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Comparison of the Food Quality of Hybrid Grouper Epinephelus fuyscoguttatus×E. labnceolatus and Hybrid Longtooth Grouper E. moara×E. lanceolatus (대왕범바리(Epinephelus fuscoguttatus×E. lanceolatus)와 대왕자바리(E. moara×E. lanceolatus)의 식품학적 품질비교)

  • Lee, Woo Jin;An, Byoung Kyu;In, Jung Jin;Han, Hyeong Gu;Park, Jong-Yeon;Bang, In-Chul;Shim, Kil Bo
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.55 no.2
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    • pp.129-136
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    • 2022
  • The nutrient composition of the muscle of the hybrid grouper (Epinephelus fuscoguttatus×E. lanceolatus, HG) and the hybrid longtooth grouper (E. moara×E. lanceolatus, HLG) was measured as a function of body weight in this study. The crude protein and lipid levels in HG were 21.0-21.2 g/100 g and 1.9-3.4 g/100 g, respectively. HLG contained 20.6-20.7 g/100 g protein, and 3.9-5.5 g/100 g crude lipid. The total amino acid contents of HG were 18,829.7-18,980.2 mg/100 g and that of HLG was 17,793.7-19,293.7 mg/100 g. The mean saturated fatty acid content was 0.61-1.09 g/100 g for HG and 1.27-1.77 g/100 g for HLG. Monounsaturated and polyunsaturated fatty acid levels were 0.59-1.02 g/100 g (Monoene), 0.67-1.11 g/100 g (Polyene) in HG, which were lower than the 1.21-1.78 (Monoene), 1.22-1.69 g/100 g (Polyene) found in HLG. The highest mineral content (K and P) of HG was 510.13-517.05 mg/100g and 231.59-247.67 mg/100 g, while that of HLG was 518.81-523.59 mg/100 g and 257.51-248.84 mg/100 g, respectively. In conclusion, there is potential for expanding the commercial utilization of HG and HLG as food resources as they are both nutrient-rich.

Antioxidative and Circadian Rhythm Regulation Effect of Quercus gilva Extract

  • HUH, Jin-Sung;LEE, Sora;KIM, Dong-Soo;CHOI, Myung Suk;CHOI, Hyunmo;LEE, Kyung-Ha
    • Journal of the Korean Wood Science and Technology
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    • v.50 no.5
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    • pp.338-352
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    • 2022
  • Herein, water and ethanol extracts were obtained from the leaves, branches, kernels, and pericarp of Quercus gilva and subsequently analyzed for antioxidant activity and circadian rhythm regulation effects. Candidate components that may affect circadian rhythm and antioxidant activity were investigated to discover potential functional materials. Antioxidant activity was analyzed via 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging activity assays, showing that the hot water extract exhibited higher activity than that of the ethanol extract. In particular, the branch extract showed high antioxidant activity. By measuring total contents of polyphenols, flavonoids, and tannins, the hot water branch extract showed the highest concentrations, highlighting their significant contribution to the antioxidant activity. Examination of the circadian rhythm regulation of each extract showed that the ethanol extract exhibited greater impacts on the circadian rhythm amplitude compared to the water extract. The branch ethanol extract induced circadian rhythm amplitude changes via clock gene Bmal1 expression regulation. Determination of 12 phenolic compound concentrations showed that the branch ethanol extract contained many phenolic compounds, including catechin. This suggests that these com- pounds affected circadian rhythm regulation. In conclusion, the hot water branch extract has potential as an natural antioxidant material, while the corresponding ethanol extract has potential as a functional material for regulating circadian rhythm.

How do Elementary Students Classify the Branches of Science?

