• Korean Journal of Ecology and Environment
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• v.40 no.2
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• pp.273-284
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• 2007

The spatial and temporal zooplankton dynamics were examined along ca. 100-km section of the middle to lower Seomjin River (without estuary dam in river mouth) and Youngsan River (with estuary dam in river mouth) systems during study periods (2004. Nov.${\sim}$2006. Aug.) based on a monthly sampling intervals. The spatial variation of zooplankton biomass at both river ecosystems was distinct. There was considerable longitudinal variation in total zooplankton abundance in Youngsan R. stretch. The increase in total zooplankton abundance were observed along the longitudinal stretch toward the estuary dam. In contrast, there were not statistically significant longitudinal differences in total zooplankton abundance in Seomjin R. stretch. In Youngsan R. stretch, average abundance of total zooplankton (average ranges: $199{\sim}817$ Ind. $L^{-1}$ at 3 sampling sites, n=20) were nearly $4{\sim}60$ fold higher than that of Seomjin R. stretch (average ranges: $12{\sim}43$ Ind. $L^{-1}$ at 4 sampling sites, n=20). Relative abundance of rotifers (over 80% of total zooplankton abundance) at the whole sampling sites in Youngsan R. stretch were Much higher than that of the Seomjin R. stretch. The most abundant rotifers were Polyarthra spp., Brachionus spp., Colurella spp., and Keratella spp. at the both river ecosystems. In Seomjin R. stretch, copepods carbon biomass sharply increased toward in river mouth (over 40% of total zooplankton carbon biomass). Average ranges of total zooplankton filtering rates for phytoplankton at both river ecosystems varied from 21.2 to 92.9 mL $L^{-1}\;D^{-1}$ in Youngsan R. stretch and from 2.1 to 2.6 mL $L^{-1}\;D^{-1}$ in Seomjin R. stretch. Considering the zooplankton filtering rates, zooplankton as grazers of phytoplankton in Youngsan R. stretch seemed to play the more important role in planktonic food web than that of the Seomjin R. stretch.

• Korean Journal of Ecology and Environment
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• v.51 no.2
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• pp.149-159
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• 2018

Twenty-nine wetlands (20 river type and 9 lake type wetlands) in Gyeongsangnam-do were investigated to understand the characteristics of fish assemblages by the wetland type and to suggest management strategies. As a result, $10.3{\pm}4.8$ species were collected from river type wetlands on average (${\pm}SD$) and $9.1{\pm}4.1$ species from lake type wetlands. Thus, there was no significant difference in the number of species between them (Mann-Whitney U test, P>0.05). However, the species that constitute the fish assemblage showed statistically significant differences between the two wetland types (PERMANOVA, Pseudo-F=2.9555, P=0.007). Furthermore, the species that contribute the most to each type of fish assemblage were Zacco koreanus (river type, 28.51%) and Lepomis macrochirus (lake type, 23.21%), respectively (SIMPER). The results of the NMDS analysis using the fish assemblage by place classified the species into three groups (river type, lake type, and others). The current wetland management is only focused on endangered species, but this study shows a difference in fish assemblage by wetland type. Therefore, a management system based information on endemic species, exotic species and major contribution species should be provided. Furthermore, the classification of some types of wetlands based on the present topography was found to be ambiguous, and wetland classification using living creatures can be used as a complementary method. This study has limitations because only two types of wetlands were analyzed. Therefore, a detailed management method that can represent every type of wetland should be prepared through the research of all types of wetlands in the future.

