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Influence of Grain Processing and Dietary Protein Degradability on Nitrogen Metabolism, Energy Balance and Methane Production in Young Calves

  • Pattanaik, A.K.;Sastry, V.R.B.;Katiyar, R.C.;Lal, Murari
    • Asian-Australasian Journal of Animal Sciences
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    • v.16 no.10
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    • pp.1443-1450
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    • 2003
  • Crossbred (Bos taurus${\times}$Bos indicus) calves were used from birth till 14 weeks of age to evaluate three sources of protein that differed in ruminal degradability viz. groundnut cake alone (HD) or in combination with cottonseed meal (MD) and meat and bone meal (LD), when fed along with two sources of non-structural carbohydrates viz. raw (R) and thermally processed (P) maize. Twenty four new born calves were arranged in six groups in a $3{\times}2$ factorial design and fed on whole milk up to 56 d of age. All the different calves received calf startes along with green oats (Avena sativa) from 14 d of age onwards free-choice. A metabolism trial of 6d starters duration, conducted after 90 d of experimental feeding, revealed greater (p<0.05) digestibility of DM, OM, total carbohydrates, NDF and ADF in calves fed on the P diets than on the R diets promoting greater (p<0.05) metabolizable energy intake. The digestibility of NDF was higher (p<0.01) on LD diets where as calves on MD diets exhibited significantly lower digestibility of ADF (p<0.01). The retention of nitrogen per unit metabolic body size was significantly (p<0.05) higher on the LD-P diet than on the diet HD-P which, in turn, was higher (p<0.05) than that of HD-R. Nitrogen retention as percentage of intake was significantly greater (p<0.05) on LD-P than on LD-R diets (52.2 vs. 36.4%). Also, P fed calves utilized nitrogen more efficiently than the R fed as shown by retention of significantly greater proportions of intake (47.4 vs. 40.9%) and absorbed (65.8 vs. 59.5%) nitrogen. Calorimetric evaluation of the diets through open-circuit respiration chamber revealed that the dietary treatments had no impact on methane production by calves. The intake of DE and ME was improved (p<0.01) because of maize processing resulting in greater (p<0.01) retention of energy. The protein degradability exerted no influence on the partitioning or retention of energy. A significant interaction between cereal and protein types was evident with respect to retention of both nitrogen (p<0.01) and energy (p<0.05). In conclusion, no discernible trend in the influence of cereal processing was apparent on the dietary protein degradability, but the positive effect of cereal processing on energy retention diminished with the increase in dietary undegradability.

Effect of Grape Pomace Powder, Mangosteen Peel Powder and Monensin on Nutrient Digestibility, Rumen Fermentation, Nitrogen Balance and Microbial Protein Synthesis in Dairy Steers

  • Foiklang, S.;Wanapat, M.;Norrapoke, T.
    • Asian-Australasian Journal of Animal Sciences
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    • v.29 no.10
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    • pp.1416-1423
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    • 2016
  • This study was designed to investigate the effect of grape pomace powder (GPP), mangosteen peel powder (MPP) and monensin on feed intake, nutrients digestibility, microorganisms, rumen fermentation characteristic, microbial protein synthesis and nitrogen balance in dairy steers. Four, rumen fistulated dairy steers with initial body weight (BW) of $220{\pm}15kg$ were randomly assigned according to a $4{\times}4$ Latin square design to receive four treatments. The treatments were as follows: T1 = control, T2 = supplementation with monensin at 33 mg/kg diet, T3 = supplementation with GPP at 2% of dry matter intake, and T4 = supplementation with MPP at 30 g/kg diet. The steers were offered the concentrate diet at 0.2% BW and 3% urea treated rice straw (UTRS) was fed ad libitum. It was found that GPP supplemented group had higher UTRS intake and nutrient digestibility in terms of neutral detergent fiber and acid detergent fiber than those in control group (p<0.05). Ammonia nitrogen ($NH_3-N$) and blood urea-nitrogen concentration were higher in monensin, GPP and MPP supplemented groups (p<0.05). Total volatile fatty acids and propionate in the GPP group were higher than those in the control group (p<0.05) while acetate concentration, and acetate to propionate ratio were decreased (p<0.01) when steers were supplemented with GPP, monensin, and MPP, respectively. Moreover, protozoal populations in GPP, MPP, and monensin supplementation were significantly lower than those in the control group (p<0.05), while cellulolytic bacterial population was significantly higher in the control group (p<0.05). Nitrogen retention, microbial crude protein and efficiency of microbial nitrogen synthesis were found significantly higher in steers that received GPP (p<0.05). Based on this study it could be concluded that the GPP has potential as an alternative feed supplement in concentrate diets which can result in improved rumen fermentation efficiency, digestibility and microbial protein synthesis in steers fed on treated rice straw.

