• Title/Summary/Keyword: blast analysis

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3-Year Survival Analysis of RBM and Acid-Etched Surface Implants (RBM 표면 임플란트와 산부식 표면 임플란트의 3년 생존율에 대한 비교 연구)

  • Yoon, Dae-Woong;Kim, Moon-Seob;Jang, Han-Seung;Jin, Soo-Young;Mah, Deuk-Hyun;Jeong, Gyeong-Dal;Park, Hyun-Chun;Kim, Hee-Jung;Kim, Hak-Kyun
    • Journal of Dental Rehabilitation and Applied Science
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    • v.27 no.4
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    • pp.393-403
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    • 2011
  • The purpose of this study was to analyze and compare survival rates of resorbable blast media(RBM) surface and acid-etched surface implants being usually used in clinics. RBM surface implants (USII, Osstem, Busan, Korea) or acid-etched surface implants ($Osseotite^{(R)}$, Biomet $3i^{[TM]}$, FL, USA) were placed in edentulous area of 140 patients between January of 2005 and March of 2007. The number of implants was 304, and 152 out of them were RBM surface implants while another 152 were acid-etched surface implants. According to the evaluation items, the survey was performed before and after the implants installations. The 3-year survival rates of both kind of implants were calculated. 1. Total of 152 RBM surface implants were placed. Among them, one implant was failed, which was implanted in the posterior mandible with D2 bone quality. The failure was resulted from fracture of the fixture. Others showed good results and survival rate of RBM surface implant was 99.34%. 2. Total of 152 acid-etched surface implants were placed. Seven implants of them were failed, thus, survival rate was 95.39%. The causes of the failures were considered as infection, overheat and the lack of initial stability. In this research, both implants showed good 3-year survival rate, although RMB surface implant represented a better result.

Cloning and Characterization of a Novel Carboxylesterase Gene from Cow Rumen Metagenomic Library (소 반추위 메타게놈에서 새로운 carboxylesterase 유전자 클로닝 및 유전산물의 특성)

  • Asraful Islam, Shah Md.;Kim, Min-Keun;Renukaradhya, K. Math;Srinivasa, Reddy R.N.;Kim, Eun-Jin;Kim, Jung-Ho;Kim, Hoon;Yun, Han-Dae
    • Journal of Life Science
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    • v.20 no.9
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    • pp.1306-1313
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    • 2010
  • The gene encoding esterase enzyme was cloned from a metagenomic library of cow rumen bacteria. The esterase gene (est1R) was 2,465 bp in length, encoding a protein of 366 amino acid residues, and the molecular weight of the enzyme was 61,166 Da. Est1R of rumen cosmid library shared 5.9% amino acid identity with Est1R (P37967) of PNB carboxylesterase, 6.1% with Est1R (1EEAA) of acetylcholinesterase and 6.1% with Est1R (1H23A) of chain A. BlastP in NCBI database analysis of Est1R revealed that it was not homologous to previous known lipases and esterases. Est1R showed optimum activity at pH 7.0 and $40^{\circ}C$. On the other hand, the enzyme was found to be most active without organic solvent, followed by 95% activity with methanol, and the enzyme activity was highly affected by hexane (lost 51% activity). Therefore, the novel esterase gene est1R is likely obtainable from cow rumen metagenome and may be utilized for industrial purposes.

Impact Resistance Evaluation of RC Beams Strengthened with Carbon FRP Sheet and Steel Fiber (CFRP 시트 및 강섬유로 보강된 RC 보의 충격저항 성능 평가)

