• 제목/요약/키워드: broccoli

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Antimutagenic Effect of Insoluble Dietary Fibers from Some Green Yellow Vegatagles and Soybean by Binding the Carcinogens (녹황색채소류 및 대두에서 분리한 불용성 식이섬유의 항돌연변이 효과)

  • Lee, Seon-Mi;Rhee, Sook-Hee;Park, Kun-Young;Rh-ew, Tae-Hyong;Kim, Byeong-Gee;Chung, Hae-Young
    • Journal of Life Science
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    • v.5 no.1
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    • pp.26-32
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    • 1995
  • The antimutagenic effects of insoluble dietary fibers(IDF) extracted from some green-yellow vegetables(kale, carrot, spinach, broccoli and soybean sprout) and soybean by binding the carcinogens of MeIQ (2-amino-3,4- dimethyl-imidazo(4,5-f) quinoline) and Trp-P-2(3-amino-1-methyl-5H-pyrido[4,3-b] indole) in Salmonella tylhimirium TA100 and TA98 were studied. All of the insoluble dietary fiber samples which binded MeIQ exhibited high antimutagenic effects by removing the mutagen. Among the samples, IDFs from kale and soybean showed strong binding capacity fo the carcinogen and revealed about 90% of the antimutagenic activity. the IDF samples showed somewhat lower binding capacity to the Trp-p-2. The lignin which extracted from kale, soybean and carrot, and the cellulose strongly removed the mutagenicity of MeIQ by the binding. Among the samples, the level of lignin in kale revealed the highest(about 10%), and it seemed that the higher content of lignin in kale is one of the reasons to increase its antimutagenic effect.

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Effect of Kaempferol on Modulation of Vascular Contractility Mainly through PKC and CPI-17 Inactivation

  • Hyuk-Jun Yoon;Heui Woong Moon;Young Sil Min;Fanxue Jin;Joon Seok Bang;Uy Dong Sohn;Hyun Dong Je
    • Biomolecules & Therapeutics
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    • v.32 no.3
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    • pp.361-367
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    • 2024
  • In this study, we investigated the efficacy of kaempferol (a flavonoid found in plants and plant-derived foods such as kale, beans, tea, spinach and broccoli) on vascular contractibility and aimed to clarify the detailed mechanism underlying the relaxation. Isometric contractions of divested muscles were stored and linked with western blot analysis which was carried out to estimate the phosphorylation of myosin phosphatase targeting subunit 1 (MYPT1) and phosphorylation-dependent inhibitory protein for myosin phosphatase (CPI-17) and to estimate the effect of kaempferol on the RhoA/ROCK/CPI-17 pathway. Kaempferol conspicuously impeded phorbol ester-, fluoride- and a thromboxane mimetic-derived contractions regardless of endothelial nitric oxide synthesis, indicating its direct effect on smooth muscles. It also conspicuously impeded the fluoride-derived elevation in phospho-MYPT1 rather than phospho-CPI-17 levels and phorbol 12,13-dibutyrate-derived increase in phospho-CPI-17 and phospho-ERK1/2 levels, suggesting the depression of PKC and MEK activities and subsequent phosphorylation of CPI-17 and ERK1/2. Taken together, these outcomes suggest that kaempferol-derived relaxation incorporates myosin phosphatase retrieval and calcium desensitization, which appear to be modulated by CPI-17 dephosphorylation mainly through PKC inactivation.

