• 제목/요약/키워드: biological study

검색결과 14,774건 처리시간 0.041초

IL-18 gene expression pattern in exogenously treated AML cells

  • Seo, Min-Ji;Park, Min-Ha;Yook, Yeon-Joo;Kwon, Young-Sook;Suh, Young-Ju;Kim, Min-Jung;Cho, Dae-Ho;Park, Jong-Hoon
    • BMB Reports
    • /
    • 제41권6호
    • /
    • pp.461-465
    • /
    • 2008
  • IL-18 production may enhance immune system defense against KG-1 cells ; NB4 cells, which are associated with good prognosis, do not produce IL-18. In this study, we treated KG-1 cells with IL-18 and used microarray technology to assess subsequent effects on gene expression. In UniGene-array of 7488 human genes, expression of 57 genes, including stress related genes, increased at least 2-fold, whereas expression of 48 genes decreased at least 2-fold. Following exogenous exposure of KG-1 cells to IL-18, expression of CRYGC, $NF{\kappa}BIA$ and NACA gene were monitored. The latter is a transcriptional coactivator potentiating c-Jun-mediated transcription.$NF{\kappa}BIA$ is an inhibitor of $NF{\kappa}B$, and affects growth regulation, apoptosis and hypoxic stress. Studies, such as this one, are beginning to clarify the differences between cells associated with good and bad cancer prognoses, which may ultimately assist in medical treatment for acute myeloid leukemia.

Stress-induced secondary carotenogenesis in Coelastrella rubescens (Scenedesmaceae, Chlorophyta), a producer of value-added keto-carotenoids

  • Minyuk, Galina;Chelebieva, Elina;Chubchikova, Irina;Dantsyuk, Natalia;Drobetskaya, Irina;Sakhon, Evgenii;Chekanov, Konstantin;Solovchenko, Alexei
    • ALGAE
    • /
    • 제32권3호
    • /
    • pp.245-259
    • /
    • 2017
  • We report on the culture growth and stress-induced secondary carotenogenesis in a biotechnologically promising but largely unexplored chlorophyte Coelastrella rubescens strain Vinatzer/Innsbruck V 195. Changes in the cell morphometry, biomass accumulation, its carotenoid and fatty acid profiles were followed in the cultures supplemented with either inorganic ($CO_2$) or organic (sodium acetate) carbon on the background of low-pH stress. Collectively, the results of the study characterize C. rubescens as a biotechnologically promising, potentially double-purpose organism. It produces several secondary keto-carotenoids with a considerable proportion of astaxanthin and canthaxanthin. At the same time, the cell lipid fatty acid profile of this microalga is suitable for obtaining a high-quality biodiesel complying with the strictest EN14214 European standard.

Six unrecorded macrofungi from the Royal Tombs(Donggureung and Seooreung) of the Joseon Dynasty and Jongmyo Shrine, Korea

  • Cho, Hae Jin;Lee, Hyun;Li, Vladimir;Jargalmaa, Suldbold;Kim, Nam Kyu;Kim, Min-Ji;Lim, Young Woon
    • Journal of Species Research
    • /
    • 제7권1호
    • /
    • pp.1-8
    • /
    • 2018
  • Macrofungi are visible to the naked eye and play important ecological roles in nutrient cycles as decomposers and symbionts in forest ecosystems. Collectively, macrofungi have great potential as valuable resources for food, cosmetics, and medicinal uses. We surveyed the Royal Tombs (Donggureung and Seooreung) of the Joseon Dynasty and Jongmyo Shrine, where the surrounding vegetation is well-preserved, to investigate indigenous macrofungi. During surveys in 2015-2016, we discovered six macrofungi that were previously not recorded to Korea. They were identified to the species level using morphological features and phylogenetic analysis based on the internal transcribed spacer region: Cruentomycena kedrovaya, Dacrymyces aureosporus, Laetiporus versisporus, Parasola setulosa, Piptoporellus soloniensis, and Pluteus longistriatus. The detailed morphological descriptions and molecular analysis are provided in this study.

