Browse > Article
http://dx.doi.org/10.14348/molcells.2014.2288

The Up-Regulation of miR-199b-5p in Erythroid Differentiation Is Associated with GATA-1 and NF-E2  

Li, Yuxia (Department of Biochemistry, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences (CAMS) and Peking Union Medical College (PUMC), National Laboratory of Medical Molecular Biology)
Bai, Hua (Department of Ophthalmology, The Military General Hospitalof Beijing PLA)
Zhang, Zhongzu (Department of Biochemistry, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences (CAMS) and Peking Union Medical College (PUMC), National Laboratory of Medical Molecular Biology)
li, Weihua (Henan University Hospital of Huaihe Henan University Clinical College KaiFeng)
Dong, Lei (Department of Biochemistry, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences (CAMS) and Peking Union Medical College (PUMC), National Laboratory of Medical Molecular Biology)
Wei, Xueju (Department of Biochemistry, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences (CAMS) and Peking Union Medical College (PUMC), National Laboratory of Medical Molecular Biology)
Ma, Yanni (Department of Biochemistry, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences (CAMS) and Peking Union Medical College (PUMC), National Laboratory of Medical Molecular Biology)
Zhang, Junwu (Department of Biochemistry, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences (CAMS) and Peking Union Medical College (PUMC), National Laboratory of Medical Molecular Biology)
Yu, Jia (Department of Biochemistry, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences (CAMS) and Peking Union Medical College (PUMC), National Laboratory of Medical Molecular Biology)
Sun, Guotao (Institute of Molecular Medicine, Medical School, Henan University)
Wang, Fang (Department of Biochemistry, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences (CAMS) and Peking Union Medical College (PUMC), National Laboratory of Medical Molecular Biology)
Publication Information
Molecules and Cells / v.37, no.3, 2014 , pp. 213-219 More about this Journal
Abstract
MicroRNAs (miRNAs) represent a class of small non-coding regulatory RNAs that play important roles in normal hematopoiesis, including erythropoiesis. Although studies have identified several miRNAs that regulate erythroid commitment and differentiation, we do not understand the mechanism by which the crucial erythroid transcription factors, GATA-1and NF-E2 directly regulate and control differentiation via miRNA pathways. In this study, we identified miR-199b-5p as a key regulator of human erythropoiesis, and its expression was up-regulated during the erythroid differentiation of K562 cells. Furthermore, the increase of miR-199b-5p in erythroid cells occurred in a GATA-1- and NF-E2-dependent manner during erythrocyte maturation. Both GATA-1 and NF-E2 bound upstream of the miR-199b gene locus and activated its transcription. Forced expression of miRNA-199b-5p in K562 cells affected erythroid cell proliferation and maturation. Moreover, we identified c-Kit as a direct target of miR-199b-5p in erythroid cells. Taken together, our results establish a functional link among the erythroid transcription factors GATA-1/NF-E2, miR-199b-5p and c-Kit, and provide new insights into the coupling of transcription and post-transcription regulation in erythroid differentiation.
Keywords
c-Kit; erythroid differentiation; GATA-1; microRNA-199b-5p; NF-E2;
Citations & Related Records
연도 인용수 순위
  • Reference
1 D'Allard, D., Gay, J., Descarpentries, C., Frisan, E., Adam, K., Verdier, F., Floquet, C., Dubreuil, P., Lacombe, C., Fontenay, M., et al. (2013). Tyrosine kinase inhibitors induce down-regulation of c-kit by targeting the ATP pocket. PLoS One 8, e60961.   DOI
2 Azzouzi, I., Moest, H., Winkler, J., Fauchere, J.C., Gerber, A.P., Wollscheid, B., Stoffel, M., Schmugge, M., and Speer, O. (2011). MicroRNA-96 directly inhibits c-Globin expression in human erythropoiesis. PLoS One 6, e22838.   DOI
3 Bellon, M., Lepelletier, Y., Hermine, O., and Nicot, C. (2009). Deregulation of microRNA involved in hematopoiesis and the immune response in HTLV-I adult T-cell leukemia. Blood 113, 4914-4917.   DOI   ScienceOn
