Browse > Article
http://dx.doi.org/10.5352/JLS.2014.24.10.1110

A Study Bioremediation of Tidal Flat by Microorganism in Pilot Scale Test  

Choi, Hye Jin (Incheon Research Institute of Public Health and Environment)
Han, Young Sun (Incheon Research Institute of Public Health and Environment)
Park, Doo Hyun (Department of Chemical and Biological engineering, Seokyeong University)
Oh, Bo Young (Incheon Research Institute of Public Health and Environment)
Hur, Myung Je (Incheon Research Institute of Public Health and Environment)
Jo, Nam-Gyu (Incheon Research Institute of Public Health and Environment)
Kim, Young Hee (Incheon Research Institute of Public Health and Environment)
Kim, Jong-Guk (Department of Life Sciences and Biotechnology, Kyungpook National University)
Publication Information
Journal of Life Science / v.24, no.10, 2014 , pp. 1110-1117 More about this Journal
Abstract
Tidal flats are continuously contaminated by human activities. This study assessed the bioremediation efficiency of tidal flat soil using microcosm reactors and microorganisms originating from the tidal area. We screened 135 bacterial strains that produce extracellular enzymes from the tidal area located in the North port of Incheon bay. Two bacterial strains (Pseudoalteromonas sp. and IC35 Halothiobacillus neapolitanus IC_S22) were selected and used in the microcosm reactors, which were specially designed to functionally mimic the ecological conditions of the tidal flats. Pseudoalteromonas sp. IC35 was selected based on its relatively high activity of the enzymes amylase, cellulose, lipase, and protease. Halothiobacillus neapolitanus IC_S22 was selected for oxidation of sulfur. The M1 and M2 microcosm reactors were operated by continuous feeding of seawater under the same conditions, but M2 was first inoculated with Pseudoalteromonas sp. IC35 before the seawater feeding. The initial COD in both the M1 and M2 microcosm reactors was 320 mg/l. The final COD was 21 mg/l (M1) and 7 mg/l (M2). The M3 and M4 microcosm reactors were operated by continuous feeding of seawater under the same conditions, but M4 was first inoculated with H. neapolitanus IC_S22. The initial sulfate concentration in both the M3 and M4 microcosm reactors was 660 mg/l, and the maximum sulfate concentration was 1,360 mg/l (M3) and 1,600 mg/l (M4).
Keywords
Bioremediation; Halothiobacillus neapolitanus IC_S22; microcosm reactor; Pseudoalteromonas sp. IC35; tidal flat soil;
Citations & Related Records
Times Cited By KSCI : 5  (Citation Analysis)
연도 인용수 순위
1 Kang, D. O. and Suh, H. H. 2011. Isolation of microorganisms and Development of Microbial Augmentation for treatment. J Life Sci 21, 554-560.   DOI   ScienceOn
2 Kim, S. Y. 2006. The coastal environment change by the reclamation of tidal flats in Incheon city, west coast of central Korea. M.S, Korea National University of Education, Chung-Buk.
3 Lee, S. W., Je, J. G. and Lee, H. S. 2003. Tidal flat aspects of yellow sea area and conservation for migratory birds. Korean J Env Eco 17, 295-303.
4 Miller, G. L., Blum, R., Gennon, N. E. and Burton, A. L. 1960. Measurement of carboxymethyl celluase activity. Anal Biochem 2, 127-132.
5 Lowry, O. H., Rosenbrough, N. J. and Randall, R. J. 1951. Protein measurement with the folin phenol reagent. J Biol Chem 193, 265-275.
6 Margesin, R. and Schinner, F. 2001. Biodegradation and bioremediation of hydrocarbons in extreme envrionments. Appl Microbiol Biogechnol 56, 650-663.   DOI   ScienceOn
7 Martinez, J., Smith, D. C., Steward, G. F. and Azam, F. 1996. Variability in ectohydrolytic enzyme activities of pelagic marine bacteria and its significance for substrate repressing in the sea. Auat Microb Ecol 10, 223-230.   DOI   ScienceOn
8 Prince, R. C. 1993. Petroleum spill bioremediation in marine envrionments. Crit Rev Microbiol 19, 217-242.   DOI   ScienceOn
9 Rao, M. B., Tanksale, A. M., Ghatge, M. S. and Desphande, V. V. 1998. Molecular and biotechnological aspects of microbial protease. Microbiol Mol Biol Rev 62, 597-635.