First report of six Sordariomycetes fungi isolated from plant litter in freshwater ecosystems of Korea

  • Goh, Jaeduk;Mun, Hye Yeon;Jeon, Yu-Jeong;Chung, Namil;Park, Young-Hwan;Park, Sangkyu;Hwang, Hyejin;Cheon, Wonsu
    • 한국균학회지
    • /
    • 제48권2호
    • /
    • pp.103-116
    • /
    • 2020
  • Freshwater ecosystems provide a complex environment for microorganisms. In this study, we isolated diverse fungal strains from plant litter in freshwaters. These strains were identified using molecular phylogenetic analyses of rDNA and/or other gene sequences (TUB, GAPDH, and EF1). In addition, we examined their morphological characteristics by microscopy and cultural characteristics on several media. We identified six previously unrecorded Sordariomycetes species in Korea, i.e., Colletotrichum godetiae, Discosia rubi, Robillarda sessilis, Monochaetia dimorphospora, Idriella lunata, and Phialemoniopsis endophytica. Of these, D. rubi and M. dimorphospora exhibited high extracellular amylase, lipase, and protease activities, suggesting that these fungal isolates might play an important role as decomposers in freshwater ecosystems. Plant litter could thus be a good source for isolating and investigating previously undocumented fungal species in freshwater environments.

Expression and phosphorylation analysis of soluble proteins and membrane-localised receptor-like kinases from Arabidopsis thaliana in Escherichia coli

  • Oh, Eun-Seok;Eva, Foyjunnaher;Kim, Sang-Yun;Oh, Man-Ho
    • Journal of Plant Biotechnology
    • /
    • 제45권4호
    • /
    • pp.315-321
    • /
    • 2018
  • Molecular and functional characterization of proteins and their levels is of great interest in understanding the mechanism of diverse cellular processes. In this study, we report on the convenient Escherichia coli-based protein expression system that allows recombinant of soluble proteins expression and cytosolic domain of membrane-localised kinases, followed by the detection of autophosphorylation activity in protein kinases. This approach is applied to regulatory proteins of Arabidopsis thaliana, including 14-3-3, calmodulin, calcium-dependent protein kinase, TERMINAL FLOWER 1(TFL1), FLOWERING LOCUS T (FT), receptor-like cytoplasmic kinase and cytoplasmic domain of leucine-rich repeat-receptor like kinase proteins. Our Western blot analysis which uses phospho-specific antibodies showed that five putative LRR-RLKs and two putative RLCKs have autophosphorylation activity in vitro on threonine and/or tyrosine residue(s), suggesting their potential role in signal transduction pathways. Our findings were also discussed in the broader context of recombinant expression and biochemical analysis of soluble and membrane-localised receptor kinases in microbial systems.

Seven Yeast Strains Isolated from Freshwaters for the First Record in Korea

  • Jeon, Yu Jeong;Park, Sangkyu;Hwang, Hyejin;Park, Yeong Hwan;Cheon, Wonsu;Goh, Jaeduk;Chung, Namil;Mun, Hye Yeon
    • 한국균학회지
    • /
    • 제48권4호
    • /
    • pp.523-531
    • /
    • 2020
  • This study focused on the isolation and characterization of wild yeasts in Korea. The yeasts were identified by phylogenetically analyzing the D1/D2 domains of the 26S rDNA regions. Consequently, we identified seven strains, NNIBRFG856, NNIBRFG3732, NNIBRFG3734, NNIBRFG3738, NNIBRFG3739, NNIBRFG5497, and NNIBRFG6049, which were confirmed to be Kabatiella microsticta, Pichia membranifaciens, Candida vartiovaarae, Candida sake, Debaryomyces hansenii, Candida railenensis, and Schwanniomyces polymorphus, respectively, all of them being new in Korea. Morphological and cultural characteristics of these yeast species were investigated. None of the strains formed ascospores or pseudomycelia. Moreover, these yeasts grew in a pH range of 4-8. NNIBRFG3732, NNIBRFG3738, NNIBRFG3739, NNIBRFG5497, and NNIBRFG6049 were halotolerant or halophilic, and NNIBRFG3732, NNIBRFG3734, and NNIBRFG6049 grew in vitamin-free medium. NNIBRFG3732, NIBRFG3739, and NNIBRFG6049 grew at 35 ℃, but not at 40 ℃.

Isolation and Characterization of Eleven Unrecorded Pezizomycotina Species from Freshwater Ecosystems in Korea

  • Goh, Jaeduk;Jeon, Yu-Jeong;Mun, Hye Yeon;Chung, Namil;Park, Young-Hwan;Park, Sangkyu;Hwang, Hyejin;Cheon, Wonsu
    • 한국균학회지
    • /
    • 제48권4호
    • /
    • pp.423-443
    • /
    • 2020
  • Freshwater fungi are a poly-phylogenetic group of taxonomically diverse organisms. In this study, we isolated diverse fungal strains from various environmental samples obtained from freshwaters in Korea. These strains were identified by performing molecular phylogenetic analyses of rDNA and/or other sequences (beta-tubulin, RNA polymerase II, and translation elongation factor 1). In addition, we examined their morphological characteristics microscopically and cultural characteristics using different media. We identified eleven unrecorded Pezizomycotina species: Cladosporium angulosum, Pseudorobillarda phragmitis, Paraconiothyrium estuarinum, Pseudopithomyces palmicola, Pyrenochaetopsis paucisetosa, Thelebolus globosus, Plagiostoma mejianum, Trichoderma cremeum, Fusarium tanahbumbuense, Coniochaeta endophytica, and Chaetomium tenue. Environmental samples obtained from different freshwater ecosystems in Korea could thus be a good source for isolating and investigating novel fungal species.

