• Journal of Ecology and Environment
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• v.42 no.4
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• pp.191-198
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• 2018

Improper management and unsanitary approaches are implemented in disposal of leachate, which has resulted in groundwater pollution at village Uruli Devachi, Pune, India. Various physico-chemical treatment methods are commercially available for leachate treatment. However, the application of biological methods viz. phytoremediation to the municipal solid waste landfill leachate has been limited. We report the remediation ability of Typha aungstifolia and Acrorus calamus that is capable of reducing hazardous constituents from the landfill leachate. After 96 h of hydraulic retention time (HRT), it was observed that T. aungstifolia-treated sample showed high reduction potential in reducing biochemical oxygen demand, chemical oxygen demand, hardness, total dissolved solids, Na, Mg, Ca and Ni whereas A. calamus showed greater reduction capacity for alkalinity, Cl, Cu, Zn and Cr. Furthermore, it was also observed that T. aungstifolia withstood longer HRT than A. calamus. In situ application of T. aungstifolia and A. calamus for remediation of landfill leachate carries a tremendous potential that needs to be further explored.

• Journal of The Korean Society of Grassland and Forage Science
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• v.38 no.4
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• pp.349-356
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• 2018

The objective of this study was to determine the effect of goat grazing on the surface water quality of the alpine grasslands. Seven sites were selected across the goat farm for water sample collection and analysis. Samples were analyzed for BOD (Biological oxygen demand), total nitrogen, total phosphorous, electrical conductivity and water turbidity. All the above-mentioned parameters remained below the standard limit of Korean government at the end site. Puddles showed higher values, but below standard, as stagnant water has lower physico-chemical properties as of flowing water. The present study clearly showed that goat grazing doesn't affect water quality in grasslands if grazing is according to carrying capacity of grassland and fertilizer application is judicious.

• Proceedings of the PSK Conference
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• 2002.10a
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• pp.241.1-241.1
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• 2002

Tolperisone hydrochloride is used as a muscle relaxant. Very few assay methods of tolperisone were reported. such as potentiometry. spectrophotometry and high performance thin layer chromatography. In addition. there is no report related to HPLC method to determine the tolperisone level in biological sample. In this study, A very sensitive reverse phase high performance liquid chromatographic (RP-HPLC) method for the determination of tolperisone HCI in plasma has been developed. (omitted)

• Current Optics and Photonics
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• v.5 no.3
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• pp.278-288
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• 2021

We propose a characterization method for the scattering property of microspheres using spectroscopic optical coherence tomography (OCT). To prove the effectiveness of the proposed method, we prepare solutions of different concentrations using microspheres ranging from 28 to 2300 nm in diameter. Time-frequency analysis is performed on the measured interference spectrum of each solution, and the resulting spectroscopic information is converted into histograms for centroid wavelengths. The histograms present a very sensitive response to changes in the concentration and size of microspheres. We classify them into three categories according to their characteristics. When the histogram of each category is replaced with the corresponding calculated value of the scattering coefficient, each category is mapped to a different scattering-coefficient region. It is expected that the proposed method could be used to investigate the optical characteristics of a biological sample from OCT images, which would be helpful for optical diagnostic and therapeutic applications.

• Journal of Species Research
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• v.11 no.3
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• pp.188-193
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• 2022

Araeosoma owstoni Mortensen, 1904, a newly recorded sea urchin, was collected from the Korea Strait by trawling during a research expedition in April 2017. The specimen was damaged and was severely peeled off on the aboral side during trawling. However, a test and the surface of the oral side of the sample were well preserved, allowing us to successfully identify it. The species was distinguished by the large and flexible test, the tiny apical section, and the interambulacra width which is twice of the ambulacra. Pedicellaria tridentate and triphyllous were presents, but tetradactyle pedicellaria was absent due to severe peeling on the aboral side. Moreover, a length of 1,212 bp sequence from mitochondrial COI gene was obtained and this sequence covered the general DNA barcoding region. The mean of interspecific divergence within A. owstoni from Korea and other eight species of Araeosoma from the GenBank was 6.8%. This value indicated that our species was clearly distinguishable from the others. Thus, the first Araeosoma species occurring in South Korea is presented in this study.

• Current Optics and Photonics
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• v.7 no.4
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• pp.463-470
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• 2023

Hydroxygraphene as a kind of functionalized graphene has important applications in composite, photoelectric and biological materials. In the present study, THz and microfluidic technologies were implemented to study the THz transmission characteristics of hydroxygraphene with different concentrations and residence times in magnetic and electric fields. The results show that the THz transmission intensity decreases with the increase in sample concentration and duration of an applied electric field, while it increases by staying longer in the magnetic field. The phenomenon is analyzed and explained in terms of hydrogen bond, conductivity and scattering characteristics. The results establish a foundation for future research on the THz absorption characteristics of liquid graphene based on microfluidic technology in different external environments. It also provides technical support for the application and development of graphene in THz devices.

• Molecules and Cells
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• v.46 no.2
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• pp.74-85
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• 2023

Single-cell research has provided a breakthrough in biology to understand heterogeneous cell groups, such as tissues and organs, in development and disease. Molecular barcoding and subsequent sequencing technology insert a single-cell barcode into isolated single cells, allowing separation cell by cell. Given that multimodal information from a cell defines precise cellular states, recent technical advances in methods focus on simultaneously extracting multimodal data recorded in different biological materials (DNA, RNA, protein, etc.). This review summarizes recently developed single-cell multiomics approaches regarding genome, epigenome, and protein profiles with the transcriptome. In particular, we focus on how to anchor or tag molecules from a cell, improve throughputs with sample multiplexing, and record lineages, and we further discuss the future developments of the technology.