• Journal of Life Science
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• v.24 no.10
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• pp.1110-1117
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• 2014

Tidal flats are continuously contaminated by human activities. This study assessed the bioremediation efficiency of tidal flat soil using microcosm reactors and microorganisms originating from the tidal area. We screened 135 bacterial strains that produce extracellular enzymes from the tidal area located in the North port of Incheon bay. Two bacterial strains (Pseudoalteromonas sp. and IC35 Halothiobacillus neapolitanus IC_S22) were selected and used in the microcosm reactors, which were specially designed to functionally mimic the ecological conditions of the tidal flats. Pseudoalteromonas sp. IC35 was selected based on its relatively high activity of the enzymes amylase, cellulose, lipase, and protease. Halothiobacillus neapolitanus IC_S22 was selected for oxidation of sulfur. The M1 and M2 microcosm reactors were operated by continuous feeding of seawater under the same conditions, but M2 was first inoculated with Pseudoalteromonas sp. IC35 before the seawater feeding. The initial COD in both the M1 and M2 microcosm reactors was 320 mg/l. The final COD was 21 mg/l (M1) and 7 mg/l (M2). The M3 and M4 microcosm reactors were operated by continuous feeding of seawater under the same conditions, but M4 was first inoculated with H. neapolitanus IC_S22. The initial sulfate concentration in both the M3 and M4 microcosm reactors was 660 mg/l, and the maximum sulfate concentration was 1,360 mg/l (M3) and 1,600 mg/l (M4).

• Korean Journal of Clinical Laboratory Science
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• v.49 no.1
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• pp.1-7
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• 2017

Zerumbone is a major component of the essential oil from Zingiber zerumbet Smith, which is a kind of wild ginger. In addition, various biological functions, such as liver protection, pain relief, atherosclerosis, and antimicrobial activity have been reported. It is also known to be effective in the proliferation of immune cells and the expression of cytokines. In this study, we investigated the effects of zerumbone on monocyte activation. First, it was confirmed that the proliferation of THP-1 cells was increased by zerumbone. The strongest increase in THP-1 proliferation after lipopolysaccharide treatment was observed at $5{\mu}M$ zerumbone treatment, and the increase of cell proliferation without lipopolysaccharide was the highest at $10{\mu}M$. Conversely, when treated with $50{\mu}M$ zerumbone, a rapid decrease of proliferation was observed regardless of the presence of lipopolysaccharide (LPS). The phosphorylation of signaling protein, Erk, induced by LPS was also increased by zerumbone. The strongest increase in phosphorylation was observed when treated with $50{\mu}M$ of zerumbone with reduced proliferation. The activity of transcription factor $NF-{\kappa}B$ was not significantly altered by zerumbone alone, but increased when treated with lipopolysaccharide. Furthermore, the transcription of the inflammatory cytokines $TNF-{\alpha}$ and IL-8, which are regulated by $NF-{\kappa}B$, is also increased by zerumbone. These results suggest that zerumbone can enhance the proliferation and activity of monocytes. Furthermore, it is believed that zerumbone can enhance rthe immune responses through increased monocyte activity in bacterial infections with LPS, thereby helping to treat effective bacteria.

• Animal Systematics, Evolution and Diversity
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• v.10 no.1
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• pp.97-123
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• 1994

The author devided th period of 1876-1945 into three epochs ; the Opening of Ports in 1876 -before the Political Reform in 1894 , the Political Reform- the Japanese annexation of Korea in 1910 , and the Epoch of Japanese Colony during 1910-1945. As civilization through including educational reform rised. The modern school system began to be introduced nongovernmentally and governmentally to Korea in the 1880's without any school laws. Were chronologycally established school regulation by Korea Government in 1895-1893, school laws by Korean Government under the supervision of the Japanese Residency-General of Korea in 1906-1910, and the educational laws of Korea by the Japanese Government-General of Korea in 1911-1943. In these epochs, the numbers of elementary , secondary and higher educational institutions and the numbers of pupils and students had increased slowly. Japanese had developed sonwhat primary education and secondary technical education, but it had checked extremely the Korean peoples to receive secondary liberal education and higher education, On the epoch of Japanese colony, Japanese occupied nearly half of elementary school teachers, almost of public secondary school teachers educated in Japan, and nearly all of professor educated in Japan in public and national colleges which were technical, and in one imperial university . Forty or more Korean teachers taught natural history chief at private secondary schools for Koreans , more than half of them being graduates of colleges of agriculture and forestry in Korea and Japan. The author mentioned curricula , and subjects and textbooks connected with biology of elementary, secondary and higher educational institutions. The pup8ls and students received biological knowledge through learning sciences at primary schools ; natural history (plants, animals and minerals ) at secondary schools including normal schools ; botany, zoology, genetics and major subjects related with biology such as anatomy, physiology, bacteriology, pland breeding at medical colleges and colleges of agriculture and forestry. There were no departments of biology , botany or zoology in Korea. Only seven Koreas graduated from departments of biology, botany or zoology at imperial universities in Japan. Some of them played the leading parts to develop education and researches of biology in the universities after 1945 Liberation.

