• Food Science of Animal Resources
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• v.42 no.1
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• pp.1-17
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• 2022

Lipids are one of the major macronutrients essential for adequate growth and maintenance of human health. Their structure is not only complex but also diverse, which makes systematic and holistic analyses challenging; consequently, little is known regarding the relationship between phenotype and mechanism of action. In recent years, rapid advancements have been made in the fields of lipidomics and bioinformatics. In comparison with traditional approaches, mass spectrometry-based lipidomics can rapidly identify as well as quantify >1,000 lipid species at the same time, facilitating comprehensive, robust analyses of lipids in tissues, cells, and body fluids. Accordingly, lipidomics is now being widely applied in various fields, particularly food and nutrition science. In this review, we discuss lipid classification, extraction techniques, and detection and analysis using lipidomics. We also cover how lipidomics is being used to assess food obtained from livestock and poultry. The information included herein should serve as a reference to determine how to characterize lipids in animal food samples, enhancing our understanding of the application of lipidomics in the field in animal husbandry.

• Journal of Veterinary Clinics
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• v.21 no.2
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• pp.115-121
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• 2004

The purpose of this study was to ascertain the breeding efficiency in Thoroughbred mare. A total of 106 mares were investigated for the status of follicle (462 cases), ovulation (179 cases) and pregnancy (346 cases). Of total examination, 46.8% was follicle measure to determine breeding time, and mating rate per cases examined was 39.9%. There was no correlation between reproductive results and size of follicles or endometrial edema or degrees of teasing alone. 143 cases were ovulated among 179 cases which were performed ovulation examination, and ovulation rate and fertilization rate per mating times were 79.9% and 39.0%, respectively. The use of hCG(human chorionic gonadotropin), to facilitate ovulation, presented to increase occurrence of double ovulations and twin fertilizations In conclusion, though more examination to estimate the optimal breeding time and higher mating rate was performed, fertilization rate per mating times was lower and then reproductive efficiency also became decreased. Therefore, it seemed that accurate examination of reproductive tracks, appropriate teasing programme and hCG administration before ovulation were of help to improve ovulation rate and fertilization rate.

• Asian-Australasian Journal of Animal Sciences
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• v.12 no.4
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• pp.511-514
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• 1999

Data pertaining to 1111 first lactation performance record of Karan Fries (Holstein-Friesian $\times$ Zebu) cows spread over a period of 21 years and sired by 72 bulls were used to examine the efficiency of sire indices for lactation milk production using auxiliary traits. First lactation length, first service period, first calving interval, first dry period and age at first calving were considered as auxiliary traits. The efficiency of this method was compared with simple daughter average index (D), contemporary comparison method (CC), least-square method (LSQ), simplified regressed least-squares method (SRLS) and best linear unbiased prediction (BLUP) for lactation milk production. The relative efficiency of sire evaluation methods using one auxiliary trait was lower (24.2-32.8%) in comparison to CC method, the most efficient method observed in this study. Use of two auxiliary traits at a time did not further improve the efficiency. The auxiliary sire indices discriminate better among bulls as the range of breeding values were higher in these methods in comparison to conventional sire evaluation methods. The rank correlation between breeding values estimated using auxiliary traits were high (0.77-0.78) with CC method. The rank correlation among auxiliary sire indices ranged from 0.98 to 0.99, indicating similar ranking of sire for breeding values of milk production in all the auxiliary sire indices.

• Asian-Australasian Journal of Animal Sciences
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• v.20 no.5
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• pp.661-668
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• 2007

