• 한국해양학회지:바다
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• 제14권4호
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• pp.229-239
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• 2009

2007년과 2008년 Cochlodinium 적조발생규모에 영향을 미친 주요원인을 파악하기 위하여 적조발생기간 중 해양학적, 기상학적 특징 등을 비교분석하였다. 2007년도 남해연안과 동해연안에서 115억원의 수산피해를 일으킨 대규모 적조발생원인은 적조발생기간 중 집중강우, 냉수대발생으로 인한 연안용승, 수온성층 약화로 인한 연안역의 풍부한 영양염과 낮은 포식 동물플랑크톤 때문으로 해석되었다. 한편, 2008년도 남해안 일부 연안에서 수산피해없이 발생한 소규모 적조원인은 적조발생기간 중 지속된 가뭄과 빈영양 외양수가 연안역에 강하게 영향을 미침으로써 연안역의 낮은 영양염과 예년보다 월등히 높은 밀도의 포식 동물플랑크톤 영향 때문으로 풀이되었다. 따라서, 영양염 농도, 외양수 영향, 포식 동물플랑크톤은 2007년과 2008년 적조발생 규모에 많은 영향을 미친 것으로 추정되었다.

• 한국환경과학회:학술대회논문집
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• 한국환경과학회 2016년도 정기학술대회 발표논문집
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• pp.183-183
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• 2016

• 한국어업기술학회:학술대회논문집
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• 한국어업기술학회 2000년도 추계수산관련학회 공동학술대회발표요지집
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• pp.143-144
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• 2000

자연황토를 해수와 혼합 분쇄, 적조발생 해역에 정확히 살포하여 보다 경제적으로 황토를 살포하는 황토살포기 및 황토가 pH값의 변화에 따라 활성도가 달라지는 연구결과에 착안하여 해수를 전기분해하여 생성되는 전해수(산성수 및 알칼리수)에 황토를 혼합하여 황토를 활성화시켜 적조구제효율을 높이는 방법에 대하여 연구를 실시, 보다 효율적이며 친환경적인 적조구제 기술을 개발하였다. (중략)

• Ecology and Resilient Infrastructure
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• 제6권1호
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• pp.77-77
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• 2019

• Journal of Microbiology and Biotechnology
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• 제18권10호
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• pp.1621-1629
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• 2008

Harmful algal blooms (HABs), commonly called red tides, are caused by some toxic phytoplanktons, and have made massive economic losses as well as marine environmental disturbances. As an effective and environment-friendly strategy to control HAB outbreaks, biological methods using marine bacteria capable of killing the harmful algae or algicidal extracellular compounds from them have been given attention. A new member of the $\gamma$-Proteobacteria, Hahella chejuensis KCTC 2396, was originally isolated from the Korean seashore for its ability to secrete industrially useful polysaccharides, and was characterized to produce a red pigment. This pigment later was identified as an alkaloid compound, prodigiosin. During the past several decades, prodigiosin has been extensively studied for its medical potential as immunosuppressants and antitumor agents, owing to its antibiotic and cytotoxic activities. The lytic activity of this marvelous molecule against Cochlodinium polykrikoides cells at very low concentrations ($\sim$l ppb) was serendipitously detected, making H. chejuensis a strong candidate among the biological agents for HAB control. This review provides a brief overview of algicidal marine bacteria and their products, and describes in detail the algicidal characteristics, biosynthetic process, and genetic regulation of prodigiosin as a model among the compounds active against red-tide organisms from the biochemical and genetic viewpoints.

• 환경생물
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• 제27권3호
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• pp.244-251
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• 2009

두 가지 다른 기질의 담체를 이용하여 살조세균 Pseudomonas fluorescens HYK0210-SK09의 포집율과 microcosm에서 Stephanodiscus hantzschii의 살조능을 파악하였다. Active carbon polyvinyl alcohol (ACPA)담체가 cellulose sponge (CS)담체보다 SK09를 높게 포집하였다. 이를 이용한 microcosm에서 ACPA담체가 CS담체보다 S. hantzschii의 살조능이 높았으며 지속적이었다. 특히 ACPA담체에서는 낮은 전기전도도를 나타내어 S. hantzs-chii 분해에 따른 용출된 이온들을 흡수하고 있음을 판단할 수 있었다. 따라서 ACPA담체를 이용한 유해조류 제어는 지속적 제어와 함께 생태계의 교란을 최소화할 것으로 판단된다.