• Journal of Dairy Science and Biotechnology
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• v.29 no.1
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• pp.23-31
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• 2011

Domestic dairy industry is now standing at the crossroad for planning next fifty years, mainly because economic and environmental situations surrounding Korean peninsula are fast changing. For the aspects of dairy consumption, fresh milk consumed less, while consumption of the other milk and dairy products is slightly increasing every year. In 2010, it is approximately estimated that 1,939,000 tons of raw milk was used and the supply would be short by about 35,000 tons, based on the amounts in the previous year. Currently, multilateral negotiations against US and EU are underway. When it will be in effect in the future, significant damage would be expected in the dairy and livestock sectors, leading to cut domestic milk supply. Quality of farm-gate milk is graded as 1A on average 90% or more, loaded with very low in microbial and somatic cell counts. Therefore, policy implications have to be placed toward switch currently the UHT processing method to Pasteurization or the LTLT technology, by which natural flavors and nutrients in milk mostly remain after heat treatment. Domestic cheese products comprise only 10% and the rest is occupied by the various kinds of imported natural products. The market size keeps increasing up to 65,423,000 tons last year. When it comes to vitalization of our natural cheese industry, cheese whey, which is a main by-product in cheese manufacture, is a critical issue to be solved and also "On-Farm Processing" would be combined with a growth of big dairy companies when few immediate issues among the relevant regulations will be eased and alleviated in the near future. Fermented milk market is recorded as a single area of gradual increase in the past 10 years, Korea. Fermented yogurts with health claims targeted stomach, liver, and intestine are popular and has grown fast in sales amounts. In this context, researches on beneficial probiotic lactic acid bacteria are one of the important projects for domestic milk and dairy industries. Labelling regulations on efficacy or health-promoting effects of functional dairy products, which is the most important issue facing domestic dairy processors, should be urgently examined toward commercial expression of the functionality by lawful means. Colostrum, a nutrition-rich yellowish fluid, is roaded with immune, growth and tissue repair factors. Bovine colostrum, a raw material for immune milk preparations and infant formula, can be used to treat or prevent infections of the gastrointestinal tract. Nanotechnology can be applied to develop new milk and dairy products such as micro-encapsulated lactase milk for consumers suffering lactose intolerance. Raw milk is suggested to be managed by its usage in the processing line because imbalance of supply and demand is structural problem in every country and thus the usage systems as in the advanced dairy countries is worth of bench-marking to stabilize milk supply and demand. Raw milk produced is desirable to divide into the three parts; domestic, import, and buffering purposes. It is strongly recommended that a domestic dairy control center as an institutional framework should be urgently established as is Dairy Board in New Zealand and Australia. Lastly, government policy should be directed to foster the highly-educated people who are majoring in Dairy Sciences or working in the dairy industry by means of financial support in studying and training abroad as well.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.34 no.5
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• pp.445-456
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• 2001

