• 전자공학회논문지SC
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• 제39권1호
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• pp.7-15
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• 2002

원거리에 분산되어 있는 다수의 양식시설을 마이크로프로세서를 이용하여 원격으로 환경상태를 감시하고 제어하기 위한 알고리즘을 구현하였다. 각각의 시설에서 검출한 환경데이터를 하나의 프로세서에 수집한 후, 전화선을 이용하여 FSK 방식으로 원거리의 관리자에게 전송하고 데이터를 수신하여 표시기에 나타내므로써 시설들의 환경상태를 알 수 있다. 또한 관리자는 시설의 환경을 조절하는 제어기의 작동데이터를 설정하여 전송하면 해당시설로 전달되어 원격으로 환경을 제어할 수 있다. 다수의 시설에서 검출된 환경데이터를 최소의 회선을 사용하여 한곳으로 취득하기 위한 방법으로 멀티-프로세싱 기법을 적용하였고, 취득한 데이터와 제어용 데이터의 상호교환을 위한 알고리즘을 연구하였다. 먼저 프로세서 상호간의 통신시？스를 설계하여 데이터의 교환에 대한 통신알고리즘을 실험적으로 구현하였으며, 전화회선의 통과주파수 대역에서 전송특성의 검토로 설계한 FSK 변복조기의 성능을 분석하였다. 또한 대량의 시설을 관리하기 위하여 PC를 활용한 DB구축에 대한 알고리즘도 제시하였다. 본 연구의 목적은 최소의 비용으로 쉽게 사용할 수 있는 방법의 구현에 있으며, 시설의 관리에 소요되는 노동력의 감소와 환경상태의 악화에 의한 만약의 사태를 예방할 수 있는 경보를 제공하는 것으로 양식산업을 보다 과학적이고 기술적인 방향으로 개선하는데 있다.

• BMB Reports
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• 제43권7호
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• pp.491-498
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• 2010

Chemerin is a novel adipokine which is abundant in adipose tissue to promote adipocyte differentiation and with significant relativity to BMI and insulin sensitivity. We report here the molecular characterization of porcine chemerin and its receptors ChemR23 and GPR1, as well as their transcriptional regulation during lipogenesis. Chemerin was mainly expressed in liver, intestine, kidney and adipose tissue, consistent with the expression pattern of GPR1, but not ChemR23, which was predominantly present in spleen and temperately in adipose tissue. We further investigated the lipogenesis-related transcriptional activation of $PPAR{\gamma}$ and KLF15 on chemerin and its receptors. The data showed that KLF15, but not $PPAR{\gamma}$, can up-regulate the mRNA level of chemerin, ChemR23 and GPR1, which was consistent with the results of luciferase assay that confirmed the effect of KLF15 on ChemR23 promoter. Taken together, our data provide basic molecular information for the further investigation on the function of chemerin in lipogenesis.

• Asian-Australasian Journal of Animal Sciences
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• 제33권11호
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• pp.1725-1731
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• 2020

Objective: An initial RNA-Sequencing study revealed that UDP-galactose-4-epimerase (GALE) was one of the most promising candidates for milk protein concentration in Chinese Holstein cattle. This enzyme catalyzes the interconversion of UDP-galactose and UDP-glucose, an important step in galactose catabolism. To further validate the genetic effect of GALE on milk protein traits, genetic variations were identified, and genotypes-phenotypes associations were performed. Methods: The entire coding region and the 5'-regulatory region (5'-UTR) of GALE were re-sequenced using pooled DNA of 17 unrelated sires. Association studies for five milk production traits were performed using a mixed linear animal model with a population encompassing 1,027 Chinese Holstein cows. Results: A total of three variants in GALE were identified, including two novel variants (g.2114 A>G and g.2037 G>A) in the 5'-UTR and one previously reported variant (g.3836 G>C) in an intron. All three single nucleotide polymorphisms (SNPs) were associated with milk yield (p<0.0001), fat yield (p = 0.0006 to <0.0001), protein yield (p = 0.0232 to <0.0001) and protein percentage (p<0.0001), while no significant associations were detected between the SNPs and fat percentage. A strong linkage disequilibrium (D' = 0.96 to 1.00) was observed among all three SNPs, and a 5 Kb haplotype block involving three main haplotypes with GAG, AGC, and AGG was formed. The results of haplotype association analyses were consistent with the results of single locus association analysis (p<0.0001). The phenotypic variance ratio above 3.00% was observed for milk protein yield that was explained by SNP-g.3836G >C. Conclusion: Overall, our findings provided new insights into the polymorphic variations in bovine GALE gene and their associations with milk protein concentration. The data indicate their potential uses for marker-assisted breeding or genetic selection schemes.