  • Kwon, Sung-Gi;Nam, Il-Kyun
    • Journal of The Korean Association For Science Education
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    • v.29 no.3
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    • pp.329-347
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    • 2009
  • Science curriculums for elementary schools were, traditionally, developed to be balanced in content and contain equal proportions of the four branches of science: physics, chemistry, biology, and earth science. To develop a successful science curriculum, we asked some questions about how elementary students recognize these branches and about what they think of the domains of science in the science curriculum. Our study was designed to investigate how elementary students classify the domains of science in the curriculum. Previous research (Lee et al., 2001) seemed not to be successful, because verbal expressions in that research might be inappropriate for elementary students who were unaccustomed to the technical language of science. For this reason, instead of using only words, we developed image card instruments, made of picture duplicates of the introductory covers of each unit in the 3$^{rd}$, 4$^{th}$, and 5$^{th}$ grades' science textbooks. We asked students to classify these cards into their own categories and record the reasons for classifying them. The ratio and distribution of the units was then analyzed to identify their view of the science domains. 30% of the 4$^{th}$ grade students created the following categories: 'nature,' 'observation,' 'seasons,' 'living things,' 'sounds,' 'separating,' and 'the things necessary for everyday life'. In the case of the 5$^{th}$ grade, over 30% created the categories of 'living things,' 'weight,' and 'water.' Over 30% of the 6$^{th}$ grade created the categories of 'nature,' 'light,' 'water,' 'living things,' 'solution,' 'fire,' 'properties of an object,' and 'experiment.' Upon scrutinizing the above results, we discovered that the science domains selected by students into three types of domains: academic contents and concepts; activities related to a science class; and lessons and experiences in students ' lives. The last category was a new, complex kind of domain. We concluded that students did not utilize the four branches of science when constructing their own domains of science. Instead, they created many alternative domains, which reflected students' thoughts of and their experiences. The educational needs of elementary students suggest that when organizing science curriculum as 25 % allocation of the four science branches, newly-created domains should be considered.

A Study on Update of Bioinformatics Contents (생명정보 콘텐트 업데이트에 관한 연구)

  • Ahn, Bu-Young;Han, Jeong-Min;Hong, Soon-Chan;Lee, Sang-Ho
    • Proceedings of the Korea Information Processing Society Conference
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    • 2007.11a
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    • pp.452-455
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    • 2007
  • 생명과학 기술의 급속한 발달로 인류 복지 증진에 많은 기여를 하였지만 아직도 각종 질병 등으로 많은 사람들이 고통 받고 있으며, 이를 극복하기 위한 연구 및 기술개발은 세계 각처에서 계속되고 있다. 이러한 연구 및 기술개발의 결과로 산출되는 생명정보 데이터의 양은 기하급수적으로 증가하고 있기에 이런 방대한 양의 생명정보 데이터를 분석하고 분석된 데이터에서 인류 복지에 유용한 정보를 얻기 위한 생명정보학(Bioinformatics)이 등장하게 되었다. 이에, 한국과학기술정보연구원(KISTI)은 IT 기반 생명정보 인프라 구축의 중심기관으로 CCBB(Center for Conputationa Biology & Bioinformatics) 웹사이트를 운영하고 있다. CCBB는 전산학적인 기술을 이용한 생명현상 연구를 지원하기 위하여 21종의 생명정보 콘텐트(DB 및 분석도구)를 수집 분석 구축 제공하고 있다. 이 중에서 GenBank, PDB, PIR, Swiss-prot 등의 데이터베이스는 KISTI에서 개발한 KRISTAL 검색엔진을 통하여 국내에서도 빠르고 쉽게 검색 가능하도록 자체 구축하고 있으며, 이와 더불어 BLAST, FASTA, ClustalW 등의 주요 분석 도구 또한 제공하고 있다. 본 논문에서는 CCBB에서 제공중인 21종의 콘텐트 중에서 GenBank, REBASE, GeneCards, InterProScan 등 4종의 대용량 고효율 생명정보 콘텐트의 소개 및 업데이트 방법에 관한 내용을 기술하고자 한다.

Quantitative Analysis of Sesamin and Sesamolin in Various Cultivars of Sesame (참깨 품종별 Sesamin, Sesamolin 함량 변이)