In the 1990s, Cochlodinium polykikoides red tide has been annually occurred in the southern coast of Korea and caused the mass damage to the fisheries with a huge amount of economic loss. The present study was done to establish the biological foundation for the elucidation of the mechanism of C. polykikoiaes red tide. The growth response of C. polykikoides to physico-chemical factors such as temperature, salinity, pH, and light intensity were examined using axenic cultures to evaluate the relative importance of these factors on the dynamics of natural populations, It was found that the highest growth conditions were $25^{\circ}C,\;40\%_{\circ}$, pH 7.5, and 7,500 lux, respectively. The tolerable salinity range of growth was relatively wide at an optimum temperature and was reduced to a much narrower range at a sub-optimum temperature. These findings indicate that C. polykikoides is an eurythermal and euryhaline organism. The organism demanded higher light intensity and oceanic pH narrow in its growth. C. polykikoides utilize inorganic nutrients, such as nitrate and ammonium as N, and phosphate as P. The nutritional requirements of C. polykikoides were $40{\mu}M$ for nitrate, $50{\mu}M$ for ammonium, and $5{\mu}M$ for phosphate. The half saturation constant (Ks) for growth was $2.10{\mu}M$ for nitrate, $1.03{\mu}M$ for ammonium, and $0.57{\mu}M$ for phosphate. These values were comparatively smaller than those of other dinoflagellates reported previously. We confirmed that the organism is characterized as an eutrophic species. However, ammonium Ks value is smaller than that of other eutrophic species, This result indicates that C. polykikoides red tide may outbreak in the waters which eutrophication is in progress rather than eutrophicated waters. C. polykikoides preferred ammonium better than nitrate as a nitrogen source when in a growth stage, Therefore, our results indicate that ammonium is more important nutrient on the growth of the organism in comparison with other inorganic nutrients and C. polykikoides red tide is related with the increased ammonium concentration in the coastal waters.

• Development and Reproduction
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• v.12 no.1
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• pp.57-66
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• 2008

Changes in luteinizing hormone (LH), serum testosterone (T), and salivary T levels with age were examined in Korean men. Serum was obtained from 167 Korean men of different ages ($20{\sim}69\;y$), and the serum LH and T levels were measured. Saliva samples were also obtained, and the salivary T level was determined. The LH levels did not change considerably until 40 y of age (20s, $2.5{\pm}1.0$; 30s, $2.7{\pm}1.5$; and 40s, $2.5{\pm}1.8\;mIU/mL$) but increased significantly around 50 y (50s, $3.7{\pm}1.8$ and 60s, $3.1{\pm}1.7\;mIU/mL$). Further, the serum T levels also did not change until 40 y of age (20s, $5.3{\pm}2.6$, 30s, $4.4{\pm}1.4$, 40s, $4.1{\pm}1.5\;ng/mL$) but decreased significantly at 50 y (50s, $3.4{\pm}1.5$; 60s, $2.6{\pm}0.8\;ng/mL$). The salivary T levels also showed small changes until the age of 40 y ($20s{\sim}40s$, $0.11{\pm}0.015\;ng/mL$) but decreased significantly at 50 y ($0.08{\pm}0.03\;ng/mL$). Thus, the relative ratio of salivary T to serum T levels did not change significantly in all the ages examined ($2.4{\pm}0.9%$). Linear regression analysis predicted that the LH levels increased 1.5%/y while the serum and salivary T levels decreased 1%/y and 0.8%/y, respectively. The serum T/LH ratio did not change considerably until the age of 40 y ($20s{\sim}40s$, $2.27{\pm}0.14$) but decreased significantly ($1.2{\pm}1.0$) at 50 y. Age-related changes in the salivary T/LH ratio were very similar to those in the serum T/LH ratio. These results demonstrated that LH and T levels in serum or saliva did not change considerably until 40 y of age; instead, in Korean men, from 50 y of age, the LH level increased, while the T level decreased. This suggests that primary testicular failure that occurred due to aging (approximately 50 y) and caused this phenomenon. The present study also shows that the salivary T level can be an indicator of the free T level in serum although the salivary T level correlates weakly with the total T level in serum (r=0.53). Thus, information regarding salivary T levels may be useful for studying the age-related changes occurring in male testicular physiology.

