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http://dx.doi.org/10.15433/ksmb.2022.14.2.061

Extracts and Enzymatic Hydrolysates Derived from Sea Cucumber Stichopus japonicas Ameliorate Hepatic Injury in BisphenolA-treated Mice  

Sejeong, Kim (Geumsan Ginseng and Herb Development Agency)
Yun-Ho, Jo (Geumsan Ginseng and Herb Development Agency)
Bi-Oh, Park (Geumsan Ginseng and Herb Development Agency)
Dae-Seok, Yoo (Geumsan Ginseng and Herb Development Agency)
Doo-Ho, Kim (R&D Center, HuonsFoodience)
Min-Jung, Kim (R&D Center, HuonsFoodience)
Youn-Gil, Kwak (R&D Center, HuonsFoodience)
Jin-Seong, Kim (Geumsan Ginseng and Herb Development Agency)
Publication Information
Journal of Marine Bioscience and Biotechnology / v.14, no.2, 2022 , pp. 61-68 More about this Journal
Abstract
This study aimed to investigate the hepatoprotective activities of the sea cucumber products, including extracts and hydrolysates, in vitro and in vivo. Dried sea cucumber, produced on the western coast of Korea, was boiled in water or 70% ethanol at 85℃ or 100℃ for 18 or 24 h, respectively, to extract bioactive compounds. The enzymatic hydrolysates were prepared by reacting the dried sea cucumber with pepsin or neutral protease (PNL) under optimal enzyme conditions. The anti-inflammatory effect of the samples was investigated using RAW 264.7 cells treated with lipopolysaccharide (LPS). The amount of nitric oxide (NO) was produced from the cells treated with LPS and each sample was compared. Therefore, the pepsin hydrolysate treatment decreased NO production compared to LPS sole treatment. Furthermore, the effects of the samples on cell injury in the hepatic cell line and bisphenolA-induced hepatic injury mouse model were investigated. The water extracts and the pepsin hydrolysates of sea cucumber significantly inhibited cell injury generated in the hepatocytes without cytotoxicity (p < 0.05), whereas the ethanol extracts were cytotoxic. However, these results indicate that the extracts and the enzymatic hydrolysates derived from sea cucumber can be used as beneficial materials for inhibiting liver damage.
Keywords
Stichopus japonicas; Extraction; Enzymatic hydrolysis; Anti-inflammation; Liver damage;
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