• 한국발생생물학회지:발생과생식
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• 제24권3호
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• pp.187-196
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• 2020

The caspase10 encodes an initiating caspase that plays an important role in the maintaining the cellular homeostasis by regulating the steps involved in the immune response and cell death. We investigated the expression of caspase10 during the different developmental stages and in olive flounder tissues. Caspase10 increased in the late stage of the formation of immune tissue, and high expression was observed in the gills, kidney, skin, and spleen. The current study analyzed the expressional changes of caspase10 in olive flounder infected with viral hemorrhagic septicemia virus (VHSV). One of the major causes of mass mortality, VHSV infection in olive flounder attributes to significant expression of caspase10 in the gills, spleen, skin, and kidneys. The results indicate a close association of caspase10 expression with the immune response to VHSV infection in olive flounder. The observations could form the basis data for exploration of other fish immune system.

• 한국수산과학회지
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• 제54권6호
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• pp.974-981
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• 2021

The aim of this study was to evaluate genetic parameters of growth traits for improving selective breeding in olive flounder Paralichthys olivaceus produced in April 2019. The total length and body weight at 11-, 18-, and 22-months-old were measured for 7,479, 2,831 and 1,904 individuals, respectively. Since 2004, we have been conducting a selective breeding program to improve growth traits in the olive flounder. Data were analyzed using the restricted maximum likelihood method using the multiple traits animal model. The effect of sex and production period showed significant differences in all traits (P<0.05). The heritability of all traits was 0.428-0.520, which is relatively high by measurement month. Therefore, it is considered that individual selection will be more advantageous than family selection. However, to maintain an appropriate degree of inbreeding and genetic diversity for future generations, it is necessary to consider family selection adequately. Results of the correlation analysis between the same traits according to the measurement period indicated that considering production costs such as feed cost, selection at 18-months-old will be advantageous. Olive flounder is a major aquaculture species in Korea, and continuous selective breeding research is essential to improve productivity.

• Asian-Australasian Journal of Animal Sciences
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• 제29권6호
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• pp.775-781
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• 2016

A single trait linear mixed random regression test-day model was applied for the first time for analyzing the first lactation monthly test-day milk yield records in Karan Fries cattle. The test-day milk yield data was modeled using a random regression model (RRM) considering different order of Legendre polynomial for the additive genetic effect (4th order) and the permanent environmental effect (5th order). Data pertaining to 1,583 lactation records spread over a period of 30 years were recorded and analyzed in the study. The variance component, heritability and genetic correlations among test-day milk yields were estimated using RRM. RRM heritability estimates of test-day milk yield varied from 0.11 to 0.22 in different test-day records. The estimates of genetic correlations between different test-day milk yields ranged 0.01 (test-day 1 [TD-1] and TD-11) to 0.99 (TD-4 and TD-5). The magnitudes of genetic correlations between test-day milk yields decreased as the interval between test-days increased and adjacent test-day had higher correlations. Additive genetic and permanent environment variances were higher for test-day milk yields at both ends of lactation. The residual variance was observed to be lower than the permanent environment variance for all the test-day milk yields.

