Browse > Article
http://dx.doi.org/10.5713/ajas.2007.827

Cloning and Prokaryotic Expression of the Mature Fragment of the Chinese Yellow Bovine Myostatin Gene  

Lu, Wenfa (College of Animal Science and Technology, Jilin Agricultural University)
Zhao, Jing (College of Animal Science and Technology, Jilin Agricultural University)
Wei, Guojian (Department of Veterinary Microbiology, University of Saskatchewan)
Shan, Wuesong (College of Animal Science and Technology, Jilin Agricultural University)
Publication Information
Asian-Australasian Journal of Animal Sciences / v.20, no.6, 2007 , pp. 827-831 More about this Journal
Abstract
Myostatin is a member of the transforming growth factor-${\beta}$(TGF-${\beta}$ super-family. It acts as a negative regulator for skeletal muscle growth. Myostatin mutations are characterized by a visible, generalized increase in muscle mass in double muscled cattle breeds. To understand the biochemistry and physiology of the Chinese Yellow bovine myostatin gene, we report here for the first time expression of the gene in Escherichia coli (E. coli). Primers of the myostatin gene of Chinese Yellow Cattle were designed on the basis of the reported bovine myostatin mRNA sequence (Gen-Bank Accession No. NM005259) and optimized for E. coli codon usage. XhoI and EcoRI restriction enzyme sites were incorporated in the primers, and then cloning vector and expression vector were constructed in a different host bacterium. The expressed protein had a molecule mass of about 16 kDa as determined by SDS-PAGE under reducing conditions. The expressed protein reacted specifically with myostatin monoclonal antibody on immunoblots. Our studies should lead to the investigation of the differences in myostatin genes of various cattle and could benefit human health and food animal agriculture.
Keywords
Prokaryotic Expression; Chinese Yellow Bovine; Myostatin Gene;
Citations & Related Records
Times Cited By KSCI : 3  (Citation Analysis)
Times Cited By Web Of Science : 2  (Related Records In Web of Science)
Times Cited By SCOPUS : 2
연도 인용수 순위
1 Gregory, A. M., Eley, T. C. O'Connor and T. G. R. Plomin. 2004. Etiologies of associations between childhood sleep and behavioral problems in a large twin sample. J. Am. Acad. Child. Adolesc. Psychiatry, 43:744-751.   DOI   ScienceOn
2 Grobet, L., D. Poncelet, L. J. Royo, B. Brouwers, D. Pirottin, C. Michaux, F. Menissier, M. Zanotti, S. Dunner and M. Georges. 1998 Molecular definition of an allelic series of mutations disrupting the myostatin function and causing double-muscling in cattle, Mamm. Genome. 9:210-213.   DOI   ScienceOn
3 Hyung-Joo, Jin, A. Michael, Dunn, Dulal Borthakur and Yong Soo Kim. 2004 Refolding and purification of unprocessed porcine myostatin expressed in Escherichia coli. Protein expression and purification. 35:1-10.   DOI   ScienceOn
4 Ma, X., Y. Cao, D. Shu and Y. Bi. 2005. Cloning and expression of swine myostatin gene and its application in animal immunization trial. Sci. China C Life Sci. 48:368-374.   DOI   ScienceOn
5 McPherron, A. C., A. M. Lawler and S. J. Lee. 1997. Regulation of skeletal muscle mass in mice by a new TGF-beta superfamily member. Nature 387:83-90.   DOI   ScienceOn
6 Moon, Y. S., Y. H. Yin, X. Jin, Z. S. Hong, J. S. Cho, S. C. Kim, S. K. You, D. I. Jin, J. Y. Han and Y. J. Choi. 2005. Fffect of Maternal Passive Autoimmunization against Myostatin on Growth Performance in Chickens. Asian-Aust. J. Anim. Sci. 18:1017-1021.   과학기술학회마을   DOI
7 Rong Du, Yong-Fu Chen, Xiao-Rong An, Xing-Yuan Yang, Yi Ma, Lei Zhang, Xiao-Li Yuan, Li-Mei Chen and Jian Qin 2005 Cloning and sequence analysis of myostatin promoter in sheep. The Jounal of Sequencing and Mapping 16:412-417.   DOI   ScienceOn
8 Whittemore, L.-A., K. Song, X. Li, J. Aghajanian, M. Davies, S. Girgenrath, J. J. Hill, M. Jalenak, P. Kelley, A. Knight, R. Maylor, D. O. Hara, A. Pearson, A. Quazi, S. Ryerson, X. Y. Tan, K. N. Tomkinson, G. M. Veldman, A. Widom, J. F. Wright, S. Wudyka, L. Zha and N. N. Wolfman. 2003 Inhibition of myostatin in adult mice increases skeletal muscle mass and strength, Biochem. Biophys. Res. Commun. 300:965-971.   DOI   ScienceOn
9 Y. L. Sun, M. C. Huang, C. S. Lin and Y. C. Wu. 2002 Postanal Expression of Growth/Differentation Factor-8 (GDF-8) Gene in European and Asian Pigs. Asian-Aust. J. Anim. Sci. 15:1244.   과학기술학회마을   DOI
10 Zimmers, T. A., M. V. Davies, L. G. Koniaris, P. Haynes P., A. F. Esquela, K. N. Tomkinson, A. C. McPherron, N. M. Wolfman and S. J. Lee. 2002 Induction of cachexia in mice by systemically administered myostatin. Sci. 296:1486-1488.   DOI   ScienceOn
11 Svend, K., Petersen, T. Niels and Foged. 1989. Cloning and Expression of the Pasteurella multocida Toxin Gene, toxA, in Escherichia coli Infection and immunity. 57:3907-3913.