• Journal of the Korean Applied Science and Technology
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• v.34 no.3
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• pp.657-665
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• 2017

This study is for checking the possibility of Lotus Rhizome and node of Lotus Rhizome as cosmetic materials. For this we carried out biological active evaluation about anti-oxidant, anti-inflammatory and anti-wrinkle by using ethanol extract of Lotus Rhizome and node of Lotus Rhizome. We extracted Lotus Rhizome and node of Lotus Rhizome with 95% ethanol and then in order to evaluate anti-oxidant activity we treated samples by concentrations (100, 500, 1000) ${\mu}g/mL$ and carried out 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging and The activity of 2, 2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) cation radical scavenging. Also, in order to evaluate effect of anti-wrinkle we carried out evaluation of Elastase inhibitory activity. To evaluate effect of anti-inflammatory we evaluated toxicity of samples through MTT assay with a macrophage (Raw 264.7 cells) and measured nitric oxide production inhibitory activity. As a result, DPPH radical scavenging activity of Lotus Rhizome and node of Lotus Rhizome at $1000{\mu}g/mL$ was 66.7% and 99.5%, respectively and ABTS + radical scavenging activity was 51.2% and 98.3% at the same concentration, respectively. Elastase inhibitory activity results showed that the nodes of the Lotus Rhizom extract excellent anti-wrinkle efficacy than Lotus Rhizom. Node of Lotus Rhizome showed higher anti-wrinkle activity than the positive the control group BHT at $1000{\mu}g/mL$ concentration. According to the result of nitric oxide production inhibitory activity, Lotus Rhizom showed 55.8% effect and nodes of the Lotus Rhizom showed 66.6% effect respectively. This showed that effect of anti-inflammatory was greater in nodes of the Lotus Rhizom extracts. As a result it suggests that Lotus Rhizome and node of Lotus Rhizome extracts can be used as natural substance of cosmetics which are safe in antioxidant, anti-inflammatory, and anti-wrinkle.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.31 no.2
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• pp.259-266
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• 1998

A study on the biological and chemical characteristics in the middle last Sea of Korea was carried out at 31 stations in October $11\~18$, 1995 on board the R/V Tam-Yang. The chlorophyll a concentration, new and regenerated production, and the vertical diffusion of nitrate from the thermocline structure were investigated. From the vertical distribution of chlorophyll a, subsurface maxima were observed near the thermorline at most stations including the frontal zone, except at the southern stations where the maximum chloropyll a concentration occurred at the surface, The nanophytoplankton was the most dominant fraction comprising $83.5\%$ of total phytoplankton cell numbers, but netphytoplankton were common at the southern stations where the dominant species were Rhizosolenia sp. Nitrogenous new production and regenerated productions were measured using the stable isotope $^{15}N$ nitrate and ammonia uptake method. The vertically integrated nitrogen production varied between 8.470 and $72.945\;mg\;N\;m^{-2}\;d^{-1}$. The f-ratio, which is the traction of new production from primary production, waried between 0.03 and 0.72, indicating that $3\%$ to $72\%$ of primary production was supported by the input of nutrients from below the euphotic zone and the rest are supported by ammonia recycled within the euphotic layer. This range of f-ratio encompasses from extremely oligotrophic to eutrophic area characteristics. The differences in productivity and f-ratio among stations were related to frontal structure and the bottom topography. The values were high near the frontal zone and low outside of it, and the station near Ulleng Island showed the highest f-ratio. Vertical diffusion coefficients were calculated from both the water column stability (Kz-1) of King and Devol's equation (1979) and new nitrogen requirement (Kz-2). The values of Kz-2 ($0.11\~0.55\;cm^2/s$) were relatively low compared to the values reported previously.