• 한국환경과학회지
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• 제19권12호
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• pp.1385-1395
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• 2010

Harmful dinoflagellate Cochlodinium polykrikoides blooms have been frequently occurred in coastal areas of the East Sea since 1995. We compared the oceanic conditions in years 1995, 2001 and 2003 when the C. polykrikoides bloom was strong, and in years 1998 and 2004 when the C. polykrikoides bloom was not appeared. We studied temporal and spatial variation of upwelling and geostrophic currents on the western channel of Korean Strait, an entrance of the East Sea. The period and occurrence area of C. polykrikoides bloom was depended on variation of upwelling in summer. In the distributions of geostrophic current, southward current was dominant near the coast in August, 1998 and 2000. Whereas northward current was dominant near and off the coast in August, 1995 and 2003 which the C. polykrikoides bloom was strong. When compared dominant phytoplankton of the coastal areas in each year, Kuroshio indicator species Proboscia alata and Chaetoceros affine were dominant, respectively, in 2001 and 2003 at every stations. However, the dominant species was variable at each coastal area in 1998 and 2000. In 2003, the abundance of Sagitta elegans which is known as the cold water indicator was low, but the abundance of S. enflata, warm water indicator, was very high in Gangneung compared to Sokcho. It seemed that the distribution of S. elegans is restricted by strong warm water current. In conclusion, it was estimated that the distribution of C. polykrikoides bloom in the coastal area of the East Sea was closely related with the strength of East Korea Warm Current and upwelling.

• 한국환경과학회지
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• 제17권4호
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• pp.439-449
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• 2008

Algicidal bacterium was isolated from sea water during the declining period of Cochlodinium polykrikoides blooms and this bacterium had a significant algicidal activity against C. polykrikoides. In this study, algicidal bacterium was identified on the basis of biochemical and chemotaxonomic characteristics, and analysis of 16S rDNA sequences. The algicidal bacterium showed 98.6% homology with Micrococcus luteus ATCC $381^T$. Therefore, this bacterium was designated Micrococcus luteus SY-13. The optimal culture conditions of the algicidal bacterium was $25^{\circ}C$, initial pH 8.0, and 3.0% NaCl concentration. M. luteus SY-13 is assumed to produce secondary metabolites which have algicidal activity. When 10% culture filtrate of this strain was applied to C. polykrikoides ($1.0\;{\times}\;10^4\;cells/ml$) cultures, over 98% of C, polykrikoides cells were destroyed within 6 hours. The culture filtrate of M. luteus SY-13 exhibited similar algicidal activity after heat-treatment at $121^{\circ}C$ for 15 min. While algicidal activity remained in filtrates with pH adjusted to 8.0, loss of algicidal activity occurred when the pHs of filtrates were adjusted to over 9.0 or heat-treated at $121{\times}180^{\circ}C$ for 1 hour. M. luteus SY-13 showed significant algicidal activities against C. polykrikoides (98.9%) and a wide algicidal range against various harmful algal bloom (HAB) species. However, there was no algicidal effect on diatom and marine livefood organisms except Isocrysis galbana. These results suggest that M. luteus SY-13 could be a candidate for use in the control of HABs.

• ALGAE
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• 제20권2호
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• pp.113-120
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• 2005

The freshwater blooms mainly blue-green algal blooms occur frequently in the lower Naktong River in summer, which provoke many socio-economical problems; therefore, the early detection of bloom events are demanding through the quantitative and qualitative analyses of blue green algal species. The in vivo fluorescence properties of cultured strains of Microcystis aeruginosa, M. viridis, M. wesenbergii, M. ichthyoblabe, Anabaena cylindrica, A. flos-aquae, and Synedra sp. were investigated. Wild phytoplankton communities of the lower Naktong River were also monitored at four stations in terms of their standing stocks, biomass and fluorescence properties compared with its absorption spectram. The 77K fluorescence emission spectra of each cultured strains normalized at 620 nm was very specific and enabled to detect of blue green algal biomass qualitatively and quantitatively. The relative chlorophyll a concentration determined by chlorophyll fluorescence analysis method showed significant relationship with chlorophyll a concentration determined by solvent extraction method ($R^2$ = 0.906), and the blue-green algal cell number determined by microscopic observation ($R^2$ = 0.588), which gives insight into applications to early detection of blue green algal bloom.

• 한국어병학회지
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• 제16권3호
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• pp.193-201
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• 2003

Mastoparan B (MPB)는 벌독으로부터 정제된 양친매성 $\alpha$-helical 구조를 취하면서 14개의 아미노산 잔기로 구성된 염기성 항균성 펩타이드로서 여러가지 생물막과 작용한다. 본 연구에서는 우리 나라 연안의 적조 (HABs, harmful algal blooms)를 일으키는 4종의 적조생물 (Alexandrium tamarense, Chattonella marina, Cochlodinium polykrikoides 및 Gymnodinium catenatum)에 대한 MPB의 살조효과를 조사하였다. MPB의 4종의 적조생물에 대한 살조효과는 31.3 $\mu{g}$/mL부터 500 $\mu{g}$/mL에서 세포의 lysis 또는 ecdysis와 같은 형태로 현미경으로 관찰할 수 있었다. 또한 MPB는 C. marina 및 C. polykrikorides에 대해서 A. tamarense와 G. catenatum보다 더욱 강한 살조효과를 나타내었다. 이러한 MPB의 HABs에 대한 살조효과연구는 새로운 살조물질을 개발하기 위한 자료가 될 것으로 생각된다.