• 한국식품조리과학회지
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• 제28권1호
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• pp.41-49
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• 2012

This purpose of this study was to develop a tea with small red bean which have been known to have effect regarding anti-obesity, fatigue recovery, edema recovery, blood circulation, etc. In order to provide baseline data for small red bean tea we investigated the general components, antioxidative effect and proanthocyanidin analysis in small red beans[Phaseolus angularis W.F. Wight.]. Physicochemical analysis(pH, sugar content, salinity, turbidity), color, anti oralmicrobial activity, content of saponin and sensory test of small red bean with different boiling time in 8 minutes(SR1), 16 minutes(SR2), 24 minutes(SR3), 32 minutes(SR4), 40 minutes(SR5) was also measured. It was shown that the crude fat, carbohydrate, moisture, crude protein, crude ash content of small red bean were 1.0%, 63.9%, 12.8%, 18.7%, 3.6%. DPPH free radical scavenging activity, the total phenolic compounds content and flavonoid content increased significantly (p<0.001). The results of analyzed proanthocyanidin was distinguished by characteristic UV-visible spectra with absorption maximum at 320 nm($t_R$ 7.589 min). As the boiling time(8 minutes:SR1, 16 minutes:SR2, 24 minutes:SR3, 32 minutes:SR4, 40 minutes:SR5) of small red beans increaseds, the pH significantly decreased(p<0.001). The sugar content, salinity and turbidity significantly increased(p<0.001). Moreover, Hunter L, a and b values, crude saponin also increased(p<0.001). The results of analyzed activity against oral bacteria, S. mutans, S. sobrinus, P. intermedia and P. gingivalis showed a higher antibacterial activities than E. coli and S. aureus. MIC was measured that S. mutans, S. sobrinus, P. intermedia and P. gingivalis showed a lower MICs than E. coli and S. aureus. The results regarding sensory test measures, In case of color, refreshing taste and overall quality, SR3 had the highest preference overall among tested samples. In cases conceming odor and taste, SR5 had the highest preference and with regards to sweetness and saltyness, SR4 had the highest preference.

• Tuberculosis and Respiratory Diseases
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• 제53권6호
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• pp.662-672
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• 2002

Amiodarone 폐독성은 약제 복용력이 있는 환자에서 새로운 증상과 X-선의 변화가 있을 때 의심하여야 하며 울혈성 심부전이나 폐감염증, 폐색전증, 악성종양 등과 감별하는 것이 중요하다. 진단은 임상적, 방사선학적, 조직학적 소견을 종합하여 내릴 수 있으며 대부분의 경우에는 약제 중단을 통해서 폐독성의 호전을 기대할 수 있으나 일부에서는 호흡부전으로 진행하거나 기존의 심부정맥의 재발 등으로 인하여 사망하는 경우도 있다. 저자들은 심부정맥으로 amiodarone을 사용하던 환자에서 치명적인 폐독성이 발생한 4예를 경험하였기에 이를 문헌고찰과 함께 보고하는 바이다.

• 보건교육건강증진학회지
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• 제13권1호
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• pp.90-116
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• 1996

The purpose of this study is to provide basic data for the children so as to avoid any accidents and to improve their health through a sound and safe living environment with safety education established through more systematic methods. For the study, the methods used include the questionnaires for collecting data which are distributed among 273 children from 3 to 6 years old. They live in three districts of Seoul (Kangnam and Kangbuk), Kyeonggido (Munsan and Ilsan) and Incheon. The children's general characteristics are investigated and the relationship between the accident-proneness prospects and their results are analyzed assuming that children's health is expected to have an effect on their safety. The findings of the study are as follows: 1. The accident-proneness prospect (for the living safety) has significant difference in sleeping habits and physically weak conditions(p<0.05). 2. The accident-proneness prospect (for the traffic safety) has no significant difference in accordance with the children's health condition. 3. Children's reasoning power has no significant difference in accordance with their health condition. 4. Children's movement speed non-significantly differs in accordance with their health condition. 5. Children's movement stability has a significant difference in accordance with their health conditions such as history of mild disease(p<0.05) and physical work capacity(p<0.05). 6. Children's attention power has no significant difference in accordance with their health condition. 7. Children's characteristic activities show a significant difference in accordance with their health history of mild disease(p<0.05). mental health(p<0.05). eating habits(p<0.05) and others (i.e. auditory abnormality and skin desease)(p<0.05). 8. General assessment of the APP test has no significant difference in accordance with their health condition. 9. The health conditions are a significant difference in the history of mild disease in accordance with sex(p<0.05) and whole family members(p<0.001). Also there are shown significant differences. in accordance with sex(p<0.05). in physical work capacity(p<0.05). Eye sight, blood circulation and skin condition show significant differences in accordance with residence(p<0.05) and whole family members(p<0.01). and the condition of eating habits appeared significant in accordance with age(p<0.05). Also, sleeping habits and physically weak conditions shows significant differences in accordance with age(p<0.001) and housing pattern(p<0.01), and others (i.e. auditory abnormality and skin desease) show significant differences in accordance with residence (p<0.001).