12 Bellinge, R. H. S., D. A. Liberles, S. P. A. Laschi, P. A. O'Brien and G. K. Tay. 2005. Myostatin and its implications on animal breeding: a review. Anim. Genet. 36:1-6.   DOI   ScienceOn
13 Gray, A. M. and A. J. Mason. 1990 Requirement for activin A and transforming growth factor--beta 1 pro-regions in homodimer assembly. Science 247:1328-1330.   DOI   ScienceOn
14 Cockett, N. E., S. P. Jackson, T. L. Shay, D. Nielsen, S. S. Moore, M. R. Steele, W. Barendse, R. D. Green and M. Georges. 1994 Chromosomal localization of the callipyge gene in sheep (Ovis aries) using bovine DNA markers. Proc. Natl. Acad. Sci. USA. 91:3019-3023.   DOI
15 Carole, B., T. Mark, L. Brett, S. Mridula and K. Ravi. 2002 Single cysteine to tyrosine transition inactivates the growth inhibitory function of Piedmontese myostatin. Am. J. Physiol. Cell Physiol. 283:C135-C141.   DOI
16 Israel, D. I., J. Nove, K. M. Kerns, I. K. Moutsatsos and R. J. Kaufman. 1992. Expression and characterization of bone morphogenetic protein-2 in Chinese hamster ovary cells, Growth Factors. 7:139-150.   DOI   ScienceOn
17 Taylor ,W. E., S. Bhasin, J. Artaza, F. Byhower, M. Azam, D. H. Willard, F. C. Kull Jr. and N. Gonzalez-Cadavid. 2001. Myostatin inhibits cell proliferation and protein synthesis C2C12 muscle cells. Am. J. Physiol. Endocrinol. Metab. 280:E221-E228.   DOI
18 Sasha, B., O. B. K. Thomas, R. B. Elisabeth, D. M. Linda, W. Lisa-Anne, S. A. Rexford and S. K. Tejvir. 2002 Functional improvement of dystrophic muscle by myostatin blockade. Nature 420:418-421.   DOI   ScienceOn
19 McPherron, A. C. and S. J. Lee. 1997. Double muscling in cattle due to mutations in the myostatin gene, Proc. Natl. Acad. Sci. USA 94:12457-12461   DOI
20 Nara, H., D. Yoshizawa, H. Aso and T. Yamaguchi. 2001. Bovine myoblast differentiation during the myogenesis. Asian-Aust. J. Anim. Sci. 14:100-105.
21 Kocamis, H. and J. Killefer. 2002 Myostatin expression and possible functions in animal muscle growth, Domestic Animal Endocrinol. 23:447-454.   DOI   ScienceOn
22 Eder, J. and A. R. Fersht. 1995 Pro-sequence-assisted protein folding, Mol. Microbiol. 16:609-614.   DOI   ScienceOn
23 Thies, R. S., T. Chen, M. V. Davies, K. N. Tomkinson, A. A. Pearson, Q. A. Shakey and N. M. Wolfman. 2001. GDF-8 propeptide binds to GDF-8 and antagonizes biological activity by inhibiting GDF- 8 receptor binding, Growth Factors. 18:251-259.   DOI   ScienceOn
24 Hamrick, M. W., C. Pennington and C. D. Byron. 2003. Bone architecture and disc degeneration in the lumbar spine of mice lacking GDF-8 (myostatin). J. Orthop. Res. 21:1025-1032.   DOI   ScienceOn
25 Lee, S. J. and A. C. McPherron. 2001 Regulation of myostatin activity and muscle growth, Proc. Natl. Acad. Sci. USA 98:9306-9311.   DOI   ScienceOn
26 Kambadur, R., M. Sharma, T. P. Smith and J. J. Bass. 1997 Mutations in myostatin (GDF8) in double-muscled Belgian Blue and Piedmontese cattle, Genome. Res. 7:910-916.   DOI
27 Thomas, M., B. Langley, C. Berry, M. Sharma, S. Kirk, J. Bass and R. Kambadur. 2000. Myostatin, a negative regulator of muscle growth, functions by inhibiting myoblast proliferation, J. Biol. Chem. 275:40235-40243.   DOI   ScienceOn
28 Li, N., Y-L., Jiang, X-Fan and L-R Xiao 2002. Associations of T$\rightarrow$A Mutation in the Promoter Region of Myostatin Gene Birth Weight in Yorkshire Pigs. Asian-Aust. J. Anim. Sci. 15:1543   과학기술학회마을   DOI
29 Grobet, L., L. J. Martin and D. Poncelet. 1997 A deletion in the bovine myostatin gene cause the double-muscled phenotype in cattle. Nature Genet. 17:71-74.   DOI   ScienceOn
30 Brenig, B. and G. Brem. 1992 Genomic organization and analysis of the 5- end of the porcine ryanodine receptor gene. FEBS Lett. 298:277-279.   DOI   ScienceOn