• Journal of the Korean Society of Tobacco Science
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• v.22 no.1
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• pp.7-12
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• 2000

This study was conducted to identify useful germ plasm sources of resistance to black shank, Phytophthora. parasitica, in Nicotiana tabacum and to characterize the interaction between host plant resistance to black shank and pathogen variability. The six internationally represen-tative set of black shank resistant and susceptible tobacco genotypes and four Korean lines were tested for black shank on the field infested with P. parasitica at Chonju Experiment Station, Korea Ginseng & Tobacco Research Institute during the 1992～1997 growing season. Almost 100% of the plants of the most susceptible genotype, Ky 14, did not survive. Complete survival of (MS Ky 14 x L8)F$_1$, which is resistant to reee 0 and ausceptible to reace 1, suggests that Race 0 is the only race of the P. parasitica in this bield. Coker 371 Gold and Beinhart 1000-1 having excellent resistance to black shank could be recommended as the parental cultivars for improving black shank resistance in Korea. Data of the diseased plants showed that KB 101 and KB 110 were medium high resistant and KB 103 and KB 108 being low resistant to black shank.

• Journal of the Korean Society of Tobacco Science
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• v.17 no.1
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• pp.57-61
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• 1995

Black shank(Phytophthora parasitira roar. nicotianae) resistant burley tobacco (Nicotiana tabacum L.) germplasms, KB 104 and KB 106, were developed by Korea Ginseng & Tobacco Research Institute. KB 104 was developed from the single cross of Burley 21$\times$Newton 77, using a modified pedigree method. KB 104 was highly resistant to black shank, and its agronomic characteristics and chemical contents were comparable to those of Burley 21, and value per 10a was slightly higher than Burley 21, KB 106 is a maternally derived doubled haploid made by N. africana method from the single cross of Burley 21$\times$ Va 509. KB 106 was also highly resistant to black shank, had two more harvestable leaves per plant and flowered three days later than Burley 21 did. Total alkaloid and nicotine contents of KB 106 were significantly lower than those of Burley 21. But its nornicotine content was higher than Burley 21 5. Key wads : Burley tobacco germplasm, Black shank resistance.

• Journal of the Korean Society of Tobacco Science
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• v.19 no.2
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• pp.83-91
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• 1997

The vein-necrosis strain or potato virus Y (PVY-Vff) and black shank (Phytophlhora parasitica roar. nicotianae) causes severe damage on burley tobacco(Wicotiana tabacum L.) in Korea, A new burley tobacco resistance to PVY and black shank, KB 110, was developed by Korea Ginseng and Tobacco Research Institute. It was developed from the cross of Burley 21 with TC 591 in 1990, and was backrossed to Burley 21 in the following season. TC 591 has resistance to PVY and moderate resistance to race 0 of black shank, but it is susceptible to tobacco mosaic vim (TMV). KB 110 was evaluated for its resistance to PVY, TMV and black shank in the greenhouse and at fields for preliminary and performance trials. KB 110 which has secreting glandular trichomes was resistant to PVY-VN, TW and black shank. It had an erect growth habit and two more leaves per plant than that of Burley 21, and matures two to three days later. It yielded approximately 3 percent more cured leaf than the standard cultivar Burley 21, but other plant characteristics were very similar to those of Burley 21. It had acceptable standards for chemical and physical characteristics of lured leaf on regional farm test in 1995-1997. KB 110 produced average yields of good quality tobaccos and was appeared to be resistant to PVY inwhere occurrence of the virus are severe chronic at burley growing area.

• Journal of the Korean Society of Tobacco Science
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• v.20 no.2
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• pp.153-159
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• 1998

The vein necrotic strain of Potato Virus Y (PVY - VN) and black shank (Phyto-phthora parasitica var. nicotianae) are the two major diseases causing severe damages especially in burley tobacco (N. tabacum L.) area in Korea. A new tobacco variety, KB 111, resistant to PVY and black shank disease, was developed by Korea Ginseng & Tobacco Research Institute in 1997. It is a male sterile(MS) F$_1$ hybrid of the cross between MS TC 613 and KB 108. KB 111 was compared to Burley 21 on the agronomic characteristics and disease resistances in performance tests: It possessed upright growth habit and flowered two days later than Burley 21. It was resistant to both PVY and black shank and yielded about 3% more cured leaf than Burley 21, but other characteristics are very simiar to those of Burley 21. The chemical composition and physical properties of the cured leaf of KB 111 were as much acceptable as those of Burley 21 while it produced average yield of good quality leaf and appeared to resistant to PVY and black shank disease on regional farm test in 1998.

• Korean Journal of Poultry Science
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• v.12 no.2
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• pp.65-73
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• 1985

More than 1,000 chickens of Korean native Yeonsan Ogolgye (Natural Monument No. 256) were used in this study in order to investigate their various external characteristics. The aims of this study were to define the Ogolgye chicken as a breed, and to provide basal data necessary for the genetic improvement of this breed. The investigated items are as follows : color of feather and toe; presense or absence of shank feather; the number of toes: color of shank and comb; the growth rates of shank, comb and feathers in various feather tracts; and finally, the livability. Data and informations were presented in tabular form in table 1 through 12. In summary, the various external traits of the Korean native Ogolgye breed can be characterized as follows. It has normal feather than silkic one as in the western type, with black color per-dominant. The frquency of white feather color is very low. The color of toes is devided into two categories; black toes in black plumage lines, and white toes in white plumage lines. Both seres have single combs with black color. The shank is gray- black and featherless. The breed has normally four toes. The eye color is black.

