• Reproductive and Developmental Biology
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• v.34 no.1
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• pp.27-32
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• 2010

This study was conducted to estimate the effect of the breed, sire-breeds, farrowing year, farrowing season and parity on number of born alive (NBA), number of weaning (NW) and survival rates of weaning (SRW) in swine. The data were obtained from 46,704 litters of the Landrace, Yorkshire, Duroc and Cross breed farrowed from 1996 to 2005 at 142 GP are registered in Korean Animal Improvement Association (KAIA). There was highly significant effect of breed, sire breed, farrowing year, farrowing season and parity on NBA, NW, SRW (p<0.01). The result of this study could be available to genetic improvement of reproductive traits as a basic reference in Korean pig industry. To achieve the more effective improvement of reproductive traits, additional research such as genetic parameter evaluation should be performed.

• Journal of Nutrition and Health
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• v.37 no.1
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• pp.45-51
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• 2004

In this study, isoflavone (genistein, genistin, daidzein, daidzin) contents in various parts of twelve soybean cultivars and three soy sprouts were determined by high performance liquid chromatography with UV detector. Three cultivars of soybean were selected and cultured in the lab to produce sprout for five days. Total isoflavone (Total IF) varied greatly among differnt breeds of soybean in range of 99 - 649.9 $\mu\textrm{g}$/g and 522.3 - 1,277.7 $\mu\textrm{g}$/g respectively, domestic and foreign cultivars. There were greatly difference in total IF of various parts of the soybean sprouts. Sprout from the Myunjunamul-kong appeared to have 69% genistein and 22% genistin in head part, and 30% and 62% of daidzin and daidzein, respectively, in root. Meanwhile, the sprouts from Junjori contains most (84%) of daidzein in its root. Sprout from chinese black-soybean had the largest amount of genistein among the sprouts but, there were no differences in the average genistein content between three selected black and non-black soys. The glycosidic form of IF were dominant compared to aglycone forms both in soybean and sprouts by 24 times and 12 times, respectively, suggesting that during the sprouts cultivation glycosidic forms could change to aglycone forms. There are no difference in total content between genistein + genistin and daidzein + daidzin in soy and soy sprout. Therfore, considering the total IF contents, the intake of 1 soy sprout is similer to 1.5 times as soybean.

• Asian-Australasian Journal of Animal Sciences
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• v.20 no.6
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• pp.827-831
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• 2007

Myostatin is a member of the transforming growth factor-${\beta}$(TGF-${\beta}$ super-family. It acts as a negative regulator for skeletal muscle growth. Myostatin mutations are characterized by a visible, generalized increase in muscle mass in double muscled cattle breeds. To understand the biochemistry and physiology of the Chinese Yellow bovine myostatin gene, we report here for the first time expression of the gene in Escherichia coli (E. coli). Primers of the myostatin gene of Chinese Yellow Cattle were designed on the basis of the reported bovine myostatin mRNA sequence (Gen-Bank Accession No. NM005259) and optimized for E. coli codon usage. XhoI and EcoRI restriction enzyme sites were incorporated in the primers, and then cloning vector and expression vector were constructed in a different host bacterium. The expressed protein had a molecule mass of about 16 kDa as determined by SDS-PAGE under reducing conditions. The expressed protein reacted specifically with myostatin monoclonal antibody on immunoblots. Our studies should lead to the investigation of the differences in myostatin genes of various cattle and could benefit human health and food animal agriculture.

• Asian-Australasian Journal of Animal Sciences
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• v.24 no.10
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• pp.1329-1334
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• 2011

The Hinai-dori is a breed of chicken native to Akita Prefecture, Japan. An $F_2$ resource population produced by crossing low- and high-growth lines of the Hinai-dori breed was analyzed to detect quantitative trait loci (QTL) for growth traits. Highly significant QTLs for body weight at 10 and 14 weeks of age and average daily gain between 4 and 10 weeks and between 10 and 14 weeks of age were accordingly mapped in a common region between ADL0198 and ABR0287 on chromosome 1 and between MCW0240 and ABR0622 on chromosome 4, respectively. A significant QTL for body weight at 4 weeks of age and a significant QTL for average daily gain between 0 and 4 weeks of age were mapped for the first time to the same region flanking ABR0204 and ABR0284 on chromosome 1. These QTLs are good candidates for application in the development of marker-assisted selection strategies for increasing growth efficiencies in the Hinai-dori breed and native breeds of chickens in Asia.

• Journal of Veterinary Clinics
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• v.21 no.3
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• pp.264-269
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• 2004

A retrospective analysis was performed with 270 cases of canine urolithiasis examined at Laboratory of Clinical Pathology, College of Veterinary Medicine, Seoul Nat'l University in the period between January 2001 and December 2003. The Shih-Tzu (64 cases), Yorkshire terrier (60 cases), miniature schnauzer (41 cases) and maltese terrier (36 cases) had higher incidences than other breeds. Canine urolithiasis occurred from 4 months to 15 years of age, but the most prevalent age was 3 years. Mean age was $5.68\pm3.14$ years. The major mineral component. of calculi was struvite (54.1%) and calcium oxalate (30.4%). There was no sex prevalences: male 131 cases, female 132 cases. The most prevalent anatomic locations of calculi were urinary bladder (53 of 131 cases), urethra (24 of 131 cases) and bladder/urethra (45 of 131 cases) in males and urinary bladder (103 of 132 Cases) in females. The major mineral component of calculi in urinary bladder was struvite (102 of 160 cases), and that in urethra (13 of 25 cases) and multiple locations (29 of 62 cases) was calcium oxalate: The major components of calculi were not always consistent with the components which could be expected from urine pH and crystals. So it is suggested that the components of the calculi must be analyzed after surgical removal to prevent the recurrence when the calculi could not be dissolved by diets or urine pH modifiers.

