• KSII Transactions on Internet and Information Systems (TIIS)
• /
• 제12권4호
• /
• pp.1436-1457
• /
• 2018

Secure routing services in Wireless Sensor Networks (WSNs) are essential, especially in mission critical fields such as the military and in medical applications. Additionally, they play a vital role in the current and future Internet of Things (IoT) services. Lightness and efficiency of a routing protocol are not the only requirements that guarantee success; security assurance also needs to be enforced. This paper proposes a Secure-Fuzzy Energy Aware Routing Protocol (S-FEAR) for WSNs. S-FEAR applies a security model to an existing energy efficient FEAR protocol. As part of this research, the S-FEAR protocol has been analyzed in terms of the communication and processing costs associated with building and applying this model, regardless of the security techniques used. Moreover, the Qualnet network simulator was used to implement both FEAR and S-FEAR after carefully selecting the following security techniques to achieve both authentication and data integrity: the Cipher Block Chaining-Message Authentication Code (CBC-MAC) and the Elliptic Curve Digital Signature Algorithm (ECDSA). The performance of both protocols was assessed in terms of complexity and energy consumption. The results reveal that achieving authentication and data integrity successfully excluded all attackers from the network topology regardless of the percentage of attackers. Consequently, the constructed topology is secure and thus, safe data transmission over the network is ensured. Simulation results show that using CBC-MAC for example, costs 0.00064% of network energy while ECDSA costs about 0.0091%. On the other hand, attacks cost the network about 4.7 times the cost of applying these techniques.

• 한국융합학회논문지
• /
• 제6권1호
• /
• pp.23-28
• /
• 2015

프로그래밍 교육은 컴퓨터 관련학과 뿐만 아니라 공학과 자연과학분야에 걸쳐서 모두 요구되고 있다. 더욱이 최근에는 초등학교와 중등학교에서도 소프트웨어 교육의 중요한 부분으로 프로그래밍 교육이 강조되고 있다. 프로그래밍 능력은 국가의 경쟁력을 이루는 필수적인 요소로 이해되고 있어, 이를 위한 학습시스템이 요구되고 있다. 본 논문에서는 구글에서 개발한 Blockly graphic 툴을 이용해서 웹상에서 실행하는 webc 프로그래밍 융합 학습시스템을 구현하였다. 또 학습시스템 안에는 문제 중심의 학습에 이용할 수 있는 초보자용 문제세트가 내장되어 있다. 이 문제세트는 20여년 동안 현장에서 검증받은 문제들로 학습자들이 최단 시간 내에 초보를 탈출할 수 있게 해주는 문제세트 들이다. Blockly webc 프로그래밍 융합 학습시스템은 이미 개발된 Simple Visual Language2 프로그래밍 학습시스템과 함께 초보자를 위한 프로그래밍 학습시스템으로 중요한 역할을 할 것으로 기대된다.

• 방송공학회논문지
• /
• 제9권3호
• /
• pp.225-235
• /
• 2004

본 논문에서는 이질적인 수신 단말을 위해 시공간적 계위(spatio-temporal scalability)를 이용한 새로운 3차원 영상 부호화 방법을 제안한다. 제안된 방법은 좌 영상의 부호화를 위해 MPEG-2 표준을 이용하며, 우 영상의 부호화를 위해서 향상된 호환성(enhanced compatible) 부호화 방법을 이용하여 P-와 B-타입의 영상을 예측한다. 향상된 호환성 부호화 방법은 두 개의 전후방향 움직임으로부터 예측된 매크로 블록과 양안차로부터 예측된 블록을 보간하여 매칭 블록을 예측한다. 유연성 있는 3차원 비디오 서비스를 위해서, 각각의 좌우 영상에 대해 시공간적 확장 가능한 계층을 정의하였다. 실험을 통하여 제안된 3차원 영상 부호화기의 성능을 기존의 방법들과 비교하여 검증하였으며, 양안차 예측의 이점을 계위에 따르는 오버헤드의 관점에서 분석하였다. 제안된 시공간적 계위를 이용한 3차원 영상 부호화기는 다양한 디스플레이 장치 및 네트워크 환경을 가지는 유비쿼터스 컴퓨팅 환경을 위한 효율적인 3차원 비디오 전송 서비스에 활용될 것으로 기대된다.

