• Journal of Microbiology and Biotechnology
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• 제17권7호
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• pp.1217-1220
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• 2007

The saprophytic fungus Ulocladium atrum Preuss is a promising biological control agent for Botrytis cinerea in greenhouse- and field-grown crops. However, despite its known potent antifungal activity, no antifungal substance has yet been reported. In an effort to characterize the antifungal substance from U. atrum, we isolated an antibiotic peptide. Based on extensive spectroscopic analyses, its structure was established as a cyclopeptolide with a high portion of N-methylated amino acids, and its $^1H$ and $^{13}C$ chemical shifts were completely assigned based on extensive 1D and 2D NMR experiments. Compound 1 exhibited potent antifungal activity against the plant pathogenic fungus Botrytis cinerea and moderate activity against Alternaria alternate and Magnaporthe grisea.

• Journal of Microbiology and Biotechnology
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• 제24권1호
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• pp.13-18
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• 2014

Bacillus subtilis JW-1 was isolated from rhizosphere soil as a potential biocontrol agent of bacterial wilt caused by Ralstonia solanacearum. Seed treatment followed by a soil drench application with this strain resulted in >80% reduction in bacterial wilt disease compared with that in the untreated control under greenhouse conditions. The antibacterial compound produced by strain JW-1 was purified by bioactivity-guided fractionation. Based on mass spectroscopy and nuclear magnetic resonance spectral data ($^1H$, $^{13}C$, $^1H-^1H$ correlation spectroscopies, rotating frame nuclear Overhauser effect spectroscopy, and heteronuclear multiple-bond correlation spectroscopy), the structure of this compound was elucidated as a cyclic lipopeptide composed of a heptapeptide (Gln-Leu-Leu-Val-Asp-Leu-Leu) bonded to a ${\beta}$-hydroxy-iso-hexadecanoic acid arranged in a lactone ring system.

• 환경위생공학
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• 제8권1호
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• pp.129-138
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• 1993

The marked spread of the cockraches of recent years has raised a great social problem in urban areas. The cockroach have to remove1 because transmit a disease to human as pest insect, but particulars are not yet reported on biological control agent for the cockroach removal. This study was tried for the first time on biological control for the cockroach removal. The obtained results were as follows : 1. The isolated were spore-forming bacillus 1098 strain in soil. The No. 109(TH 109) strain of the among spore-forming bacillus was showed the poisonous against Cockroach. 2. The biological characteristics and flagella antigenicity of the strain is similar to Bacillus thur-ingiensis subsp. indiana. 3. TH 109 strain have the delta-endotoxin of cuboid shap. 4. This delta-endotoxin of product by TH 109 strain was toxic to the cockroach(Blattella gemzanica. L).

• Journal of Species Research
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• 제5권3호
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• pp.477-482
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• 2016

Mite of the genus Hypoaspis of family Laelapidae are free-living soil predators mostly observed on the open grass field. Five species in the genus Hypoaspis were listed in Korean Catalog (NIBR, 2013) without detail information. Recent series of soil acarine biodiversity survey in Gyeongbuk and Gangwon provinces during 2009-2015 recovered a new record of Hypoaspis sardous (Canestrini, 1884) to Korean inventory from apple orchard and riparian grass land. Detailed description and morphological comparison with the related species, H. aculeifer which is well known biological control agent of horticultural crop pests were provided with identification keys.

• 한국미생물·생명공학회지
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• 제23권2호
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• pp.243-248
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• 1995

Agrochemicals for the plant-disease control are criticized severely for causing environmental pollution and residual problems, and consequently microbial disease control agents are expected to be safer and more economical for sustainable agriculture. Treatment of biological control agents to seed requires the use of effective delivery systems that allow full expression of the benefical qualities of the bioprotectant. For the activation and establishment of bioprotectant around the plant seed which are able protect the seeds and seedlings from pathogen attack, the optimal liquid coating formulation was obtained using 2% sodium carboxymethyl cellulose (binder), 20% sesame dregs (solid particulate material), and dried spore of Bacillus subtilis YBL-7 (bioprotectants, 10 mg/g of seed). Suppressive of root rot was demonstrated in pot trials with coated kidney bean (Phaseolus vulgaris L.) seeds. Coated seeds with B. subtilis YBL-7 spore in F. solani-infested soil reduced disease incidence by 85% to 90% after 30 days.

