Browse > Article

EXperimental Studies on the Hair Growth Activity of Mylabris Phalerata Pall. Extracts and Fractions in C57BL/6N Mice  

Jo, Han-Young (Department of Anatomy, College of Oriental Medicine, Woosuk University)
Kim, Tae-Heon (Department of Anatomy, College of Oriental Medicine, Woosuk University)
Kim, Ho (Department of Pathology, College of Oriental Medicine, Woosuk University)
Jeong, Han-Sol (Department of Pathology, College of Oriental Medicine, Woosuk University)
Lee, Chang-Hyun (Department of Anatomy, College of Oriental Medicine, Woosuk University)
Lee, Gwang-Gyu (Department of Pathology, College of Oriental Medicine, Woosuk University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.22, no.2, 2008 , pp. 357-364 More about this Journal
Abstract
Mylabris is the dried body of the chinese blister beetle. The species used in medicine are Mylabris phalerata and M. cichorii. In recent studies, it has been found that Mylabris possesses antitumor properties, increases the number of leukocytes, and has irritant effects on the urinary organs. The crude extracts of Mylabris have been noted for their highly irritant action and other traditional uses of Mylabris include treatment of poor local blood circulation. The active constituent of Mylabris is cantharidin. The chemical is notable for its vesicant properties, but with severe side effects such as nephrotoxicity. This experiment examined the effect of extracts and fractions, obtained from Mylabris phalerata Pall. on hair growth activity of the C57BL/6N mice after topical application to skin. First, we examined the effect of an extracts, obtained from the alcohol extracts of dried Mylabris phalerata Pall. on hair growth activity of the C57BL/6N mice after topical application to skin. Second, we examined on hair growth activity of the cantharidin fraction of Mylabris phalerata Pall. compared to the control and 1% minoxidil groups. Third, we investigated the number of hair follicle and mast cells after topical application of extracts of Mylabris phalerata Pall. to skin for 16 days. The results were as follows: Hair growth effect from the extracts of Mylabris phalerata Pall.(0.312%) was observed in 80% of mice whose hair had been removed in 13 days. Hair growth effect from the extract of Mylabris phalerata Pall.(0.312 and 0.625%) and 1% minoxidil group was observed in 100% of mice whose hair had been clipped in 20 days. Hair growth effect from the cantharidin fraction(0.5%) and water fraction(0.5%) of Mylabris phalerata Pall. was observed in 100% of mice whose hair had been clipped in 24 days. The hair growth effect on the cantharidin fraction(0.125%) was observed to be strong compared with the minoxidil(3%) group, commercial hair growth agents, in mice whose hair had been clipped in 19 days. In the spontaneous alopecia mice model, the hair growth effect from the cantharidin fraction (0.125%) was observed to be strong as compared with the states before the 13 days experiment. These experiments suggest that extracts and fractions of Mylabris phalerata Pall. may stimulate the topical hair growth activity in low doses.
Keywords
Mylabris phalerata Pall.; C57BL/6N mice; hair growth activity; cantharidin; minoxidil;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 中藥大辭典編纂委員會. 完譯中藥大辭典. 鼎談, 第9卷, pp 1486- 1490, 1997
2 Xu, B. Pharmacology of some natural products of china. trends Pharmacol Sci 271: 272, 1981
3 Wang, C.C., Wu, C.H., Hsieh, K.J., Yen, K.Y., Yang, L.L. Cytotoxic effects of cantharidin on the growth of normal and carcinoma cells. Toxicology 147: 77-87, 2000   DOI   ScienceOn
4 Harry, R.G. Hair tonics. In: Harry, R.G., editor. Harrys cosmeticology. London, George-Godwin Ltd, pp 426-436. 1976
5 李元文, 張韋川. 名醫臨證經驗. 北京,人民衛生出版社, p 572,2001
