Journal of Environmental Science International (한국환경과학회지)

The Korean Environmental Sciences Society (한국환경과학회)
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Characteristics of Algicide Produced by Micrococcus luteus SY-13 Inhibiting Cochlodinium polykrikoides and the Effects on Marine Organisms

적조생물 Cochlodinium polykrikoides를 저해하는 Micrococcus luteus SY-13이 생산하는 살조물질의 특성과 해양생물에 미치는 영향

  • Kim, Min-Ju (Department of Microbiology, Pusan National University) ;
  • Jeong, Seong-Yun (Korea Bio-IT Foundry Center, Pusan National University) ;
  • Cha, Mi-Sun (School of Mechanical and Aerospace Engineering, Seoul National University) ;
  • Lee, Sang-Joon (Department of Microbiology, Pusan National University)
  • 김민주 (부산대학교 미생물학과) ;
  • 정성윤 (부산대학교 한국 Bio-IT 파운드리 센터) ;
  • 차미선 (서울대학교 기계항공공학부) ;
  • 이상준 (부산대학교 미생물학과)
  • Published : 2008.04.30
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Abstract

Algicidal bacterium was isolated from sea water during the declining period of Cochlodinium polykrikoides blooms and this bacterium had a significant algicidal activity against C. polykrikoides. In this study, algicidal bacterium was identified on the basis of biochemical and chemotaxonomic characteristics, and analysis of 16S rDNA sequences. The algicidal bacterium showed 98.6% homology with Micrococcus luteus ATCC $381^T$. Therefore, this bacterium was designated Micrococcus luteus SY-13. The optimal culture conditions of the algicidal bacterium was $25^{\circ}C$, initial pH 8.0, and 3.0% NaCl concentration. M. luteus SY-13 is assumed to produce secondary metabolites which have algicidal activity. When 10% culture filtrate of this strain was applied to C. polykrikoides ($1.0\;{\times}\;10^4\;cells/ml$) cultures, over 98% of C, polykrikoides cells were destroyed within 6 hours. The culture filtrate of M. luteus SY-13 exhibited similar algicidal activity after heat-treatment at $121^{\circ}C$ for 15 min. While algicidal activity remained in filtrates with pH adjusted to 8.0, loss of algicidal activity occurred when the pHs of filtrates were adjusted to over 9.0 or heat-treated at $121{\times}180^{\circ}C$ for 1 hour. M. luteus SY-13 showed significant algicidal activities against C. polykrikoides (98.9%) and a wide algicidal range against various harmful algal bloom (HAB) species. However, there was no algicidal effect on diatom and marine livefood organisms except Isocrysis galbana. These results suggest that M. luteus SY-13 could be a candidate for use in the control of HABs.