Effect of water hardness on coffee composition and coffee preference by university students (물의 경도가 커피의 조성과 일반 대학생들의 커피 선호도에 미치는 영향)

  • Yoo, In-Kyung;Jang, Do-Hyeon;Kwon, Woo-Jin;Lee, Keon-Hee;Lim, Jinkyu
    • Korean Journal of Food Science and Technology
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    • v.52 no.5
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    • pp.435-440
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    • 2020
  • Water is a major ingredient and comprises over 98% content of coffee. In this study, coffees prepared with distilled water (L) and waters with medium (M) and high hardness (H) were investigated for organic acid, caffeine, and eugenol contents and analyzed by sensory evaluation. Organic acid content was the highest in H-coffee. The contents of chlorogenic acid and caffeine, the characteristic bitter taste molecules, were the highest in L-coffee. The level of eugenol, one of the representative aroma components, was the lowest in M-coffee but similar between L- and H-coffees. The sensory evaluation test, conducted on a university student group comprising 56 female and 47 male participants, showed that L-coffee was the most preferred (42% or higher), but there was no significant difference in bitterness, acidity, body, and balance. Results suggest that the preference index of coffee taste among university students may be different from that of coffee experts who prefer coffee extracted with water of certain hardness.

Effect of Dietary Brown Seaweed Levels on the Protein and Energy Metabolism in Broiler Chicks Activated Acute Phase Response (급성기 반응을 활성화한 육계 병아리에서 사료중 미역 제품 수준이 단백질과 에너지 대사에 미치는 영향)

  • Koh, T.S.;Im, J.T.;Park, I.K.;Lee, H.J.;Choi, D.Y.;Choi, C.J.;Lee, H.G.;Choi, Y.J.
    • Journal of Animal Science and Technology
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    • v.47 no.3
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    • pp.379-390
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    • 2005
  • Effects of dietary brown seaweed product levels on performance and metabolism of protein and energy were investigated in broiler chicks that were activated the acute phase response. One day old chicks were fed diets containing either 0.0(basal), 1.0, 2.0 or 4.0 % brown seaweed products for 3 weeks. The acute phase response was activated by injecting i.p. the Salmonella typhimurium lipopolysacharide(LPS) at $2^{nd}$ week of age. The acute phase response lowered nitrogen balance(NB)/ $kg^{0.75}$ (metabolic body size) and highered dietary ME values in birds fed diets containing brown seaweed product. Increase in dietary brown seaweed products levels lowered daily gain, and NB, uric acid nitrogen(UAN) excretion and ME utilization per $kg^{0.75}$ in chicks with the acute phase response. But the dietary brown seaweed product level did not affect the performance of 3 Week old broiler chicks that experienced the acute phase response. And the brown seaweed products 1.0 and 2.0 % diets lessened the feed intake reduction caused by the acute phase response in broiler chicks. The brown seaweed 2.0% diet increased NB / g diet or $kg^{0.75}$ and decreased the excretion of UAN/g diet or $kg^{0.75}$. This result indicated that the brown seaweed was able to interact with the acute phase response and increased protein retention via decreased breakdown of protein in birds fed brown seaweed 2.0% diet.