6 孫思邈. 備急千金要方. 人民衛生出版社. p 297, 1982
7 陳實功. 外科正宗. (中華經典醫書 第六集), 中國醫藥科學技術出版社, p 987, 2002
8 임사비나, 최규동, 김수경. 탈모에 대한 동서의학적 고찰. 경산대학교 제한동의학술원 논문집 4(1):699-710, 1999
9 노현찬, 노석선. 苦蔘추출물이 모발성장 촉진 및 면포억제에 미치는 영향. 대한안이비인후피부과학회지 15(1):96-126, 2002
10 Bergfeld, W. Diffuse hair loss in women. Cutis 22: 190-195, 1987
11 吳惠時, 江小靑. 斑禿攃劑的硏制及臨床應用. 基層中葯雜誌 13: 57, 2000
12 Argyris, T.S. Kinetics of epidermal production during epidermal regeneration following abrasion in mice. Am J Pathol 83: 329-340, 1956
13 熊霞, 振德宇, 呂曉雅. 斑禿患者血液流變學的變化. 中國皮膚性病學雜誌 14(5):313-314, 2000
14 姜義娜, 斑禿藥物治療的新進展. 中國皮膚性病學雜誌 17: 203- 204, 2003
15 鄭虎占, 董澤宏, 余靖. 中藥現代硏究與應用. 學苑出版社, 제4권, pp 3328-3344, 1998
16 李林. 實用中醫皮膚病學. 北京, 中醫古籍出版社, p 60, 61, 1998
17 裵正學, 侯瑞云, 傳秀琴. 辨證治療斑禿和早禿73例初步報告. 中醫雜誌 28: 43-44, 1987
18 Selmanowilz, V., Victor, S., Warburton, D., Orentreich, N. Fingerprints arches in alopecia areata. Arch. Dermatol. 110: 570-571, 1974   DOI   ScienceOn
19 Stenn, K.S., Paus, R. Controls of hair follicle. Physiological Reviews 81: 449-494, 2001   DOI
20 Silverberg, N.B., Sidbury, R., Mancini, A.J. Childhood molluscum contagiosum:Experience with cantharidin thera- phy in 300 patients. J Am Acad Dermatol 43: 503-507, 2000   DOI   ScienceOn
21 Toback, C., Ajkuna,r S. The emotional disturbances underlying alopecia areata, alopecia totalis and trichotillomania. Child Psychiatry Hum Dev 10: 114-117, 1979   DOI   ScienceOn
22 朱仁康. 脫髮證治. 中醫雜誌 2(12):46, 1986
23 송순기, 이명종. 안드로겐성 탈모증의 두피재활에 관한 문헌적 고찰. 한의정보학회지 5(1):162-176, 1999
24 Silver, A.F., Chase, H.B., Arsenault, C.T. Early anagen initiated by plucking compared with early spontaneous anagen. Adv Biol Skin 9: 265-286, 1969
25 Friedman, P.S. Alopecia areata and auto-immunity. Br J Dermatol 105: 153-157, 1981   DOI   ScienceOn
26 斳毅, 吳玉山, 溫秀珍, 李堃. 外用中葯酒治療斑禿8例臨床觀察. 中醫外治雜誌 13(5):9, 2004
27 葛正義. 血液流變學輿甲皺微循環檢測對脂漏性脫髮中醫分型及治療的臨床意義. 淅江中西醫結合雜紙 21: 107, 1998
28 黃泰康, 李春頭, 虫類本草. 中國醫藥科技出版社, pp 66-71, 2002.
29 Argyris, T.S. The effects of wounds on adjacent growing or resting hair follicles in mice. Arch Dermatol Symp 61: 31-36, 1956
30 徐建榮, 朱拉珍, 陳凌風. 步長腦心通聯合銀杏葉片治療斑禿前後甲襞微循環觀察. 河北中醫 22(1):42, 2000
31 張和平. 斑蝥液治療斑禿. 中醫外治雜誌 7: 5, 1997
32 Muller, H.K., Rook, A.J., Kubba, R. Immunohistology and autoantibody studies in alopecia areata. Br. J. Dermatol. 102: 609-610, 1980   DOI   ScienceOn
33 黃帝內經注解補注素問. 素問.五藏生成論. 金達鎬, 醫聖堂, p 236, 2001
34 Lynfield, Y.L., MAC Williams, P. Shaving and hair growth. J Invest Dermatol 55: 170-172, 1970   DOI   ScienceOn
35 葛洪. 肘後備急方. 中國醫學大系 734권, 驪江出版社, p 500, 1995
36 Walter, W.G., Cole, F. Isolation of cantharidin from Epicauta pestifera. J Pharm Sci 56: 174-176, 1967   DOI
37 Polettini, A., Crippa, O., Ravagli, A., Saragoni, A. A fatal case of poisioning with cantharidin. Forensic Sci Int 55: 37-43, 1992   DOI   ScienceOn
38 石豔茹, 王利紅, 斑蝥臨床新用种种. 內蒙古中醫葯, 18: 82, 1999
39 Hundt, H.K., Styeyn, J.M., Wagner, L. Postmortem serum concentration of cantharidin in a fatal case of cantharidin poisioning. Hum Exp Toxicol 9: 35-40, 1990   DOI   ScienceOn
40 黃帝內經注解補注素問. 素問.上古天眞論. 金達鎬, 醫聖堂, p 18, 2001
41 丁光迪. 諸病源候論校注. 人民衛生出版社, p 763-772, 1991
42 Robson, H.J., Cunningham, N.M., Kruzan, K.L., Patel, D.S., Kreiter, C.D., O,Donnell, M.J., Arpey, C.J. Pulsed-dye laser versus conventional theraphy in the treatment of warts: A prospective randomized trial. J Am Acad Dermatol 43: 275-280, 2000   DOI   ScienceOn
43 王本祥, 現代中葯葯理與臨床. 天津科技翻譯出版公司, pp 1483- 1487, 2004
44 黃帝內經注解補注素問. 素問.五藏生成論. 金達鎬, 醫聖堂, p 236, 2001
45 吳謙. 醫宗金鑑(下卷). 人民衛生出版社, p 1660, 1982
46 Bensky, D., Gamble, A. Chinese herbal medicine, Materia Medica, Eastland press, p 457, 1986
47 黃帝內經注解補注素問. 素問.上古天眞論. 金達鎬, 醫聖堂, p 14, 2001
48 홍석훈, 노석선. 모발탈락의 병인 및 치료법에 대한 문헌적 고찰. 외관과학회지 9(1):43-65, 1996
49 김소영, 김중호, 채병윤. 탈모에 응용되는 神應養眞丹의 효능에 관한 실험적 연구. 외관과학회지 4(1):43-58, 1991
50 葉子雨. 難經正義. (珍本醫書集成 1卷). 醫聖堂, p 284, 1994