  • Kim Geum-Soog;Kim Dong-Hwi;Jeong Mi-Ran;Jang In-Bok;Shim Kang-Bo;Kang Chul-Hwan;Lee Seung-Eun;Seong Nak-Sul;Song Kyung-Sik
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.49 no.6
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    • pp.496-502
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    • 2004
  • The development of sesame varieties which contain high contents of lignan compounds has been progressed in Korea. This study was carried out to get the basic information for the breeding of high quality sesame varieties and the development of health functional food sources using lignan compounds from sesame. The contents of sesamin and sesamolin were $4.08\pm1.74$ and $2.47\pm0.68mg/g$, respectively, from sesame cultivated in 2002 and those were $4.08\pm1.51$ and $2.48\pm0.69mg/g$, respectively, from sesame cultivated in 2003. The content of sesamine was high in the order of Danbaek (6.22 mg/g), Seongbun (5.94 mg/g), Namda (5.83 mg/g), and Naman (5.59 mg/g) producted in 2002 and Seongbun (6.27 mg/g), Dasak (5.53 mg/g), Danbaek (5.50 mg/g), chinbaek (5.45 mg/g), and Seodun (5.41 mg/g) producted in 2003. The content of sesmoline was high in the order of Hwangbaek (3.27 mg/g), Seongbun (3.26 mg/g), Annam (3.22 mg/g), Hanseom (3.17 mg/g), and Danbaek (3.16 mg/g) producted in 2002, and Seongbun (3.21 mg/g), Seodun (3.14 mg/g), Pungan (3.10 mg/g), Naman (3.09 mg/g), and Danbaek (3.04 mg/g) producted in 2003. The contents of sesamin and sesamolin of Yangbaek, which has used as a standard variety for sesame breeding, were relatively lower than in any other varieties. These resluts suggest that Yangbaek is not the proper variety as a standard variety for development of high quality sesame. In conclusion, proper good variety as a standard variety for sesame breeding must be determined after careful consideration on the major quality factors including crude oil and lignan compounds as well as agricultural characteristics to develop high quality sesame varieties containing high contents of lignan compounds.

Changes of chemical Composition According to the Ensiling Periods of Total Mixed Fermentation Feeds using Rice Straw and Green Forages (청초와 볏짚을 이용한 완전배합발효사료의 저장기간에 따른 화학조성분의 변화)

  • Lee, H.J.;KIm, W.H.;Kim, H.S.;Lim, K.B.;Ahn, B.S.;Cho, K.K.;Kang, S.H.;Kang, S.K.;Lee, H.G.;Woo, J.H.;Choi, Y.J.
    • Journal of Animal Science and Technology
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    • v.44 no.6
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    • pp.769-782
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    • 2002
  • Three kinds of green forages(rye, oats and mixed forages) was harvested and mixed with rice straw, wheat bran and 2 grains(corn and soybean), which harvested 2 different dates(common harvesting dates, 7 days early to common harvesting dates). And each mixture was ensiled in 6 poly vinyl chlorides that was 60 liter, immediately. They were opened at 0, 5, 10, 25, 35, 60 and 100 days after ensiling for chemical analysis. And its effects of those TMFFs on feed values were observed. Average contents of water, crude protein, ADF, NDF, Ca and P of formulated TMFs were 72 to 75%, 14.75 to 18.24, 12.47 to 19.07, 39.82 to 47.01, 0.99 to 1.07 and 0.38 to respectively. Crude protein content was the highest in the mixed forages-TMFF and the lowest in the rye-TMFF. The ADF and NDF contents of rye-TMFF were higher than orthers. And CP, ADF, NDF, TDN, P and Ca contents were no significant difference among treatments regardless of storage period and harvest time, but all treatments indicated good quality. Intenal temperatures of TMFF were shown to be 1 to 5$^{\circ}C$ higher than ambient temperatures. The temperature of the Oat-TMFF formulated during winter sustained higher to the level of 6${\sim}$9$^{\circ}C$ for 10 days. The pH of TMFF were 4.0 to 4.2 and the content of $NH_3$-N was shown to be 7.79 to 8.23mg/$d{\ell}$. In the VFA contents, any tendency was not shown at all treatments depending on harvest time. Even though rye-TMFF showed the lowest VFA value. At all treatments except rye-TMFF, propionate production was increased and stable after 25 days of storage. Digestibility of rice straw from TMFF on DM basis was 15${\sim}$20% higher compared with non-treated rice straw.

