by which the crucial erythroid transcription factors, GATA-1and NF-E2 directly regulate and control differentiation via miRNA pathways. In this study, we identified miR-199b-5p as a key regulator of human erythropoiesis, and its expression was up-regulated during the erythroid differentiation of K562 cells. Furthermore, the increase of miR-199b-5p in erythroid cells occurred in a GATA-1- and NF-E2-dependent manner during erythrocyte maturation. Both GATA-1 and NF-E2 bound upstream of the miR-199b gene locus and activated its transcription. Forced expression of miRNA-199b-5p in K562 cells affected erythroid cell proliferation and maturation. Moreover, we identified c-Kit as a direct target of miR-199b-5p in erythroid cells. Taken together, our results establish a functional link among the erythroid transcription factors GATA-1/NF-E2, miR-199b-5p and c-Kit, and provide new insights into the coupling of transcription and post-transcription regulation in erythroid differentiation.

• Clinical and Experimental Reproductive Medicine
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• v.32 no.3
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• pp.207-216
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• 2005

Objective: To understand the crucial requirement for the normal early folliculogenesis, we evaluated molecular as well as physiological differences during in vitro ovarian culture. Among the important regulators for follicle development, anti-Müllerian hormone (AMH) and FSH Receptor (FSHR) have been known to be expressed in the cuboidal granulosa cells. Meanwhile, it is known that c-kit is germ cell-specific and GDF-9 is also oocyte-specific regulator. To evaluate the functional requirement for the competence of normal follicular development, we investigated the differential mRNA expression of several factors secreted from granulosa cells and oocytes between in vivo and in vitro developed ovaries. Materials and Methods: Ovaries from ICR neonates (the day of birth) were cultured for 4 days (for primordial to primary transition) or 8 days (for secondary follicle formation) in ${\alpha}$-MEM glutamax supplemented with 3 mg/ml BSA without serum or growth factors. The mRNA levels of the several factors were investigated by quantitative real-time PCR analysis. Freshly isolated 0-, 4-, and 8-day-old ovaries were used as control. Results: The mRNA of AMH and FSHR as granulosa cell factors was highly increased according to the ovarian development in both of 4- and 8-day-old control. However, the mRNA expression was not induced in both of 4- and 8-day in vitro cultured ovaries. The mRNA expression of GDF-9 known to regulate follicle growth as an oocyte factor was different between in vivo and in vitro developed ovaries. In addition, the transcript of GDF-9 was expressed in the primordial follicles of mouse ovaries. The mRNA expression of c-kit was not significantly different during the early folliculogenesis in vitro. Conclusion: This is the first report regarding endogenous AMH and FSHR expression during the early folliculogenesis in vitro. In conclusion, it will be very valuable to evaluate cuboidal granulosa cell factors as functional marker(s) for normal early folliculogenesis in vitro.

• Proceedings of the IEEK Conference
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• 2005.11a
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• pp.651-654
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• 2005

In this paper, we designed ECC(Elliptic Curve Cryptographic) Processor with Bus-splitting mothod for embedded SoC. ECC SIP is designed by VHDL RTL modeling, and implemented reusably through the procedure of logic synthesis, simulation and FPGA verification. To communicate with ARM9 core and SIP, we designed SIP bus functional model according to AMBA AHB specification. The design of ECC Processor for platform-based SoC is implemented using the design kit which is composed of many devices such as ARM9 RISC core, memory, UART, interrupt controller, FPGA and so on. We performed software design on the ARM9 core for SIP and peripherals control, memory address mapping and so on.

• Proceedings of the KIEE Conference
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• 2005.10b
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• pp.379-381
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• 2005

Urodynamics describes a collection of tests designed to evaluate lower urinary tract function and can be performed using retrograde filling of the bladder within a room. In this study, we designed and calibrated the potable urodynamics monitoring system using DSP chip (TMS320VC33, Texas InstrumentTM, U.S.) and obtained signals of bladder(Pves) and bladder neck pressure(Pneck) and EMG using calibration kit (DPT9022K0122, MedtronicsTM, U.s,). This system monitor spontaneous urination during daily life and can make patients more comportable.