Antiproliferative Activity of Vegetable Soup in Human Cancer cells for Wellness Convergence (융복합적인 웰리스를 위한 야채수프의 인간 암세포 증식 억제효과)

  • Sim, Jae-Geun;Lee, Jae-Hyeok;Park, Jeong-Suk
    • Journal of Digital Convergence
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    • v.13 no.8
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    • pp.543-548
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    • 2015
  • The present sturdy was designed to determine the effect of the antiproliferation in human cancer cells using the ordinary vegetable soup (VS), the soup with broccoli (VSB) and the soup with tomatoes(VST). Human cancer cells identify the cancer cell growth with MTS, using stomach cancer cell line(AGS), human promyelocytic leukemia (HL-60) and lung cancer cell line (A549). VSB and VST are effective on the cancer cell growth inhibition activities of AGS and have a significance compared with VS. VST has a significance with HL-60 and VSB works well in A549 more than VS. Mixed vegetable soup is considered to applicate with functional materials and using for wellness life.

A Study on Crop Group for Pesticide Efficacy and Crop Safety of Minor Crops (소면적 재배작물의 약효 및 안전성 그룹화 적용 연구)

  • Ahn, Chang-Hyun;Kim, Yong-Hun;Eom, Hoon-Sik;Lee, Gwang-Ha;Ryu, Gab-Hee
    • The Korean Journal of Pesticide Science
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    • v.18 no.4
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    • pp.364-375
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    • 2014
  • This study was carried out to investigate the pesticide efficacy and crop safety among different leafy vegetables applied with foliar spraying under greenhouse and to check extrapolating from some trial data to other minor crops. Leafy vegetables used in this study were: lettuce (Lactuca sativar), leaf broccoli (Brassica oleracea.), chicory (Cichorium intybus.), chinese cabbage (Brassica campestris subsp. napus var. pekinensis), radish (Raphanus sativus), rape (Brassica napus), crown daisy (Chrysanthemum coronarium), edible burdock (Arctium lappa), endive (Cichorium endivia) and mustard greens (Brassica jumcea). Based on the result of control efficacy, all crops were classified into the groups. The results showed the probabilities of extrapolating the control value data of minor crop within the same group. It would be possible to use the pesticides which are already been registered for similar crops to those crops have no registered pesticides.

Food purchase Behavior of Taejon Full Time Housewives with Different Education Levels (대전지역 전업주부들의 학력수준에 따른 식품구매 실태조사)

  • Kim, Kyung-Eun;Kwon, Sun-Ja;Ly, Sun-Yung
    • Journal of the Korean Society of Food Culture
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    • v.16 no.2
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    • pp.99-108
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    • 2001
  • A survey was conducted to assess the food-purchase behavior of 197 Taejon full time housewives 30 to 49 years of age. Food purchase was assessed using a questionnaire and the resulting data were analyzed using SPSS programs. Demographic data revealed that most of the households have three to five family members and that the Engel Indices of the participating households were mostly between 10 and 30%. Noticeable results of the food purchase assessment are as follows. In the grain and grain products items, the low-education group purchased more rice and barley than the high-education group, whereas the high-education group purchased more glutinous rice, brown rice, bread, macaronis and spaghetti than the low-education group. In the vegetable items, the low-education group purchased more Chinese cabbage and radish than the high-education group, while the high-education group purchased more head lettuce, broccoli and sweet pepper than the low-education group. In the meat items, the beef purchase was higher in the high-education than in the low-education group, while the pork purchase was higher in the low-education than in the high-education group . In the processed meat items, ham was the most favorite purchase item regardless of the education level. In the fish and shellfish items, Pacific cod and Alaska pollack were purchased more in the low-education than in the high-education group, and salmon and dried icefish strip were purchased more in the high-education than in the low-education group. No items in fruit group showed significant differences in the purchase amount between the low-education and the high-education group although the latter purchased more imported-fruits such as melons, kiwis, grapefruits, and oranges. Dairy products such as milk, cheese and butter were purchased more in the high-education than in the low-education group. It was also found that both the number of food items and the consumption of foods coming from animals were higher in the high-education than in the low-education group.