• Asian-Australasian Journal of Animal Sciences
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• 제24권2호
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• pp.162-172
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• 2011

In this paper, simulation was used to determine accuracies of genomic breeding values for polygenic traits associated with many thousands of markers obtained from high density genome scans. The statistical approach was based upon stochastically simulating a pedigree with a specified base population and a specified set of population parameters including the effective and noneffective marker distances and generation time. For this population, marker and quantitative trait locus (QTL) genotypes were generated using either a single linkage group or multiple linkage group model. Single nucleotide polymorphism (SNP) was simulated for an entire bovine genome (except for the sex chromosome, n = 29) including linkage and recombination. Individuals drawn from the simulated population with specified marker and QTL genotypes were randomly mated to establish appropriate levels of linkage disequilibrium for ten generations. Phenotype and genomic SNP data sets were obtained from individuals starting after two generations. Genetic prediction was accomplished by statistically modeling the genomic relationship matrix and standard BLUP methods. The effect of the number of linkage groups was also investigated to determine its influence on the accuracy of breeding values for genomic selection. When using high density scan data (0.08 cM marker distance), accuracies of breeding values on juveniles were obtained of 0.60 and 0.82, for a low heritable trait (0.10) and high heritable trait (0.50), respectively, in the single linkage group model. Estimates of 0.38 and 0.60 were obtained for the same cases in the multiple linkage group models. Unexpectedly, use of BLUP regression methods across many chromosomes was found to give rise to reduced accuracy in breeding value determination. The reasons for this remain a target for further research, but the role of Mendelian sampling may play a fundamental role in producing this effect.

• Molecules and Cells
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• 제38권4호
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• pp.304-311
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• 2015

Most follicles in the mammalian ovary undergo atresia. Granulosa cell apoptosis is a hallmark of follicle atresia. Our previous study using a microRNA (miRNA) microarray showed that the let-7 microRNA family was differentially expressed during follicular atresia. However, whether the let-7 miRNA family members are related to porcine (Sus scrofa) ovary follicular apoptosis is unclear. In the current study, real-time quantitative polymerase chain reaction showed that the expression levels of let-7 family members in follicles and granulosa cells were similar to our microarray data, in which miRNAs let-7a, let-7b, let-7c, and let-7i were significantly decreased in early atretic and progressively atretic porcine ovary follicles compared with healthy follicles, while let-7g was highly expressed during follicle atresia. Furthermore, flow cytometric analysis and Hoechst33342 staining demonstrated that let-7g increased the apoptotic rate of cultured granulosa cells. In addition, let-7 target genes were predicted and annotated by TargetScan, PicTar, gene ontology and Kyoto encyclopedia of genes and genomes pathways. Our data provide new insight into the association between the let-7 miRNA family in granulosa cell programmed death.

• 한국패류학회지
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• 제25권3호
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• pp.197-201
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• 2009

본 연구에서는 높은 변이를 나타내는 6개의 Microsatellite DNA 유전표식을 이용하여 독도지역에 서식하고 있는 자연산 전복집단의 유전적 다양성 및 집단구조를 파악하여 동 서 남해의 6개 지역집단과 비교 분석하였다. 그 결과 독도지역의 유전적 다양성은 6개 지역집단보다 높게 나타났으며 유전적 거리에 의한 유연관계 분석 결과에 있어서도 이들 집단과는 독립된 집단으로 나타났다. 이러한 결과는 6개 지역집단에 있어 방류전복의 높은 혼획비율에 의한 자연산 집단의 유전적 다양성이 축소되어진 결과라고 생각되어진다. 따라서 독도집단의 높은 유전적 다양성을 유지하고 보존하기 위해서는 이 지역의 유전적 다양성을 고려한 체계화된 전복종묘의 방류가 이루어져야하며, 방류를 한 이후에도 지속적인 유전학적 모니터링이 필요하다고 생각되어진다.

• Asian-Australasian Journal of Animal Sciences
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• 제19권7호
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• pp.927-930
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• 2006

This study was conducted to investigate potential effects of previously identified QTL regions on carcass traits in Hanwoo. The data analyzed in this study was collected from 326 steers of 67 proven sire. Thirteen micorsatellite markers spanning QTL regions on bovine chromosomes 1 and 14 were genotyped in 326 steers. The following breeding values were analyzed for QTL effects. Cold carcass weight breeding value (CCWBV), longissimus muscle area breeding value (LMABV), marbling score breeding value (MSBV) and backfat thickness breeding value (BFTBV). Chi-square tests were performed to compare frequencies of individual allele between high and low breeding value groups. Significant differences of allele frequencies in BMS711, MCM130, BMS4049, and BMS2263 were found. And also, in RM180, BL1029, BM4305, and BMS2055 there were significant differencies of allele frequencies. These results showed a potential application for investigation of putative QTL locations.