• Journal of the Korean Society of Food Science and Nutrition
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• v.43 no.8
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• pp.1236-1247
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• 2014

Gamma irradiated-treatment of natural medicinal plants can be used to improve extraction transference number and for qualitative improvement of color when applied to functional material exploration. This study investigated the biological activities of Aralia elata cortex extracts upon gamma irradiation. In addition, different physical techniques [photostimulated luminescence (PSL) and thermoluminescence (TL)] were used for irradiation identification of Aralia elata cortex. In PSL analysis, non-irradiated (0 kGy) sample showed a negative result of 400 photon counts (PCs), whereas irradiated (5, 10, and 30 kGy) samples showed positive results of 90,100.00, 312,614.33, and 321,661.67 PCs, respectively. In the TL method, growth curve showed very unusual behaviors around $200^{\circ}C$ upon natural-irradiation of the non-irradiated (0 kGy) sample and around $150{\sim}250^{\circ}C$ for the irradiated (5, 10, and 30 kGy) samples. The TL ratio was 0.1 in non-irradiated samples at 0.011, whereas the values of irradiated samples (5, 10, and 30 kGy) were 0.1 at 1.105, 1.009, and 2.206, respectively. For phenolics of gamma-irradiated Aralia elata cortex, water and 50% ethanol extracts had the highest amounts, $17.30{\pm}0.40mg/g$ and $18.87{\pm}0.46mg/g$ at 10 kGy irradiation, respectively. The inhibitory activities of angiotensin-converting enzyme and xanthin oxidase were higher in both irradiated water and 50% ethanol extracts than in non-irradiated ones. For pancreatin ${\alpha}$-amylase and ${\alpha}$-glucosidase inhibitory activities, water and 50% ethanol extracts containing $200{\mu}g/mL$ of phenolics showed high inhibitory activities of 60~100% at all irradiation doses (0~30 kGy). This result confirmed that Aralia elata cortex extracts have greater anti-diabetic effects than acabose as a diabetic remedy. Gamma-irradiated Aralia elata cortex extracts are useful as a functional material with anti-diabetic effects. Thus, Aralia elata cortex extracts can be used as a functional material with various biological activities, and gamma-irradiation can be used to amplify biological activities in plants.

• Journal of Applied Biological Chemistry
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• v.62 no.2
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• pp.173-179
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• 2019

The aim of this study is to investigate the biological activities of Hericium erinaceus. 1,1-Diphenyl-2-picrylhydrazyl radical scavenging activity of H. erinaceus extract was higher than positive control. The inhibitory activities of xanthin oxidase, ${\alpha}$-glucosidase, and hyaluronidase was measured as functional food activity, and inhibitory activities on collagenase, tyrosinase, and astringent effect as beauty food activity in water and ethanol extracts from H. erinaceus. In functional food activity, xanthin oxidase inhibitory activities at $50-200{\mu}g/mL$ phenolic concentration in ethanol extracts from H. erinaceus showed inhibitory activity in dose dependent manner. ${\alpha}$-Glucosidase inhibitory activities at $50{\mu}g/mL$ phenolic concentration showed high activity of higher than 80%. Inhibitory activities on hyaluronidase as anti-inflammation factor showed inhibition effect in dose dependent manner both in water and ethanol extracts. In beauty food activity, Inhibitory activities on collagenase at $200{\mu}g/mL$ phenolic concentration in water and ethanol extracts showed high activity to 65.09 and 58.38% dose dependently. Tyrosinase inhibitory activity in water extract showed 9.4-58.24%. Astringent activity as pore shrink effect in ethanol extracts also showed a very high activity of 18.94-100%. Antimicrobial activity on pathogenic bacteria was highly effective on Staphylococcus aureus, Salmonella enteritidis, Vibrio parahaemolyticus and Escherichia coli at 2.5 mg/mL or above. Therefore, the extracts from H. erinaceus can be used as a functional food and beauty food resources and natural antimicrobial agent on pathogenic bacteria in food.

• Korean Journal of Food Science and Technology
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• v.36 no.4
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• pp.568-573
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• 2004

Effects of dry heating and pH on pasting, thermal, and textural properties of corn starch containing soy protein isolate (SPI) were investigated. Samples were prepared by drying corn starch dispersions containing 3% SPI at $40^{\circ}C$ (unheated sample) or additionally heating dried mixtures at $130^{\circ}C$ (heated sample). Pasting properties of starch samples and textural properties of starch gels were measured using Rapid Visco-Analyzer (RVA) and Texture Analyzer (TA), respectively. Effects of SPI addition and dry heating on retrogradation and microstructure of gel samples were investigated using DSC and SEM, respectively. Pasting viscosity of dry-heated samples was significantly higher than that of unheated samples, and at pH 8 compared to pH 6. Dry-heat treatment decreased fracture stress and strain of starch gels containing SPI. No significant effects of dry-heat treatment and pH were observed on retrogradation of starch gels. Addition of SPI retarded starch retrogradation and modified starch gel properties with increased nutritional value. Dry-heat treatment with SPI could be possible method for starch modification.