4 Bousquet, M., Harris, M.H., Zhou, B.Y., and Lodish, H.F. (2010). MicroRNA miR-125b causes leukemia. Proc. Natl. Acad. Sci. USA 107, 21558-21563.   DOI   ScienceOn
5 Bruchova, H., Merkerova, M., and Prchal, J.T. (2008). Aberrant expression of microRNA in polycythemia vera. Haematologica 93, 1009-1016.   DOI   ScienceOn
6 Fang, C., Zhao, Y., and Guo, B. (2013). MiR-199b-5p targets HER2 in breast cancer cells. J. Cell. Biochem. 114, 1457-63.   DOI   ScienceOn
7 Faraoni, I., Laterza, S., Ardiri, D., Ciardi, C., Fazi, F., and Lo-Coco, F. (2012). MiR-424 and miR-155 deregulated expression in cytogenetically normal acute myeloid leukaemia: correlation with NPM1 and FLT3 mutation status. J. Hematol. Oncol. 5, 26.   DOI   ScienceOn
8 Flamant, S., Ritchie, W., Guilhot, J., Holst, J., Bonnet, M.L., Chomel, J.C., Guilhot, F., Turhan, A.G., and Rasko, J.E. (2010). Micro-RNA response to imatinib mesylate in patients with chronic myeloid leukemia. Haematologica 95, 1325-1333.   DOI
9 Fukao, T., Fukuda, Y., Kiga, K., Sharif, J., Hino, K., Enomoto, Y., Kawamura, A., Nakamura, K., Takeuchi, T., and Tanabe, M. (2007). An evolutionarily conserved mechanism for microRNA-223 expression revealed by microRNA gene profiling. Cell 129, 617-631.   DOI   ScienceOn
10 Garzia, L., Andolfo, I., Cusanelli, E., Marino, N., Petrosino, G., Martino, D.D., Esposito, V., Galeone, A., Navas, L., and Esposito, S. (2009). MicroRNA-199b-5p impairs cancer stem cells through negative regulation of HES1 in medulloblastoma. PLoS One 4, e4998.   DOI   ScienceOn
11 Grabher, C., Payne, E.M., Johnston, A.B., Bolli, N., Lechman, E., Dick, J.E., Kanki, J.P., and Look, A.T. (2011). Zebrafish micro-RNA-126 determines hematopoietic cell fate through c-Myb. Leukemia 25, 506-514.   DOI   ScienceOn
12 Heuston, E.F., Lemon, K.T., and Arceci. R.J. (2011). The beginning of the road for non-coding RNAs innormal hematopoiesis and hematologic malignancies. Front Genet. 2, 94.
13 Navarro, F., and Lieberman, J. (2010). Small RNAs Guide Hematopoietic Cell Differentiation and Function. J. Immunol. 184, 5939-5947.   DOI   ScienceOn
14 Pase, L., Layton, J.E., Kloosterman, W.P., Carradice, D., Waterhouse, P.M., and Lieschke, G.J. (2009). miR-451 regulates zebrafish erythroid maturation in vivo via its target gata2. Blood 113, 1794-1804.   DOI   ScienceOn
15 Wang, F., Yu, J., Yang, G.H., Wang, X.S., and Zhang, J.W. (2011). Regulation of erythroid differentiation by miR-376a and its targets. Cell Res. 21, 1196-1209.   DOI   ScienceOn
16 Wu, X.W., Weng, L.H., Li, X.J., Guo, C., Pal, S.K., Jin, J.M., Li, Y.P., Nelson, R.A., Mu, B., Onami, S.H., et al. (2012). Identification of a 4-microRNA Signature for clear cell renal cell carcinoma metastasis and prognosis. PLoS One 7, e35661.   DOI
17 Andolfo, I., Liguori, L., De, A.P., Cusanelli, E., Marinaro, F., Pistollato, F., Garzia, L., De, V.G., Petrosino, G., Accordi, B., et al. (2012). The micro-RNA 199b-5p regulatory circuit involves Hes1, CD15, and epigenetic modifications in medulloblastoma. Neuro Oncol. 14, 596-612.   DOI
18 Yu, D., dos Santos, C.O., Zhao, G., Jiang, J., Amigo, J.D., Khandros, E., Dore, L.C., Yao, Y., D'Souza, J., Zhang, Z., et al. (2010). miR-451 protects against erythroid oxidant stress by repressing 14-3-3zeta. Genes Dev. 24, 1620-1633.   DOI   ScienceOn
19 Zhang, L.B., Flygare, J., Wong, P., Lim, B., and Lodish, HF. (2010). miR-191 regulates mouse erythrob last enucleation by downregulating Riok3 and Mxi1. Genes Dev. 25, 119-124.
20 Zhu, Y., Wang, D.S., Wang, F., Li, T.T., Dong, L., Liu, H.W., Ma, Y.N., Jiang, F.B., Yin, H.X., Yan, W.T., et al. (2013). A comprehensive analysis of GATA-1-regulated miRNAs reveals miR-23a to be a positive modulator of erythropoiesis. Nucleic Acids Res. 41, 4129-4143.   DOI   ScienceOn
21 Huang, X., Gschweng, E., Van, H.B., Cheng, D., Mikkola, H.K., and Witte, O.N. (2011). Regulated expression of microRNAs-126/126* inhibits erythropoiesis from human embryonic stem cell. Blood 117, 2157-2165.   DOI   ScienceOn
22 Yu, J., Ryan, D.G., Getsios, S., Oliveira-Fernandes, M., Fatima, A., and Lavker, R.M. (2008). MicroRNA-184 antagonizes micro-RNA-205 to maintain SHIP2 levels in epithelia. Proc. Natl. Acad. Sci. USA 105, 19300-19305.   DOI   ScienceOn