• Korean Journal of Veterinary Service
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• v.32 no.3
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• pp.257-264
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• 2009

Subtyping of Brucella abortus isolates is epidemiologically important for monitoring of bovine brucellosis outbreaks. Pulsed-field gel electrophoresis (PFGE) is considered as a gold standard of molecular typing methods to study the DNA polymorphisms of bacteria. In this study, we analyzed using PFGE the DNA fragment profiles of B. abortus isolated in Gyeongbuk province from 1998 to 2006. The genomic DNA was digested with the restriction endonuclease Xba I, Xho I and Smi I followed gel electrophoresis. No distinguishable patterns of the genomic DNA digested with Xba I and Xho I were observed among the field isolates of B. abortus tested in this study. But Smi I restriction enzyme resulted in two PFGE patterns consisting of 13-15 bands that ranged in size from 33 to 668bp by standard marker. The cluster analysis by DNA fingerprinting software showed 93.75% similarity between two PFGE patterns. No different PFGE patterns were recognized among the isolates originated from various years, regions and cow breeds.

• Korean Journal of Veterinary Research
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• v.48 no.3
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• pp.375-379
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• 2008

Hips of the Golden and Labrador retrievers in Korea were evaluated with the University of Pennsylvania Hip Improvement Program (PennHIP) and the severity of joint laxity and degenerative joint disease (DJD) were compared to the PennHIP database. The distraction index (DI) of domestic Golden and Labrador retrievers was significantly higher than the DI of the PennHIP database. In the two breeds, the prevalence of DJD increased according to the DI. However, the severity of DJD did not show a positive correlation with the DI. Overweight dogs and dogs kept indoors showed more severe DJD and more prevalence of clinical signs. This report is thought to be the first case presentation of a large population of Golden and Labrador retrievers in Korea and the findings are representing the overall level of canine hip dysplasia (CHD) of domestic Retrievers, as the PennHIP method was not available in Korea until 2001. We can respect that the stock of retrievers can have a tighter hip joint through control of CHD using an accurate diagnostic method keyed to a phenotype especially concern for joint laxity using PennHIP method and an organized screening program. The clinical manifestation of dysplastic dog can be reduced through control of bodyweight and the environment with regular monitoring the hips with concern for joint laxity using PennHIP method.

• International Journal of Industrial Entomology and Biomaterials
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• v.7 no.1
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• pp.29-36
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• 2003

Phytoecdysteroids with moulting hormone (MH) activity induce different responses in silkworms when used on different day of final instar, which can be manipulated for maximum benefit like early and uniform spinning behaviour, reducing crop loss and to increase cocoon yield. The results showed that application of this hormone on seed crop viz., CSR2, CSR4 and BL44 and BL67 in early stage of 5$^{th}$ instar i.e., at 72 hrs and 96 hrs though induced early and uniform spinning behaviour, there was an adverse effect by 9-36% on the economic characters like cocoon yield, cocoon weight, cocoon shell weight and also on fecundity etc. Application of this hormone in late stage of $5^th$ instar i.e., at the onset of spinning showed non-significant variations in some of the characters like cocoon weight, cocoon shell weight, cocoon shell ratio and fecundity. The physiological implications of phytoecdysteroid in hastening the maturation events and synchronization of spinning activities in different breeds are discussed.

• Proceedings of the Korean Geotechical Society Conference
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• 2004.03b
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• pp.580-585
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• 2004

The purpose of this study is the stability evaluation of soil slope according to inclination of upper natural slope. Upper natural slope breeds loss of slope by inflow in slope of surface water by rainfal1 and f1uctuation of amount of materials in slope through method of cutting slope according to degree of inclination. Basis of standard inclination does not consider of inclination of upper natural slope and is presented uniformly. Therefore, in this study, analyzed stability of inclination of upper natural slope through limit equilibrium analysis. Result is same as following. First, safety factor through limit equilibrium analysis is almost direct decrease when gradient of soil slope is 1:1.2, 1:1.5. However, when gradient of soil slope is 1:1.0, 1:0.7, if sinclination of upper natural slope are $20^{\circ}$, it shows tendency that decrease of safety factor becomes low rapidly. Second, when when gradient of soil slope is fixed, inclination of upper natural slope increase tendency(maximum 3.0 times) that decrease of safety factor.

• Proceedings of the Korea Society of Poultry Science Conference
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• 2005.11a
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• pp.62-63
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• 2005

Korean Ogol Chicken is a natural treasure in Korea and expected to be a valuable genetics resource in the world. As an initial step to investigate the genetic structures of this breed, phylogenetic analysis has been performed using mitochondrial DNA (mtDNA) sequence variations. Total 30 Korean Ogol Chickens were investigated in this study and they were grouped into 4 haplotypes, consisting 11 birds in the largest haplotype. Based on the phylogenetic analysis, chicken breeds were divided into three major groups and Korean Ogol Chicken were appeared all of these three groups indicating their large genetic mtDNA diversity. These results will be used for making breeding and conservation strategies for this breed.