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A Systematic Classification of Korean Fagaceae by the Pollen (화분(花粉)에 의한 한국산(韓國產) 참나무과(科) 계통분류(系統分類))

  • Park, Seung Young
    • Journal of Korean Society of Forest Science
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    • v.80 no.2
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    • pp.151-161
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    • 1991
  • I tried to specify the taxa of Fagaceae in Korea by the character of their pollen grains. The light microscope(LM) and the Scanning electron microscope(SEM) were used to examine the pollen grains of 19 taxa, 5 genera. The result are as follows. 1. The pollen of Fagaceae in Korea could be grouped into four types and 4 subtypes. 1) Fagus type 2) Castanea type 3) Lithocarpus, Castanopsis type 4) Quercus type (1) Cyclobalanopsis subtype (2) Prinus subtype (3) Dentatae subtype (4) Cerris subtype. 2. The morphology of the granula on the pollen of Quercus was closely related to the differantiation of the shape of the cup scales. 1) The uniformity of branching granula on the pollen grain surface corresponds to the morphological features of the concentric arrangement of cup scales. 2) The morphological features of the pollen grain surface intermingled with large or small granula, simple-granula and tuber granula which have small points of circular prominence, corresponded to those of short cup scales. 3) The morphological features of the polllen grain surface intermingled with large or small granula, simple-granula and tuber granula with an apex of amoeba type corresponded to those of Q. dentata Thunb, with thin, fine and long cup scales. 4) The morphological features of the pollen grain surface intermingled with large of small granula of with only simple-granula, corresponded to those of Q. acutissima carr. with thick, fine and long cup scales. 3. The result of cluster analysis by coding the sculpture pattern of the pollen grain surface, the existence and nonexistence of surface perforate, the grain size and granula type were coincident with the system of classification of plants and showed an intimated relationship even under th level of species.

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A STUDY ON THE DISTRIBUTION OF CALCITONIN GENE-RELATED PEPTIDE CONTAINING NERVE FIBERS IN RAT PULP FOLLOWING DENTINAL INJURY (상아질 손상 후 흰쥐 대구치 치수의 calcitonin gene-related peptide(CGRP) 함유 신경섬유 분포에 관한 연구)

  • Moon, Joo-Hoon;Park, Sang-Jin;Min, Byung-Soon;Choi, Ho-Young;Cho, Gi-Woon
    • Restorative Dentistry and Endodontics
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    • v.24 no.1
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    • pp.100-115
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    • 1999
  • The purpose of this study was to investigate the distribution of calcitonin gene-related peptide containing nerve fibers in rat pulp after dentinl injury by means of immunohistochemistry and confocal laser scanning microscope. The Spague-Dawley rats weighing about 250-300gm were used. The animals were devided into normal control and experimental groups. Experimental animals were sacrified 1, 2, 4, 7, 10, 21days after dentinal injury (dentin cutting, and then acid etching with 35% phosphoric acid) on the maxillary molar teeth. The maxillary teeth and alveolar bone were removed and immersed in the 4% paraformaldehyde in 0.1M phosphate buffer (pH 7.4), then were decalcified with 15% formic acid for 10 days. Serial frozen $50{\mu}m$ thick sections were cut on a cryostat. The rabbit CGRP antibody was used as a primary antibody with a dilution of 1:2000 in 0.01M PB. The sections were incubated for 48 hours at $4^{\circ}C$, and placed into biotinylated antirabbit Ig G as a secondary anti body with dilution of 1:200 in 0.01M PB and incubated in ABC(avidin-biotin complex). The peroxidase reaction was visualized by incubating the sections in 0.05% 3,3 diaminobenzidine tetrahydrochloride containing 0.02% $H_2O_2$. For the confocal laser scanning microscopic examination, Primary antibody reaction was same as immunoperoxidase stainning, but fluorescein isothiocyanate(FITC)-conjugate antirabbit IgG as a secondary antibody was used. The confocal laser scanning microscope was used for the examination. A series of images of optical sections was collected with a 20x objective at $3{\mu}m$ intervals throughout the depth of specimen. FITC fluerescence was registrated through a 488nm and 568nm excitation filter, and images were saved on optical disk. The stereoscopic images and three dimentionnal images were reconstructed by computer software, and then were analyzed. The results were as follows : 1. In normal control group, CGRP containing nerve fibers were coursed through the root with very little branching, and then formed a dense network of terminals in coronal pulp. 2. A slight increase in CGRP containing nerve fibers at 1 and 2day postinjury was noted subjacent to the injury site. In the 4day group, there were an extensive increase in the number of reactive fibers, followed by a partial return toward normal levels at 7~10 day postinjury, and return by 21days. 3. The sprouting of the CGRP containing nerve fibers was evident within 2day after dentinal injury, and by 4days there was a maximal increased, but was decreased at 7days and returned to normal 10~21 day postinjury. 4. In confocal laser scanning microscopic exammination, the distinct distribution pattern and sprouting reaction of CGRP containing nerve fibers were observed in stereoscopic images and three dimentional images. These results suggest that CGRP containing nerve fiber can be important role in the response to dental injury and pain regulation.

