• Journal of Animal Science and Technology
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• v.49 no.2
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• pp.183-194
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• 2007

We examined the expression patterns of the chicken TCs(tentative consensus sequences) originated from GermOnline genes in various chicken tissues, applying information from GermOnline to chicken organisms. 42 TCs among 84 chicken homologous TCs from the pool of 84 genes related to germ cell lineage in mouse(10), rat(71) and human(3) had high homology based on a BLAST search. Of these, Hmgcs2 and Sycp3 was shown to be expressed in a testis- specific manner and a reproductive organ(testis and ovary)-specific manner, respectively, by RT- PCR analysis. Crmp4, Cyct, Ldhc, Epha7, Pcsk4 and Dnmt3a are expressed in brain, testis, and ovary. The characterization of chicken genes originated from GermOnline in this research may give an enormously useful source of information related to germ cell development.

• Korean journal of applied entomology
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• v.55 no.3
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• pp.245-256
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• 2016

Elytra of Harmonia axyridis exhibit varied color patterns. In the present study, we deciphered the genetic basis for intraspecific diversity of elytra color patterns in H. axyridis, using amplified fragment length polymorphism (AFLP). Twenty-eight AFLP reactions were performed to generate a total of 2,741 bands. Of these, 20 bands were polymorphic for each color pattern. The polymorphic bands showed differences of genetic character among different color patterns of H. axyridis. Among them, ten candidate AFLP markers were color-linked. S1, S2, and S20 markers were detected in Succinea 1 and 2 variants of H. axyridis, whereas S3 and S5 were specifically detected in the Conspicua variant. S15, S18, and S19 were specific to the Succinea 2 variant. Polymerase chain reaction (PCR) products of these ten AFLP markers were sequenced. BLAST analysis of these sequences against the GenBank database revealed their homology to DNA fragments of unknown function. Based on the color-linked AFLP markers, sequence characterized amplified region (SCAR) markers were designed for PCR amplification of genomic DNA. Of the ten AFLP markers, five were successfully converted into SCAR markers, which could discriminate elytra color polymorphism in H. axyridis.

• Clean Technology
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• v.4 no.2
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• pp.41-53
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• 1998

Most of flame furnace with uncontrolled atmosphere forms oxidation scales in the surface during heat treatment. In this case, shot blasting and pickling are commonly used for reduction of oxidation scales. These processes have many disadvantages, such as long process time, high operating cost and environmental problems due to polishing dust, etc. Hence, this study aimed to develop the advanced heat treating equipment which could make a controlled atmosphere using a heatresisting structure into the flame furnace. The analysis result with SEM, EPMA, and EDX revealed that the amount of scales formed is much less than that with the conventional flame furnace. A brightness of the sample treated with the advanced process is similar compared to the treatment with vacuum furnace. In the present work, it was recommended that the inside structure of the furnace and gas line system could made up of SBB410 and STS310, respectively. The operating cost with the advanced heat treatment system could be reduced annually by forty million won. As a result of this study, it may be possible to reduce the oxidation scales. The fundamental information obtained in this study will be useful not only for improving the heat treatment process(reduction of shot blast and cleaning process), but for promoting the manufacture of bright products.

• Proceedings of the Korea Concrete Institute Conference
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• 2008.11a
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• pp.473-476
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• 2008

Due to the pursuit of high function and international price increase in the field of construction, the application of the secondary product using cement is on the increase gradually in the construction industry in the pursuit of economic cost reduction by the shortening of the construction time like Expediting and the dry construction method at the same time. However, it is in very urgent situation of measures to improve the structural performance or durable performance because it is limited for use in terms of panel in interior exterior building or functional repair reinforce as yet. Accordingly, this study is to investigate applicability of permanent Formwork like mould with the structural performance or excellent durable performance in the field of construction, and to derive optimum mixture in the performance and quality of manufacture. As a result of analysis comparison with the dynamic and durable properties of vacuum extrusion molding high toughness cement panel according to the mixture of four conditions, this study has found that the test body of mixing ECC-DP3 using small filler and large granulated blast furnace slag and powder flame retardant had excellent relative hardness and bending stress strain. The durable performance has shown excellent tendency by the decrease of porosity and enhancement of water-tightness.

