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http://dx.doi.org/10.5483/BMBRep.2010.43.7.491

Cloning of porcine chemerin, ChemR23 and GPR1 and their involvement in regulation of lipogenesis  

Huang, Jianfeng (Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Life Science and Technology, Huazhong Agricultural University)
Zhang, Jian (Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Life Science and Technology, Huazhong Agricultural University)
Lei, Ting (Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Life Science and Technology, Huazhong Agricultural University)
Chen, Xiaodong (Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Life Science and Technology, Huazhong Agricultural University)
Zhang, Yan (Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Life Science and Technology, Huazhong Agricultural University)
Zhou, Lulu (Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Life Science and Technology, Huazhong Agricultural University)
Yu, An (Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Life Science and Technology, Huazhong Agricultural University)
Chen, Zhilong (Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Life Science and Technology, Huazhong Agricultural University)
Zhou, Ronghua (Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Life Science and Technology, Huazhong Agricultural University)
Yang, Zaiqing (Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Life Science and Technology, Huazhong Agricultural University)
Publication Information
BMB Reports / v.43, no.7, 2010 , pp. 491-498 More about this Journal
Abstract
Chemerin is a novel adipokine which is abundant in adipose tissue to promote adipocyte differentiation and with significant relativity to BMI and insulin sensitivity. We report here the molecular characterization of porcine chemerin and its receptors ChemR23 and GPR1, as well as their transcriptional regulation during lipogenesis. Chemerin was mainly expressed in liver, intestine, kidney and adipose tissue, consistent with the expression pattern of GPR1, but not ChemR23, which was predominantly present in spleen and temperately in adipose tissue. We further investigated the lipogenesis-related transcriptional activation of $PPAR{\gamma}$ and KLF15 on chemerin and its receptors. The data showed that KLF15, but not $PPAR{\gamma}$, can up-regulate the mRNA level of chemerin, ChemR23 and GPR1, which was consistent with the results of luciferase assay that confirmed the effect of KLF15 on ChemR23 promoter. Taken together, our data provide basic molecular information for the further investigation on the function of chemerin in lipogenesis.
Keywords
Chemerin; Chemerin receptors; Lipogenesis; Porcine; Transcriptional regulation;
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1 Muruganandan, S., Roman, A. and Sinal, C. J. (2009) Role of Chemerin/CMKLR1 Signaling in Adipogenesis and Osteoblastogenesis of Bone Marrow Stem Cells. J. Bone Miner. Res. 25, 222-234.
2 Yerle, M., Echard, G., Robic, A., Mairal, A., Dubut- Fontana, C., Riquet, J., Pinton, P., Milan, D., Lahbib- Mansais, Y. and Gellin, J. (1996) A somatic cell hybrid panel for pig regional gene mapping characterized by molecular cytogenetics. Cytogenet. Cell Genet. 73, 194-202.   DOI
3 Ramirez-Zacarias, J. L., Castro-Munozledo, F. and Kuri- Harcuch, W. (1992) Quantitation of adipose conversion and triglycerides by staining intracytoplasmic lipids with Oil red O. Histochemistry 97, 493-497.   DOI
4 Zabel, B. A., Nakae, S., Zuniga, L., Kim, J. Y., Ohyama, T., Alt, C., Pan, J., Suto, H., Soler, D., Allen, S. J., Handel, T. M., Song, C. H., Galli, S. J. and Butcher, E. C. (2008) Mast cell-expressed orphan receptor CCRL2 binds chemerin and is required for optimal induction of IgE-mediated passive cutaneous anaphylaxis. J. Exp. Med. 205, 2207-2220.   DOI   ScienceOn
5 Barnea, G., Strapps, W., Herrada, G., Berman, Y., Ong, J., Kloss, B., Axel, R. and Lee, K. J. (2008) The genetic design of signaling cascades to record receptor activation. Proc. Natl. Acad. Sci. U.S.A. 105, 64-69.   DOI   ScienceOn
6 Zabel, B. A., Zuniga, L., Ohyama, T., Allen, S. J., Cichy, J., Handel, T. M. and Butcher, E. C. (2006) Chemoattractants, extracellular proteases, and the integrated host defense response. Exp. Hematol. 34, 1021-1032.   DOI   ScienceOn
7 Pease, J. E. and Williams, T. J. (2006) The attraction of chemokines as a target for specific anti-inflammatory therapy. Br. J. Pharmacol. 147(Suppl 1), S212-221.   DOI   ScienceOn
8 Martensson, U. E., Owman, C. and Olde, B. (2004) Genomic organization and promoter analysis of the gene encoding the mouse chemoattractant-like receptor, CMKLR1. Gene 328, 167-176.   DOI   ScienceOn
9 Otteson, D. C., Lai, H., Liu, Y. and Zack, D. J. (2005) Zinc-finger domains of the transcriptional repressor KLF15 bind multiple sites in rhodopsin and IRBP promoters including the CRS-1 and G-rich repressor elements. BMC Mol. Biol. 6, 15.   DOI
