• Title/Summary/Keyword: breeding line

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Construction of Database System on Amylose and Protein Contents Distribution in Rice Germplasm Based on NIRS Data (벼 유전자원의 아밀로스 및 단백질 성분 함량 분포에 관한 자원정보 구축)

  • Oh, Sejong;Choi, Yu Mi;Lee, Myung Chul;Lee, Sukyeung;Yoon, Hyemyeong;Rauf, Muhammad;Chae, Byungsoo
    • Korean Journal of Plant Resources
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    • v.32 no.2
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    • pp.124-143
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    • 2019
  • This study was carried out to build a database system for amylose and protein contents of rice germplasm based on NIRS (Near-Infrared Reflectance Spectroscopy) analysis data. The average waxy type amylose contents was 8.7% in landrace, variety and weed type, whereas 10.3% in breeding line. In common rice, the average amylose contents was 22.3% for landrace, 22.7% for variety, 23.6% for weed type and 24.2% for breeding line. Waxy type resources comprised of 5% of the total germplasm collections, whereas low, intermediate and high amylose content resources share 5.5%, 20.5% and 69.0% of total germplasm collections, respectively. The average percent of protein contents was 8.2 for landrace, 8.0 for variety, and 7.9 for weed type and breeding line. The average Variability Index Value was 0.62 in waxy rice, 0.80 in common rice, and 0.51 in protein contents. The accession ratio in arbitrary ranges of landrace was 0.45 in amylose contents ranging from 6.4 to 8.7%, and 0.26 in protein ranging from 7.3 to 8.2%. In the variety, it was 0.32 in amylose ranging from 20.1 to 22.7%, and 0.51 in protein ranging from 6.1 to 8.3%. And also, weed type was 0.67 in amylose ranging from 6.6 to 9.7%, and 0.33 in protein ranging from 7.0 to 7.9%, whereas, in breeding line it was 0.47 in amylose ranging from 10.0 to 12.0%, and 0.26 in protein ranging from 7.0 to 7.9%. These results could be helpful to build database programming system for germplasm management.

Evaluation and Seed Increase of Sources of Resistance to Phytophthora capsici in Pepper (고추 역병 저항성 재료의 특성평가와 종자 증식)

  • Kim, Dong-Woo;Kim, Byung-Soo;Bae, Su-Yeon;Han, Jeong-Hye
    • Current Research on Agriculture and Life Sciences
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    • v.20
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    • pp.83-90
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    • 2002
  • Resistance to Phytophthora Capsici and horticultural characteristics of selections from the resistance sources such as PI123469, PI201234, PI201232, AC2258 (=Line 29), CM334, KC268, KC358, KC820, KC821, KC822, KC823 (Line 29), KC462, KC463, KC464 were evaluated and recorded in the process of seed increase. Selections of PI123469, PI201234, PI201232, AC2258, KC823 (Line 29 = AC2258) of them showed the highest level of resistance. A considerable difference in the level of resistance was observed between lines selected from the same sources. The variation between selections derived from the same source was thought to be attributable to natural cross-pollination that may have occurred during the seed increase. Therefore, securing self-pollinated seed by wrapping the flower buds before anthesis would be necessary to obtain genetically pore resistant lines. The next alternative would be to increase the seed in small net houses for single line or field cage.

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Genetic Aspects of Persistency of Milk Yield in Boutsico Dairy Sheep

  • Kominakis, A.P.;Rogdakis, E.;Koutsotolis, K.
    • Asian-Australasian Journal of Animal Sciences
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    • v.15 no.3
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    • pp.315-320
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    • 2002
  • Test-day records (n=13677) sampled from 896 ewes in 5-9 (${\mu}$=7.5) monthly test-days were used to estimate genetic and phenotypic parameters of test-day yields, lactation milk yield (TMY), length of the milking period (DAYS) and three measures of persistency of milk yield in Boutsico dairy sheep. Τhe measures of persistency were the slope of the regression line (${\beta}$), the coefficient of variation (CV) of the test-day milk yields and the maximum to average daily milk yield ratio (MA). The estimates of variance components were obtained under a linear mixed model by restricted maximum likelihood. The heritability of test-day yields ranged from 0.15 to 0.24. DAYS were found to be heritable ($h^2$=0.11). Heritability estimates of ${\beta}$, CV and MA were 0.15, 0.13, 0.10, respectively. Selection for maximum lactation yields is expected to result in prolonged milking periods, high rates of decline of yields after peak production, variable test-day yields and higher litter sizes. Selection for flatter lactation curves would reduce lactation yields, increase slightly the length of the milking period and decrease yield variation as well as litter size. The most accurate prediction of TMY was obtained with a linear regression model with the first five test-day records.

