• The Korean Journal of Physiology
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• 제18권2호
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• pp.171-180
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• 1984

The death of cattle from acute bracken poisoning has been recognized for many years. Acute bracken poisoning is characterized by mucoidal nasal and anal hemorrhage, severe anorexia. pyrexia, gastric ulcer and myocardial damage. In 1958 Evans first suggested that clinical picture of bracken poisoning was very much similar to that of radiation injuries such as aplastic anemia, leucopenia, thrombocytopenia and increased capillary fragility. Bracken has been clearly demonstrated to contain a carcinogen as well as thiaminase. However, the nature of carcinogen in bracken has not definetely elucidated. Also it was warned by several workers that bracken could be a causative factor for stomach cancer in Korean and Japanese. It appears that little is known on the e(feet of bracken on the function of cardiovascular system. Therefore the present study was designed to explore effects of ethanol extract of raw and toiled bracken (RBEE:BBEE) on blood pressure in cats. Also studied was the mechanism underlying changed in blood pressure of cats by bracken. The result obtained were as follows; 1) Mean arterial blood pressure was invariably decreased following administration of either RBEE or BBEE. Tn general depressor responses to RBEE persisted longer than that to BBEE. Generally, depressor responses were proportional to debases of RBEE and BBEE administered. 2) After administration of 60 mg/kg RBEB and BBEE, blood Pressure decreased by $62.1{\pm}1.7mmHg$ and $68.0{\pm}3.0mmHg$, respectively. No change was observed between depressor responses to RBEE and that to BBEE. 3) Depressor responses to BBEE and RBEE were not affected by vagotomy, propranolol and regitine. 4) In atropinized animal depressor responses to BBEE and RBEE were reduced by 30-40% showing part of depressor response was resulted from cholinergic effect of bracken.

• 한국식생활문화학회지
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• 제6권1호
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• pp.43-54
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• 1991

우리나라 전통 음청류에 관한 역사적 배경을 고찰하고 8세기부터 1940년대까지의 기록으로부터 주류를 제외한 전통음료의 종류와 제조방법을 조사하였다. 옛 문헌에 기술된 음청류는 모두 70여가지로 집계되었으며 이들을 제조방법과 품질특성에 따라 순다류, 유사다류, 탕류, 장류, 숙수류, 미음류, 미식류, 식혜류, 수정과류, 화채류의 10가지로 분류하였다. 옛 문헌에서는 장(漿), 탕(湯), 청(淸), 다(茶)사이에 명확한 구분을 하고 있지 않았다. 밥을 유산균 발효시켜 물로 회석하여 만든 음료인 장(漿)은 신라, 고려시대에는 보편화된 음료로 보이나 오늘날에는 완전히 잊혀진 것으로 판단된다. 그 외의 음료는 18세기에 기술된 제조방법과 오늘의 제조방법 사이에 큰 차이를 보이지 않고 있다.

• 대한한방부인과학회지
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• 제20권1호
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• pp.50-60
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• 2007

Purpose : This investigation was undertaken to evaluate the anti-proliferation, in hexane, chloroform, ethyl acetate, butanol and water fraction from extract of Gyulyupsanbyonbang(GYSB) using MCF-7 human breast cancer cells. Methods : GYSB was added to distilled water(1500ml) and was boiled then filtered. The residue was suspended in distilled water and extracted with hexane, chloroform, ethyl acetate, butanol and water. MCF-7 cells were cultured in RPMI1640 complex badge, NIH3T3 was cultered in 37$^{\circ}$C, 5% moisture incubator of carbon dioxide with Dulbecco's Modified Eagle Medium(DMEM) supplemented with 10% fetal bovine serum and antibiotics. Cell cytotoxicity test about cancer cell was measured used MTT assay. Results: When it synthesizes a result, hexane and butanol fraction had shown anti-proliferation effect and safety together, and those anti-proliferation effect operating selectively appeared. Ethyl acetate fraction had anti-proliferation effect however, it was not selective. The Chloroform and water soluble fraction did not almost appear anti-proliferation effect. Conclusion : I can conclude that GYSB have anti-proliferation effect and safety together on MCF-7 cells. It suggest that GYSB may be useful for brest cancer patients.