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Strategies of Functional Food for Cancer Prevention in Human Beings

  • Zeng, Ya-Wen;Yang, Jia-Zheng;Pu, Xiao-Ying;Du, Juan;Yang, Tao;Yang, Shu-Ming;Zhu, Wei-Hua
    • Asian Pacific Journal of Cancer Prevention
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    • v.14 no.3
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    • pp.1585-1592
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    • 2013
  • Functional food for prevention of chronic diseases is one of this century's key global challenges. Cancer is not only the first or second leading cause of death in China and other countries across the world, but also has diet as one of the most important modifiable risk factors. Major dietary factors now known to promote cancer development are polished grain foods and low intake of fresh vegetables, with general importance for an unhealthy lifestyle and obesity. The strategies of cancer prevention in human being are increased consumption of functional foods like whole grains (brown rice, barley, and buckwheat) and by-products, as well some vegetables (bitter melon, garlic, onions, broccoli, and cabbage) and mushrooms (boletes and Tricholoma matsutake). In addition some beverages (green tea and coffee) may be protective. Southwest China (especially Yunnan Province) is a geographical area where functional crop production is closely related to the origins of human evolution with implications for anticancer influence.

Sulforaphane inhibits the Th2 immune response in ovalbumin-induced asthma

  • Park, Jun-Ho;Kim, Jong-Won;Lee, Chang-Min;Kim, Yeong-Dae;Chung, Sung-Woon;Jung, In-Duk;Noh, Kyung-Tae;Park, Jin-Wook;Heo, Deok-Rim;Shin, Yong-Kyoo;Seo, Jong-Keun;Park, Yeong-Min
    • BMB Reports
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    • v.45 no.5
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    • pp.311-316
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    • 2012
  • Sulforaphane (1-isothiocyanato-4-(methylsulfinyl)-butane), belonging to a family of natural compounds that are abundant in broccoli, has received significant therapeutic interest in recent years. However, the molecular basis of its effects remains to be elucidated. In this study, we attempt to determine whether sulforaphane regulates the inflammatory response in an ovalbumin (OVA)-induced murine asthma model. Mice were sensitized with OVA, treated with sulforaphane, and then challenged with OVA. Sulforaphane administration significantly alleviated the OVA-induced airway hyperresponsiveness to inhaled methacholine. Additionally, sulforaphane suppressed the increase in the levels of SOCS-3 and GATA-3 and IL-4 expression in the OVA-challenged mice. Collectively, our results demonstrate that sulforaphane regulates Th2 immune responses. This sutdy provides novel insights into the regulatory role of sulforaphane in allergen-induced Th2 inflammation and airway responses, which indicates its therapeutic potential for asthma and other allergic diseases.

A Method for Choosing Vegetables at the Market from a General Consumers Standpoint I -The Relationship between Chlorophyll and Ascorbic Acid- (일반 소비자의 입장에서 본 시장에서의 채소류 선택 방법 I - Chlorophyll과 Ascorbic Acid 관계-)

  • Choi, Jin-Kyung;Lee, Seung-Un;Seo, Bong-Soon;Kozukue, Nobuyuki
    • Journal of the East Asian Society of Dietary Life
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    • v.17 no.5
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    • pp.671-677
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    • 2007
  • This study examined the relationship between chlorophyll(Chl) and ascorbic acid(ASA) as an index of freshness for vegetables, when the general consumers purchase several kinds of vegetables at the market. The leaves and fruits of several types of vegetables were divided into top, middle, and basal sections. Chl was determined by a spectrophotometer, and ASA was analyzed by high-performance liquid chromatography. For the vegetable sections, we found that Chl and ASA levels were highest in the top sections, followed by the middle, and were lowest in the basal portions. For the relationship between Chl and ASA in vegetables, there were seven classifications including high Chl(above 80mg/100g of fresh weight) and high ASA(above 80 mg/100 g of fresh weight) for mallow, and kail; the second group had high Chl and low ASA(below 80 mg), such as in spinach, burdock, and perilla leaves; the third group contained medium Chl($40{\sim}80mg$) and medium ASA($40{\sim}80mg$) levels, such as for gonddalbee, angelica, pumpkin leaf and coriander; the fourth group had medium Chl and low ASA levels, as in crown daisy and lettuce; the fifth group contained low Chl(below 40 mg) and high ASA, such as in hot peppers and parsley; the sixth group had low Chl and medium ASA levels, and included broccoli, pak choi, and sweet peppers; finally the seventh group contained low Chl and low ASA levels, as in dropwort, young radishes, shallots, cucumbers and swiss chard.