Data on 1,037 Nili-Ravi buffaloes from four institutional herds were used to study lifetime milk yield, herd life, productive life and breeding efficiency. A general linear model was used to study the environmental effects while an animal model having herd, year of birth and age at first calving (as covariate) along with random animal effect was used to estimate breeding values. The lifetime milk yield, herd life, productive life and breeding efficiency averaged $7,723{\pm}164$ kg, $3,990{\pm}41$ days, $1,061{\pm}19$ days and 64 percent, respectively. All the traits were significantly (p<0.01) affected by the year of birth and herd of calving, while the herd life was also affected (p<0.01) by the age at first calving. The heritabilities for lifetime milk yield, herd life, productive life and breeding efficiency were $0.093{\pm}0.056$, $0.001{\pm}0.055$, $0.144{\pm}0.079$ and 0.001, respectively. The definition for productive life, where each lactation gets credit upto 10 months had slightly better heritability and may be preferred over the definition where no limit is placed on lactation length. The genetic correlation between productive life and lifetime milk yield was low but high between productive life and herd life. The selection for productive life will increase herd life while lifetime milk yield will also improve. The overall phenotypic trend during the period under the study was negative for lifetime milk yield (-280 kg/year), herd life (-93 days), productive life (-42 days/year) and breeding efficiency (-0.36 percent/year), whereas the genetic trend was positive for lifetime milk yield (+15 kg/year) and productive life (+4 days/year).

• Asian-Australasian Journal of Animal Sciences
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• v.15 no.4
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• pp.466-469
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• 2002

The data on 512 Hariana cows, progeny of 20 sires calved during period from 1974 to 1993 maintained at Government Livestock Farm, Hisar were considered for the estimation of genetic parameters. The means for first lactation milk yield (FLY), wet average (WA), first lactation peak yield (FPY), first lactation milk yield per day of first calving interval (MCI) and first lactation milk yield per day of age at second calving (MSC) were 1,141.58 kg, 4.19 kg/day, 6.24 kg/day, 2.38 kg/day and 0.601 kg/day, respectively. The effect of period of calving was significant (p<0.05) on WA, FPY and MCI while the effect of season of calving was significant only on WA. Monsoon calvers excelled in performance for all the production efficiency traits. The effect of age at first calving (linear) was significant on all the traits except on MCI. Estimates of heritabilty for all the traits were moderate and ranged from 0.255 to 0.333 except for WA (0.161). All the genetic and phenotypic correlations among different production efficiency traits were high and positive. It may be inferred that selection on the basis of peak yield will be more effective as the trait is expressed early in life and had reasonably moderate estimate of heritability.

• Food Science of Animal Resources
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• v.44 no.1
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• pp.165-177
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• 2024

Volatile compounds (VOCs) are an important factor affecting meat quality. However, the characteristic VOCs in different parts of donkey meat remain unknown. Accordingly, this study represents a preliminary investigation of VOCs to differentiate between different cuts of donkey meat by using headspace-gas chromatography-ion mobility spectrometry (HS-GC-IMS) combined with chemometrics analysis. The results showed that the 31 VOCs identified in donkey meat, ketones, alcohols, aldehydes, and esters were the predominant categories. A total of 10 VOCs with relative odor activity values ≥1 were found to be characteristic of donkey meat, including pentanone, hexanal, nonanal, octanal, and 3-methylbutanal. The VOC profiles in different parts of donkey meat were well differentiated using three- and two-dimensional fingerprint maps. Nine differential VOCs that represent potential markers to discriminate different parts of donkey meat were identified by chemometrics analysis. These include 2-butanone, 2-pentanone, and 2-heptanone. Thus, the VOC profiles in donkey meat and specific VOCs in different parts of donkey meat were revealed by HS-GC-IMS combined with chemometrics, whcih provided a basis and method of investigating the characteristic VOCs and quality control of donkey meat.

• Journal of Veterinary Clinics
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• v.20 no.1
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• pp.121-130
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• 2003

The purpose of this study was to investigate the effect of breeding conditions on reproductive efficiency of thoroughbred broodmares by ultrasonography. The mean age and breeding career of 120 mares used in this study were 11 years old and 6 years, respectively. The average pregnancy rate and embryonic loss rate were 87.5%, 11.8%. The groups that were 8-10 years old, 4-6 years, maiden and mated on May showed the highest pregnancy rate. While mares mated on 1st estrus post partus had the highest ovulation rate but showed the highest embryonic loss rate and he lowest pregnancy rate. And broodmares that had more intrauterine foreign bodies such as cyst and fluid with age and breeding career had represented lower pregnancy rate and higher embryonic loss rate than those having normal uterine condition. These results suggest that the breeding condition and uterine environment of mares had a significant influence on reproductive efficiency.