• Journal of Microbiology and Biotechnology
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• 제24권4호
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• pp.522-533
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• 2014

Bionanotechnology has revolutionized nanomaterial synthesis by providing a green synthetic platform using biological systems. Among such biological systems, microalgae have tremendous potential to take up metal ions and produce nanoparticles by a detoxification process. The present study explores the intracellular and extracellular biogenic syntheses of silver nanoparticles (SNPs) using the unicellular green microalga Scenedesmus sp. Biosynthesized SNPs were characterized by AAS, UV-Vis spectroscopy, TEM, XRD, FTIR, DLS, and TGA studies and finally checked for antibacterial activity. Intracellular nanoparticle biosynthesis was initiated by a high rate of $Ag^+$ ion accumulation in the microalgal biomass and subsequent formation of spherical crystalline SNPs (average size, 15-20 nm) due to the biochemical reduction of $Ag^+$ ions. The synthesized nanoparticles were intracellular, as confirmed by the UV-Vis spectra of the outside medium. Furthermore, extracellular synthesis using boiled extract showed the formation of well scattered, highly stable, spherical SNPs with an average size of 5-10 nm. The size and morphology of the nanoparticles were confirmed by TEM. The crystalline nature of the SNPs was evident from the diffraction peaks of XRD and bright circular ring pattern of SAED. FTIR and UV-Vis spectra showed that biomolecules, proteins and peptides, are mainly responsible for the formation and stabilization of SNPs. Furthermore, the synthesized nanoparticles exhibited high antimicrobial activity against pathogenic gram-negative and gram-positive bacteria. Use of such a microalgal system provides a simple, cost-effective alternative template for the biosynthesis of nanomaterials in a large-scale system that could be of great use in biomedical applications.

• 한국식품과학회지
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• 제32권6호
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• pp.1306-1312
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• 2000

본 연구에서는 닭발 족편의 품질을 향상시키기 위해 닭살을 첨가한 족편에서 부재료의 최적 첨가 수준을 결정하였고, shelf-life를 측정하였으며, 마지막으로 원가 절감을 위해 닭살 대신 첨가할 수 있는 분말형 육수의 최적 수준을 결정하였다. 부재료 첨가에 따른 바람직한 정도에 대한 평가 결과, 전체적인 바람직한 정도, 닭고기 향미 및 양념의 바람직한 정도에 있어 후추는 일정수준의 첨가량까지는 값이 증가하다가 이후 감소함을 보였다. 이에 비해 생강은 뚜렷한 경향을 보이지 않았으나 후추와의 상호작용이 크게 나타났다. 반응표면분석을 통해 결정한 후추와 생강의 최적 첨가수준은 각각 0.33%와 0.1%이었다. 족편의 냉장 저장 중 관능적 특성 변화와 이화학적 특성 평가를 통해 냉장 족편의 shelf-life는 6일로 결정하였다. 또한 분말형 닭육수를 1.55%(w/w) 수준으로 첨가하는 경우 닭누린내나 탄내 같은 바람직하지 않은 성질은 적게 나타나면서, 익은 닭향미같은 바람직한 특성은 크게 증가시킬 수 있었다.

• 한국해양바이오학회지
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• 제14권2호
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• pp.61-68
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• 2022

This study aimed to investigate the hepatoprotective activities of the sea cucumber products, including extracts and hydrolysates, in vitro and in vivo. Dried sea cucumber, produced on the western coast of Korea, was boiled in water or 70% ethanol at 85℃ or 100℃ for 18 or 24 h, respectively, to extract bioactive compounds. The enzymatic hydrolysates were prepared by reacting the dried sea cucumber with pepsin or neutral protease (PNL) under optimal enzyme conditions. The anti-inflammatory effect of the samples was investigated using RAW 264.7 cells treated with lipopolysaccharide (LPS). The amount of nitric oxide (NO) was produced from the cells treated with LPS and each sample was compared. Therefore, the pepsin hydrolysate treatment decreased NO production compared to LPS sole treatment. Furthermore, the effects of the samples on cell injury in the hepatic cell line and bisphenolA-induced hepatic injury mouse model were investigated. The water extracts and the pepsin hydrolysates of sea cucumber significantly inhibited cell injury generated in the hepatocytes without cytotoxicity (p < 0.05), whereas the ethanol extracts were cytotoxic. However, these results indicate that the extracts and the enzymatic hydrolysates derived from sea cucumber can be used as beneficial materials for inhibiting liver damage.

