• Journal of Applied Biological Chemistry
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• 제49권2호
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• pp.65-68
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• 2006

• 한국미생물·생명공학회지
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• 제50권1호
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• pp.89-94
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• 2022

Triclosan (TCS) is an antimicrobial agent used in various human personal care products against both gram-positive and gram-negative bacteria. The purpose of this study was to evaluate the presence of TCS-resistant bacteria in sewage water in Peshawar, Khyber Pakhtunkhwa (KPK), Pakistan, for the first time. TCS-supplemented Luria Bertani (LB) agar was used to isolate TCS-tolerant bacteria. A total of 17 TCS-resistant isolates were randomly selected from a large pool of bacteria that showed growth on TCS-supplemented LB agar. Based on gram staining and physiochemical characteristics, the isolated strains were identified as Salmonella typhi (n = 6), Escherichia coli (n = 4), Citrobacter freundii (n = 4), Proteus mirabilis (n = 1), Enterobacter cloacae (n = 1), and Pseudomonas aeruginosa (n = 1). The Triclosan mean minimum inhibitory concentrations (MICs) for the isolates of Salmonella typhi, Escherichia coli, Citrobacter freundii, Proteus mirabilis, Enterobacter cloacae, and Pseudomonas aeruginosa were 23.66 ㎍ ml^-1, 18.75 ㎍ ml^-1, 42 ㎍ ml^-1, 32 ㎍ ml^-1, 64 ㎍ ml^-1, and 128 ㎍ ml^-1, respectively. The antibiogram revealed that all isolates were resistant to penicillin G (100%) and linezolid (100%), followed by ampicillin (94%), tetracycline (76%), tazobactam (76%), sulbactam/cefoperazone (64%), polymyxin PB (58%), amikacin (29.41%), aztreonam (29.41%), imipenem (5%), and gentamicin (5%). This is the first known study regarding the isolation of TCS-tolerant bacteria from sewage water in Peshawar, KPK, Pakistan. It was concluded that all the TCS-resistant isolates were multidrug resistant (MDR) gram-negative rod-shaped bacteria, mostly belonging to the Enterobacteriaceae family.

• Animal cells and systems
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• 제5권4호
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• pp.341-346
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• 2001

Hypertension is a complex disease with strong genetic influences. Essential hypertension has been shown to be associated with insulin resistance. To clarify the genetic basis of insulin resistance in Hypertension, case-control association studies were performed to examine candidate genes for insulin resistance in hypertension. Polymorphisms investigated were the BstO I polymorphism of the $\beta$3-adrenergic receptor (ADRB3) gene, the Xba I Polymorphism of the glycogen synthase (GSY) gene, the Dde I polymorphism of the protein phosphatase 1 G subuit (PP1G) gene, the BstE II polymorphism of the glucagon receptor (GCG-R) gene, the Pst 1 polymorphism of the insulin (INS) gene and the Acc I polymorphism of the glucokinase (GCK) gene. No significant differences were observed in the distribution of alleles and genotypes of the ADRB3, GSY PP1G, GCG-R, INS, and GCK genes between hypertensive and normotensive groups. Although the frequencies in each of these polymorphisms were not significantly different between essential hypertensive and normotensive individuals, our results may provide additional information for linkage analysis and associative studies of disorders in carbohydrate metabolism or in cardiovascular disease.

• Journal of Korean Biological Nursing Science
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• 제16권3호
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• pp.211-218
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• 2014

Purpose: This research was designed to investigate the effects of forensic education and autopsy attitude of nursing students. Methods: Data on autopsy attitude were determined and collected by means of a self-reporting questionnaire including 14 questions on perception of a need for an autopsy and 11 questions on resistance to autopsy, the respondents being 846 nursing students. The collected data were analyzed by means of an independent t-test with one-way ANOVA in an SPSS WIn 18.0 program. Results: The perception of a need for an autopsy was 4.06 on average and resistance to autopsy was 2.64 on average. The nursing students taking a forensic course showed a relatively higher level of perception of the need for an autopsy (t=-5.63, p <.001) than those not attending such a course (t=2.93, p <.001). Perception of the need for an autopsy and resistance to autopsy show a negative correlation (r=-.382, p=.003). Conclusion: A forensic medicine course raises the level of perception of the need for an autopsy by nursing students and reduces the level of resistance to autopsy.

• Journal of Korean Biological Nursing Science
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• 제10권2호
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• pp.131-140
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• 2008

Purpose: This study is about safety of the expressed human milk by investigating its bacterial contamination and antibiotic resistance of the microbes in the expressed milk. Methods: The data are collected from the 156 mothers and their newborn infants in NICU of U University Hospital from January 2004 to September 2005. Results: 1) The expressed human milk was contaminated by Staphylococcus epidermidis for 66.7%, Two mixed bacterial strain for 11.5%, Acinetobacter species for 8.0%, Klebsiella species for 4.6%, Staphylococcus aureus for 4.6%. 2) The microbes in the human milk had high resistance to the Antimicrobial agents: 77.5% for Penicillin-G, 66.6% for Oxacillin, and 63.7% for Cephalothin. 3) The distribution of microbes showed a significant depending on the place of the milk expression (p=.020). In particular, mixed bacterial strain was found more in the milks expressed at home than the milk expressed at the hospital. Conclusion: This study shows the importance of systematic education of feeding process in expressed milk: poor management of a breast pump, inadequate hand washing and imperfect breast cleaning explain the reasons of contamination 156 cases.