• Journal of Environmental Science International
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• v.13 no.1
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• pp.99-107
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• 2004

This research is to determine the level of environmental pollution at a blasting construction area which is the origin of noise, vibration, and suspended particle, and to compare the results with other domestic and international standard data. This experiment is also to find out the effects resulting from blasting construction and to propose a plan that can decrease environmental pollution. The blasting construction area is a factory site which is about one and half million square meter and sewage disposal plant is about ninety thousand square meter. Both were selected as the areas for the tests to be conducted in determination test. The test to determine the level of noise, vibration, and total suspended particle was conducted thirty times around the blasting construction area by comparing measurement results and numerical analysis. However, as the test was not conducted in the laboratory but in the actual blasting construction area, it was not possible to do the test with the same exact conditions each true. Therefore, the test was not ideal as conditions could change from test to test. For the most part, the level of noise was below the standard level of 70dB. Every vibration test was under the standard limitation. For example, a house, 200m away was tested for noise and vibration and the level was found to be under the 0.2 cm/sec which is the standard for specialty designed cultural sites., i.e very low level. Also a buried oil pipeline that was 30m away also marked under 2.0cm/sec which is the norm for an industrial area. However, if there were an oil pipeline under the house, the amount of charging gunpowder per hole should be decreased compared to the amount used in the test. The test result for suspended particles under the standard limitation which is 24hour average 300$\mu\textrm{g}$/㎥ at a distance from blasting wavelength, but at detonator, total suspended particle from the blast origin exceeded the standard limitation. If explosion occurs when it detonates in the hole, most of the energy would be absorbed in the crushing of rocks, but some remaining energy would make noises and vibration inevitable. So the important thing is how to minimize the environmental pollution from the blasting. There should be regulations in order that the standard limitation is not exceeded, and to decrease the environmental pollution from the blasting.

• Journal of Dental Rehabilitation and Applied Science
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• v.27 no.4
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• pp.393-403
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• 2011

The purpose of this study was to analyze and compare survival rates of resorbable blast media(RBM) surface and acid-etched surface implants being usually used in clinics. RBM surface implants (USII, Osstem, Busan, Korea) or acid-etched surface implants ($Osseotite^{(R)}$, Biomet $3i^{[TM]}$, FL, USA) were placed in edentulous area of 140 patients between January of 2005 and March of 2007. The number of implants was 304, and 152 out of them were RBM surface implants while another 152 were acid-etched surface implants. According to the evaluation items, the survey was performed before and after the implants installations. The 3-year survival rates of both kind of implants were calculated. 1. Total of 152 RBM surface implants were placed. Among them, one implant was failed, which was implanted in the posterior mandible with D2 bone quality. The failure was resulted from fracture of the fixture. Others showed good results and survival rate of RBM surface implant was 99.34%. 2. Total of 152 acid-etched surface implants were placed. Seven implants of them were failed, thus, survival rate was 95.39%. The causes of the failures were considered as infection, overheat and the lack of initial stability. In this research, both implants showed good 3-year survival rate, although RMB surface implant represented a better result.

• Journal of Life Science
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• v.20 no.9
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• pp.1306-1313
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• 2010

The gene encoding esterase enzyme was cloned from a metagenomic library of cow rumen bacteria. The esterase gene (est1R) was 2,465 bp in length, encoding a protein of 366 amino acid residues, and the molecular weight of the enzyme was 61,166 Da. Est1R of rumen cosmid library shared 5.9% amino acid identity with Est1R (P37967) of PNB carboxylesterase, 6.1% with Est1R (1EEAA) of acetylcholinesterase and 6.1% with Est1R (1H23A) of chain A. BlastP in NCBI database analysis of Est1R revealed that it was not homologous to previous known lipases and esterases. Est1R showed optimum activity at pH 7.0 and $40^{\circ}C$. On the other hand, the enzyme was found to be most active without organic solvent, followed by 95% activity with methanol, and the enzyme activity was highly affected by hexane (lost 51% activity). Therefore, the novel esterase gene est1R is likely obtainable from cow rumen metagenome and may be utilized for industrial purposes.

