Molecules and Cells

Korean Society for Molecular and Cellular Biology (한국분자세포생물학회)
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Single-Cell Molecular Barcoding to Decode Multimodal Information Defining Cell States

  • Ik Soo Kim (Department of Microbiology, Gachon University College of Medicine)
  • Received : 2022.11.04
  • Accepted : 2023.01.18
  • Published : 2023.02.28
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Abstract

Single-cell research has provided a breakthrough in biology to understand heterogeneous cell groups, such as tissues and organs, in development and disease. Molecular barcoding and subsequent sequencing technology insert a single-cell barcode into isolated single cells, allowing separation cell by cell. Given that multimodal information from a cell defines precise cellular states, recent technical advances in methods focus on simultaneously extracting multimodal data recorded in different biological materials (DNA, RNA, protein, etc.). This review summarizes recently developed single-cell multiomics approaches regarding genome, epigenome, and protein profiles with the transcriptome. In particular, we focus on how to anchor or tag molecules from a cell, improve throughputs with sample multiplexing, and record lineages, and we further discuss the future developments of the technology.

Keywords

Acknowledgement

This work was supported by the National Research Foundation (NRF) of Korea (2021R1F1A104962311) and the Gachon University Research Fund (GCU-202102820001).

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