• 한국환경보건학회지
• /
• 제40권1호
• /
• pp.17-26
• /
• 2014

Background: Despite children's unique characteristics that distinguish them from adults, relatively few attempts have been made to measure exposure factors for characterization of children's exposure to hazardous chemicals in child-specific products (CSP). This study was conducted to establish the child-specific exposure factors for exposure and risk assessment of hazardous substances in CSP. Methods: We investigated the exposure factors (e.g., time use of child-products, time and frequency of object-to-body contact, time and frequency of object-to-mouth contact) influencing children's exposure to CSP (e.g., toys, playmats, oil pastels, etc.) in 650 children through a parent-completed questionnaire using a web-based survey. Participants were recruited in five age groups, <1, 1-2, 2-3, 3-6, and 6-12 years of age. Results: The child-specific exposure factors were presented as the mean, median, $95^{th}$ percentile, minimum, and maximum values. Time activity for play mats was the longest among CSP and infants spent more time on them than did elder age groups (189.3-224.7 min/day for <1-2 years vs. 91.2 min/day for 6-12 years). It is apparent that time and frequency of toy block- and plastic toy-to-mouth contact significantly decreased as a function of age. When the variation of CSP use patterns was compared by gender, the only variable that was statistically different between genders was time activity in child-products exposure space. Conclusion: We believe the five child-specific exposure factors suggested in the present study will be valuable for reducing uncertainty in the estimation of chemical exposure during risk assessment of CSP and furthermore, in the appropriate regulations to protect children's health.

• 한국건설관리학회:학술대회논문집
• /
• 한국건설관리학회 2003년도 학술대회지
• /
• pp.258-261
• /
• 2003

해체공사는 신축공사보다 위험하며 환경위해요인을 많이 야기시킨다. 그러나 해체공사현장에서 사용되는 가설울타리는 환경위해요인의 차단, 안정성과 작업공간확보의 역할을 하지 못한다. 단지 외부의 시선을 차단한 뿐이다. 이 연구는 가설울타리가 해체공사 초기에 설치될 수 있다는 가능성에서 시작하였다. 연구의 초기단계로 실제 가설울타리가 사용되는 실태를 파악하였으며 그 결과 가설울타리가 해체공사 이전에 설치할 수 있음을 알게 되었다. 이에 우리는 해체공사와 신축공사용 가설울타리의 통합을 실제화하는 데 주력하였다. 연구에 결과는 다음과 같다. .첫째, 통합에 제한요소를 파악해야 한다. 둘째, 건축공사 표준시방서의 보완이 필요하다. 셋째, 가설울타리의 훼손 및 변경에 대비해야한다. 마지막으로 가설울타리의 통한을 통해 환경위해요인 저감, 공사비 절감, 작업환경 개선, 해체공사의 이미지 제고를 기대할 수 있다.

• The Korean Journal of Physiology and Pharmacology
• /
• 제20권2호
• /
• pp.193-200
• /
• 2016

Sertraline, a selective serotonin reuptake inhibitor (SSRI), has been reported to lead to cardiac toxicity even at therapeutic doses including sudden cardiac death and ventricular arrhythmia. And in a SSRI-independent manner, sertraline has been known to inhibit various voltage-dependent channels, which play an important role in regulation of cardiovascular system. In the present study, we investigated the action of sertraline on Kv1.5, which is one of cardiac ion channels. The effect of sertraline on the cloned neuronal rat Kv1.5 channels stably expressed in Chinese hamster ovary cells was investigated using the whole-cell patch-clamp technique. Sertraline reduced Kv1.5 whole-cell currents in a reversible concentration-dependent manner, with an $IC_{50}$ value and a Hill coefficient of $0.71{\mu}M$ and 1.29, respectively. Sertraline accelerated the decay rate of inactivation of Kv1.5 currents without modifying the kinetics of current activation. The inhibition increased steeply between -20 and 0 mV, which corresponded with the voltage range for channel opening. In the voltage range positive to +10 mV, inhibition displayed a weak voltage dependence, consistent with an electrical distance ${\delta}$ of 0.16. Sertraline slowed the deactivation time course, resulting in a tail crossover phenomenon when the tail currents, recorded in the presence and absence of sertraline, were superimposed. Inhibition of Kv1.5 by sertraline was use-dependent. The present results suggest that sertraline acts on Kv1.5 currents as an open-channel blocker.

• The Korean Journal of Physiology and Pharmacology
• /
• 제16권5호
• /
• pp.343-348
• /
• 2012

Blocking or regulating $K^+$ channels is important for investigating neuronal functions in mammalian brains, because voltage-dependent $K^+$ channels (Kv channels) play roles to regulate membrane excitabilities for synaptic and somatic processings in neurons. Although a number of toxins and chemicals are useful to change gating properties of Kv channels, specific effects of each toxin on a particular Kv subunit have not been sufficiently demonstrated in neurons yet. In this study, we tested electro-physiologically if heteropodatoxin2 ($HpTX_2$), known as one of Kv4-specific toxins, might be effective on various $K^+$ outward currents in CA1 neurons of organotypic hippocampal slices of rats. Using a nucleated-patch technique and a pre-pulse protocol in voltage-clamp mode, total $K^+$ outward currents recorded in the soma of CA1 neurons were separated into two components, transient and sustained currents. The extracellular application of $HpTX_2$ weakly but significantly reduced transient currents. However, when $HpTX_2$ was added to internal solution, the significant reduction of amplitudes were observed in sustained currents but not in transient currents. This indicates the non-specificity of $HpTX_2$ effects on Kv4 family. Compared with the effect of cytosolic 4-AP to block transient currents, it is possible that cytosolic $HpTX_2$ is pharmacologically specific to sustained currents in CA1 neurons. These results suggest that distinctive actions of $HpTX_2$ inside and outside of neurons are very efficient to selectively reduce specific $K^+$ outward currents.

