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http://dx.doi.org/10.7235/hort.2012.11139

Phylogenetic Analysis of Korean Native Aster Plants Based on Internal Transcribed Spacer (ITS) Sequences  

Hong, Su-Young (Highland Agriculture Research Center, National Institute of Crop Science, Rural Development Administration)
Cho, Kwang-Soo (Highland Agriculture Research Center, National Institute of Crop Science, Rural Development Administration)
Yoo, Ki-Oug (Department of Biological Sciences, Kangwon National University)
Publication Information
Horticultural Science & Technology / v.30, no.2, 2012 , pp. 178-184 More about this Journal
Abstract
This study was carried out to decide ITS (internal transcribed spacer) sequence of some Korean native $Aster$ species and to resolve their relationship among Korean native $Aster$, including $Kalimeris$, $Gymnaster$, $Heteropappus$ genus separated from $Aster$ in a previously study based on the pappus length. We registered 11 ITS sequences of $Aster$ species including $A.$ $glehni$ to GenBank and those sequences were used for the cluster analysis with $Kalimeris$ species. The size of ITS1 was varied from 248 to 256 bp, while ITS2 was varied from 220 to 222 bp. The G + C content of the ITS region ranged from 49.4 to 53.5%. Pairwise comparison results showed that the substitution rate of ITS1 and ITS2 region was 9% and 10%, respectively. $Kalimeris$ sensu strict substitution rate was lower than that of $Aster$ sensu strict species. The strict consensus parsimonious cluster analysis showed $A.$ $tripolium$ is the first branching from the clade and the next is $A.$ $scaber$. The $Kalimeris$ species except for the $A.$ $hispidus$ were grouped into the same clade with high bootstrap value (91%) within $Aster$. $Gymnaster$ and $Heteropappus$ that has been classified by morphological characters were also grouped into broad sense $Aster$ clade. These results implied these three genera could be merged together into $Aster$ based on the ITS sequences.
Keywords
cluster analysis; $Gymnaster$; $Heteropappus$; $Kalimeris$; taxonomy;
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