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Evaluation of Glucosinolate Content and Composition Contained in Korean Leaf Mustard (Brassica juncea var. integrifolia) Germplasm Using Liquid Chromatography Electrospray Ionization Mass Spectrometry(LC-ESI-MS/MS)

  • Kim, Jung-Bong;Kim, Heon-Woong;Ko, Ho-Cheol;Kim, So-Young;Kim, Se-Na;Jin, Yong-Xie;Kim, Jae-Hyun
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2010.05a
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    • pp.18-18
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    • 2010
  • Glucosinolates(GSLs) are major secondary products($\beta$-thioglucoside N-hydroxysulfates) containing sulfates that are found mainly in Cruciferae family such as cabbage, broccoli, radish, turnip, kale, mustard and rapeseed as the important crops in agronomic and economic aspects. Especially, isothiocyanates(ITCs) have been shown to inhibit carcinogenesis with regulation cancer cell development followed by regulating target enzymes, inducing cell cycle arrest and apoptosis against various human cancer cell lines. Total desulfo(DS)-GSLs of nine type in 210 Korean leaf mustard accessions were isolated and confirmed using electrospray ionization mass spectrometric(ESI-MS/MS) method and DS-GSLs powerful library maked from several reference data. The isolated individual DS-GSLs were identified by removed $\beta$-D-glucopyranose residue ($C_6H_{11}O_5$, MW 163) from MS/MS data. Among them sinigrin(41.7%), glucoiberverin(21.7%) and gluconasturtiin(12.6%) were major components, Especially, despite gluconapin was minor component, accession K046197-1 and K046197-2 showed higher content of 4.11 and 3.31 mg/g(DW), respectively. The total GSLs contents in 210 accessions were ranged from 5.3 to 23.2 mg/g(DW) with a mean value of 13.0 mg/g(DW). As a result of principal component analysis(PCA), the individual GSLs loading plots were composed of three groups and components belonged to each group showed correlationship in quantitative pattern.

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Novel Deposition Technique of ZnO:Al Transparent Conduction Oxide Layer on Chemically Etched Glass Substrates for High-haze Textured Surface

  • Park, Hyeongsik;Pak, Jeong-Hyeok;Shin, Myunghoon;Bong, Sungjae;Yi, Junsin
    • Proceedings of the Korean Vacuum Society Conference
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    • 2014.02a
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    • pp.426.1-426.1
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    • 2014
  • For high performance thin film solar cells, texturing surface, enhancing the optical absorptionpath, is pretty important. Textured ZnO:Al transparent oxide layer of high haze is commonly used in Si thin film solar cells. In this paper, novel deposition method for aluminum doped zinc oxide (ZnO:Al) on glass substrates is presented to improve the haze property. The broccoli structure of ZnO:Al layer was formed on chemically etched glass substrates, which showed high haze value on a wide wavelength range.The etching condition of the glass substrates can change not only the haze values of the ZnO:Al of in-situ growth but alsothe electrical and optical properties of the deposited ZnO:Al films.The etching mechanism of the glass substrate affecting on the surface morphology of the glass will be discussed, which resulted in variation of texture of ZnO:Al layer. The optical properties of substrate morphology were also analyzed with EDS and FTIR results. As a result, the high haze value of 85.4% was obtained in the wavelength range of 300 nm to 1100 nm. Furthermore, low sheet resistance of about 5~18 ohm/sq was achieved for different surface morphologies of the ZnO:Al films.

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