A Study on the Physiological Change of EEG by Olfactory Stimulation (후각자극에 의한 뇌파의 생리적 변화에 관한 연구)

  • 이동형;이재훈
    • Science of Emotion and Sensibility
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    • v.6 no.2
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    • pp.29-35
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    • 2003
  • In the oriental medicine, the illness of a human being is treated properly according to the strength and weakness of his/her physical constitution. In other words, the treatment principle of the oriental medicine is to balance the functions of the weak and strong inner parts of the physical constitution which can be classified into five characteristic elements: that is, the wood, fire, earth, mental, and water. An aroma therapy, one of traditional oriental medicine, uses pertinent perfumes to strengthen weak inner parts of the body, too. Although the remedy based on the five elements (in short, five element remedy) has revealed excellent clinical effects so far, it is still believed that the remedy is rather statistical and subjective than scientific. Thus, more systematic research needs to be done to prove it. In this paper, the correlation between perfume and physical constitutions has been formulated by grasping results of EEG through stimulation of lavender oil to both mental-type and fire-type persons. Also, the possibility of application for the five element remedy has been investigated. As a result, we found that the lavender oil helped to make strong gallbladder soften for fire-type individuals and to balance weak liver and strong gallbladder for mental-type persons. This agrees well with the results of the five element remedy practiced in the oriental medicine.

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A Study on the Operation of Export Credit Policy preparing for possible WTO ASCM Disputes (WTO 보조금 분쟁을 대비한 수출신용제도 운영방안에 관한 연구)

  • Oh, Won Suk;Kim, Pil Joon;Baek, Seung Taek
    • THE INTERNATIONAL COMMERCE & LAW REVIEW
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    • v.57
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    • pp.283-303
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    • 2013
  • When a trade conflict arises related to an officially supported export credit programme, The World Trade Organization(WTO), decides on whether the programme is a forbidden subsidy stipulated in the Agreement on Subsidies and Countervailing Measures(the ASCM Agreement). Korea was taken to the WTO panel two times for the export credit programme. One is the semiconductor case in 2002 and the other was the shipbuilding disputes in 2004. And, In 2012, the U.S. Commerce Department ruled K-SURE's export insurance for Korean refrigerator manufacturers as a forbidden subsidy even if the case was not taken to the WTO. This paper examines the significance of export credit programmes on the WTO ASCM Agreement and discusses how to operate these programmes so they would not infringe upon the Agreement by analyzing the actual cases of WTO subsidy conflicts that involved Korean enterprises in relation to export credit programmes for the purpose of determining the related issues and impacts. From this research the results were as follows: First, on whether export credit is a prohibited subsidy, the deciding factor was whether a benefit has been conferred to the beneficiary. On the presence of a benefit, the WTO panel used market benchmarks as the main criteria. Thus, official export credit agencies(ECAs) should be careful not to provide export credit support which had been granted to the beneficiary at better than market terms. Second, in the case of export credit, the special status of ECA as a public body receiving government support itself does not constitute a subsidy. However, caution must be taken not to provide export credit that may lead to WTO ASCM subsidy conflicts involving a certain exporter or industry by setting up clear and valid regulations and fair work processes in the operation of export credit programmes. Third, item (j) of Annex I cannot be interpreted reversely as this item is for interpreting the presence of a prohibited subsidy, not the presence of a benefit. Thus, an export credit program that confers a financial contribution, a benefit and specificity, could qualify as a prohibited subsidy. Fourth, ECAs not only have to maintain long-term account balance but also introduce additional measures to meet this long-term balance such as a clear and systematic premium system. Finally, export credit programmes that are not defined in item (j) of Annex I of the ASCM Agreement would not deemed as an prohibited export subsidy as long as the continued support of the programmes are not being forced.

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Effects of Supplementing with Single or Multiple Trace Minerals on Growth Performance, Fecal Mineral Excretion and Nutrient Utilization in Pullets from 1 to 18 Weeks of Age