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Differentiation between Morgagni Hernia and Pleuropericardial Fat with Using CT Findings (CT 소견을 이용한 Morgagni 탈장과 심막주위지방의 감별)

  • Kim Sung-Jin;Cho Beum-Sang;Lee Seung-Young;Bae Il-Hun;Han Ki-Seok;Lee Ki-Man;Hong Jong-Myeon
    • Journal of Chest Surgery
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    • v.39 no.8 s.265
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    • pp.573-578
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    • 2006
  • Background: Generally hernia is diagnosed with simple chest or gastrointestinal x-ray. Sometimes CT or MRI can give lots of information for the diagnosis. However, there was no study for the differentiation with using CT findings between Morgagni hernia and pleuropericardial fat. The aim of this study was to evaluate the useful CT findings for differentiating Morgagni hernia from pleuropericardial fat. Material and Method: We retrospectively analyzed CT scans of eight patients with Morgagni hernia and 20 patients with abundant pleuropericardial fat without peridiaphragmatic lesions. All CT scans were performed with coverage of the whole diaphragm in the inspiration state. We evaluated 1) the presence of the defect of the anterior diaphragm, 2) the interface between the lung and fat, 3) the angle between the chest wall and fat, 4) the continuity between the extrapleural fat and fat, 5) the presence of the vessels within fat, and 6) the presence of a thin line surrounding fat. Result: In all cases with Morgagni hernia, the defect of the anterior diaphragm was seen. The interface was well-defined, smooth, and convex to the lung. The angle with the chest wall was acute. The continuity with the extrapleural fat was not seen. In the cases with abundant pleuropericardial fat, the defect of the anterior diaphragm was seen in three (15%). The interface was usually irregular (n=10) and flat (n=17). The angle with the chest wall was variable. The continuity with the extrapleural fat, that was markedly increased in amount, was usually seen (n=16). The thin line surrounding fat was seen in four cases with Morgagni hernia, however, not seen in all cases with pleuropericardial fat. All of the above findings were statistically significant, however, vessels within fat was not significant to differentiate Morgagni hernia (n=8/8) from pleuropericardial fat (n=14/20). Conclusion: The useful CT findings of Morgagni hernia were fatty mass with sharp margin, convexity toward lung, acute angle with chest wall, and thin line surrounding hernia. Branching structure within fatty mass representing omental vessels that has been known as a characteristic finding of Morgagni hernia was not useful for differentiating Morgagni hernia from pleuropericardial fat.

Environmental characteristics on habitats of Viola diamantiaca Nakai and its RAPD analysis (금강제비꽃(Viola diamantiaca Nakai) 자생지의 환경특성과 RAPD 분석)