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References

  1. Kim H. G., 1997, Recent harmful algal blooms and mitigation strategies in Korea, Ocean Res., 19,185-192
  2. Lee Y. S., 2006, Factors affecting outbreaks of high-density Cochlodinium polykrikoides red tides in the coastal seawaters around Yeosu and Tongyeong, Korea, Mar. Pollut. Bull., 52, 1249-1259 https://doi.org/10.1016/j.marpolbul.2006.02.024
  3. Lee Y. S., Lee S. Y., 2006, Factors affecting outbreaks of Cochlodinium polykrikoides blooms in the coastal areas of Korea, Mar. Pollut. Bull., 52, 626-634 https://doi.org/10.1016/j.marpolbul.2005.10.015
  4. 박치현, 이병호, 2006, 적조방제용 황토살포가 연안 저서생태계에 미치는 영향, 한국환경과학회지, 11, 1035-1043 https://doi.org/10.5322/JES.2006.15.11.1035
  5. 최희구, 김평중, 이원찬, 윤성종, 김학균, 이흥재, 1998, 황토의 유해성 적조생물 Cochlodinium 종의 제거 효과, 한국수산학회지, 31, 109-113
  6. 윤종섭, 김승현, 윤조희, 2003, 황토살포에 의한 적조생물의 제거, 대한환경공학회지, 25, 358-363
  7. 김성재, 2000, 적조생물의 구제-2.황토에 의한 적조생물의 응집제거, 한국수산학회지, 33, 455-462
  8. 박치현, 이병호, 2007, 적조방제용 황토살포의 양을 줄일 수 있는 첨가제에 대한 연구, 한국환경과학회지, 16, 745-750 https://doi.org/10.5322/JES.2007.16.6.745
  9. Yoshinaga I., Kawai T., Takeuchi T., Ishida Y., 1995, Distribution and Fluctuation of bacteria inhibiting the growth of a marine red tide plankton Gymnodinium mikimotoi in Tanabe bay, Fish. Sci., 61, 780-786 https://doi.org/10.2331/suisan.61.780
  10. Fukami K. T., Nishijima T., Hata Y., 1997, Stimulative and inhibitory effects of bacteria on the growth of microalgae, Hydrobiologia, 385, 185-191
  11. Imai I., Ishida Y., Sakaguchi K., Hata Y., 1995, Algicidal marine bacteria isolated from northern Hiroshima Bay, Japan. Fish. Sci., 61, 628-636 https://doi.org/10.2331/fishsci.61.628
  12. Fukami K., Sakaguchi K., Kanou M., Nishijima T., 1996, Effect of bacterial assemblages on the succession of blooming phytoplankton from Skeletonema costatum to Heterosigma akashiwo. In Harmful and toxic algal blooms ed. Yasumoto, T., Oshima, T., FuKuyo, Y., pp. 335-338, Paris: Intergovernmental Oceanographic commission of UNESCO
  13. Kim M. C., Yoshinaga I., Imai I., Nagasaki K., Itakura S., Ishida Y., 1998, A close relationship between algicidal bacteria and termination of Heterosigma akashiwo (Raphidophyceae) bloom in Hiroshima Bay, Japan., Mar. Ecol. Prog. Ser., 170, 25-32 https://doi.org/10.3354/meps170025
  14. Uchida T., Toda S., Matsuyama Y., Yamaguchi M., Kotani Y., Honjo T., 1999, Interactions between the red tide dinoflagellates Heterocapsa circularisquama and Gymnodinium mikimotoi in laboratory culture, J. Exp. Mar. Bio. and Ecol., 241, 285-299 https://doi.org/10.1016/S0022-0981(99)00088-X
  15. Kim D., Oda T., Muramatsu T., Kim D., Matsuyama Y., Honjo T., 2002, Possible factors responsible for the toxicity of Cochlodinium polykrikoides, a red tide phytoplankton, Comp. Biochem. Physiol. C-Toxicol. Pharmacol., 132, 415-423 https://doi.org/10.1016/S1532-0456(02)00093-5
  16. Jeong S. Y., Ishida K., Ito Y., Okada S., Murakami M., 2003, Bacillamide, a novel algicide from the marine bacterium, Bacillus sp. SY-1, against the harmful dinoflagellate, Cochlodinium polykrikoides, Tetrahedron Lett., 44, 8005-8007 https://doi.org/10.1016/j.tetlet.2003.08.115
  17. Ahn C. Y., Joung S. H., Jeon J. W., Kim H. S., Yoon B. D., Oh H. M., 2003, Selective control of cyanobacteria by surfactin-containing culture broth of Bacillus subtilis C1, Biotechnol. Lett., 25, 1137-1142 https://doi.org/10.1023/A:1024508927361
  18. Mu R. M., Fan Z. Q., Pei H. Y., Yuan X. L., Liu S. X., Wang X. R., 2007, Isolation and algae-lysing characteristics of the algicidal bacterium B5, Journal of Environmental Sciences, 19, 1336-1340. https://doi.org/10.1016/S1001-0742(07)60218-6
  19. Doucette G. J., McGovern E. R, Babinchak J. A., 1999, Algicidal bacteria active against Gymnodinium breve (Dinophyceae). I. Bacterial isolation and characterization of killing activity, J. Phycol., 35, 1447-1454 https://doi.org/10.1046/j.1529-8817.1999.3561447.x
  20. Imai I., Sunahara T., Nishikawa T., Hori Y., Kondo R., Hiroshi S., 2001, Fluctuations of the red tide flagellates Chattonella spp. (Raphidophyceae) and the algicidal bacterium Cytophaga sp. in the Seto Inland Sea, Japan, Marine biology, 138, 1043-1049 https://doi.org/10.1007/s002270000513