Analysis of Changes on the Forest Environment and the Bird Community in Terms of ‘Guild’ (길드에 의한 산림환경과 조류군집 변화 분석)

  • Lee, Woo-Shin;Park, Chandra
    • The Korean Journal of Ecology
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    • v.18 no.3
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    • pp.397-408
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    • 1995
  • This study was conducted to analyze the breeding bird community by using guild concept in Mt. Baekwoon Research Forest of Seoul National University. Bird Community was studied by line transect method during the breeding seasons of birds in 1982 and its results were compared and analyzed with the Park et al. Work in 1993. Guilds were characterized by nesting and foraging sites as follows: nesting guild - (H) hole, (C) canopy, (B) bush, (E) edge; and foraging guild - (o) outside, (c) canopy, (b) bush. Bush-nesting guilds such as Tricolor Flycatcher (Ficedula zanthopygia), Blue-and-White Flycatcher (Cyanoptila cyanomelana), Stonechat (Saxicola torquata), Bush Warbler (cettia diphone) and Skylark (Alauda arvensis) had disappeared after ten years. Outside-foraging guilds such as Common Buzzard (Buteo buteo), sparrow Hawk (Accipiter nisus) and Carrion Crow (Corvus corone) also were not observed. There was a sharp decrease of species richness of bush-nesting guild, canopy-foraging guild and bush-foraging guild compared to ten years ago. These decreases indicate that forest environment of this area has been changed for the ten years, and guild concept in this study can be used usefully to elucidate the change of bird community according to the change in forest environment.

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Artificial Habitat Creation of Luciola lateralis (Coleoptera: Lamphyridae) and Research of Breeding Technique for Festival at Hwadamsup, Korea

  • Kang, Seung-Ho;Jeon, Mi-Kyung;Kwon, Soon-Jik;Na, Seok-Jong;Kim, Kyung-Ho;Jeong, Jong-Chul
    • Journal of Forest and Environmental Science
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    • v.34 no.4
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    • pp.275-283
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    • 2018
  • This study created the running water (Bandibooliwon) and lentic water (Sooryunwon) artificial habitats of Luciola lateralis in Hwadamsup, Gwangju-si, Gyunggi-do from April 2014 to February 2016. Through various habitat types, metapopulation was formed, and more sound population was induced to be maintained via interactions between habitats. We investigated coenosite at the habitat creation-target site, and 20 species in the lentic water site and 38 species in running water site were found. After releasing L. lateralis, we checked the status of settlement of L. lateralis larvae through traps and macroscopy, controlled the flow velocity of the habitats in line with the life cycle of L. lateralis (hatching, larva period, landing, pupation, copulation, and oviposition), and shaped gentle slope at water side, and planted moss. We artificially supplied melanian snails (Semisulcospira sp.), the food source, in the running water site and induced stable feeding. Also, We created artificial insectary, bred larvae stably in winter, and secured firefly population for a festival. Through night time observation, the time most suitable for a festival was confirmed to be from 20:30 to 22:00.

Proteome Analysis of Bovine Longissimus dorsi Muscle Associated with the Marbling Score

  • Shen, Y.N.;Kim, S.H.;Yoon, D.H.;Lee, H.G.;Kang, H.S.;Seo, K.S.
    • Asian-Australasian Journal of Animal Sciences
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    • v.25 no.8
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    • pp.1083-1088
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    • 2012
  • The breeding value of marbling score in skeletal muscle is an important factor for evaluating beef quality. In the present study, we investigated proteins associated with the breeding value of the marbling score for bovine sirloin to select potential biomarkers to improve meat quality through comparative proteomic analysis. Proteins isolated from muscle were separated by two-dimensional gel electrophoresis. After analyzing images of the stained gel, seven protein spots for the high marbling score group were identified corresponding to changes in expression that were at least two-fold compared to the low marbling score group. Four spots with increased intensities in the high marbling score group were identified as phosphoglycerate kinase 1, triosephophate isomerase, acidic ribosomal phosphoprotein PO, and capping protein (actin filament) Z-line alpha 2. Spots with decreased intensities in the high marbling score group compared to the low score group were identified as 14-3-3 epsilon, carbonic anhydrase II, and myosin light chain 1. Expression of myosin light chain 1 and carbonic anhydrase 2 was confirmed by Western blotting. Taken together, these data could help improve the economic performance of cattle and provide useful information about the underlying the function of bovine skeletal muscle.

An Analysis of Heterosis in the Silkworm, Bombyx mori (L.)