• 한국양식학회지
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• 제9권1호
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• pp.83-92
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• 1996

농어목 어류, C. diagramma 암컷의 vitellogenin과 난황단백의 면역화학적 특성을 서로 비교하였다. 면역화학적 방법에 의해 C. diagramma 암컷의 혈청에서 vitellogenin 1 (VTG1)과 vitellogenin 2 (VTG2)를 동정하였으며, sephacryl S-300 gel 여과법에 의하여 측정된 분자량은 각각 560,000과 410,000이었다. 암컷의 난소 추출액에서는 두 가지 형태의 난황단백인 E2와 E3가 분리되었으며, 이들의 분자량은 각각 410,000과 170,000이었다. $17\beta$-estradiol ($E_2$)을 주사한 수컷의 혈청과 간조직을 abidine-biotin complex (ABC) 방법으로 분석한 결과, vitellogenin과 유사한 물질이 검출되었다. 이상의 결과로 부터, 외인성 $E_2$ 에 의해 간에서 합성된 vitellogenin은 혈액 속으로 방출된 후 난소로 이동되는 것으로 추정되었다. 암컷의 $E_2$, 혈중농도는 산란기인 6월에 최고치를 나타내었고, vitellogenin 상대농도도 $5\~6$월에 최고치를 나타내었다. $E_2$와 vitellogenin의 변화는 본 종의 난소성숙과 밀접한 관계가 있으며, 난황형성에 있어서도 중요한 역할을 하고 있음을 알 수 있었다. 암컷 혈청 VTG2와 난소추출액 E2의 분자량이 서로 같고, vitellogenin이 간에서 합성되어 난소내로 이동하는 점으로 보아, 난소내 난황단백 성분의 전구물질은 혈청내의 vitellogenin인 것으로 추정되었다.

• 대한한의학회지
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• 제24권2호
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• pp.166-178
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• 2003

Objectives : Nitric oxide (NO) plays an important role in normal and pathophysiological cells as a messenger molecule, neurotransmitter, microbiological agent, or dilator of blood vessels and arteriosclerosis, respectively. This study was undertaken to understand the mechanism of NO production and effect of Hyeolbuchukeo-tang (Xiefuzhuyu-tang) on NO production in cultured vascular smooth muscle cell (VSMC). Methods and Results : VSMC was isolated from aorta and cultured. Cultured primary cells were identified as VSMC with anti--smooth muscle actin antibody. A large amount of NO was produced in cultured VSMC treated with $IFN-{\gamma}$ plus TNF in a time- and dose-dependent manner. $TNF-{\alpha}$ was a more efficient stimulator than $IFN-{\gamma}$ in NO production of cultured VSMC. iNOS protein wasdetected within 3 hrs and it increased up to 12 hrs in a time-dependent manner. However, accumulated NO in cytokine-treated VSMC was not detected within 3 hrs. NO production in cytokine-treated VSMC showed the dose- and time-dependent manner, and increased up to 48 hrs. The activated VSMC produced a large amount of NO (about 60 uM). Hyeolbuchukeo-tang (Xiefuzhuyu-tang) alone did not induceNO production, but it potentiated the effect of $TNF-{\alpha}$ on NO production and increased NO production by about 20%. Hyeolbuchukeo-tang (Xiefuzhuyu-tang) did not affect the transcriptional activity of iNOS gene, but increased the accumulation of iNOS. These results indicate that Hyeolbuchukeo-tang (Xiefuzhuyu-tang) could modulate the translational level of iNOS. PKC did not modulate NO production, but calcium ionophore A23187 decreased NO production. However, Hyeolbuchukeo-tang (Xiefuzhuyu-tang) elevated the decreased NO production in A23187-treated VSMC by modulating the stability of iNOS transcripts. Half-life of the synthesized transcripts appeared to have about 6 hrs. PDTC, an $NF-{\kappa}B$ inhibitor, blocked the accumulation of iNOS mRNA, indicating that $NF-{\kappa}B$ served as an important modulator in the transcriptional regulation of iNOS. As Hyeolbuchukeo-tang (Xiefuzhuyu-tang) potentiated the effect of the $TNF-{\alpha}$ on NO production but had no additional effect on PDTC-modulated NO production, it is suggested that Hyeolbuchukeo-tang (Xiefuzhuyu-tang) enhances the $TNF-{\alpha}-mediated$ NO production of VSMC by modulating the iNOS activity and the stability of iNOS transcripts in activated VSMC having the elevated intracellular calcium ion. Conclusions : This study suggests that Hyeolbuchukeo-tang (Xiefuzhuyu-tang) has a potential capacity for preventing and treating diseases of the circulation system, including arteriosclerosis.

