• 한국자원식물학회지
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• 제25권3호
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• pp.357-362
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• 2012

The potential cultivation of buckwheat species in Luang Prabang region, Lao PDR where the species has not been naturally grown and not traditionally utilized was studied to introduce buckwheat as a new agricultural material, especially for food and medicine. The growth of Tartary buckwheat species was higher than common buckwheat showing 2.3 fold of plant height compared to common buckwheat. Number of flower per plant in Tartary buckwheat (731) was three fold higher than common buckwheat (244). Dry weight per plant was respectively 1.7 g in Suwon No. 1, 2.6 g in Suwon No. 2, and 1.4 g in KW45. The rate average of seed setting was similar between common buckwheat (41 to 46 %) and Tartary buckwheat (44%). Total seed yield of Tartary buckwheat (184 kg/10 a) in 10a could be estimated to be higher than that of common buckwheat (108 kg/10 a).

• 한국자원식물학회지
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• 제22권3호
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• pp.227-235
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• 2009

Single seeds of common buckwheat cultivar Suwon No. 1 when subjected to SDS-PAGE revealed very high polymorphism. High variation existed for protein or protein subunits with molecular weight 54-47kDa, 45-25kDa and 16-11kDa. The electrophoregram showed variation for globulin as well as other protein fractions. About 300 proteins were separated by two-dimensional electrophoresis in common buckwheat (Fagopyrum esculentum Moench.) seed. Seed maturation is a dynamic and temporally regulated phase of seed development that determines the composition of storage proteins reserves in mature seeds. Buckwheat seeds from 5, 10, 15, 20, and 25 days after pollination and matured stage were used for the analysis. This led to the establishment of high-resolution proteome reference maps, expression profiles of 48 spots. It was identified 48 proteins from MALDI-TOF/MS analysis of wild buckwheat seed storage proteins. The 48 proteins were found identical or similar to those of proteins reported in buckwheat and other plants; it is belonging to 9 major functional categories including seed storage proteins, stress/defense response, protein synthesis, photosynthesis, allergy proteins, amino acid, enzyme, metabolism, and miscellaneous. It appears that the major allergenic storage protein separated played the important role in buckwheat breeding and biochemical characterization.

• 한국자원식물학회지
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• 제18권2호
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• pp.246-250
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• 2005

아시아 지역에서 수집한 야생 쓴메밀의 종자 및 식물체 내의 루틴함량을 조사하였다. 쓴메밀은 종자 및 식물 전체부위에서 숙근메밀이나 단메밀보다 높은 루틴 함량을 나타냈다. 특히, 꽃은 숙근메밀의 2.2배, 단메밀의 9.5배, 줄기는 각각 3.1배와 24.9배, 종자는 3.2와 65배나 높았다. 3종에 대한 식물체 부위별 루틴함량은 모두 꽃>잎>종자>줄기>뿌리의 순으로 높았다. 종피색에 따른 루틴함량은 암회색>검은색>갈색, 회갈색 순의 경향을 나타냈으며, 종자의 형태는 Slender>Notched>Round 순으로 루틴함량이 높았다. 재배종은 야생종에 비해 루틴함량이 잎은 1.3배, 줄기가 1.4배, 종자가 1.2배의 높은 경향을 보였다. 부탄지역 수집품종은 잎, 줄기, 종자의 루틴함량이 다른 지역보다 높은 경향을 보였고, 슬로베니아 및 파키스탄지역 수집품종은 잎, 줄기에서, 인도 및 네팔지역 수집품종은 종자에서 가장 낮은 루틴함량의 경향을 보였다.

• 한국자원식물학회지
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• 제29권6호
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• pp.712-721
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• 2016

This experiment was conducted to investigate the endophytic bacterium Herbaspirillum spp effect on seed germination and sprout growth of tartary buckwheat. Inoculant concentration (%v/v) and seed soaking time were applied 10, 20 and 40% and 0, 4, 8, 12 hour, respectively. The experiment was carried out in a growth chamber maintained temperature at 20, 25 and $30^{\circ}C$ without light for 7 days. Results showed that, 10 to 20% (v/v) inoculant concentration by 4 to 8 h seed soaking time at $20^{\circ}C$ temperature increased seed vigor rate and total seed germination rate 80-95% and 90-100%, respectively. On the other and, seed inoculation with Herbaspirillum spp. increased hypocotyl length (13-15 cm), root length (8-11 cm), total fresh weight (135-296 g) and total dry weight (7-10 g), compared to control. It is indicated that sprouts growth and yield depends on inoculation concentrations, seed soaking time and temperature. Therefore, it would be suggested that seed inoculation with Herbaspirillum spp. at concentration of 10 to 20% (v/v), soaking time 4 to 8 h and temperature $20^{\circ}C$ promote seed germinations and sprout growth rate of tartary buckwheat.