• 현장농수산연구지
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• 제15권1호
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• pp.53-61
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• 2013

엿의 품질을 향상하기 위해 엿 제조시 기능성과 식이섬유함량이 풍부한 홍삼박의 첨가량별 엿의 제조방법 및 특성에 대한 연구를 실시한 결과 다음과 같다. 1. 증자쌀에 15% 엿기름 추출물을 증자쌀의 1.2배 첨가하여 70℃에서 12시간 당화시킨 후 농축하여 갱엿을 제조하고, 홍삼박을 수분함량 18% 정도로 건조하여 30mesh 이하로 파쇄한 홍삼박 분을 제조한다. 그리고 갱엿의 온도를 100℃ 정도로 유지하면서 홍삼박분을 넣어 잘 혼합하여 냉각시키면 홍삼박 엿을 얻을 수 있다. 2. 홍삼박 첨가량별 엿의 특성을 보면 홍삼박 첨가량이 증가 할수록 조직감의 Hardness와 Adhesiveness는 감소하였으며, 색차의 명도는 감소하였으나 적색도 및 황색도가 증가 하였다. 그리고 식미는 홍삼박 첨가로 무첨가 시 3.0 점의 "보통"이다 보다 향상되었는데 홍삼박 17.5% 첨가시 맛 4.2, 조직감 4.4 냄새 4.0으로 "좋다" 이상의 평가를 받았다.

• 한국수산과학회지
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• 제21권1호
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• pp.21-29
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• 1988

생합성조미료의 안전성문제 및 미각이 고급화, 다양화됨에 따라 천연소재를 원료로 하는 풍미계조미료의 수요가 늘고있다. 본연구는 천연분말수우프를 개발하기 위한 일련의 연구로서 분말가쓰오부시의 제조를 시도하였다. 즉 어체를 소형화함으로서 건조 시간을 단축시키는 한편 제품제조중 지질산화를 효율적으로 억제시키고 풍미면에서 재래식 가쓰오부시에 비해 손색이 없는 분말가쓰오부시를 제조하기 위한 가공조건을 구명하고, 아울러 제품제조중 정미 성분의 변화에 대하여 실험하였다. 동결저장 중인 가다랑어를 해동하여 머리, 내장을 제거한 후 필레로 만들어 1cm 두께로 절단하여 절단된 육을 가다랑어엑스분 중에서 20분간 자숙한 후 훈연실로 옮겨 $80^{\circ}C$에서 8시간 훈연 및 실온에서 15시간 엄증을 3차례 반복하였다. 이를 분쇄기로써 Somesh 크기로 분쇄하여 분말가쓰오부시제품을 제조하였다. 제품제조중 어체를 1cm 두께로 절단하여 가다랑어엑스분 중에서 자숙처리함으로서 제조중의 지질산화억제 및 제품의 풍미를 개선시킬 수 있었다. 분말가쓰오부시제품의 수분함량은 $11\~12\%$, 조지방함량은 $4.3\~4.8\%$이었다. 주요한 정미 성분으로는 건물량기준으로 IMP함량이 542.0mg/100g이었고, histidine(949.7mg/100g), anserine(441.4mg/100g), taurine(231.9mg/100g), carnosine (157.6 mg/100g), alanine(65.9mg/100g), lysine(50.2mg/100 g) 등의 유리아미노산함량이 2210.2mg/100g이었다. 불휘발성유기산중 lactic acid가 1087.2mg/100g으로 대부분을 차지하였고, 이외에 total creatinine이 592.1mg/100g, 미량의 betaine 및 TMAO로 이루어져 있었다. 무기질 중에서는 K, Mg, Na 및 Ca의 함량이 비교적 많았다. 제품제조중 유리아미노산과 유기산은 자숙중 상당량이 감소하나 훈건할 때 점차 증가하는 경향이었으며, 핵산관련물질 및 유기염기의 함량은 전공정을 통해 감소하였다. 본 분말가쓰오부시 제품은 재래식 가쓰오부시에 비해 제조공정 및 제조시간을 크게 단축시킬수 있었고 또한 풍미면에서도 손색이 없었다.