• The Korean Journal of Physiology and Pharmacology
• /
• 제2권5호
• /
• pp.611-616
• /
• 1998

Although the $Ca^{2+}-activated\;K^+\;(I_{K,Ca})$ channel is known to play an important role in the maintenance of resting membrane potential, the regulation of the channel in physiological condition is not completely understood in vascular myocytes. In this study, we investigated the role of cytoplasmic $Mg^{2+}$ on the regulation of $I_{K,Ca}$ channel in pulmonary arterial myocytes of the rabbit using the inside-out patch clamp technique. $Mg^{2+}$ increased open probability (Po), but decreased the magnitude of single channel current. $Mg^{2+}-induced$ block of unitary current showed strong voltage dependence but increase of Po by $Mg^{2+}$ was not dependent on the membrane potential. The apparent effect of $Mg^{2+}$ might, thus, depend on the proportion between opposite effects on the Po and on the conductance of $I_{K,Ca}$ channel. In low concentration of cytoplasmic $Ca^{2+},\;Mg^{2+}$ increased $I_{K,Ca}$ by mainly enhancement of Po. However, at very high concentration of cytoplasmic $Ca^{2+},$ such as pCa 5.5, $Mg^{2+}$ decreased $I_{K,Ca}$ through the inhibition of unitary current. Moreover, $Mg^{2+}$ could activate the channel even in the absence of $Ca^{2+}.\;Mg^{2+}$ might, therefore, partly contribute to the opening of $I_{K,Ca}$ channel in resting membrane potential. This phenomenon might explain why $I_{K,Ca}$ contributes to the resting membrane potential where membrane potential and concentration of free $Ca^{2+}$ are very low.

• Asian Pacific Journal of Cancer Prevention
• /
• 제13권8호
• /
• pp.3751-3755
• /
• 2012

Aim: Tea polyphenols are known to play roles in critical steps of human lung carcinoma cell metastasis. For understanding the mechanisms whereby they inhibit tumor metastasis, the present study was conducted to investigate their effects on the adhesion of highly metastatic lung carcinoma cell lines (PG cells) to endothelial cells (EC cells) and adhesion molecule expression in vitro. Methods: The expression of CD44 or CD54 in the PG cells was detected by flow cytometry and adhesion of PG cells to EC cells was assessed by confocal microscopy double fluorescence staining. Results: The results showed that tea polyphenols: (1) inhibited the expression of CD44 and CD54, two important adhesion molecules in the PG cells in a dose-dependent manner; (2) significantly blocked the adhesion of PG cells to EC cells not only in a state of rest but also when active; and (3) influenced CD44 and CD54 expression during the adhesion process of PG cells to EC cells. Conclusions: The data indicated that the blocking role of tea polyphenols in the adhesion of PG cells to EC cells is related to CD44 and CD54. The mechanism of tea polyphenol prevention of human lung carcinoma metastasis might be through inhibiting adhesion molecule expression to block cancer cell adhesion.

• Asian Pacific Journal of Cancer Prevention
• /
• 제14권9호
• /
• pp.5017-5021
• /
• 2013

Objective: MicroRNAs (miRNAs) are a small class of non-coding, single-stranded RNAs with a critical role in genesis and maintenance of renal cancer mainly through binding to 3'-untranslated regions (3'UTR) of target mRNAs, which causes a block of translation and/or mRNA degradation. The aim of the present study was to investigate the potential effects of miR-122 in human renal cell carcinomas. Methods: The expression level of miR-122 was quantified by qRT-PCR. MTT, colony formation, invasion and migration assays were used to explore the potential functions of miR-122 in human renal cell carcinoma cells. Results: Cellular growth, invasion and migration in two A498 and 786-O cells were significantly increased after miR-122 transfection. Further experiments demonstrated that overexpression of miR-122 resulted in the increase of phospho-Akt (Ser473) and phospho-mTOR (Ser2448), then activation of mTOR targets, p70S6K and 4E-BP1. Conclusions: The up-regulation of miR-122 may play an important role in the progress of renal cancer through activating PI3K/Akt signal pathway and could be a potential molecular target for anti-cancer therapeutics.