Clinical and Experimental Reproductive Medicine

  • 제32권3호
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  • Pages.207-216
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  • 2005
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  • 2233-8233(pISSN)
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  • 2233-8241(eISSN)
대한생식의학회 (The Korean Society for Reproductive Medicine)
권호 표지

체외배양 중인 생쥐 난소에서 초기난포 조절인자의 발현

mRNA Expression of the Regulatory Factors for the Early Folliculogenesis in vitro

  • 윤세진 (차병원 여성의학연구소) ;
  • 김기령 (포천중문 의과대학교 생명과학대학원) ;
  • 정형민 (차병원 여성의학연구소) ;
  • 윤태기 (차병원 여성의학연구소) ;
  • 차광렬 (차병원 여성의학연구소) ;
  • 이경아 (차병원 여성의학연구소)
  • Yoon, Se-Jin (Infertility Medical Center, CHA General Hospital) ;
  • Kim, Ki-Ryeong (Graduate School of Life Science and Biotechnology, Pochon CHA University College of Medicine) ;
  • Chung, Hyung-Min (Infertility Medical Center, CHA General Hospital) ;
  • Yoon, Tae-Ki (Infertility Medical Center, CHA General Hospital) ;
  • Cha, Kwang-Yul (Infertility Medical Center, CHA General Hospital) ;
  • Lee, Kyung-Ah (Infertility Medical Center, CHA General Hospital)
  • 발행 : 2005.09.30
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초록

Objective: To understand the crucial requirement for the normal early folliculogenesis, we evaluated molecular as well as physiological differences during in vitro ovarian culture. Among the important regulators for follicle development, anti-Müllerian hormone (AMH) and FSH Receptor (FSHR) have been known to be expressed in the cuboidal granulosa cells. Meanwhile, it is known that c-kit is germ cell-specific and GDF-9 is also oocyte-specific regulator. To evaluate the functional requirement for the competence of normal follicular development, we investigated the differential mRNA expression of several factors secreted from granulosa cells and oocytes between in vivo and in vitro developed ovaries. Materials and Methods: Ovaries from ICR neonates (the day of birth) were cultured for 4 days (for primordial to primary transition) or 8 days (for secondary follicle formation) in ${\alpha}$-MEM glutamax supplemented with 3 mg/ml BSA without serum or growth factors. The mRNA levels of the several factors were investigated by quantitative real-time PCR analysis. Freshly isolated 0-, 4-, and 8-day-old ovaries were used as control. Results: The mRNA of AMH and FSHR as granulosa cell factors was highly increased according to the ovarian development in both of 4- and 8-day-old control. However, the mRNA expression was not induced in both of 4- and 8-day in vitro cultured ovaries. The mRNA expression of GDF-9 known to regulate follicle growth as an oocyte factor was different between in vivo and in vitro developed ovaries. In addition, the transcript of GDF-9 was expressed in the primordial follicles of mouse ovaries. The mRNA expression of c-kit was not significantly different during the early folliculogenesis in vitro. Conclusion: This is the first report regarding endogenous AMH and FSHR expression during the early folliculogenesis in vitro. In conclusion, it will be very valuable to evaluate cuboidal granulosa cell factors as functional marker(s) for normal early folliculogenesis in vitro.