Effects of Inorganic Nutrients and Heavy Metals on Growth and Pigmentation of the Green Alga, Ulva pertusa Kjellman (녹조 구멍갈파래(Ulva pertusa Kjellman)의 생장 및 색소조성에 미치는 무기 영양염류 및 중금속의 영향)

  • 김장균;한태준
    • Korean Journal of Environmental Biology
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    • v.17 no.4
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    • pp.427-438
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    • 1999
  • Differential growth of Ulva pertusa Kjellman was observed in response to different photon irradiances and seawater. Growth rate of U. pertusa cultivated in the seawater collected from the East Sea was significantly higher than that in the seawater collected from the Yellow Sea. Optimal growth was found at $100{\mu} molm^{-2} s^{-1}$ in both cases. Chlorophyll contents of U. pertusa grown in the east sea water were higher than that cultivated in the west sea water at irradiances lower than $60{\mu} molm^{-2} s^{-1}$. At irradiances higher than $100{\mu} molm^{-2} s^{-1}$, there was no difference in chlorophyll content in between the two different sea waters with tendency that pigmentation decreased with increasing photon irradiances. Nitrate concentration in the west sea water was 2-fold higher than that in the east sea water, while phosphorus concentrations (0.03 ppm) were similar. Concentrations of $Cu^{2+}$ and $Pb^{2+}$ were 0.004 and 0.003 ppm respectively which are far below environmental standard concentrations (0.02 ppm for $Cu^{2+}$ and 0.1 ppm for $Pb^{2+}$). Taking those data into account, we have done laboratory investigations into the effects of inorganic nutrients and heavy metals on U. pertusa. As nitrate concentration increased from 0.5 to 5 ppm, growth rate of U. pertusa increased, but different concentrations of phosphorus did not cause any differential effect. On the other hand, chlorophyll contents increased with increasing phosphorus concentrations. Copper of U. pertusa be toxic decreased the growth and pigmentation as the concentration increased, whereas lead showed no such effect. Concentrations of $Cu^{2+}$ employed in the present study were much higher than those in ambient seawater. Intermittent soaring of $Cu^{2+}$ level as observed in natural seawater could, however, seriously damage the growth behaviour of U. pertusa.

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Reproductive Progress and Heavy Metal Contamination of Feral Pigeons (Columba livia) in Seoul and the Ansan Industrial Complex areas (도심 및 공단지역에 서식하는 비둘기의 중금속 축적과 번식 경과 비교)

  • 남동하;이두표;구태회
    • Korean Journal of Environmental Biology
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    • v.21 no.2
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    • pp.149-157
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    • 2003
  • This study focused on relationships between Pb and Cd concentrations and the difference of success reproductive progress in urban (Seoul) and industrial complex (Ansan) areas. Results of the Pb analysis for the feral pigeons from Seoul (egg contents:1.64 $\mu\textrm{g}$ wet $g^{-1}$, adults in bones: 29.5 $\mu\textrm{g}$ wet $g^{-1}$ and the Ansan industrial complex (egg contents: 1.13 $\mu\textrm{g}$ wet $g^{-1}$, adults in bones: 10.5 $\mu\textrm{g}$ wet g-1) showed that the Pb level of eggs and adults is significantly different between the two colonies (p<0.05). Cd concentrations in liver and kidneys of adult pigeons were also significantly different between Seoul(liver: 0.24 ${\mu}g$ wet $g^{-1}$, kidney: 1.05 $\mu\textrm{g}$ wet $g^{-1}$ and the Ansan (liver: 0.14 $\mu\textrm{g}$ wet $g^{-1}$, kidney: 0.43 $\mu\textrm{g}$ wet $g^{-1}$ colonies (P<0.05). Clutch size of Feral Pigeons living in Seoul was similar between the two colonies, 1.9$\pm$0.3 in Seoul and 2.0$\pm$0.0 in Ansan. The length, breadth, and thickness of eggs were not significantly different between the two colonies (p>0.05). Incubation period in Seoul (17.8 days) did not differ from the Ansan (17.4 days). No difference in growth rate (body weight, wing length, and tarsus length) was found between the two test groups (p>0.05). In Seoul, 65.2% were hatching, and 42.1% fledging. The Proportion of hatching and fledging in the Ansan was 60.7% and 45.0%, respectively. The significant differences between the two colonies for reproductive sucess were not found (p>0.05). With regard to the reproductive effects to the heavy metals, the Pb and Cd concentrations feund in the two colonies were not as high as those considered in results of toxic effects in other species.