• Journal of Animal Science and Technology
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• v.64 no.2
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• pp.312-329
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• 2022

Feed cost is the main factor affecting the economic benefits of pig industry. Improving the feed efficiency (FE) can reduce the feed cost and improve the economic benefits of pig breeding enterprises. Liver is a complex metabolic organ which affects the distribution of nutrients and regulates the efficiency of energy conversion from nutrients to muscle or fat, thereby affecting feed efficiency. MicroRNAs (miRNAs) are small non-coding RNAs that can regulate feed efficiency through the modulation of gene expression at the post-transcriptional level. In this study, we analyzed miRNA profiling of liver tissues in High-FE and Low-FE pigs for the purpose of identifying key miRNAs related to feed efficiency. A total 212~221 annotated porcine miRNAs and 136~281 novel miRNAs were identified in the pig liver. Among them, 188 annotated miRNAs were co-expressed in High-FE and Low-FE pigs. The 14 miRNAs were significantly differentially expressed (DE) in the livers of high-FE pigs and low-FE pigs, of which 5 were downregulated and 9 were upregulated. Kyoto Encyclopedia of Genes and Genomes analysis of liver DE miRNAs in high-FE pigs and low-FE pigs indicated that the target genes of DE miRNAs were significantly enriched in insulin signaling pathway, Gonadotropin-releasing hormone signaling pathway, and mammalian target of rapamycin signaling pathway. To verify the reliability of sequencing results, 5 DE miRNAs were randomly selected for quantitative reverse transcription-polymerase chain reaction (qRT-PCR). The qRT-PCR results of miRNAs were confirmed to be consistent with sequencing data. DE miRNA data indicated that liver-specific miRNAs synergistically acted with mRNAs to improve feed efficiency. The liver miRNAs expression analysis revealed the metabolic pathways by which the liver miRNAs regulate pig feed efficiency.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.43 no.5
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• pp.457-461
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• 2010

To estimate the effect of selective breeding on the improvement of growth in olive flounder Paralichthys olivaceus, we compared the growth of a strain selected for rapid growth to that of an unselected strain from a commercial hatchery. The fish strains were fed with either moist pellets (MP) (dry matter, 59.8% crude protein; 14.1% lipid) or extruded pellets (EP) (dry matter, 50.4% crude protein; 13.8% lipid) for 190 days and were reared under similar conditions. The mortality rates were less than 2% and were not significantly different among the experimental groups. The growth rate of the selected fish was significantly greater than that of the unselected fish regardless of the diet type, and both strains fed MP grew significantly faster than those fed EP. The selected fish consumed more feed than the unselected fish. However, there was no significant difference in the feed efficiency between the selected and unselected fish. These results demonstrate that the selected fish exhibited superior growth rates, and that this was associated with a greater intake of food. Thus, selective breeding may be useful for improving the growth of commercial olive flounder.

• Journal of Plant Biotechnology
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• v.42 no.3
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• pp.154-160
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• 2015

The plant breeding technology was developed with genetic engineering. Many researchers and breeders have turned from traditional breeding to molecular breeding. Genetically modified organisms (GMO) were developed via molecular breeding technology. Currently, molecular breeding technologies facilitate efficient plant breeding without introducing foreign genes, in virtue by of gene editing technology. Gene targeting (GT) via homologous recombination (HR) is one of the best gene editing methods available to modify specific DNA sequences in genomes. GT utilizes DNA repair pathways. Thus, DNA repair systems are controlled to enhance HR processing. Engineered sequence specific endonucleases were applied to improve GT efficiency. Engineered sequence specific endonucleases like the zinc finger nuclease (ZFN), TAL effector nuclease (TALEN), and CRISPR-Cas9 create DNA double-strand breaks (DSB) that can stimulate HR at a target site. RecQl4, Exo1 and Rad51 are effectors that enhance DSB repair via the HR pathway. This review focuses on recent developments in engineered sequence specific endonucleases and ways to improve the efficiency of GT via HR effectors in plants.