  • Cho, Seong-Hun;Min, Kyung-Hwan;Kim, Yun-Ji;Yoon, Young-Soo
    • Journal of the Korea Concrete Institute
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    • v.22 no.5
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    • pp.719-725
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    • 2010
  • The analysis and experimental program of reinforced concrete (RC) structures for resistance against such extreme loads as earthquake, blast, and impact have been carried by many researchers and designers. Under the extreme loads, a large amount of energy is suddenly exerted to the structure, hence if the structure fails to absorb the impact energy, catastrophic collapse may occur. To prevent catastrophic collapse of structures, reinforced concrete must have adeguate toughness or it needs to be strengthened. The FRP strengthening method and SFRC are studied widely in resistance of impact load because of their high energy absorption capacity. In this study, drop weight impact tests were implemented to evaluate the impact resistance of SFRC and FRP strengthened RC beam while the total steel fiber volume fractions was fixed at 0.75% carbon FRP flexural strengthened RC beams. Futhermore, to prevent the shear-plug cracks when the impact load strikes the beams, additional FRP shear strengthening method are applied. The experimental, results showed that the FRP strengthened RC SFRC beams has high resistance of shear-plug cracks and crack width and SFRC has high resistance of concrete spalling failure compared to normal RC beams. The FRP flexural and shear strengthening RC beams has weakness in the spalling failure because the impact load concentrated the concrete face which is not strengthened with FRP sheets.

Expression of Translationally Controlled Tumor Protein (TCTP) Gene of Dirofilaria immitis Guided by Transcriptomic Screening

  • Fu, Yan;Lan, Jingchao;Wu, Xuhang;Yang, Deying;Zhang, Zhihe;Nie, Huaming;Hou, Rong;Zhang, Runhui;Zheng, Wanpeng;Xie, Yue;Yan, Ning;Yang, Zhi;Wang, Chengdong;Luo, Li;Liu, Li;Gu, Xiaobin;Wang, Shuxian;Peng, Xuerong;Yang, Guangyou
    • Parasites, Hosts and Diseases
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    • v.52 no.1
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    • pp.21-26
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    • 2014
  • Dirofilaria immitis (heartworm) infections affect domestic dogs, cats, and various wild mammals with increasing incidence in temperate and tropical areas. More sensitive antibody detection methodologies are required to diagnose asymptomatic dirofilariasis with low worm burdens. Applying current transcriptomic technologies would be useful to discover potential diagnostic markers for D. immitis infection. A filarial homologue of the mammalian translationally controlled tumor protein (TCTP) was initially identified by screening the assembled transcriptome of D. immitis (DiTCTP). A BLAST analysis suggested that the DiTCTP gene shared the highest similarity with TCTP from Loa loa at protein level (97%). A histidine-tagged recombinant DiTCTP protein (rDiTCTP) of 40 kDa expressed in Escherichia coli BL21 (DE3) showed immunoreactivity with serum from a dog experimentally infected with heartworms. Localization studies illustrated the ubiquitous presence of rDiTCTP protein in the lateral hypodermal chords, dorsal hypodermal chord, muscle, intestine, and uterus in female adult worms. Further studies on D. immitis-derived TCTP are warranted to assess whether this filarial protein could be used for a diagnostic purpose.

Application effect in split doses of silicate fertilizers on rice plant (벼에 대한 규산질비료(珪酸質肥料)의 분시효과)

  • Lee, Yun Hwan;Shin, Chan Soo
    • Korean Journal of Soil Science and Fertilizer
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    • v.7 no.3
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    • pp.155-161
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    • 1974
  • In order to investigate the effect of split application of silicate fertilizers on the rice plant, this studies, designed with two kinds of silicate material, wollastonite and high concentrated fused silicate material, and 4 types of application dates on the pot experiment without chemical control against diseases, have conducted the silica analysis of rice plant and diseased rates of plant during the all growing stages. The silica contents in rice plant with basal application were increased most highly compared with the split applications during the all growing stage, but they were all much the same between the basal and split application at the harvest. High concentrated fused silicate material was more useful to increase the silica content in rice plant than wollastonite and effect of split application of silica was dominant with this fused silicate material. The diseased leaf area by rice blast was decreased from the 50% of uncontrolled plot to 20~40% by the split application with wollastonite and to 5~15% with high concentrated fused silicate material. Also percents of deseased panicles were decreased to 20% with wollastonite and to 10% with high concentrated fused silicate material.

