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http://dx.doi.org/10.7740/kjcs.2014.60.1.029

Proteome Approach as a Tool for the Efficient Separation of Seed Storage Proteins from Buckwheat  

Cho, Seong-Woo (Dept. of Crop Science, Chungbuk National University)
Kwon, Soo-Jeong (Dept. of Crop Science, Chungbuk National University)
Roy, Swapan Kumar (Dept. of Crop Science, Chungbuk National University)
Woo, Sun-Hee (Dept. of Crop Science, Chungbuk National University)
Publication Information
KOREAN JOURNAL OF CROP SCIENCE / v.60, no.1, 2015 , pp. 29-32 More about this Journal
Abstract
Two-dimensional electrophoresis (2-DE) was executed to separate the seed storage proteins from the buckwheat. The proteins extracted from the whole seed proteins were better separated and observed in the use of lysis buffer. Using this method, the highly reproducible isoelectric focusing (IEF) can be obtained from polyacrylamide gels, and IEF from the polyacrylamide gel at all the possible pH range (5.0-8.0) was more easily separated than IPG (immobilized pH gradient) gels. The polyacrylamide gels in the first dimension in 2-DE was used to separate and identify a number of whole seed proteins in the proteome analysis. In this new apparatus using 2-DE, 27cm in length of plate coated with polyacrylamide gel was used and the experiment was further investigated under the various conditions.
Keywords
Buckwheat; 2-DE; Efficient Separation Method; Seed Storage Protein; Proteome Approach;
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