Antimicrobial active clones from soil metagenomic library

  • H. K. Lim;Lee, E. H;Kim, J.C.;Park, G. J.;K S. Jang;Park, Y. H.;K Y. Cho;S, W. Lee
    • 한국식물병리학회:학술대회논문집
    • /
    • 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
    • /
    • pp.108.1-108
    • /
    • 2003
  • Soil metagenome is untapped total microbial genome including that of the majority of unculturable bacteria present in soil. We constructed soil metagenomic library in Escherichia coli using DNA directly extracted from two different soils, pine tree rhizosphere soil and forest topsoil. Metagenomic libraries constructed from pine tree rhizosphere soil and forest topsoil consisted of approximately 33,700 clones and 112,000 clones with average insert DNA size of 35-kb, respectively. Subsequently, we screened the libraries to select clones with antimicrobial activities against Saccharomyces cerevisiae and Agrobacterium tumefaciens using double agar layer method. So far, we have a clone active against S. cerevisiae and a clone active against A. tumefaciens from the forest topsoil library. In vitro mutagenesis and DNA sequence analysis of the antifungal clone revealed the genes involved in the biosynthesis of antimicrobial secondary metabolite. Metagenomic libraries constructed in this study would be subject to search for diverse genetic resources related with useful microbial products.

  • PDF

하이브리드 데이터베이스 기반의 4단계 레이어 계층구조에서 메타규칙을 적용한 질의어 수행 모델에 관한 연구 (A Study of Query Processing Model to applied Meta Rule in 4-Level Layer based on Hybrid Databases)

  • 오염덕
    • 한국컴퓨터정보학회논문지
    • /
    • 제14권6호
    • /
    • pp.125-134
    • /
    • 2009
  • 웹을 통한 생물 데이터 접근 방식은 많은 과학자들에게 대화식으로 서로 다른 형식의 생물 데이터베이스 내용을 검색할 뿐만 아니라, 한 데이터베이스에서 다른 분자생물 데이터베이스로의 연결을 위한 강력한 도구를 제공한다. 본 논문에서의 생물 개념 모델은 생물 데이터 제어를 위한 4가지 통합 레이어를 기반으로 각 생물 데이터 소스 간의 연관성에 따른 규칙 속성을 적용하고 데이터 소스 중에 관심 대상이 되는 개체를 표현하여 하이브리드 생물 데이터 모델을 구성하였다. 특정 사용자의 응용 서비스 요구가 발생하면 해당 생물 데이터베이스와 웹 서비스를 통한 데이터 소스로부터 정보를 획득한다. 본 논문에서는 통합 레이어를 기반으로 웹 데이터 소스 상에서 정보를 탐색하기 위해 메타 규칙을 적용한 질의어 처리 모형과 수행구조를 정형화하였다.

A report of 44 unrecorded bacterial species isolated from Nakdong River in Korea

  • Ju-Hyung Jeon;Sanghwa Park;Ja Young Cho;Soo-Yeong Lee;Seoni Hwang;Jun Sung Kim;Eui-Jin Kim ; Ji Young Jung
    • 환경생물
    • /
    • 제41권3호
    • /
    • pp.308-324
    • /
    • 2023
  • This study investigated unrecorded freshwater bacterial species in Korea. Water and sediment samples were collected from the Nakdong River basin from 2020-2022. Bacterial isolates obtained through the conventional culture method with commercial media were subjected to 16S rRNA gene sequencing to identify unrecorded bacterial species. Results of 16S rRNA gene sequencing of the bacterial isolates revealed that a total of 44 bacterial isolates shared 16S rRNA gene sequence similarities of more than 98.65%, with validly published bacterial species not reported in Korea yet. These isolates were phylogenetically assigned to 4 phyla, 7 classes, 21 orders, 33 families, and 42 genera. A total of 2, 6, 12, and 24 species belonged to phyla Bacillota, Bacteroidota, Actinomycetota, and Pseudomonadota, respectively. Here, we provide details of these 44 unrecorded bacterial species, including Gram staining, colony and cellular morphologies, biochemical properties, and phylogenetic position.