• Journal of the Korea Concrete Institute
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• v.22 no.5
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• pp.719-725
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• 2010

The analysis and experimental program of reinforced concrete (RC) structures for resistance against such extreme loads as earthquake, blast, and impact have been carried by many researchers and designers. Under the extreme loads, a large amount of energy is suddenly exerted to the structure, hence if the structure fails to absorb the impact energy, catastrophic collapse may occur. To prevent catastrophic collapse of structures, reinforced concrete must have adeguate toughness or it needs to be strengthened. The FRP strengthening method and SFRC are studied widely in resistance of impact load because of their high energy absorption capacity. In this study, drop weight impact tests were implemented to evaluate the impact resistance of SFRC and FRP strengthened RC beam while the total steel fiber volume fractions was fixed at 0.75% carbon FRP flexural strengthened RC beams. Futhermore, to prevent the shear-plug cracks when the impact load strikes the beams, additional FRP shear strengthening method are applied. The experimental, results showed that the FRP strengthened RC SFRC beams has high resistance of shear-plug cracks and crack width and SFRC has high resistance of concrete spalling failure compared to normal RC beams. The FRP flexural and shear strengthening RC beams has weakness in the spalling failure because the impact load concentrated the concrete face which is not strengthened with FRP sheets.

• Parasites, Hosts and Diseases
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• v.52 no.1
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• pp.21-26
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• 2014

Dirofilaria immitis (heartworm) infections affect domestic dogs, cats, and various wild mammals with increasing incidence in temperate and tropical areas. More sensitive antibody detection methodologies are required to diagnose asymptomatic dirofilariasis with low worm burdens. Applying current transcriptomic technologies would be useful to discover potential diagnostic markers for D. immitis infection. A filarial homologue of the mammalian translationally controlled tumor protein (TCTP) was initially identified by screening the assembled transcriptome of D. immitis (DiTCTP). A BLAST analysis suggested that the DiTCTP gene shared the highest similarity with TCTP from Loa loa at protein level (97%). A histidine-tagged recombinant DiTCTP protein (rDiTCTP) of 40 kDa expressed in Escherichia coli BL21 (DE3) showed immunoreactivity with serum from a dog experimentally infected with heartworms. Localization studies illustrated the ubiquitous presence of rDiTCTP protein in the lateral hypodermal chords, dorsal hypodermal chord, muscle, intestine, and uterus in female adult worms. Further studies on D. immitis-derived TCTP are warranted to assess whether this filarial protein could be used for a diagnostic purpose.

• Korean Journal of Soil Science and Fertilizer
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• v.7 no.3
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• pp.155-161
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• 1974

In order to investigate the effect of split application of silicate fertilizers on the rice plant, this studies, designed with two kinds of silicate material, wollastonite and high concentrated fused silicate material, and 4 types of application dates on the pot experiment without chemical control against diseases, have conducted the silica analysis of rice plant and diseased rates of plant during the all growing stages. The silica contents in rice plant with basal application were increased most highly compared with the split applications during the all growing stage, but they were all much the same between the basal and split application at the harvest. High concentrated fused silicate material was more useful to increase the silica content in rice plant than wollastonite and effect of split application of silica was dominant with this fused silicate material. The diseased leaf area by rice blast was decreased from the 50% of uncontrolled plot to 20~40% by the split application with wollastonite and to 5~15% with high concentrated fused silicate material. Also percents of deseased panicles were decreased to 20% with wollastonite and to 10% with high concentrated fused silicate material.