  • Seo, Won-Bok;Yoo, Ki-Oug
    • Korean Journal of Plant Taxonomy
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    • v.41 no.1
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    • pp.66-80
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    • 2011
  • This study investigated the environmental factors and conducted a RAPD analysis for a better understanding of the environmental characteristics and regional genetic variation in samples from 18 different areas of Viola diamantiaca. The habitats are mostly located on the slopes of mountains facing north at an altitude ranging from 614 m to 1,462 m above sea level with angles of inclination ranging from 3 degrees to 30 degrees. A total of 268 vascular plant taxa are identified in 35 quadrates of 18 habitats. The importance value of V. diamantiaca is 11.58%, and four highly ranked species, Sasa borealis (5.61%), Meehania urticifolia (5.21%), Ainsliaea acerifolia (3.62%), Pseudostellaria palibiniana (3.60%) are considered to have an affinity with V. diamantiaca in their habitats. The degree of their average species diversity is 1.36, while this metric for their evenness and dominance are 0.89 and 0.07, respectively. The average field capacity of the soil is 25.99%, with organic matter at 17.47%, and the pH is 5.19. The soil texture was confirmed as sandy loam of eleven and loam of seven. The result of the RAPD analysis, among 78 bands amplified with a primer, 64 (84.6%) showed polymorphism. Eighteen populations could be classified into five groups with similarity coefficient values ranging from 0.53 to 0.86. The Mt. Jiri population, which is geographically segregated, shows basal branching within the 18 populations. Five populations, including two in the southern district in Gangwon-do and three in Chungcheongbuk-do, form a distinct clade. Four populations in the central district of Gangwon-do and Mt. Bohyeon in the Gyeongsangbuk-do clade form a sister to the clade containing two populations in Gyeonggi-do and five populations of the northern district in Gangwon-do. The Mt. Gariwang population is placed between the southern district and the central district in the Gangwon-do clades.

Studies on the Phrenic Nerve Fibers in the dog II. On the Myelinated Fibers of the Intradiaphragmatic Branches (개의 횡격막신경섬유(橫隔膜神經纖維)에 관(關)한 연구(硏究) 제(第)II보(報) 횡격막신경(橫隔膜神經)의 횡격막내분기(橫隔膜內分技)의 유수섬유(有髓纖維)에 관(關)하여)

  • Ko, Kwang Doo;Yoon, Suk Bong;Lee, Joon Sup
    • Korean Journal of Veterinary Research
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    • v.9 no.1
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    • pp.1-9
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    • 1969
  • The experimental studies were performed to observe the characteristics of the myelinated nerve fibers of the tradiaphragmatic branches of phrenic nerves in dog In the work to be reported five mixed breed Korean dog were used, they were one year old and he body eights were about 12 Kgm. in average with healthy conditions. The specimens (nerve) were taken at the point of 1.5 cm posterior part from the left and right phrenic nerves entrance to the diaphragm, and the dorso-lateral and dorso-medial branches of phrenic nerves vere also, taken at the point of 0.5 cm distal part from their branching portion. The specimens were fixed for 24 hrs. in Flemming's solution, and embedded in paraffin. The paraffin section. were cut at 6 microns and stained with Walter's modification of Weigert-pal method for the myelinated fibers. Microphotographs were taken and enlarged into 750 times of the actual size, and the diameter of the photographic images of the myelinated fibers were measured by the scale on the transparent Percepex Plate. The following conclusions were made: 1. The percentage of distribution of the several intradiaphragmatic branches of phrenic nerves were as follows. Ventral branches were 34.97%, lateral branches were 37.90%, dorsal branches were 27.13%, and the dorsal branches were branched again into dorso-lateral branches(54.22%) and dorso-medial branches (45. 78%). 2. The mean value and S.D. of the total numbers of the myelinated nerve fibers at the left ventral branches were $490.80{\pm}12$, and at the right ventral branches were $486.6{\pm}13$. Total average cross sectional area on the left ventral branches were 38,000. $6{\pm}136{\mu}^2$, and the right ventral branches were $37,150.2{\pm}1.782{\mu}^2$. 3. The mean value and S.D. of the total numbers of the myelinated nerve fibers at the left lateral branches were $533.8{\pm}8$, and at the right lateral branches were $525.6{\pm}7$. Total average cross sectional area of the left lateral branches were $41,582{\pm}1,170{\mu}^2$, and the right lateral branches were $40,454.8{\pm}812{\mu}^2$. 4. The mean value and S.D. of the total numbers of the myelinated nerve fibers at the left dorsal branches were $378.2{\pm}14$, and at the right dorsal branches were $380.2{\pm}8$. Total average cross sectional area of the left dorsal branches were $27,771{\pm}1,256{\mu}^2$, and the right dorsal branches were $27,507.2{\pm}645{\mu}^2$. 5. The mean value and S.D. of the total numbers of the myelinated fibers at the left dorso-lateral branches were $205.4{\pm}15$, and at the right dorso-lateral branches were $210.6{\pm}17$. Total average cross sectional area of the left dorso lateral branches were $15,354. 8{\pm}1,519{\mu}^2$, and the right dorsal branches were $15,887{\pm}1,297{\mu}^2$. 6. The mean value and S.D. of the total numbers of the myelinated nerve fibers at the left dorso-medial branches were $175{\pm}14$, and at the right dorso-medial branches were $176.2{\pm}17$. Total average cross sectional area of the left dorso-medial branches were $13,037.4{\pm}944{\mu}^2$, and at the right dorso-medial branches were, $13,103{\pm}1,373{\mu}^2$. 7. The highest frequent distribution of the intradiaphragmatic branches of phrenic nerves were found in the $10-12{\mu}^2$ groups, and they were almost 30% of the total groups. 8. The largest fibers diameter were in the $14-16{\mu}^2$ groups, and these were shown the lowest frequent distribution. 9. The largest cross sectional area of the intradiaphragmatic branches of phrenic nerves were found in the $10-12{\mu}^2$ groups. 10. All of the intradiaphragmatic branches of phrenic nerves were unimodal which has a peak in the $10-12{\mu}^2$ groups.