• The Plant Pathology Journal
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• 제29권2호
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• pp.174-181
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• 2013

The plant growth-promoting rhizobacterium Ochrobactrum lupini KUDC1013 elicited induced systemic resistance (ISR) in tobacco against soft rot disease caused by Pectobacterium carotovorum subsp. carotovorum. We investigated of its factors involved in ISR elicitation. To characterize the ISR determinants, KUDC1013 cell suspension, heat-treated cells, supernatant from a culture medium, crude bacterial lipopolysaccharide (LPS) and flagella were tested for their ISR activities. Both LPS and flagella from KUDC1013 were effective in ISR elicitation. Crude cell free supernatant elicited ISR and factors with the highest ISR activity were retained in the n-butanol fraction. Analysis of the ISR-active fraction revealed the metabolites, phenylacetic acid (PAA), 1-hexadecene and linoleic acid (LA), as elicitors of ISR. Treatment of tobacco with these compounds significantly decreased the soft rot disease symptoms. This is the first report on the ISR determinants by plant growth-promoting rhizobacteria (PGPR) KUDC1013 and identifying PAA, 1-hexadecene and LA as ISR-related compounds. This study shows that KUDC1013 has a great potential as biological control agent because of its multiple factors involved in induction of systemic resistance against phytopathogens.

• Journal of Applied Biological Chemistry
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• 제62권3호
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• pp.293-297
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• 2019

Leaf rust is the most widespread and destructive fungal disease, and outbreaks have always caused considerable losses in wheat yields. Thus, worldwide increases in wheat production depend on the development of leaf rust-resistant wheat varieties. In this study, we evaluated the resistance of forty Korean wheat cultivars to leaf rust at the seedling stage. Only two Korean wheats, Ol and Jonong, were resistant to leaf rust, whereas the remaining thirty-eight Korean wheats were susceptible to leaf rust. The Ol and Jonong varieties presented larger dry seed weights and higher antioxidant activity in response to leaf rust than the susceptible wheat varieties. No differences in ${\beta}$-1,3-glucanase activity or chlorophyll content between resistant and susceptible wheat varieties were observed. Overall, these results are important for the development of wheat varieties that are highly resistant to leaf rust and to understand the underlying mechanisms that confer leaf rust resistance.

• Corrosion Science and Technology
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• 제23권1호
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• pp.72-81
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• 2024

The Ti-6Al-4V alloy is widely used as an implant material due to its higher fatigue strength and strengthto-weight ratio compared to pure titanium, excellent corrosion resistance, and bone-like properties that promote osseointegration. For rapid osseointegration, the adhesion between the titanium surface and cellular biomolecules is crucial because adhesion, morphology, function, and proliferation are influenced by surface characteristics. Polymeric peptides and similar coating technologies have limited effectiveness, prompting a demand for alternative materials. There is growing interest in 2D nanomaterials, such as MoS₂, for good corrosion resistance and antibacterial, and bioactive properties. However, to coat MoS₂ thin films onto titanium, typically a low-temperature hydrothermal synthesis method is required, resulting in the synthesis of films with a toxic 1T@2H crystalline structure. In this study, through high-temperature annealing, we transformed them into a non-toxic 2H structure. The implant coating technique proposed in this study has good corrosion resistance and biocompatibility, and antibacterial properties.

• Bulletin of the Korean Chemical Society
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• 제27권9호
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• pp.1289-1292
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• 2006

Potassium(I) with 5,7-dihydroxy-6,4'-dimethoxyisoflavone-3'-sulfonate (L) assembles to K($H_2O$)L (L = 5,7-dihydroxy-6,4'-dimethoxyisoflavone-3'-sulfonate). It was characterized by single-crystal X-ray diffraction, element analysis, IR and $^1H$ NMR spectroscopy. It crystallizes in the monoclinic space group $P2_1$/n and reveals a seven-coordinate complex. Polyhedra potassium chains, C-H${\cdot}{\cdot}{\cdot}\pi$ and C-H${\cdot}{\cdot}{\cdot}$O and O-H${\cdot}{\cdot}{\cdot}$O hydrogen bonds lead K($H_2O$)L to a three-dimensional network structure. The biological activity of resistance to hypoxia was tested, and the results showed that the biological activity of resistance to hypoxia of K($H_2O$)L is as good as that of its precursor, irisolidone.

• Korean Membrane Journal
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• 제3권1호
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• pp.17-23
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• 2001

Polyethersulfone (PES) and cellulose acetate (CA) ultrafiltration (UF) membranes having different molecular weight cut-off values were prepared by immersion precipitation method by changing the compositions of easting solution. Egg white solution was concentrated to volume concentration ratio 4 in tubular UF with membranes made of moderately hydrophobic PES and hydrophilic CA. The process Parameters such as pressure and fluid velocity were controlled in order to apply successfully for egg white solution and to investigate the pressure dependency. The resistance values were measured to investigate the fouling and concentration polarization effect on membrane performance. During concen- tration of egg white solution by UF, the fouling layer appeared to contribute little resistance to flux compared to that of polarized layer.