키워드

과제정보

연구 과제 주관 기관 : 한국과학재단

참고문헌

  1. Hirshfiokld AN. Development of follicles in the mammalian ovary. Int Rev Cytol 1991; 124: 43-101 https://doi.org/10.1016/S0074-7696(08)61524-7
  2. Parrott JA, Skinner MK. Kit-ligand/stern cell factor induces primordial follicle development and initiates folliculogenesis. Endocrinology 1999; 140: 4262-71 https://doi.org/10.1210/en.140.9.4262
  3. Yitt UA, McGee EA, Hayashi M, Hsueh AJ. In vivo treatment with GDF-9 stimulates primordial and primary follicle progression and theca cell marker CYP17 in ovaries of immature rats. Endocrinology 2000; 141: 3814-20 https://doi.org/10.1210/en.141.10.3814
  4. Nilsson E, Parrott JA, Skinner MK. Basic fibroblast growth factor induces primordial follicle development and initiates folliculogenesis. Mol Cell Endocrinol 2001; 175: 123-30 https://doi.org/10.1016/S0303-7207(01)00391-4
  5. Dissen GA, Romero C, Hirshfield AN, Ojeda SR. Nerve growth factor is required for early follicular development in the mammalian ovary. Endocrinology 2001; 142: 2078-86 https://doi.org/10.1210/en.142.5.2078
  6. Nilsson EE, Kezele P, Skinner MK. Leukemia inhibitory factor (LIF) promotes the primordial to primary follicle transition in rat ovaries. Mol Cell Endocrinol 2002; 188: 65-73 https://doi.org/10.1016/S0303-7207(01)00746-8
  7. Kezele PR, Nilsson EE, Skinner MK. Insulin but not insulin-like growth factor-1 promotes the primordial to primary follicle transition. Mol Cell Endocrinol 2002; 192: 37-43 https://doi.org/10.1016/S0303-7207(02)00114-4
  8. Lee WS, Yoon SJ, Yoon TK, Cha KY, Lee SH, Shimasaki S, Lee S, Lee KA. Effects of bone morphogenetic protein-7 (BMP-7) on primordial follicular growth in the mouse ovary. Mol Reprod Dev 2004; 69: 159-63 https://doi.org/10.1002/mrd.20163
  9. Durlinger AL, Gruijters MJ, Kramer P, Karels B, Ingraham HA, Nachtigal MW,Uilenbroek JT, Grootegoed JA, Themmen AP. Anti-Mullerian hormone inhibits initiation of primordial follicle growth in the mouse ovary. Endocrinology 2002; 143: 1076-84 https://doi.org/10.1210/en.143.3.1076
  10. Schmidt KL, Kryger-Baggesen N, Byskov AG, Andersen CY. Anti-Mullerianhormone initiates growth of human primordial follicles in vitro. Mol Cell Endocrinol 2005; 234: 87-93 https://doi.org/10.1016/j.mce.2004.12.010
  11. Driancourt MA, Reynaud K, Cortvrindt R, Smitz J. Roles of KIT and KIT LIGAND in ovarian function. Rev Reprod 2000; 5: 143-52 https://doi.org/10.1530/ror.0.0050143
  12. Yang Y, Balla A, Danilovich N, Sairam MR. Developmental and molecular aberrations associated with deterioration of oogenesis during complete or partial follicle-stimulating hormone receptor deficiency in mice. Biol Reprod 2003; 69: 1294-302 https://doi.org/10.1095/biolreprod.103.015610
  13. McGrath SA, Esquela AF, Lee SJ. Oocyte-specific expression of growth/differentiation factor-9. Mol Endocrinol 1995; 9: 131-6 https://doi.org/10.1210/me.9.1.131
  14. Baarends WM, Uilenbroek JT, Kramer P, Hoegerbrugge JW, van Leeuwen EC, Themmen AP, Grootegoed JA. Anti-Mullerian hormone and anti-Mullerian hormone type II receptor messenger ribonucleic acid expression in rat ovaries during postnatal development, the estrous cycle, and gonadotropininduced follicle growth. Endocrinology 1995; 136: 4951-62 https://doi.org/10.1210/en.136.11.4951
  15. Durlinger AL, Kramer P, Karels B, de Jong FH, Uilenbroek JT, Grootegoed JA, Themmen AP. Control of primordial follicle recruitment by antiMullerian hormone in the mouse ovary. Endocrinology 1999; 140: 5789-96 https://doi.org/10.1210/en.140.12.5789
  16. Durlinger AL, Gruijters MJ, Kramer P, Karels B, Kumar TR, Matzuk MM, Rose UM, de Jong FH, Uilenbroek JT, Grootegoed JA, Themmen AP. AntiMullerian hormone attenuates the effects of FSH on follicle development in the mouse ovary. Endocrinology 2001; 142: 4891-9 https://doi.org/10.1210/en.142.11.4891
  17. Durlinger AL, Gruijters MJ, Kramer P, Karels B, Ingraham HA, Nachtigal MW, Uilenbroek JT, Grootegoed JA, Themmen AP. Anti-Mullerian hormone inhibits initiation of primordial follicle growth in the mouse ovary. Endocrinology 2002; 143: 1076-84 https://doi.org/10.1210/en.143.3.1076
  18. Weenen C, Laven JS, Von Bergh AR, Cranfield M, Groome NP, Visser JA, Kramer P, Fauser BC, Themmen AP. Anti-Mullerian hormone expression pattern in the human ovary: potential implications for initial and cyclic follicle recruitment. Mol Hum Reprod 2004; 10: 77-83 https://doi.org/10.1093/molehr/gah015
  19. Dierich A, Sairam MR, Monaco L, Fimia GM, Gansmuller A, LeMeur M, Sassone-Corsi P. Impairing follicle-stimulating hormone (FSH) signaling in vivo: targeted disruption of the FSH receptor leads to aberrant gametogenesis and hormonal imbalance. Proc Natl Acad Sci USA 1998; 95: 13612-13617
  20. Elvin JA, Yan C, Wang P, Nishimori K, Matzuk MM. Molecular characterization of the follicle defects in the growth differentiation factor 9-deficient ovary. Mol Endocrinol 1999; 13: 1018-34 https://doi.org/10.1210/me.13.6.1018
  21. Dong J, Albertini DF, Nishimori K, Kumar TR, Lu N, Matzuk MM. Growth differentiation factor-9 is required during early ovarian folliculogenesis. Nature 1996; 383: 531-5 https://doi.org/10.1038/383531a0
  22. Bodensteiner KJ, Clay CM, Moeller CL, Sawyer HR. Molecular cloning of the ovine Growth/Differentiation factor-9 gene and expression of growth/differentiation factor-9 in ovine and bovine ovaries. Biol Reprod 1999; 60: 381-6 https://doi.org/10.1095/biolreprod60.2.381
  23. Aaltonen J, Laitinen MP, Vuojolainen K, Jaatinen R, Horelli-Kuitunen N, Seppa L, Louhio H, Tuuri T, Sjoberg J, Butzow R, Hovata O, Dale L, Ritvos O. Human growth differentiation factor 9 (GDF-9) and its novel homolog GDF-9B are expressed in oocytes during early folliculogenesis. J Clin Endocrinol Metab 1999; 84: 2744-50 https://doi.org/10.1210/jc.84.8.2744
  24. Teixeira Filho FL, Baracat EC, Lee TH, Suh CS, Matsui M, Chang RJ, Shimasaki S, Erickson GF. Aberrant expression of growth differentiation factor9 in oocytes of women with polycystic ovary syndrome. J Clin Endocrinol Metab 2002; 87: 1337-44 https://doi.org/10.1210/jc.87.3.1337
  25. Yoon SJ, Lee KA, Ko JJ, Cha KY. Expression of growth differentiation factor-9 in the mouse ovaries at different developmental stages. Dev Reprod 1999; 3: 95-100
  26. Romero C, Paredes A, Dissen GA, Ojeda SR. Nerve growth factor induces the expression of functional FSH receptors in newly formed follicles of the rat ovary. Endocrinology 2002; 143: 1485-94 https://doi.org/10.1210/en.143.4.1485