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Planting Evaluations for the Landscaping Tree and Application Plan by Assessment Grade in the City Park - A Case Study of Haedoji Park, Songdo, Incheon Metropolitan City - (도시공원 조경수목 식재 평가 및 평가등급 적용 방안 - 인천광역시 송도 해돋이공원을 대상으로 -)

  • Han, Bong-Ho;Cho, Hun-Gum;Kwak, Jeong-In;Park, Seok-Cheol
    • Korean Journal of Environment and Ecology
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    • v.28 no.4
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    • pp.457-471
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    • 2014
  • This study aims to present an evaluation scheme to improve the problems in tree shapes and tree growth which were identified through shape and growth assessments of landscape trees currently planted in Haedoji Park, Songdo, Incheon Metropolitan City after plant structure status and propriety review for funtion of space and concept of planting. Suitability the planting concept was evaluated according to function of space. The result indicated that the shade planting areas accounted for 29.5% of the the shade spaces area. 58.7%, respectively planting areas of visual landscape. And 11.8%, respectively planting areas of buffer. Because the planting areas was lacked according to the park established spatial configuration of central facilities and the result of plant young trees. Plant structure status required consideration with plant structure, density, size, growth status for improve planting function. The tree assessment was performed on a total of 28 species and 600 trees of which 22 species and 209 trees were planted in the buffer zone, 8 species and 71 trees in the shade zone, 16 species and 266 trees in the visual landscape zone, and 4 species and 54 trees in the ecological landscape zone. The trees were divided into grades based on their assessment score and were statistically grouped by the functional zone in where they are planted and by tree species to verify their significance. The tree shape assessment was an average of 56.6 points and the tree growth assessment was an average of 76.0 points. Using the results of the tree assessments, the tree standards for each functional space were identified and a concept of optimum planting and cultivating was applied. When applying the shape assessment results by zone to the concept of planting, since trees for buffering require high functionality they received E's, the lowest grade; as trees for ecological landscaping require diverseness and naturalness, they received D's; since trees for shading require utilization, they received C's as trees with branching at the main stems were considered; and since trees for visual landscaping required aesthetical value, they received A's and B's. When applying the growth assessment results by zone to the concept of planting, based on planting foundations of favorable and poor, for buffering, visual landscaping, and ecological landscaping, trees from grades A to E could be planted, and for shading, trees from grades A to C could be planted. For a cultivation plan that could improve the growth of the trees, we proposed that the topography of the land be selected considering the tree's characteristics and that a method of pot seeding be used. Also, to improve the shape of the trees, we proposed that poles be used to improve the growth of vertically-straight stems, an appropriate planting density be applied for reasonable branch growth, manage tree shape to maintain good crowning, and better manage fertilization to maintain a reasonable crown density.

