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Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
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IL-18 gene expression pattern in exogenously treated AML cells

  • Seo, Min-Ji (Department of Biological Science, Sookmyung Woman's University) ;
  • Park, Min-Ha (Department of Biological Science, Sookmyung Woman's University) ;
  • Yook, Yeon-Joo (Department of Biological Science, Sookmyung Woman's University) ;
  • Kwon, Young-Sook (Department of Biological Science, Sookmyung Woman's University) ;
  • Suh, Young-Ju (Department of Biological Science, Sookmyung Woman's University) ;
  • Kim, Min-Jung (Department of Biological Science, Sookmyung Woman's University) ;
  • Cho, Dae-Ho (Department of Biological Science, Sookmyung Woman's University) ;
  • Park, Jong-Hoon (Department of Biological Science, Sookmyung Woman's University)
  • Received : 2008.02.25
  • Accepted : 2008.03.12
  • Published : 2008.06.30
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Abstract

IL-18 production may enhance immune system defense against KG-1 cells ; NB4 cells, which are associated with good prognosis, do not produce IL-18. In this study, we treated KG-1 cells with IL-18 and used microarray technology to assess subsequent effects on gene expression. In UniGene-array of 7488 human genes, expression of 57 genes, including stress related genes, increased at least 2-fold, whereas expression of 48 genes decreased at least 2-fold. Following exogenous exposure of KG-1 cells to IL-18, expression of CRYGC, $NF{\kappa}BIA$ and NACA gene were monitored. The latter is a transcriptional coactivator potentiating c-Jun-mediated transcription.$NF{\kappa}BIA$ is an inhibitor of $NF{\kappa}B$, and affects growth regulation, apoptosis and hypoxic stress. Studies, such as this one, are beginning to clarify the differences between cells associated with good and bad cancer prognoses, which may ultimately assist in medical treatment for acute myeloid leukemia.

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