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Correlation of XE-2100, ADVIA-120 and Manual Differential Count and Evaluation of Morphology Flag (자동혈구분석기 XE-2100, ADVIA-120와 Manual Differential Count의 상관성 및 Morphology Flag 평가)

  • Lee, Bum Hee;Byun, Nam Sub;Gee, Myung Suk;Song, Soon Young;You, Seon Woo;Park, Hyo Soon
    • Korean Journal of Clinical Laboratory Science
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    • v.36 no.2
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    • pp.144-152
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    • 2004
  • With technological advances in automatic hematology analyzers, primary and screening differential counts of white blood cells (WBC) are done with automatic hematology analyzers. They are using different measurement and analysis principles, so differences in WBC differentials and WBC morphology flag exist. This study was carried out to analyze WBC differential counts and WBC morphology flags comparing them with the manual method. Patient EDTA samples in Vacutainer requested for WBC differentials were analyzed with XE-2100. And those samples with suspect flags messages index over 100 were selected and were analyzed with ADVIA-120. Peripheral blood smear film was subsequently made. Three investigators counted 200 cells each (600 cells) in 111 Wright-Giemsa stained blood films. Between two automatic hematology analyzers, neutrophil, lymphocyte, eosinophil, and monocyte showed good correlations, but basophil had moderate correlation. Among automatic hematology analyzers and manual count, neutrophil, lymphocyte, and eosinophil had good correlations, but monocyte had moderate correlation. XE-2100 had higher monocyte, which was due to atypical lymphocyte and myeloblast. LUC in ADVIA-120 was not due to monocyte in XE-2100. Morphology flagging rates were 146.9% in XE-2100 and was 93.2% in ADVIA-120. Positive predictive values of morphology flag were 58.2% in XE-2100 and 54.4% in ADVIA-120. Flags such as atypical lymphocyte, immature granulocyte, and left shift had higher predictive values and those such as N-RBC, platelets clump, and blast had lower ones. Between automatic hematology analyzers, WBC differentials showed good correlations. Predictive values for morphology flags can be variable with changing criteria. Reviewing criteria for WBC differentials and morphology flags should be established in each laboratory with regards to size of laboratory and patients it serves.

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Performance Evaluation of Pile-Filling Material Using High Calcium Ash by Field Loading Test (고칼슘 연소재를 이용한 매입말뚝 주면고정액의 현장 재하시험을 통한 성능평가)

  • Seo, Se-Kwan;Kim, You-Seong;Lim, Yang-Hyun;Jo, Dae-Sung
    • Journal of the Korean Geotechnical Society
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    • v.34 no.6
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    • pp.17-24
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    • 2018
  • In this study, static load test and dynamic load test were performed to evaluate pile-filling material (ZA-Soil) of soil-cement injected precast pile method which was developed by using the ash of circulating fluidized boiler as a stimulant for alkali activation reaction of blast furnace slag. As a result of the static load test, the allowable bearing capacity of pile was 1,350 kN, which was the same as the result of using ordinary portland cement. And total settlement was 6.97 mm, and net settlement was 1.48 mm. These are similar to the total settlement, 7.825 mm, and net settlement, 2.005 mm of ordinary portland cement. As a result of the dynamic load test and CAPWAP analysis, the skin friction was 375.0 kN, the end bearing capacity was 3,045.9 kN, and the allowable bearing capacity was 1,368.36 kN. These results are similar to the results of using ordinary portland cement as pile-filling material.