Taxonomic Characteristics of Korean-native Anacardiaceae (한국산(韓國産) 옻나무과(科)의 분류학적(分類學的) 연구(硏究))

  • Kim, Sam Sik;Chung, Jae Min
    • Journal of Korean Society of Forest Science
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    • v.84 no.2
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    • pp.151-165
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    • 1995
  • This study was conducted to establish a systematized taxonomic problems of through the leaf morphological characters and leaf venation patterns, and stomatal cell patterns and cell characteristics of abaxial and adaxial surface of the leaflets by SEM, of 6 native species in Korea and 2 foreign species of the Genus Rhus in the Family Anacardiaceae. The results obtained from this study are summarized as followings: 1. Morphological study measured 32 characters of leaves from herbarium specimen and field-collected samples for each species. The results of cluster analysis based on the Euclidean distance showed that the species could be classified into 3 groups: R. sylvestris. R. typhina, R. succedanea: R. trichocarpa. R. chinensis. R. verniciflua: and R. ambigua. R. radicans subsp. orientale, Analysis of principal components showed 5 groups: The major factors in the first principal component group was length of petiole of the terminal leaflets, that in the second group angle of left side in the terminal leaflet bash, that in the third group area ratio between first and terminal leaflets, that in the forth group angle ratio between right and left side in the terminal leaflet base, and that in the fifth group was angle of main and secondary vein at midrib of terminal leaflet. Cumulative contribution by the first, second and third principal component group was explained with 82.6%, a large percent of all information. 2. The leaf venation pattern investigated using soft X-ray photography revealed clado-and reticulo-camptodromous types according to branching angle of the secondary vein. And three groups by the developing degree of secondary vein were R. trichocarpa, R. ambigua. R. chinensis, R. typhina; R. radicans subsp. onentale, R. succedanea, R. verniciflua: and R. sylvestris. Classification key for the Rhus of Korean-native Anacardiaceae was made by the venation pattern and devevoping degree of the secondary vein. 3. The stomatal cell patterns were greatly classified into paracytic and anomocytic types, specific among species according to stomatal and subsidiary cell patterns, and various differences among the species was determined. Microstructure of the adaxial and abaxial surfaces could be divided into synclinal and anticlinal cell wall patterns, and were specific-species. Stomatal cells of R. chinensis were surrounded with characterized villus-like cells.

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Phylogenetic Analysis of Korean Native Aster Plants Based on Internal Transcribed Spacer (ITS) Sequences (ITS 염기서열을 이용한 한국산 참취속 식물의 유연관계분석)

  • Hong, Su-Young;Cho, Kwang-Soo;Yoo, Ki-Oug
    • Horticultural Science & Technology
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    • v.30 no.2
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    • pp.178-184
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    • 2012
  • This study was carried out to decide ITS (internal transcribed spacer) sequence of some Korean native $Aster$ species and to resolve their relationship among Korean native $Aster$, including $Kalimeris$, $Gymnaster$, $Heteropappus$ genus separated from $Aster$ in a previously study based on the pappus length. We registered 11 ITS sequences of $Aster$ species including $A.$ $glehni$ to GenBank and those sequences were used for the cluster analysis with $Kalimeris$ species. The size of ITS1 was varied from 248 to 256 bp, while ITS2 was varied from 220 to 222 bp. The G + C content of the ITS region ranged from 49.4 to 53.5%. Pairwise comparison results showed that the substitution rate of ITS1 and ITS2 region was 9% and 10%, respectively. $Kalimeris$ sensu strict substitution rate was lower than that of $Aster$ sensu strict species. The strict consensus parsimonious cluster analysis showed $A.$ $tripolium$ is the first branching from the clade and the next is $A.$ $scaber$. The $Kalimeris$ species except for the $A.$ $hispidus$ were grouped into the same clade with high bootstrap value (91%) within $Aster$. $Gymnaster$ and $Heteropappus$ that has been classified by morphological characters were also grouped into broad sense $Aster$ clade. These results implied these three genera could be merged together into $Aster$ based on the ITS sequences.