• Journal of Applied Biological Chemistry
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• v.61 no.1
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• pp.59-67
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• 2018

This study was conducted to isolate the cellulolytic bacteria able to grow on LB- Carboxymethyl cellulose (CMC) agar trypan blue medium from the mixed forest and Larix leptolepis stands. Three bacterial strains with high activity against both CMC and xylan were isolated. Both API kit test and 16S rRNA gene sequence analysis revealed that the three different isolates belong to the gene Bacillus. Therefore, the isolates named as Bacillus sp. EFL1, Bacillus sp. EFL2, and Bacillus sp. EFP3. The optimum growth temperature of Bacillus sp. EFL1, EFL2, and EFP3 were $37^{\circ}C$. The optimum temperature for CMCase and xylanase from Bacillus sp. EFL1 were $50^{\circ}C$. The optimum pH of Bacillus sp. EFL1 xylanase was pH 5.0 but the optimum pH of CMCase from Bacillus sp. EFL1 was pH 6.0. The optimum temperature of CMCase and xylanase from Bacillus sp. EFL2 was $60^{\circ}C$, respectively. The optimum pH of CMCase of Bacillus sp. EFL2 was 5.0, whereas xylanase showed high activity at pH 3.0-9.0. The optimum temperature for CMCase and xylanase of Bacillus sp. EFP3 was $50^{\circ}C$. The optimum pH for CMCase and xylanse was 5.0 and 4.0, respectively. CMCases from Bacillus sp. EFL1, EFL2, and EFP3 were thermally unstable. Although xylanase from Bacillus sp. EFL1 and EFP3 showed to be thermally unstable, xylanase from Bacillus sp. EFL2 showed to be thermally stable. Therefore, Bacillus sp. EFL2 has great potential for animal feed, biofuels, and food industry applications.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2009.10a
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• pp.465-468
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• 2009

A frequency in electrical power system changes by the load fluctuation in utility grid, has an influence on a connected generator, and ultimately brings a big trouble in the power system. Therefore, a quick measurement of system frequency and governor control of power system is a very important factor in the reliability and the economic feasibility. In this study, An improve algorithm that measures the power system frequency quickly and accurately is suggested, simulated by using Matlab and programmed using C code through DSP6713 KIT. This algorithm is tested to the arbitrary voltage waveform input. The results show that the suggested algorithm is effective in the accurate and quick frequency measurements.

• Electronics and Telecommunications Trends
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• v.30 no.2
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• pp.87-94
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• 2015

인터넷 트래픽의 향후 5년간 연평균성장률(CAGR)은 24%(유선 트래픽이 21%, 모바일 트래픽이 68%)로 예상되지만, 인터넷 트래픽을 처리하는 칩셋의 성능 연평균성장률은 14% 정도로 예상되고 있다. 이에 따라, 증가하는 인터넷 트래픽과 이를 처리하는 칩셋의 성능 사이에 격차(Forwarding Gap)가 발생하고 있는 상황이다. 이런 격차를 줄이기 위해 시작된 연구기술이 데이터 플레인 가속화(DPA: Data Plane Acceleration) 기술이다. 본고에서는 데이터 플레인 가속화 기술로 최근 공개 소프트웨어로 발표된 인텔의 DPDK(Data Plane Development Kit)기술과 Linaro의 ODP(Open Data Plane)기술을 중심으로 고속 네트워크 패킷처리를 위한 데이터 플레인 가속화 기술동향을 소개한다.

• Proceedings of the Korean Society of Veterinary Clinics Conference
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• 2008.05a
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• pp.124-124
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• 2008

• Proceedings of the KIEE Conference
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• 1987.07a
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• pp.201-204
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• 1987

To improve the reliability of boiler controller of a power plant, FTCS(Fault Tolerant Control System) is proposed. We studied to implement a Multi-processor scheme for FTCS. This paper presents the total system to experiment the performance of FTCS and the Multi-processor scheme implemented.

• Proceedings of the Korean Information Science Society Conference
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• 2004.10a
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• pp.553-555
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• 2004

SoC 분야에서 온 칩 버스는 전체 시스템의 성능을 결정하는 중요한 요소이다. 이에 따라 최근 ARM 사에서는 고성능 온 칩 버스 구조인 ML(Multi-Layer) AHB 버스를 제안하였다. ML AHB 버스는 저전력 임베디드 시스템에 적합한 버스 구조로써 현재 널리 사용되고 있다. 하지만, 고가이기 때문에 ADK(AMBA$^{TM}$ Design kit) 구매에 대한 부담이 적지 않다. 본 논문은 ML AHB의 버스 구조인 버스 매트릭스 구현 및 ADK에서 제공되지 않는 테스트 환경 즉, Protocol Checker 및 Performance Monitor Module 구현에 관한 것이다.