  21. Adachi M., Fukami K., Kondo R, Nishijima T., 2002, Identification of marine algicidal Flavobacterium sp. 5N-3 using multiple probes and whole-cell hybridization, Fish. Sci., 68, 713-720 https://doi.org/10.1046/j.1444-2906.2002.00484.x
  22. 박영태, 박지빈, 정성윤, 송병철, 임월애, 김창훈, 이원재, 1998, 적조생물 살조세균 탐색 I. 유해 적조생물 Cochlodinium polykrikoides 살조세균 Micrococcus sp. LG-1의 분리와 살조특성, 한국수산학회지, 31, 767-773
  23. 정성윤, 박영태, 이원재, 2000, 적조생물 살조세균 탐색, III. 유해성 적조생물 Cochlodinium polykrikoides에 대한 Micrococcus sp. LG-5의 살조효과, 한국수산학회지, 33, 331-338
  24. 정성윤, 박영태, 김무찬, 최석철, 성희경, 김재영, 김채운, 이원재, 2000, 적조생물 살조세균 탐색 IV. 살조세균 Micrococcus sp. LG-5가 생산하는 살조물 질의 특성과 해양생물에 미치는 영향, 한국수산학회지, 33, 339-347
  25. Byun H. G., Jeong S. Y., Park Y. T., Lee W. J., Kim S. K., 2002, Algicidal activity of substance purified from marine bacteria metabolites against Cochlodinium polykrikoides, J. of Fish. Sci. Technol., 5, 150-155
  26. 이원재, 박영태, 1998, 적조생물 살조세균 탐색 II. 적조생물 Prorocentrum micans 살조 세균 Pseudomonas sp. LG-2의 분리와 살조특성, 한국수산학회지, 31, 852-858
  27. Wang X., Gonga L., Liang S., Hana X., Zhua C., Li Y., 2005, Algicidal activity of rhamnolipid biosurfactants produced by Pseudomonas aeruginosa, Harmful Algae, 4, 433-443. https://doi.org/10.1016/j.hal.2004.06.001
  28. Lovejoy C., Bowman J. P., Hallegraeff G. M., 1998, Algicidal effects of a novel marine Pseudoalteromonas isolate (class Proteobacteria, gamma subdivision) on harmful algal bloom species of the genera Chattonella, Gymnodinium, and Heterosigma, Appl. Environ. Microbiol., 64, 2806-2813
  29. Lee S. O., Kato J., Takiguchi N., Kuroda A., Ikeda T., Mitsutani A., Ohtake H., 2000, Involvement of an extracellular protease in algicidal activity of the marine bacterium Pseudoalteromonas sp. strain A28, Appl. Environ. Microbiol., 66, 4334-4339 https://doi.org/10.1128/AEM.66.10.4334-4339.2000
  30. Su J. Q., Yang X. Y., Zheng T. L., Tian Y., Jiao N. Z., Cai L. Z., Hong H. S., 2007, Isolation and characterization of a marine algicidal bacterium against the toxic dinoflagellate Alexandrium tamarense, Harmful Algae, 6, 799-810 https://doi.org/10.1016/j.hal.2007.04.004
  31. Furusawa G., Yoshikawa T., Yasuda A., Sakata T., 2003, Algicidal activity and gliding motility of Saprospira sp. SS98-5, Can.J. Microbiol., 49, 92-100 https://doi.org/10.1139/w03-017
  32. Konkur M., 1986, Gram-positive cocci, In Bergey's Manual of Systematic Bacteriology, Vol. 2, ed. Holts, J. G., Sneath, P. A., Mair, N. S., Sharpe, M. E., The Williams & Wilkins, pp. 1004-1008
  33. MacFaddin T. F., 1984, Biochemical tests for identification of medical bacteria, Williams & Wilkins, pp. 36-308
  34. Wieser M., Denner E. B. M., Kämpfer P., Schumann P., Tindall B., Steiner U., Vybiral D., Lubitz W., Maszenan A. M., Patel B. K. C., Seviour R. J., Radax C., Busse1 H. J., 2002, Emended descriptions of the genus Micrococcus, Micrococcus luteus (Cohn 1872) and Micrococcus lylae (Kloos et al. 1974), Int. J. Syst. Evol. Microbiol., 52, 629-637 https://doi.org/10.1099/00207713-52-2-629
  35. 이창규, 이옥희, 이삼근, 2005, 한국연안에서 분리한 적조형성 미세조류 10종의 성장에 미치는 온도, 염분, 광도의 영향, 한국해양학회지, 10, 79-91
  36. Nakashima T., Miyazaki Y., Matsuyama Y., Muraoka W., Yamaguchi K., Oda T., 2006, Producing mechanism of an algicidal compound against red tide phytoplankton in a marine bacterium proteobacterium, Appl. Microbiol. Biotechnol., 73, 684-690 https://doi.org/10.1007/s00253-006-0507-2
  37. Jeong J. H., Jin H. J., Sohn C. H., Suh K. H., Hong Y. K., 2000, Algicidal activity of the seaweed Corallina pilulifera against red tide microalgae, J. Appl. Phycol., 12, 37-43 https://doi.org/10.1023/A:1008139129057
  38. Lee S. O., Kato J., Nakashima K., Kuroda A., Ikeda T., Takiguchi N., Ohtake H., 2002, Cloning and characterization of extracellular metal protease gene of the algicidal marine bacterium Pseudoalteromonas sp. strain A28, Biosci. Biotechnol. Biochem., 66, 1366-1369 https://doi.org/10.1271/bbb.66.1366
  39. Yoshinaga I., Kawai T., Ishida Y., 1997, Analysis of algicidal ranges of the bacterial killing the marine dinoflagellate Gymnodinium mikimotoi isolated from Tanabe Bay, Wakayama Pref., Japan, Fish. Sci., 63, 94-98 https://doi.org/10.2331/fishsci.63.94