• 한국응용과학기술학회지
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• 제37권5호
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• pp.1239-1247
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• 2020

한국 자생식물인 기린초(Sedum kamtschaticum Fisch.)는 열수 추출 형태로 혈액 순환 개선과 항산화 및 항염증 효과를 내는 한약재로 사용되어왔다. 선행연구를 통해 기린초 여러 부위에서 우수한 항산화 효과를 확인하여, 본 연구에서는 기린초의 줄기(Stem)와 뿌리(Root) 부위를 각각 70% ethanol (SKS, SKR)로 추출한 후, n-hexane (SSH), ethyl acetate (SSE, SRE), chloroform (SSC, SRC) 및 water (SSW, SRW) 순서로 용매 극성별로 분획하여 화장품 소재로서 응용 가능성을 확인하였다. 각 분획물마다 총 폴리페놀 함량, 플라보노이드 함량 및 DPPH 라디칼 소거능을 확인한 결과 뿌리추출물(SKR)이 줄기추출물(SKS)보다 우수한 함량과 효과를 나타냈고, 전체적으로 ethyl acetate 분획물(SSE, SRE)이 가장 효과가 우수했다. 또한, tyrosinase 활성 저해 효과도 ethyl acetate 분획물이 가장 우수하였고, 모든 분획물은 세포독성이 없는 10 ㎍/mL 농도에서 B16F10 멜라노마 세포에 처리했을 때 우수한 멜라닌 생성 저해력을 보였다. 70% ethanol 추출물(SKS, SKR)에 대해 HPLC 분석 시 gallic acid와 quercetin을 포함한 플라보노이드가 검출되었다. 본 연구에서는 기린초의 피부 항산화 효과와 미백효과를 나타내는 기능성화장품 천연소재로서 응용 가능성을 제시한다.

• 한국식품영양과학회지
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• 제32권6호
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• pp.906-912
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• 2003

4주간의 고지혈 유도식이를 섭취시킨 후 고지혈증 유도가 이루어진 실험 동물을 이용하여 생식 섭취로 인한 고지혈증 개선 효과를 확인하고자 하였다. 고지혈 유도가 확인된 실험 동물에게 고지혈사료에 30% 생식을 혼합한 식이를 투여하였을 경우 혈중 콜레스테롤량을 약 37% 정도 감소시킨 것으로 나타났으며, 간조직중의 콜레스테롤 역시 약 28% 감소한 것으로 나타나 생식의 섭취가 체내의 콜레스테롤 농도를 낮추어 주는 효과가 있는 것으로 확인되었다. 또한, 체내 콜레스테롤 구성에 있어서도 관상동맥 질환을 억제하는 것으로 알려진 HDL-C의 함량은 대조군에 대비하여 생식 섭취군이 약 28%정도 증가되었으며, 동맥벽에 축적되어 관상동맥질환 발병과 연관관계가 높은 것으로 알려진 LDL-C의 함량을 약 39%정도 감소시킨 것으로 나타나 체내 콜레스테롤 수치의 저하와 더불어 콜레스테롤의 구성 양상에도 이로운 점을 제공한 것으로 나타났다. 이러한 결과로부터 순환기계 질환 발병율을 나타내는 지표로 널리 이용되는 동맥경화지수와 심혈관위험지수를 산출한 결과 생식 섭취군은 대조군에 대비하여 동맥경화지수의 약 60%를 저하시켰으며, 심혈관위험지수 역시 약 56% 정도 저하시킨 것으로 나타났다. 이상의 결과를 종합하여 볼 때 생식 섭취는 체내 콜레스테롤의 농도를 저하시킴으로서 순환기계 질환의 위험도를 낮추는데 효과가 있는 것으로 생각된다.