  • Zhong, L.L.;Yao, J.H.;Cheng, N.;Sun, Y.J.;Liu, Y.R.;Wang, Y.J.;Sun, X.Q.;Xi, H.B.
    • Asian-Australasian Journal of Animal Sciences
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    • v.20 no.6
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    • pp.976-982
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    • 2007
  • This study investigated whether supplemental Cu, Fe, Zn, and Mn are needed in a practical diet for pullets. Four hundred and twenty females of an egg-laying strain (1-d-old, Lohmann Brown Layer) were randomly distributed into 4 groups, consisting of 7 replicates of 15 birds each. During the 18-week experimental period, chicks were given three basal diets in sequence, each with single or multiple Mn, Zn and Cu supplementation to improve the mineral balance gradually. In the Control, no Mn, Zn, and Cu were added; in the single Mn supplemented group (sMn) Mn was added to 120, 60, and 60 mg/kg for 1-6, 7-12, and 13-18 weeks of age, respectively; in the multiple Mn and Zn supplemented group (mMnZn), Mn was added to 180, 90, and 90 mg/kg and Zn was added to 120, 105, and 105 mg/kg for 1-6, 7-12, and 13-18 weeks of age, respectively; in the multiple Mn, Zn, Cu supplemented group (mMnZnCu), Mn, Zn, and Cu were added to the same multiple of basal Fe concentration relative to NRC (1994) recommendations. Energy and protein metabolizability were determined by subtracting energy/protein intake by energy/protein excretion (from both feces and urine) and dividing by energy/protein intake. There were no statistically significant differences between groups in terms of feed intake, final body weight or tibia length throughout the experiment. Optimal growth performance was observed in the Control, while adding trace minerals to basal diets tended to result in decreased productive performance. Protein metabolizability was increased by mMnZn and mMnZnCu treatments, but energy metabolizability was not affected. Concentrations of Mn, Zn, Cu in excreta varied greatly related to dietary content, and the retentions of Cu, Fe, Zn and Mn were all increased due to the improvement of mineral balance. Based on these results, it is suggested that the concentrations of Cu, Fe, Zn and Mn in typical basal diets used in this study were adequate for normal growth for pullets from 1 to 18 weeks of age.

Assessment of Isokinetic Muscle Function, Balance and Flexibility in Elite High School Kayakers (고등학교 엘리트 카약 선수들의 등속성 근기능·균형성·유연성 측정 및 평가)

  • Shin, Kwang-Taek;Sohn, Jee-Hoon
    • Journal of Digital Convergence
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    • v.16 no.6
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    • pp.407-416
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    • 2018
  • The purpose of this study is to evaluate elite high school kayaker's iso-kinetic muscle function and provide physical training advices. Biodex system IV, Biodex stabilizer system, Inbody 3.0 and CNP-5403 are used to investigate 11 kayaker's muscle function(peak torque, average power and work fatigue), dynamic balance, body compositions, and flexibility. S4, S5, S8, S10, S11 are needed to train shoulder muscle strength and power. S5, S6, S10, S11 are needed to strengthen shoulder muscle endurance. S5, S7, S8, S10, S11 are needed to train knee muscle strength and power. S1, S4, S5, S10 are needed to strengthen knee muscle endurance. S3, S10, S11 are needed to train wrist muscle endurance. Based on these results we should establish an muscle function enhancing program with periodic inspection for elite kayakers. And standardized training program should be sublated.

Monensin Enriched Urea Molasses Mineral Block on Feed Intake, Nutrient Digestibility and Blood Glucose in Cattle Fed on Wheat Straw Based Diet