• The Plant Pathology Journal
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• v.16 no.4
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• pp.222-226
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• 2000

In disease surveys from 1986 ti 1998, disease incidence of tobacco black shank was gradually increased in burley tobacco from 1996. To study the causes of the disease occurrence, one hundred and fourteen isolates of Phytophthora parasitica var. nicotianae (Ppn) were collected from burley tobacco-growing areas in the southern part of Korea during 1996-1997, and tested in vitro for meatlaxyl sensitivity which was determined by measuring the mycelial growth on corn meal agar (CMA) amended with metalaxyl. Of the tested isolates, 78.1% showed sensitive to metalaxyl, having $\textrm{ED}_{50}$ values less than 1.0 $\mu\textrm{g}/$\textrm{ml}, while 1.7% was resistant weth $\textrm{ED}_{50}$ greater than 100 $\mu\textrm{g}/$\textrm{ml}. Ppn isolates from three provinces, Chungnam, Chonbuk and Chonnam showed similar distributions of metalaxyl sensitivity. Metalaxyl-resistant isolates were not significantly different from metalaxyl-sensitive ones in mycelial growth rate, chlamydospore formation capacity and size of the spore, and pathogenicity on tobacco plant (cv. Burley 21). These results suggest that the metalaxyl-resistant Ppn in burley tobacco may be one of the major factors to cause the higher occurrence of the tobacco black shank in the burley tobacco-growing area.

• Research in Plant Disease
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• v.9 no.4
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• pp.224-228
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• 2003

Five commercial chemicals, Dimethomorph, Foseyl-Al, Oxadixyl + Mancozeb, Propamocarb hydrochloride, and Metalaxyl, were tested for control of tobacco black shank (Phytophthora nicotianae) and/or delaying buildup of the resistant population to Metalaxyl which has been used for control of the disease in Korean tobacco farms. Propamocarb hydrochloride seemed to have a cross-resistance to the Metalaxyl resistant isolates showing similar response with Metalaxyl in vitro. Meanwhile, Dimethomorph+Mancozeb and Oxadixyl+Mancozeb were selected to be the promising chemicals which are able to be alternative to Metalaxyl for the black shank control in accordance with in vitro and in field trial.

• Journal of the Korean Society of Tobacco Science
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• v.14 no.2
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• pp.97-103
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• 1992

KB 101 is a bacterial wilt(Pseudomonas solanaceamm E.F. Smith) and black shank (Phytophthora nicotianae Breda de Haan Var. nicotianae Waterhouse) resistant cultivar of burley tobacco (Nicotiana tabacum L.) KB 101 was developed by the Korea Ginseng&Tobacco Research Institute, and released in 1987. KB 101 was developed from a single plant selection in the F2 generation derived from the double cross, [(Burley 21X Burley 37) X (Burley 64X Ky 16)]. Burley 37 and Burley 64 were the source of resistance to bacterial wilt and black shank. Yield trials were conducted in the Fs through F6 generations at the four Exp. Stn. of Korea Ginseng &Tobacco Research Institute as JB 7705-1. On-farm yield trials were conducted in the F7 through F9 generations at the 45 locations of burley tobacco growing area from 1984 to 1986 as KB 101. KB 101 has an erect growth habit similar to that of Burley 21: plant size is larger and has more leaves than those of Burley 21. It is late maturing cultivar that flowers approximately 3 days later than Burley 21. The physical characteristics and chemical composition of KB 101 were similar to those of Burley 21.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.4 no.1
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• pp.103-108
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• 1968

Resistance to various diseases of Korean native varieties were investigated in field. The results obtained are summarized as follows: 1) The varieties Ubangtchio and Osib-eubthio were moderately resistant to black shank, but Suantchio, Usultchio and Gwangtchio were slightly resistant. Dixie Bright 101, $H_2$ and Bright Yellow 4 were more resistant to black shank than any of the Korean native varieties. 2) Mokgitchio, Ubangtchio and Osib-eubtchio were moderately resistant to black root rot, but Useultchio Suantchio and Hoetchio were susceptible than Others. 3) Muktchio, Hoetchio and Ubangtchio were slightly resistant to wild fire. 4) Osib-ebthio was highly resistant to brown spot, but Ubangtchio, Hyangtchio and Mokgitchio were moderately resistant and Useultchio was susceptible to brown spot, but it was resistant than Bright ellow 4 or Bernhart 1000-1. 5) Hyangtchio was slightly resistant to CMV-Y. According to these results, except Virus disease, Ubangtchio was resistant to all of the above mentioned diseases. Osib-eubtchio, Hoetchio, Hyangtchio and Suantchio showed proferable disease resistant and these might be well utilized as breeding materials.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.8 no.1
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• pp.105-110
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• 1970

Resistance to Black shank of various varieties were investigated in field and incubator. The results were summarized as follows; 1) In occurrence of this disease, yellow tobacco varieties were ranged from 4 days to 12 days after inoculated. 2) Korean native varieties were ranged from 6 days to 20 days. 3) Orient tobacco takes from 8 days to 17 days in occurrence of this one. 4) Japanese varieties ranged from 4 days to 3 days in occurrence of this disease. 5) Dark air-curing tobacco was ranged from 5 days to 14 days. 6) Chinese variety was ranged from 6 days to 11 days. Each varieties of resistant to Black shank was as follows; 1) Yellow tobacco varieties; Cash, Oxford-1, Oxford-3, N.C-75, N.C-95, Golden-cure Delcrest. 2) Native varieties; Sinzongtchio, Muktchio Sosa-3 Mokgitchio. 3) Japanese varieties; Hatano Outaruma Maruha. 4) Chinese variety; Gkzuoe, Yung-zung, Tongzuoe. 5) Orient tobacco; Samsun, Baffra, Xanthix. 6) Dark air-curing varieties; B-Hw, Connecticut-Broad leaf, Connecticut-Havana, Tennessee-Black.