• Journal of Microbiology and Biotechnology
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• v.25 no.11
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• pp.1827-1834
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• 2015

The SMG1 lipase from Malassezia globosa is a newly found mono- and diacylglycerol (DAG) lipase that has a unique lid in the loop conformation that differs from the common alpha-helix lid. In the present study, we characterized the contribution of three residues, L103 and F104 in the lid and F278 in the rim of the binding site groove, on the function of SMG1 lipase. Site-directed mutagenesis was conducted at these sites, and each of the mutants was expressed in the yeast Pichia pastoris, purified, and characterized for their activity toward DAG and p-nitrophenol (pNP) ester. Compared with wild-type SMG1, F278A retained approximately 78% of its activity toward DAG, but only 11% activity toward pNP octanoate (pNP-C8). L103G increased its activity on pNP-C8 by approximately 2-fold, whereas F104G showed an approximate 40% decrease in pNP-C8 activity, and they both showed decreased activity on the DAG emulsion. The deletion of 103-104 retained approximately 30% of its activity toward the DAG emulsion, with an almost complete loss of pNP-C8 activity. The deletion of 103-104 showed a weaker penetration ability to a soybean phosphocholine monolayer than wild-type SMG1. Based on the modulation of the specificity and activity observed, a pNP-C8 binding model for the ester (pNP-C8, N102, and F278 form a flexible bridge) and a specific lipid-anchoring mechanism for DAG (L103 and F104 serve as "anchors" to the lipid interface) were proposed.

• Journal of Microbiology and Biotechnology
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• v.18 no.8
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• pp.1475-1481
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• 2008

B3 antibody specifically binds the $Lewis^Y$-related carbohydrate antigen of many carcinomas, and it is used as a model antibody in this study. In a previous study, the Fab fragment of the antibody was fused to a 38 kDa truncated form of Pseudomonas exotoxin A, PE38, to make Fab-PE38, where PE38 is fused to the Fd fragment of the Fab domain. This parent monomer molecule, Fab-PE38, had no cysteine in the hinge region, and it could not make a disulfide bond to form a disulfide bond bridged homodimer. In this study, we constructed three different kinds of divalent Fab-toxin fusion homodimers where the toxin is fused to the light chain of Fab, $(Fab-PE38fl)_2$. In addition to the PE38 toxin fused to the light chain, these three molecules have different hinge sequences hi, h2, and h3 making Fabh1-, Fabh2-, and Fabh3-PE38fl monomers, respectively. These hinges contain only one cysteine on different positions of the hinge sequence. The disulfide bond between the hinge region of two monomers forms homodimers $(Fabh1-PE38fl)_2$, $(Fabh2-PE38fl)_2$, and $(Fabh3-PE38fl)_2$. The refolding yields of these dimers were 5-16-fold higher than a previously constructed dimer where the PE38 was fused to the Fd fragment $(Fabh2-PE38)_2$ [8]. Our data suggest that the steric repulsion between the two PE38s in $(Fabh1-PE38)_2$ during disulfide bridge formation is relieved by fusing it at the end of the light chain. The best cytotoxicity value of these dimers showed about 2.5-fold higher on an MCF7 cell line than that of the monovalent reference molecule in ng/ml scale, which is 15-fold higher in pM scale.

• Journal of the Korean Society for Aeronautical & Space Sciences
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• v.32 no.6
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• pp.16-22
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• 2004

Unsteady vortex shedding behind a rectangular cylinder near a wall influences both increasing of drag and dynamic stability of heavy vehicle, bridge or building. Incompressible Averaged Navier-Stokes equation with modified ${\varepsilon}-SST$ turbulence model is adapted for investigating the flow field between the rectangular cylinder and the wall. In case the vortex shedding happens, not only the averaged maximum velocity is higher than other cases, but the position of the maximum velocity is closer to the lower surface of rectangular cylinder. On this study, it is confirmed that the vortex shedding behind a rectangular cylinder can be suppressed by the passive control method added by horizontal and vertical fences to the lower surface of rectangular cylinder.

• Structural Engineering and Mechanics
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• v.36 no.1
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• pp.111-127
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• 2010

This paper studies the effects of spatially varying ground motions on the responses of a bridge frame located on a canyon site. Compared to the spatial ground motions on a uniform flat site, which is the usual assumptions in the analysis of spatial ground motion variation effects on structures, the spatial ground motions at different locations on surface of a canyon site have different intensities owing to local site amplifications, besides the loss of coherency and phase difference. In the proposed approach, the spatial ground motions are modelled in two steps. Firstly, the base rock motions are assumed to have the same intensity and are modelled with a filtered Tajimi-Kanai power spectral density function and an empirical spatial ground motion coherency loss function. Then, power spectral density function of ground motion on surface of the canyon site is derived by considering the site amplification effect based on the one dimensional seismic wave propagation theory. Dynamic, quasi-static and total responses of the model structure to various cases of spatially varying ground motions are estimated. For comparison, responses to uniform ground motion, to spatial ground motions without considering local site effects, to spatial ground motions without considering coherency loss or phase shift are also calculated. Discussions on the ground motion spatial variation and local soil site amplification effects on structural responses are made. In particular, the effects of neglecting the site amplifications in the analysis as adopted in most studies of spatial ground motion effect on structural responses are highlighted.