Nutrient Removal Potential of water Hyacinth Cultured in Nutrient-enriched Water and Swinery Wastewater (부레옥잠의 수중영양염 제거 잠재력에 관한 고찰)

  • 전만식;김범철
    • Korean Journal of Environmental Biology
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    • v.17 no.1
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    • pp.117-124
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    • 1999
  • Nutrients removal by water hyacinth, Eichhornia crassipes (Mart.) Solms from nutrient enriched waters and swinery wastewater were evaluated. The contents of nitrogen and phosphorus of dried water hyacinth increased from 1.4 to 3.3% and 0.21 to 0.80% when water hyacinth available N and P in the culture medium were increased from 0.7 to 5.0 mgN/1 and 0.06 o 1.5 mgP/l. respectively. Maximum N and P contents were found to be 4.1 and 0.90%, respectively. The significant relationship was observed between the standing crop of water hyacinth and the biomass yield per unit area and time. Biomass yield increased gradually until standing crop reached 15 kg wet wt./m$^2$, and then rapidly decreased. The high biomass yield of up to 0.5 kg/m$^2$/day was obtained in the range of 7 to 20 kg/m$^2$of standing crop. The potential removal rates of N and P by the water hyacinth during summer were found to be 2,250 to 2,710 mgN/m$^2$/day and 570 to 595 mgP/m$^2$/day, respectively, when 15 kg/m$^2$in standing crop and nutrient concentrations of culture medium were ranged from 1.24 to 6.2 mgP/1 and 3.2 to 32.5 mgN/1, respectively, Inorganic N and P concentrations of swinery wastewater were in the range of 82 to 121 mgN/1 and 22 to 79 mgP/1, respectively. Nitrogen and P removal rates of water hyacinth cultured in swinery wastewater were found to be in the ranges of 2,000 to 2,600 mgN/m$^2$/day and 157 to 254 mgP/m$^2$/day, respectively, at 10 times diluted water of swinery wastewater.

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Energy Budgets for the Developmental Stages of Palaemon macrodactylus (Palaemon macrodactylus의 생활사에 따른 에너지 수지)

  • CHIN Pyung;KIM Heung-Yun;SIN Yun-Kyong
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.25 no.5
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    • pp.341-358
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    • 1992
  • In order to estimate energy budgets of Palaemon macrodactylus, larvae of the shrimp were reared in the laboratory at constant conditions $(25^{\circ}C: 31-32\%o),$ and then juvenile to adult of the shrimp were reared at $15^{\circ}C\;and\;25^{\circ}C$ in the laboratory. Energy used by the reared shrimps were calculated from estimates of data on feeding, growth, molting, metabolism, nitrogen excretion, and energy content. Juveniles and adults reared in the laboratory, which fed on Artemia nauplii, had an average daily growth rates of 0.079 mm/day at $15^{\circ}C\;and\;of\;0.122mm/day\;at\;25^{\circ}C$. The average growth factor* of P. macrodactylus males and females ranged from $3.2\%$ for adult to $13.2\%$ for juveniles individuals, respectively. Intermolt periods were related to body size of the shrimp and to temperature. Average laboratory growth curves were calculated from data on growth factors and intermolt periods to body size of the shrimp at $15^{\circ}C\;and\;25^{\circ}C$. The calorie contents of the shrimp, their molts, eggs and larvae were determined by biochemical composition and oxygen bomb calorimetry. The average amount of energy used in growth for larvae and juvenile to adult were 4.94 cal and 4.55 cal per dry weight in milligram, respectively. The ammount of oxygen used in metabolism was calculated from size, temperature-specific respiration rate. To convert the ammount of oxygen used in respiration into the equivalent energy lost heat was estimated from the data on chemical composition for the larvae and adult, the values was 4.58 cal/ml $O_2$. The energy content per egg was 0.078 cal. The assimilation efficiency estimated by nitrogen content of food and egested faeces gave $61.5\%$ for the larvae. The efficiencies for juvenile to adult ranged between $79.4\%$ and $90.1\%$ The gross growth efficiencies $(K_1)$ and net growth efficiencies $(K_2)$ of P macrodactylus showed $18.33\%\;and 32.63\%$ for total larval stages, ranged from $21.30\%\;to\;31.04\%\;and\;from\;30.03\%\;to\;39.34\%$ for juvenile to adult, respectively.

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