• 한국식품영양과학회지
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• 제34권1호
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• pp.81-86
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• 2005

메밀종자와 메밀나물의 영양성분 및 유용성분을 비교 분석하여, 식품개발 소재로서 메밀나물의 이용성을 증대시키는 기초자료로 삼고자 하였다. 메밀종자를 7일간 발아시켜 메밀나물을 수확한 후, 동결건조하여 분석용 시료로 사용하였다. 건물량 기준으로 메밀나물의 조단백, 조지방, 회분함량은 각각 20.8, 1.3 그리고 2.6%이었다. 메밀나물의 주된 아미노산은 glutamic acid(2,764 mg/100 g)와 aspartic acid(1,698 mg/100 g)이었다. 발아를 통해 tryptophan이 약 1.9배, alanine과 tyrosine이 약 1.8배, histidine이 약 1.7배정도 증가하는 것으로 나타났다. 메밀나물의 주된 지방산은 iinoleic acid(45.9%)와 oleic acid(18.4%)이었다. 메밀종자가 발아되어 메밀나물로 성장하는 가운데 stearic acid(18:0)가 21%, oleic acid(18:1)가 약 50% 감소된 반면 linoleic acid(18:2)와 linolenic acid(18:3)가 각각 1.3배,5.4배 증가하는 현상을 나타내었다 메밀나물의 칼슘함량은 152.0 mg/100 g, 아연 9.9 mg/100 g, 마그네슘 485.0 mg/100 g, 철분 5.4 mg/100 g이었다. 메밀나물의 비타민 A, C and E 함량은 건물량 기준으로 각각 1,180 IU/100 g, 203 mg/100 g 그리고 32.1 mg/100 g이었다. 특히 $\alpha$-tocopherol 함량은 메밀종자에 비해 27.5배나 높았다. 메밀나물의 rutin 함량은 343.67 mg/100 g로 메밀종자보다 약 18배 많은 것으로 나타났다.

• 한국작물학회지
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• 제60권1호
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• pp.29-32
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• 2015

Two-dimensional electrophoresis (2-DE) was executed to separate the seed storage proteins from the buckwheat. The proteins extracted from the whole seed proteins were better separated and observed in the use of lysis buffer. Using this method, the highly reproducible isoelectric focusing (IEF) can be obtained from polyacrylamide gels, and IEF from the polyacrylamide gel at all the possible pH range (5.0-8.0) was more easily separated than IPG (immobilized pH gradient) gels. The polyacrylamide gels in the first dimension in 2-DE was used to separate and identify a number of whole seed proteins in the proteome analysis. In this new apparatus using 2-DE, 27cm in length of plate coated with polyacrylamide gel was used and the experiment was further investigated under the various conditions.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.76-76
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• 2017

Buckwheat has gained scientists concern due to its nutritional and medicinal values in recent years, and many important bioactive compounds. The present study was performed to investigate the influence of the varietal performance and environmental conditions on the content of rutin and quercetin in Tartary buckwheat germplasm. A total of 44 foreign Tartary buckwheat germplasms were examined and compared their contents based on the collected countries, seed shape, and seed color. The highest number of germplasm (16) was found in ranged from 600 mg/100 g to 800 mg/100 g of rutin content. In case of quercetin content, the highest number of germplasm (19) was observed in ranged from 5 mg/100 g to 10 mg/100 g. However, the rutin content showed the highest value (1326.6 mg/100 g) from CBU408 and quercetin content showed the highest value (22.5 mg/100 g) from CBU456. The number of germplasm showed the highest value (23) in ranged from 3000 mg/100 g to 4000 mg/100 g of rutin content. The number of germplasm is the highest value (30) in ranged from 100 mg/100 g to 200 mg/100 g of quercetin content. When rutin and quercetin content in seed was compared according to collected countries, seed shape, and seed color respectively, no association was appeared with rutin and quercetin content. In sprout of whole Tartary buckwheat germplasm, mean of rutin content and quercetin content was 3362.9 mg/100 g and 14.2 mg/100 g respectively. In conclusion, the results observed from the present study suggest that highly rutin germplasm could be used more efficiently via breeding to develop a highly rutin content variety for using in sprout. No correlation was appeared in the case of quercetin. However, the quercetin content in sprout was 4~90 times more than seed. So, sprout could be used more efficiently than seed in the case of quercetin. Our results suggest that the varietal variation and qualitative characteristic differences of rutin and bioactive compounds may provide important nutrient sources and industrial application of Tartary buckwheat.