• 한국연초학회지
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• 제15권1호
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• pp.26-33
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• 1993

Effect of an avirulent bacteriocin-producing strain(ABPS) of Pseudomonas solanacearum adapted to low temperature on the control of tobacco bacterial wilt was examined under the natural field conditions. The ABPS of p. solanacearum were succeeding-cultured at gradually low temperature, 3$0^{\circ}C$ to 13$^{\circ}C$. The isolates adapted to low temperature grew faster than the wild type either in artificial media or on the tobacco rhizoplane. The control effect of one of the isolates on bacterial wilt was higher than that of the wild type when the bacterial suspension had been poured onto the tobacco rhizosphere soil on 1 day before and 15 days after transplanting to the field. It was suggested that ABPS of p. solanaceamm adapted to the low temperature, might be more effective biological control agent than the wild type.

• Applied Biological Chemistry
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• 제48권1호
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• pp.48-52
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• 2005

종자발아실험과 뿌리에서의 집락 형성실험으로 고추와 토마토의 근권 토양에서 plant growth promoting rhizobacteria(PGPR) 균주를 선발하였다. 그 중 포트실험에서 종자처리와 토양 관주처리 하였을 때 60% 이상 풋마름병 방제효과를 나타낸 SKU-78 균주에 대해서 포장에서의 방제효과 검증에 의해 생물농약으로의 개발 가능성을 확인하였으며, 생화학적 특성조사와 16S rDNA sequence 분석에 의해 SKU-78 균주를 Bacillus sp. SKU-78로 동정하였다.

• The Plant Pathology Journal
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• 제17권6호
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• pp.370.1-370
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• 2001

• Journal of Applied Biological Chemistry
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• 제55권4호
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• pp.247-252
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• 2012

본 연구는 뿌리혹선충의 화학적 방제를 대체할 수 있는 친환경 선충방제제 개발을 목적으로 닭 우모분으로부터 추출한 아미노산 생화학제(feather amino acid; FAA)의 고구마뿌리혹선충에 대한 방제효과를 검정하였다. 다양한 종류의 아미노산을 대상으로 고구마뿌리혹선충 유충 치사효과를 조사한 결과, 5종류(L-asparagine, L-aspartic acid, L-methionine, L-tyrosine, _L-cysteine)의 아미노산이 50 mM 농도에서 50% 이상의 치사율을 나타내었고, L-asparagine 50 mM에서 치사율이 94%로 가장 높았다. 닭 우모분으로부터 추출한 FAA 생화학제의 고구마뿌리혹선충 2령 유충의 치사력과 알 부화억제 효과를 조사한 결과, 1/50 농도에서 80% 이상의 유충 치사력과 74%의 알 부화 억제율을 보였다. 토마토 근권토양에 FAA 생화학제를 처리하고 60일간 재배시험을 통하여 관찰한 결과, 토양 내 유충밀도는 63%, 난낭수는 59%, 뿌리혹 형성 61%의 방제효과를 나타내었다. 이상의 결과로부터 닭 우모분으로부터 추출한 FAA 생화학제는 뿌리혹선충의 친환경 선충방제제로 활용될 수 있을 것으로 사료된다.

• Applied Biological Chemistry
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• 제48권3호
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• pp.296-300
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• 2005

Among the root colonizing and plant growth promoting bacteria isolated from the bacterial wilt suppressive soil, five strains were detected to produce siderophores by CAS agar assay. The most effective isolate, TS3-7 strain induced significant suppression of bacterial wilt disease in tomato and pepper plants. Seed treatment followed by soil drench application with this strain resulted in over 80% reduction of bacterial wilt disease compared with the control. Significant disease suppression by TS3-7 strain was related to the production of siderophore. Besides iron competition, induction of resistance of the host plant with siderophore was suggested to be another mode of action that suppress bacterial wilt, based on the lack of direct antibiosis against pathogen in vitro. According to Bergey's Manual of Systemic Bacteriology and 16S rDNA sequence data, TS3-7 stain was identified as Pseudomonas sp. TS3-7.