• 한국축산식품학회지
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• 제29권4호
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• pp.430-436
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• 2009

소나무껍질 추출물인 피타민은 다양한 생리활성을 발휘하는 항산화 및 항균제로서 알려져 있으며, 이러한 물질은 또한 인간의 삶에서 폭넓게 이용되었다. 브로일러 사료 내 피타민 프리믹스가 닭가슴살의 품질특성, TBARS 평가 및 관능평가에 미치는 영향을 조사하기 위해서 35일간의 실험을 수행하였다. 실험처리구 4개는 대조구, 항생제 0.1% 첨가구, 피타민 프리믹스 0.1%, 피타민 프리믹스 0.2% 첨가구로 구분하였다. 닭 가슴살의 단백질과 아미노산 함량은 각 처리구간 통계적인 유의차가 없었다. 피타민 프리믹스 첨가사료를 섭취한 닭고기의 pH는 대조구 및 항생제 첨가구 보다도 낮았으나, 닭 가슴살의 보수력은 피타민 프리믹스 첨가구가 대조구 및 항생제 첨가구에 비해서 유의적으로 높았다(p<0.05). 닭 껍질을 포함한 다리살의 TBARS 값은 저장일수가 증가함에 따라서 높아지는 경향이었으며, 피타민 프리믹스 첨가구가 대조구 및 항생제 첨가구에 비해서 유의적으로 낮았다(p<0.05). 항생제 첨가구를 제외한 삶은 닭고기의 관능평가 성적은 피타민 프리믹스 첨가구가 대조구에 비해서 유의적으로 높았다(p<0.05). 이 결과는 브로일러 사료 내 피타민 프리믹스가 닭고기의 품질, 맛, 향 그리고 저장성을 향상시킬 수 있음을 시사해준다.

• 대한화장품학회:학술대회논문집
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• 대한화장품학회 2003년도 IFSCC Conference Proceeding Book II
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• pp.26-31
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• 2003

Korean Ginseng is a medicinal herb which grows naturally in korea. an ancient country situated in north-eastern Asia. Its medical use was already well known to herb doctors in this region about five thousand years ago since the effectiveness of korean ginseng has been recognized through practical use for a long time. Korean Ginseng has always been regarded as a devine cure. The name "Ginseng" can be found in various medicinal books. many of which were written as early as B.C. 100. In the records of many chinese medical books. dating from the inception of publishing, it was noted that Korean Ginseng was of the highest level of quality. Korean Ginseng originally grew in the mountains of korea. However, this wild Korean Ginseng(js called SANSAM) could not meet the ever-increasing demands. and from the 16th century. it has been cultivated on farms for mass processing and supplying in korea(js called INSAM). It was already recognized in korea a long time ago(B.C. 57 - A.D. 668) that Korean Ginseng possessed the qualities of panacea, tonic and rejuvenator, and had other medicinal properties as well. The effectiveness of Korean Ginseng is widely recognized among south-eastern Asians as well as Chinese. As its effect has been proved scientifically. Korean Ginseng is now becoming the ginseng for all human beings in the world. Korean ginseng is differently called according to processing method. Dried thing is Insam(white ginseng), boiled or steamed is Hongsam(red ginseng). 장뇌삼(long headed ginseng) is artificially grown in the mountain no in field for a long time. So the body is thin and some long. but ingredients are concentrated. Korean wild ginseng(SANSAM) is rare in these days but we developed cosmetic ingredient. The scientific name of Korean Ginseng is Panax Ginseng. It has acknowledge as a natural mysterious cure among the notheastern peoples. because of its broad medicinal application. The origin of the word" Panax" derived from panacea. a Greek word meaning cure-all. According to the classification method of herb medicines in the Chinese medicinal book. "God-Farmer Materia Medica(A.D. 483-496) korean Ginseng was described as the superlative drug: panacea. tonic and rejuvenator. We studied skin immunological effect. collagen synthesis. cell growth and whitening effect of SANSAM extract. IN cosmetics.. SANSAM extract had skin fibroblast cell growth effect. recover damaged skin in the sun and protect fine wrinkle. Also. In hair product.. inhibits hairless, white hair.its hairless, white hair.