• 동의생리병리학회지
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• 제19권5호
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• pp.1169-1180
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• 2005

First, I read all the materials, including Dongeuibogam(Encyclopedia Medica Koreana), classical writings on Lacca sinica exsiccata, herbal writings on lacquer poison, and herbal books on how to treat lacquer poison. And then after 1 examined all the details on qi and taste of Lacca sinica exsiccata, its efficacy, detoxification, lacquer poison, and its effects on body symptom, 1 got the following results. The order of frequency that Lacca sinica exsiccata is used in Dongeuibogam is pressure-feeling, blood circulation, and insect biting. Its way of intake is not so much through herb-boiling or powdered medicine as through hand-made pills. When medicine is used in the form of pills, the Lacca sinica exsiccata is more included among other ingredients. When old doctors treated pressure-feeling in the chest, they mixed up other herbs, with not putting more emphasis on the efficacy of lacquer 010 doctors believed that toxicity of Lacca sinica exsiccata is not having its own poison, but having biased dominance in the use of its qE and taste. The way or detoxification or Lacca sinica exsiccata is used in the order of crab-boiled water, egg, Xanthoxylum piperitum, Perilla frutescens, Astar tataricus, a weeping willow, iron-tempered water, and Allium toberosum. Special point in detoxificating lacquer poison is that they used medicines for well-ciruculating pulmonary stream, medicines for promoting to urinate or discharge by helping large colons to move, medicines for making the lacquer scar small, medicines for helping digest, and medicines for improving vessel function in the poisoned area. With the above results, the more profound study, based on the crab-boiled water and egg, is expected to go on to increase the effect on the one hand, and to make the new way of lessening or removing the toxicity of lacquer with more safe use on the other hand.

• 동의생리병리학회지
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• 제22권2호
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• pp.357-364
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• 2008

Mylabris is the dried body of the chinese blister beetle. The species used in medicine are Mylabris phalerata and M. cichorii. In recent studies, it has been found that Mylabris possesses antitumor properties, increases the number of leukocytes, and has irritant effects on the urinary organs. The crude extracts of Mylabris have been noted for their highly irritant action and other traditional uses of Mylabris include treatment of poor local blood circulation. The active constituent of Mylabris is cantharidin. The chemical is notable for its vesicant properties, but with severe side effects such as nephrotoxicity. This experiment examined the effect of extracts and fractions, obtained from Mylabris phalerata Pall. on hair growth activity of the C57BL/6N mice after topical application to skin. First, we examined the effect of an extracts, obtained from the alcohol extracts of dried Mylabris phalerata Pall. on hair growth activity of the C57BL/6N mice after topical application to skin. Second, we examined on hair growth activity of the cantharidin fraction of Mylabris phalerata Pall. compared to the control and 1% minoxidil groups. Third, we investigated the number of hair follicle and mast cells after topical application of extracts of Mylabris phalerata Pall. to skin for 16 days. The results were as follows: Hair growth effect from the extracts of Mylabris phalerata Pall.(0.312%) was observed in 80% of mice whose hair had been removed in 13 days. Hair growth effect from the extract of Mylabris phalerata Pall.(0.312 and 0.625%) and 1% minoxidil group was observed in 100% of mice whose hair had been clipped in 20 days. Hair growth effect from the cantharidin fraction(0.5%) and water fraction(0.5%) of Mylabris phalerata Pall. was observed in 100% of mice whose hair had been clipped in 24 days. The hair growth effect on the cantharidin fraction(0.125%) was observed to be strong compared with the minoxidil(3%) group, commercial hair growth agents, in mice whose hair had been clipped in 19 days. In the spontaneous alopecia mice model, the hair growth effect from the cantharidin fraction (0.125%) was observed to be strong as compared with the states before the 13 days experiment. These experiments suggest that extracts and fractions of Mylabris phalerata Pall. may stimulate the topical hair growth activity in low doses.

• Neonatal Medicine
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• 제17권1호
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• pp.109-115
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• 2010

신생아의 지속성 폐동맥 고혈압증(PPHN)은 출생 후 폐동맥의 저항이 낮아지고 전신 혈관 저항이 올라가면서 폐로 가는 혈류가 증가하고 난원공과 동맥관을 통한 폐와 전신의 평행 순환에서 연속 순환인 신생아 순환으로 바뀌게 되는 과정에 문제가 있는 것이다. 저자들은 태변흡인이 있었던 두 명의 만삭아에서 고빈도 환기 요법과 흡인성 일산화질소 가스를 이용하여 PPHN을 치료하였으나 치료에 반응하지 않고 전신 장기에 산소화가 문제가되어 정맥-정맥도관체외막형산소섭취(V-V ECMO)를 시행하였고 두 증례 모두 체외막형 산소 섭취를 이탈하여 생존할 수 있었다. 현재까지 태변 흡인 증후군에 의해 생긴 PPHN 환아들에서 ECMO를 적용하여 치료한 국내 보고가 없고, 특히 V-V ECMO 적용으로 신생아를 치료한 보고 또한 없다. 이에 저자들은 태변 흡입 증후군에 의해 생긴 PPHN 환아들에서 V-V ECMO로 생존한 2례를 경험하였기에 문헌 고찰과 함께 보고하는 바이다.