10 Wittamer, V., Gregoire, F., Robberecht, P., Vassart, G., Communi, D. and Parmentier, M. (2004) The C-terminal nonapeptide of mature chemerin activates the chemerin receptor with low nanomolar potency. J. Biol. Chem. 279, 9956-9962.   DOI   ScienceOn
11 Weigert, J., Neumeier, M., Wanninger, J., Filarsky, M., Bauer, S., Wiest, R., Farkas, S., Scherer, M. N., Schaffler, A., Aslanidis, C., Scholmerich, J. and Buechler, C. (2009) Systemic chemerin is related to inflammation rather than obesity in type 2 diabetes. Clin. Endocrinol. (Oxf). 72, 342-348.   DOI   ScienceOn
12 Mori, T., Sakaue, H., Iguchi, H., Gomi, H., Okada, Y., Takashima, Y., Nakamura, K., Nakamura, T., Yamauchi, T., Kubota, N., Kadowaki, T., Matsuki, Y., Ogawa, W., Hiramatsu, R. and Kasuga, M. (2005) Role of Kruppel-like factor 15 (KLF15) in transcriptional regulation of adipogenesis. J. Biol. Chem. 280, 12867-12875.   DOI   ScienceOn
13 Bozaoglu, K., Bolton, K., McMillan, J., Zimmet, P., Jowett, J., Collier, G., Walder, K. and Segal, D. (2007) Chemerin is a novel adipokine associated with obesity and metabolic syndrome. Endocrinology 148, 4687-4694.   DOI   ScienceOn
14 Lehrke, M., Becker, A., Greif, M., Stark, R., Laubender, R. P., von Ziegler, F., Lebherz, C., Tittus, J., Reiser, M., Becker, C., Goke, B., Leber, A. W., Parhofer, K. G. and Broedl, U. C. (2009) Chemerin is associated with markers of inflammation and components of the metabolic syndrome but does not predict coronary atherosclerosis. Eur. J. Endocrinol. 161, 339-344.   DOI   ScienceOn
15 Roh, S. G., Song, S. H., Choi, K. C., Katoh, K., Wittamer, V., Parmentier, M. and Sasaki, S. (2007) Chemerin--a new adipokine that modulates adipogenesis via its own receptor. Biochem. Biophys. Res. Commun. 362, 1013-1018.   DOI   ScienceOn
16 Goralski, K. B., McCarthy, T. C., Hanniman, E. A., Zabel, B. A., Butcher, E. C., Parlee, S. D., Muruganandan, S. and Sinal, C. J. (2007) Chemerin, a novel adipokine that regulates adipogenesis and adipocyte metabolism. J. Biol. Chem. 282, 28175-28188.   DOI   ScienceOn
17 Takahashi, M., Takahashi, Y., Takahashi, K., Zolotaryov, F. N., Hong, K. S., Kitazawa, R., Iida, K., Okimura, Y., Kaji, H., Kitazawa, S., Kasuga, M. and Chihara, K. (2008) Chemerin enhances insulin signaling and potentiates insulin- stimulated glucose uptake in 3T3-L1 adipocytes. FEBS Lett. 582, 573-578.   DOI   ScienceOn
18 Nagpal, S., Patel, S., Jacobe, H., DiSepio, D., Ghosn, C., Malhotra, M., Teng, M., Duvic, M. and Chandraratna, R. A. (1997) Tazarotene-induced gene 2 (TIG2), a novel retinoid- responsive gene in skin. J. Invest. Dermatol. 109, 91-95.   DOI   ScienceOn
19 Sell, H., Laurencikiene, J., Taube, A., Eckardt K, Cramer, A., Horrighs, A., Arner, P. and Eckel, J. (2009) Chemerin is a novel adipocyte-derived factor inducing insulin resistance in primary human skeletal muscle cells. Diabetes 58, 2731-2740.   DOI   ScienceOn
20 Trujillo, M. E. and Scherer, P. E. (2006) Adipose tissue-derived factors: impact on health and disease. Endocr. Rev. 27, 762-778.   DOI   ScienceOn
21 Parolini, S., Santoro, A., Marcenaro, E., Luini, W., Massardi, L., Facchetti, F., Communi, D., Parmentier, M., Majorana, A., Sironi, M., Tabellini, G., Moretta, A. and Sozzani, S. (2007) The role of chemerin in the colocalization of NK and dendritic cell subsets into inflamed tissues. Blood 109, 3625-3632.   DOI   ScienceOn
22 Wittamer, V., Franssen, J. D., Vulcano, M., Mirjolet, J. F., Le Poul, E., Migeotte, I., Brezillon, S., Tyldesley, R., Blanpain, C., Detheux, M., Mantovani, A., Sozzani, S., Vassart, G., Parmentier, M. and Communi, D. (2003) Specific recruitment of antigen-presenting cells by chemerin, a novel processed ligand from human inflammatory fluids. J. Exp. Med. 198, 977-985.   DOI   ScienceOn
23 Wittamer, V., Bondue, B., Guillabert, A., Vassart, G., Parmentier, M. and Communi, D. (2005) Neutrophil-mediated maturation of chemerin: a link between innate and adaptive immunity. J. Immunol. 175, 487-493.   DOI
24 Zabel, B. A., Allen, S. J., Kulig, P., Allen, J. A., Cichy, J., Handel, T. M. and Butcher, E. C. (2005) Chemerin activation by serine proteases of the coagulation, fibrinolytic, and inflammatory cascades. J. Biol. Chem. 280, 34661-34666.   DOI   ScienceOn
25 Guillabert, A., Wittamer, V., Bondue, B., Godot, V., Imbault, V., Parmentier, M. and Communi, D. (2008) Role of neutrophil proteinase 3 and mast cell chymase in chemerin proteolytic regulation. J. Leukoc. Biol. 84, 1530-1538.   DOI   ScienceOn
26 Hotamisligil, G. S. (2006) Inflammation and metabolic disorders. Nature 444, 860-867.   DOI   ScienceOn
27 Cash, J. L., Hart, R., Russ, A., Dixon, J. P., Colledge, W. H., Doran, J., Hendrick, A. G., Carlton, M. B. and Greaves, D. R. (2008) Synthetic chemerin-derived peptides suppress inflammation through ChemR23. J. Exp. Med. 205, 767-775.   DOI   ScienceOn