Assessment of Substantial Equivalence and Environmental Risk for Event Selection of Genetically Modified Chrysanthemum (국화의 GM 이벤트 선발을 위한 실질적 동등성 및 환경위해성 평가)

  • Lim, Jin Hee;Shim, Myung Syun;Oh, Kwan Hun;Seo, Ji Yeon;Lee, Su Young;Park, Sang Kun;Shin, Hak Ki;Cho, Myoung Rae
    • Horticultural Science & Technology
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    • v.32 no.4
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    • pp.517-524
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    • 2014
  • As an assessment of substantial equivalence and environmental risks of branchless chrysanthemum GM lines (LeLS69, LeLS76, LeLS80) developed in Korea, we investigated their degree of branchlessness and developmental characteristics including growth and flowering, as well as the stability of populations of insect pests and soil microbes. The GM lines were compared with a control variety, 'Jinba'. LeLS80 showed the highest level of branchlessness among the three GM lines, whereas abnormal axillary buds developed in lines LeLS76 and LeLS69. There were no significant differences between the growth characteristics of 'Jinba' and the GM lines. The branchlessness LeLS80 was increased during the summer season, suggesting that it is a useful germplasm for developing elite branchless cultivars. These GM lines showed no significant differences in the occurrence of five insect pests including aphids, mites, leaf miners, thrips, and whiteflies. Similarly, for soil microbes including bacteria and actinomycetes, there were no significant differences between 'Jinba' and the GM lines. However, there was a higher occurrence of fungi in the GM lines compared to 'Jinba'. Our findings suggest that 'Jinba' and the GM lines are substantially equivalent, although further monitoring studies are needed to confirm this.

Trace (Minor) Elemental and Isotopic Compositions of Calcitic Skeletons in the Eastern Coast and Cheju Island, Korea (한반도 동해안과 제주도 연안에서 발견되는 방해석질 각질의 미량원소, 부원소 및 탄소와 산소 동위원소 함량의 변화)

  • Ji, Ok-Mi;Woo, Kyung-Sik
    • The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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    • v.3 no.3
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    • pp.124-141
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    • 1998
  • The objectives of this study are to investigate the trace (minor) elemental and isotopic compositions of calcitic skeletons, such as barnacle, echinoid, branching and encrusting calcareous algae and oyster, from the eastern coast of Korean peninsula and Cheju Island, and to compare their variations with latitude, that is, the temperature of ambient seawater at which the skeletons grew. Articulated and encrusting red algae are composed of high-Mg calcite (7-21 mol% $MgCO_3$). Echinoids are also composed of high-Mg calcite (7-15 mol% $MgCO_3$). Whereas barnacles are composed of low-Mg calcite (1-5 mol% $MgCO_3$). The Mg compositions of articulated red algae, barnacle and oyster tend to increase with increasing water temperature, whereas those of encrusting red algae and echinoid do not show any trend. Sr compositions of articulated red algae, echinoid and barnacle tend to decrease with increasing water temperature, whereas those of encrusting red algae and oyster do not show any trend. Mn compositions of articulated red algae and encrusting red algae decrease with an increase of water temperature, whereas those of echinoid and barnacle do not show any trend. Fe compositions of articulated red algae tend to decrease with increasing water temperature, whereas those of encrusting red algae and oyster increase. Fe compositions of echinoid and barnacle do not show any trend with water temperature variations. Ba compositions of echinoid and low-magnesium calcitic skeletons do not show any trend with water temperature variations. Ba compositions of articulated red algae tend to decrease with increasing water temperature, whereas those of encrusting red algae increase. Cd and Pb compositions of all the skeletons tend to increase with increasing water temperature. Cu compositions of encrusting red algae increase with increasing water temperature, whereas articulated red algae, echinoid, barnacle and oyster do not show any trend. Zn compositions of high-magnesium calcitic skeletons and low-magnesium calcitic skeletons do not show any trend. Estimated water temperatures from oxygen isotopic data of all the skeletons except for the barnacle arc higher than the range of temperature of the shallow seawater of the East Sea and around Cheju Island. The oxygen isotopic compositions of oyster and echinoid are well clustered, and they do not show any trend with increasing water temperature. Therefore, this could reflect that the organisms have not secreted their shells in oxygen isotopic equilibrium with ambient seawater due to vital effect. Thus, the oxygen isotopic composition of barnacle can potentially be utilized for paleotemperature estimate whereas those of other organisms in this study may not be useful.

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