• Smart Structures and Systems
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• v.4 no.5
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• pp.605-628
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• 2008

In bridge structures, damage may induce an additional deflection which may naturally contain essential information about the damage. However, inverse mapping from the damage-induced deflection to the actual damage location and severity is generally complex, particularly for statically indeterminate systems. In this paper, a new load concept, called the positive-bending-inspection-load (PBIL) is proposed to construct a simple inverse mapping from the damage-induced deflection to the actual damage location. A PBIL for an inspection region is defined as a load or a system of loads which guarantees the bending moment to be positive in the inspection region. From the theoretical investigations, it was proven that the damage-induced chord-wise deflection (DI-CD) has the maximum value with the abrupt change in its slope at the damage location under a PBIL. Hence, a novel damage localization method is proposed based on the DI-CD under a PBIL. The procedure may be summarized as: (1) identification of the modal flexibility matrices from acceleration measurements, (2) design for a PBIL for an inspection region of interest in a structure, (3) calculation of the chord-wise deflections for the PBIL using the modal flexibility matrices, and (4) damage localization by finding the location with the maximum DI-CD with the abrupt change in its slope within the inspection region. Procedures from (2)-(4) can be repeated for several inspection regions to cover the whole structure complementarily. Numerical verification studies were carried out on a simply supported beam and a three-span continuous beam model. Experimental verification study was also carried out on a two-span continuous beam structure with a steel box-girder. It was found that the proposed method can identify the damage existence and damage location for small damage cases with narrow cuts at the bottom flange.

• Geomechanics and Engineering
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• v.12 no.4
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• pp.723-738
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• 2017

Rock is a heterogeneous material, which introduces complexity in the analysis of rock slopes, since both the existing discontinuities within the rock mass and the intact rock contribute to the degradation of strength. Rock failure is often catastrophic due to the brittle nature of the material, involving the sliding along structural planes and the fracturing of rock bridge. This paper proposes an advanced discretization method of rock mass based on block theory. An in-house software, GeoSMA-3D, has been developed to generate the discrete fracture network (DFN) model, considering both measured and artificial joints. Measured joints are obtained from the photogrammetry analysis on the excavation face. Statistical tools then facilitate to derive artificial joints within the rock mass. Key blocks are searched to provide guidance on potential reinforcement measures. The discretized blocky system is subsequently implemented into a discontinuous deformation analysis (DDA) code. Strength reduction technique is employed to analyze the stability of the slope, where the factor of safety can be obtained once excessive deformation of slope profile is observed. The combined analysis approach also provides the failure mode, which can be used to guide the choice of strengthening strategy if needed. Finally, an illustrated example is presented for the analysis of a rock slope of 20 m height inclined at $60^{\circ}$ using combined GeoSMA-3D and DDA calculation.

• Journal of Advanced Navigation Technology
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• v.13 no.1
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• pp.79-86
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• 2009

As information communication technology developed we could monitor temperature, weather, indoor and outdoor status which we need to monitor using various sensors. Even further we could monitor our body such as Sa02 and serologic chemical tests easily at home or office. It is possible though interlocking the house medical instrument with the wireless public data network. Data from sensors can be transmitted to the distant control room and will be essentially applied through wireless public data network. In this study we measured various sensor data for the telemetry in one system. The sensing items are mainly focused on the static and dynamic behaviors of the bridge, building, instruments. The study suggests the transmit system model utilized by the wireless public data network. The suggestion in the study of telemetry system provides movement and preservation. And it will exam various condition in distance or at home.

• The Transactions of the Korean Institute of Power Electronics
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• v.3 no.3
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• pp.231-239
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• 1998

This paper presents the dynamic analysis and control loop design of a zero voltage switching full bridge (ZVS-FB) PWM DC/DC converter. The small-signal model is derived incorporating the effects of phase shift control and the utilization of transformer leakage inductance and power FET junction capacitance to achieve zero voltage resonant switching. These effects are modeled by introducing additional feedforward and feedback terms for duty cycle modulation. Based on the results of the small-signal analysis, the control loop is designed using a simple two-pole one-zero compensation circuit. To show the validity of the design procedures, the small signal analysis of the closed loop system is carried out and the potential of the zero voltage switching and the superiority of the dynamic characteristics are verified through the experiment with a 2 kW prototype converter.

• Journal of Korean Society of Steel Construction
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• v.29 no.4
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• pp.311-321
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• 2017

This paper deals with the flexural performance of a composite girder system designed to readily form a composite section without a formwork and to easily realize multistage preflexing and prestressing. After a 3-Dimensional finite element modeling for construction stage analysis, the parametric numerical analysis was performed to analyse the stress distribution on the composite girder sections and the prestressing effects along with concrete pouring method and strand tensioning method. Based on the stress distribution analysis, a favorable construction stage model has been rationally chosen and then the ultimate flexural strengths were evaluated to conduct a comparative study, which exceed the nominal flexural strength suggested by the current design specification(ASD). It can be concluded that the proposed composite girder and fabrication procedure should have a sufficient structural performance.

• Journal of Korean Society of Transportation
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• v.17 no.3
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• pp.117-130
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• 1999

Earthquakes damage roadway bridges and structures, resulting in significant impacts on transportation system Performance and regional economy. Seismic risk analysis (SRA) procedures establish retrofit priorities for vulnerable highway bridges. SRA procedures use average daily traffic volumes to determine the relative importance of a bridge. This research develops a cost-effective transportation network analysis (TAN) procedure for evaluating numerous traffic flow analyses in terms of the additional system cost due to failure. An important feature of the TNA Procedure is the use of an associative memory (AM) approach in the artificial intelligence held. A simple seven-zone network is developed and used to evaluate the TNA procedure. A subset of link failure system states is randomly selected to simulate synthetic post-earthquake network flows. The performance of different AM model is evaluated. Results from numerous link-failure scenarios demonstrate the applicability of the AM models to traffic flow estimation.