10 Schinner, F. and Mersi, W. 1990. Xylanase, cm-cellulase and invertase activity in soil an improved method. Soil Biol Biochem 22, 511-515.   DOI   ScienceOn
11 Skovhus, T. L., Ramsing, N. B., Holmstrom, C., Kjelleberg, S. and Dahllof, I. 2004. Real-time quantitative PCR for assessment of abundance of Pseudoalteromonas species in marine samples. App Environ Microbiol 70, 2373-2382.   DOI   ScienceOn
12 Veith, A., Botelho, H., Kindinger, F., Gomes, C. M. and Kletzin, A. 2012. The sulfur oxygenase reductase from the mesophilic bacterium Halothiobacillus neapolitanus is a high active thermozyme. J Bacteriol 194, 677-685.   DOI   ScienceOn
13 You, S. J. and Kim, J. G. 1991. Evaluationon of the purification capacity of pollutants in tidal flat. J Korean Fish Soc 32, 409-415.
14 Higuchi, R., Fockler, C., Dollinger, G. and Waston, R. 1993. Kinetic PCR analysis: real monitoring of DNA amplification reactions. Biotechnology 20, 1026-1030.
15 Bae, H. J., Cho, D. C. and Kwon, S. H. 2010. Enviro-chemical changes in shoreline sediment by MgO2 for enhancement of ladigenous microbial activity. J Envrion Sci Int 9, 617-625.
16 Bulow, L. and Mosbach, K. 1987. The expression in E. coli of a polymeric gene coding for and esterase mimic catalyzing the hydrolysis of p-nitrophenyl esters. FEBS Lett 210, 147-152.   DOI   ScienceOn
17 Carling, P. A. 1982. Temporal and spatial variation in inter tidal sedimentation rates. Sedimentology 29, 17-23.   DOI
18 Capone, D. G. and Kiene, R. P. 1988. Comparison of microbial dynamics in marine and freshwater sediments: Contrasts in anaerobic carbon catabolism. Limnol Oceanogr 33, 725-749.   DOI   ScienceOn
19 Choi, D. C., Bae, H. J. and Kwon, S. H. 2012. A field research on mud flat remediation by biological treatment. JKAIS 13, 3285-3294.
20 Choi, S. B. 2010. Isolation and identification of microorganism degradable organic compounds on seaside sediment. Ph. D. Dissertation. Konkuk University, Seoul.
21 Choi, Y. J., Kim, S. H., Gu, M. J., Hoe, H. N., Kim, D. U., Cho, S. B., Kim, S. K., Jeon, C. O., Bae, G. S. and Lee, S. S. 2010. Quantitative real time PCR Using Lactobacilli as livestock probiotics. J Life Sci 20, 1896-1901.   DOI   ScienceOn
22 Dheilly, A., Soum, S. E., Klein, G. L., Bazire, A., Compere, C., Haras, D. and Dufour, A. 2010. Acitivity of the marine bacterium Pseudoalteromonas sp. Strain 3J6. Appl Environ Microbiol 76, 3452-3461.   DOI   ScienceOn
23 Jakob, P., Glockener, F. O., Unterholzner, S., Alfreider, A., Psenner, R. and Amann, R. 1998. Seasonal community and population dynamics of pelagic and archea in a high mountain lake. Appl Environ Microbiol 64, 4299-4306.
24 Kim, Y. J., Kim, S. K., Kwon, E. J., Baik, K. S., Kim, J. H. and Kim, H. 2007. Microbial population diversity of the mud flat in Suncheon bay based on 16S rDNA sequences and extracelluar enzyme activities. J Korean Soc Appl Biol Chem 50, 268-275.