  • De, Debasis;Singh, G.P.
    • Asian-Australasian Journal of Animal Sciences
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    • v.15 no.11
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    • pp.1579-1584
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    • 2002
  • Twelve adult male crossbred (Sahiwal${\times}$Holstein Friesian) cattle were distributed into four groups of three each on body weight basis. Animals were given wheat straw as a basal diet. The animals of group I and II were supplemented with concentrate mixture and animals of group III and IV were supplemented with cold processed urea molasses mineral block (UMMB). Thirty mg monensin/day/animal was supplemented to the animals of group II and 35 ppm monensin were incorporated in the UMMB supplemented to the animals of group IV. Vit.A and D mixture was given to all the animals once a week. Dry matter (DM) intake (kg/d) through wheat straw was 19.0 percent higher in the UMMB (without monensin) supplemented group (group III) than those of the concentrate mixture (without monensin) supplemented group i.e. group I. Total DM intake (kg/d) was lower in the monensin supplemented groups than those of non-supplemented groups though differences were not statistically significant. Digestible dry matter, organic matter (OM), crude protein (CP) and total digestible nutrients (TDN) intake were similar in all the groups. Average block consumption for 45 d period in the group III (0.95 kg/d) and group IV (0.84 kg/d) did not differ significantly. DM digestibility (%) was significantly (p<0.01) higher in the group II (58.9) as compared to the group I (52.7) and group III (54.0) but similar to the group IV (57.2). OM digestibility was also significantly (p<0.05) higher in the group II (63.2) as compared to that of the group I (54.9) but similar to the group III (57.8) and IV (59.2). Ether extract (EE) digestibility was significantly (p<0.01) higher in the group I (76.9) and II (80.3) as compared to the group III (59.87) and IV (55.77). Nitrogen free extract (NFE) digestibility was significantly (p<0.05) higher in the group II (62.38) as compared to that of the other groups. Crude protein (CP) and crude fibre (CF) digestibilities were not affected either due to UMMB or monensin. Nitrogen balance did also not differ significantly among the groups. However, Ca and P balance (g/d) in the group III (3.1, 1.3) and IV (3.0, 1.4) were significantly (p<0.01) higher than those of the group I (0.6, 0.2) and II (0.4, 0.3). Blood glucose (mg/100ml) was significantly (p<0.01) higher in the group II (65.2) and IV (65.2) as compared to the group I (55.2) and group III (53.9). Plasma urea-N level (mg/100 ml) in the group III (19.0) and IV (17.8) were significantly (p<0.01) higher than that of the group I (10.5) and II (12.3). So, monensin supplementation either with cold process UMMB or concentrate mixture did not show any additional effect on feed intake and digestibility but increases blood glucose level in adult cattle.

The Optimum Methionine to Methionine Plus Cystine Ratio for Growing Pigs Determined Using Plasma Urea Nitrogen and Nitrogen Balance

  • Qiao, Shiyan;Piao, Xiangshu;Feng, Zhanyu;Ding, Yuhua;Yue, Longyao;Thacker, P.A.
    • Asian-Australasian Journal of Animal Sciences
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    • v.21 no.3
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    • pp.434-442
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    • 2008
  • The objective of this study was to determine the optimum ratio of methionine to methionine plus cystine for growing pigs. A nitrogen balance trial was conducted using a total of 21 barrows (Large WhiteLandrace) over two replicates. The initial body weight was $20.36{\pm}1.22kg$ (mean${\pm}$SD) in the first replicate and $23.54{\pm}1.02kg$ (mean${\pm}$SD) in the second. For each replicate, the 21 pigs were randomly assigned to one of seven dietary treatments with three observations per treatment. The diets included a methionine and cystine-deficient basal diet with all other essential nutrients meeting nutrient requirements and six diets formulated with graded levels of DL-methionine (0.00, 0.03, 0.06, 0.10, 0.13, 0.16%) and $L-Cystine{\cdot}HCl{\cdot}H_2O$ (0.19, 0.15, 0.11, 0.07, 0.04, 0.00%). This resulted in ratios of methionine to methionine plus cystine of 41.3, 29.6, 35.3, 41.2, 46.0, 51.6 and 57.5%. Each experimental period lasted 12 days consisting of a seven-day adaptation period followed by a five-day total collection of urine and feces. During the collection period, pigs were fed 900 g/day for the first replicate and 1,200 g/day for the second replicate. The feed was provided in three equal portions at 0800, 1500, and 2200 h daily. Pigs had ad libitum access to water after feeding. There was a linear (p<0.01) and quadratic (p<0.01) effect on daily gain and feed conversion as the ratio of methionine to methionine plus cystine increased. Pigs receiving the diets providing a methionine to methionine plus cystine ratio of 51.6% had the best daily gain and feed conversion. Plasma urea nitrogen was also lowest for this treatment. Nitrogen retention increased (p<0.01) as the relative proportion of methionine increased up to 51.6% and then a downward trend occurred at 57.5%. The quadratic regression model, as well as one- and two- slope regression line models, were used to determine the optimum ratio of methionine to methionine plus cystine. Eliminating the 35.3% methionine to methionine plus cystine treatment resulted in $R^2$ values in excess of 0.92. The optimal ratio of methionine to methionine plus cystine was estimated to be 54.15% for nitrogen retention and 56.72% for plasma urea nitrogen.