  • Singh, Tribhuwan;Saratchandra, Beera;Murthy, Geetha N.
    • International Journal of Industrial Entomology and Biomaterials
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    • v.5 no.1
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    • pp.23-32
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    • 2002
  • The introduction of hybrid and exploitation of heterosis has played a vital role in Indian sericulture industry, which clearly depicts a quantum jump in silk production during the last four decades. Since, the introduction of heterosis, progress in silkworm breeding has depended on success or failure in identifying better combiners. Systematic procedures developed have enabled the breeders to identify the best combiners by combining ability test, line ${\TIMES}$ tester analysis or $D^2$ analysis for maximum expression of heterosis. The level of heterosis expressed in the crossbreed population is determined by the interaction between genotype and prevailing environmental factors. Except some of the pre and post cocoon parameters, heterosis is invariably higher in single crosses compared to three-way and double crosses. However, during hot and humid season, when rearing of F1 bivoltine hybrid is unsuccessful at field level and indigenous races results in very low and poor quality yield, three-way and double crosses can play an important role as an intermediary technology. The objective of this article is to review briefly the concept and causes of heterosis, utilization of different forms of heterosis in silk production and its significance in silkworm, Bombyx mori breeding.

Inheritance Analysis of Giant Embryo Mutation Induced by T-DNA Insertion in Rice

  • Qin, Yang;Kim, Suk-Man;Park, Hee-Yeon;Sohn, Jae-Keun
    • Korean Journal of Breeding Science
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    • v.41 no.1
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    • pp.9-15
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    • 2009
  • Recently, giant embryonic rice and functional rice food are preferred by more consumers, which are attributed to the fact that the embryo has high concentrations of essential amino acids, fatty acids, and vitamins relative to other parts of rice grains. In this report, the heredity and stability of giant embryo mutations in successive generations were analyzed regarding a giant embryonic line, 'P47', induced by T-DNA insertion and a $F_2$ population from a cross between 'P47' and 'Junam'. The mutant lines with increases of 1.5, 1.7 and 1.8 times on embryo length, width and 100-embryo weight to those of the control showed stable inheritance across three generations. The continuous frequency distributions of embryo size in the $F_2$ population showed that the embryo size is a quantitative trait of polygene controlled. In addition, wide range of transgressive segregations of six traits affecting embryo size confirmed exchange of genetic materials and recombination between genes controlling embryo size. Five giant embryo mutant lines selected from the $F_2$ population will be used for artificial selection and improvement of giant embryonic varieties.

Biochemical and Molecular Analysis of OsPAP1: A Phosphate Starvation Induced Purple Acid Phosphatase Gene from Rice

  • Hur, Yeon Jae;Yi, Young Byung;Kim, Tae Ho;Kim, Doh Hoon
    • Korean Journal of Breeding Science
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    • v.42 no.5
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    • pp.455-462
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    • 2010
  • Purple acid phosphatase is important for phosphorus remobilization in plants, but its role in plant adaptation to low phosphorus availability is not known. The cDNA encoding O. sativa purple acid phosphatase (OsPAP1) has 1008 bp with an open reading frame of 335 amino acid residues. The amino acid sequence of OsPAP1 cDNA shows of 50-51% identity with other plant purple acid phosphatases. OsPAP1 was expressed in rice plants and in cell cultures in the absence of phosphate ($P_i$). The expression was organ-specific with the strongest expression in $P_i$-deprived roots. Functional expression of the OsPAP1 gene in the transgenic Arabidopsis line was confirmed by northern and western blot analysis. OsPAP1 overexpression lines had higher phosphatase activity than wild-type. Overexpression of OsPAP1 in Arabidopsis plants resulted in increased Pi accumulation under Pi sufficient condition. These results show that the OsPAP1 gene represents more efficient $P_i$ uptake and can be used to develop new transgenic dicotyledonous plants.

Characteristics of Endosperm Starch of the Rice Mutant Lines Induced by Sodium Azide

  • Shin, Young-Seop;Park, Chlul-Soo;Seo, Yong-Weon;Jeung, Ji-Ung
    • Korean Journal of Breeding Science
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    • v.41 no.2
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    • pp.84-91
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    • 2009
  • Rice consumption per capta, in South Korea, has been decreased dramatically, owing to the changes of living patterns. Because of not only the major energy source of Korean people but also major income source of Korean farmers, diversifying end-use-quality of rice has been demanded. To the context, 'Suweon 472', a high yielding and early mature japonica line and released as 'Namilbyeo' to framers in 2002, was treated with a chemical mutagen, Sodium Azide to find endosperm mutant types. A total of nine endosperm mutat lines, including five waxy, one dull, two floury, and one white core type, were identified from the 3,542 mutatagen treated lines. Amylose contents, iodine reaction, disintegration in alkali solution, gelatinization in urea solution and amylogram properties of those nine endosperm mutant lines were evaluated to address the possibility as new genetic materials for diversifying rice quality of Korean japonica cultivars. All embryo mutants were clearly differentiated from their wild type, 'Suweon 472', in terms of physic-chemical properties evaluated. The endosperm mutant lines would be very useful in expanding untiliztation of rice through opening new rice markets of processed foods from Korean japonica rice.