Characteristics of Agronomy Traits to Transgenic Rice Selected by Molecular Breeding Method (분자육종기법에 의해 선발된 형질전환 벼 계통의 작물학적 특성)

  • Lee, Hyun-Suk;Kang, Hyun-Goo;Park, Young-Hie;Jung, Hee-Young;Kim, Chang-Kil;Han, Jeung-Sul;Sohn, Jae-Keun;Kim, Kyung-Min;Park, Gyu-Hwan
    • Korean Journal of Plant Resources
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    • v.21 no.5
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    • pp.388-394
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    • 2008
  • This study was carried out to develop new cultivars using the $T_5$ generation of transformed rice by PCR analysis with DNA marker in each generation $(from\;T_3\;to\;T_5)$. In the previous study, we successfully developed the transgenic rice plants over-expressing the Arabidopsis $H^+/Ca^{2+}$ antiporter CAX 1 (accession no. U57411) gene. The calcium concentration in brown rice of transgenic plants was higher than that of donor plants, Iipum, and was selected 3 lines out of 25 lines at cultured GMO field. The major agronomic traits such as culm length, panicle length and panicle number of 3 lines at transgenic plants $(T_5)$ were similar to wild type. Also these lines appeared to have disease resistance to rice blast, cold resistance as compared with donor types. The grain shape was similar to donor plant, however, the 1000 grain weight of brown rice was different from transgenic plants. These finding would be used for basic data of new variety registration.

Engineering Characteristics of Blast Furnace Slag Cement Mortar Using Chlorine Bypass System-Dust as Alkali Activator (Chlorine Bypass System-Dust를 알칼리 자극제로 사용한 고로슬래그 시멘트 모르타르의 공학적 특성)

  • Han, Min-Cheol;Lee, Dong-Joo
    • Journal of the Korean Recycled Construction Resources Institute
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    • v.8 no.2
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    • pp.235-244
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    • 2020
  • This study conducted a series of studies to offer a novel method of using CBS-dust that produced as by-product in the manufacture of cement. Four different contents of BS and CBS-dust were adopted for test parameters of this study. Mortar with 50% of W/B was fabricated. First, in the case of the fresh mortar, the flow decreased as the CBS-dust replacement rate increased, but the binder composition ratio BS 45% and 65% showed higher fl ow than Pl ain when repl acing CBS-dust 5%. In the case of air content, overall, the tendency was proportional to the CBS-dust replacement rate, and chloride tended to exceed the reference value at all replacement rates except for the CBS-dust 0% replacement. The compressive strength of the hardened mortar shows the resul t that the strength is improved when the CBS-dust is repl aced by 5% to 10%, and the CSH gel and structure formation is confirmed by microstructure analysis through the hydration reaction when the CBS-dust is replaced. Therefore, for a given condition CBS-dust is used as a early-strength admixture in a concrete secondary product that uses a large amount of admixture without reinforcing bars it can be an effective method for enhancing the strength of concrete as an alkali activator.

Evaluation of pH and Compressive Strength Development of Alpha-Calcium Sulfate Hemihydrate-based Binder (알파형 반수석고 기반 결합재의 pH 및 강도발현 평가)

  • Lee, Kyung-Ho;Yang, Keun-Hyeok
    • Journal of the Korea Institute of Building Construction
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    • v.16 no.1
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    • pp.59-65
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    • 2016
  • This study examined the compressive strength development and pH values of alpha-calcium sulfate hemihydrate(${\alpha}-CH$)-based binders developed for vegetation concrete with neutral pH between 6~7. Considering cost down and strength enhancement of the prepared binders, the ${\alpha}-CH$ was partially replaced by ground granulated blast furnace slag(GGBS), fly ash(FA), or ordinary Portland cement(OPC) by 25% and 50%. The compressive strength of mortars using 100% ${\alpha}-CH$ was 50% lower than that of 100% OPC mortars. With the increase of the replacement level of GGBS or FA, the compressive strength of ${\alpha}-CH$-based mortars tended to decrease, whereas the pH values were maintained to be 6.5~7.5. The main hydration products of ${\alpha}-CH$-based binders with GGBS or FA were a gypsum($CaSO_4$), whereas portlandite($Ca(OH)_2$) was not observed in such binders. Meanwhile, the pH values of ${\alpha}-CH$-based binders with OPC exceeded 11.5 due to the formation of $Ca(OH)_2$ phase as a hydration product. From the thermogravimetric analysis, the amount of $Ca(OH)_2$ in ${\alpha}-CH$-based binders with OPC was evaluated to be approximately 10% of the cement content.