• Plant Resources
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• 제5권1호
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• pp.45-50
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• 2002

Korean cultivars of buckwheat(Fagopyrum esculentum Moench.) was crossed with Canadian cultivars in order to improve seed yield as well as leaf production of buckwheat for using as food and medicine. The agronomic characteristics and rutin contents of F$_2$ hybrids are investigated for further selection of superior lines. Dry weight per plant was the highest in a line 1110(6.71g) and leaf weight per plant was the highest in a line 1110(1.91g). Hybrid seeds were 0.55 - 0.70cm long and 0.37 - 0.47cm wide on average. 100 seeds weight ranged from 2.57g to 3.58g. Line 1076 produced the longest seeds(0.70cm) and line 1186 was the longest in seed width. Line 1196 showed the highest 100 seeds weight(3.58g). The highest frequency of the LWR(length/width rate) was 0.66~0.70, indicating that seed shape of the hybrids was mostly oval. Line 1087 showed the highest contents of rutin(77.26ppm). Lines 1090 and 1181 contained respectively rutin of 54.76ppm and 54.35ppm in the seeds. From the yield and rutin point of view, the most superior lines was line 1087 among the lines used for this study.

• 한국작물학회지
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• 제50권spc1호
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• pp.175-180
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• 2005

본 연구는 단메밀과 타타리메밀의 페놀성화합물의 함량을 측정하고 분석, 평가한으로써 생리활성 연구의 기초 자료와 건강기능성 식품소재 개발에도 이용될 수 있는 유용한 기초 자료를 얻고자 수행하였다. 1. 총 페놀의 함량은 타타리메밀 종자가 단메밀 종자보다 약 2배정도 높게 나타났다. 총 페놀 중의 플라보노이드 함량은 단메밀이 약 $50\%$, 타타리메밀이 약 $95\%$를 차지하고 있다. 단메밀과 타타리메밀의 총 페놀 및 플라보노이드 함량은 메밀쌀이 메밀껍질보다 높았으나, 단메밀의 플라보노이드 함량은 메밀껍질 더 높았다. 3. 단메밀의 rutin함량은 메밀껍질(25.2 mg/100g)>껍질을 벗기지 않은 종자(19.8 mg/100 g)>메밀쌀(12.8 mg/100 g)의 순으로 높았으나, 타타리메밀은 메밀쌀(2042.1mg/100g)>껍질을 벗기지 않은 종자(1375.8 mg/100g)>메밀껍질(138.7 mg/100 g)의 순으로 높게 나타났다. 식물체 부위별 rutin의 함량은 단메밀이 메밀싹>잎>줄기>종자의 순이고, 타타리메밀은 잎enli메밀싹>종자>줄기의 순으로 나타났다. 모든 부위에서 타타리메밀이 단메밀보다 높은 rutin함량을 나타냈다. 4. 단메밀과 타타리메밀 종자의 flavanols함량은 큰 차이를 보이지 않았으나, 잎, 줄기, 싹나물부위의 함량은 타타리메밀이 높은 경향을 보였고, 두 종의 flavonols의 함량도 단메밀보다 타타리메밀에서 월등히 높은 경향을 보였다. 5. 식물체 부위별 flavanols의 함량은 단메밀과 타타리메밀의 메밀싹>잎>줄기>종자의 순으로 높게 나타났다. 모든 부위에서 catechin> Epicatechin> Epicatechingallate의 순으로 높게 나타났으나, 메밀싹은 두 종 모두 Epicatechingallate의 함량이 종자의 약 $30\~40$배, 잎, 줄기보다 약 $15\~20$배가량 높았다.

• 한국작물학회지
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• 제32권4호
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• pp.443-446
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• 1987

들깨 및 메밀 건경의 물 추출액을 피 및 배추에 처리하여 발아 및 초기생장의 억제 양상을 본 결과 1. 두가지 추출액 모두 피와 배추의 발아를 억제하였으며 메밀 추출액이 들깨 추출액보다 심하였고 피보다는 배추에 억제효과가 켰다. 2. 발아후 초기 생장도 위와 같은 경향이나 발아억제보다는 생장 억제 효과가 약간 적은 영향이었다. 3. 이들 추출액은 지상부 생장보다는 뿌리 생장에 피해를 더 주는 경향이며 들깨 추출액보다 메밀 추출액이 더 심한 편이었다.