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http://dx.doi.org/10.3347/kjp.2014.52.1.21

Expression of Translationally Controlled Tumor Protein (TCTP) Gene of Dirofilaria immitis Guided by Transcriptomic Screening  

Fu, Yan (Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University)
Lan, Jingchao (The Sichuan Key Laboratory for Conservation Biology on Endangered Wildlife, Developing Toward a State Key Laboratory for China, Chengdu Research Base of Giant Panda Breeding)
Wu, Xuhang (Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University)
Yang, Deying (Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University)
Zhang, Zhihe (The Sichuan Key Laboratory for Conservation Biology on Endangered Wildlife, Developing Toward a State Key Laboratory for China, Chengdu Research Base of Giant Panda Breeding)
Nie, Huaming (Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University)
Hou, Rong (The Sichuan Key Laboratory for Conservation Biology on Endangered Wildlife, Developing Toward a State Key Laboratory for China, Chengdu Research Base of Giant Panda Breeding)
Zhang, Runhui (Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University)
Zheng, Wanpeng (Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University)
Xie, Yue (Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University)
Yan, Ning (Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University)
Yang, Zhi (The Sichuan Key Laboratory for Conservation Biology on Endangered Wildlife, Developing Toward a State Key Laboratory for China, Chengdu Research Base of Giant Panda Breeding)
Wang, Chengdong (The Sichuan Key Laboratory for Conservation Biology on Endangered Wildlife, Developing Toward a State Key Laboratory for China, Chengdu Research Base of Giant Panda Breeding)
Luo, Li (The Sichuan Key Laboratory for Conservation Biology on Endangered Wildlife, Developing Toward a State Key Laboratory for China, Chengdu Research Base of Giant Panda Breeding)
Liu, Li (The Sichuan Key Laboratory for Conservation Biology on Endangered Wildlife, Developing Toward a State Key Laboratory for China, Chengdu Research Base of Giant Panda Breeding)
Gu, Xiaobin (Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University)
Wang, Shuxian (Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University)
Peng, Xuerong (Department of Chemistry, College of Life and Basic Science, Sichuan Agricultural University)
Yang, Guangyou (Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University)
Publication Information
Parasites, Hosts and Diseases / v.52, no.1, 2014 , pp. 21-26 More about this Journal
Abstract
Dirofilaria immitis (heartworm) infections affect domestic dogs, cats, and various wild mammals with increasing incidence in temperate and tropical areas. More sensitive antibody detection methodologies are required to diagnose asymptomatic dirofilariasis with low worm burdens. Applying current transcriptomic technologies would be useful to discover potential diagnostic markers for D. immitis infection. A filarial homologue of the mammalian translationally controlled tumor protein (TCTP) was initially identified by screening the assembled transcriptome of D. immitis (DiTCTP). A BLAST analysis suggested that the DiTCTP gene shared the highest similarity with TCTP from Loa loa at protein level (97%). A histidine-tagged recombinant DiTCTP protein (rDiTCTP) of 40 kDa expressed in Escherichia coli BL21 (DE3) showed immunoreactivity with serum from a dog experimentally infected with heartworms. Localization studies illustrated the ubiquitous presence of rDiTCTP protein in the lateral hypodermal chords, dorsal hypodermal chord, muscle, intestine, and uterus in female adult worms. Further studies on D. immitis-derived TCTP are warranted to assess whether this filarial protein could be used for a diagnostic purpose.
Keywords
Dirofilaria immitis; heartworm; dirofilariasis; translationally controlled tumor protein; transcriptome;
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