Browse > Article
http://dx.doi.org/10.4014/jmb.1308.08060

Characterization of an Antibiotic Produced by Bacillus subtilis JW-1 that Suppresses Ralstonia solanacearum  

Kwon, Jae Won (Department of Chemical and Biological Engineering, Seokyeong University)
Kim, Shin Duk (Department of Chemical and Biological Engineering, Seokyeong University)
Publication Information
Journal of Microbiology and Biotechnology / v.24, no.1, 2014 , pp. 13-18 More about this Journal
Abstract
Bacillus subtilis JW-1 was isolated from rhizosphere soil as a potential biocontrol agent of bacterial wilt caused by Ralstonia solanacearum. Seed treatment followed by a soil drench application with this strain resulted in >80% reduction in bacterial wilt disease compared with that in the untreated control under greenhouse conditions. The antibacterial compound produced by strain JW-1 was purified by bioactivity-guided fractionation. Based on mass spectroscopy and nuclear magnetic resonance spectral data ($^1H$, $^{13}C$, $^1H-^1H$ correlation spectroscopies, rotating frame nuclear Overhauser effect spectroscopy, and heteronuclear multiple-bond correlation spectroscopy), the structure of this compound was elucidated as a cyclic lipopeptide composed of a heptapeptide (Gln-Leu-Leu-Val-Asp-Leu-Leu) bonded to a ${\beta}$-hydroxy-iso-hexadecanoic acid arranged in a lactone ring system.
Keywords
Bacterial wilt; Ralstonia solanacearum; biocontrol agent; antibacterial compound; cyclic lipopeptide; surfactin isoform;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Ongena M, Jacques P. 2008. Bacillus lipopeptides: versatile weapons for plant disease biocontrol. Trends Microbiol. 16: 115-125.   DOI   ScienceOn
2 Peypoux F, Bonmatin JM, Labbe H, Das BC, Ptak M, Michel G. 1991. Isolation and characterization of a new variant of surfactin, the [Val7] surfactin. Eur. J. Biochem. 202: 101-106.   DOI   ScienceOn
3 Raaijmakers JM, Bruijn ID, Nybroe O, Ongena M. 2010. Natural functions of lipopeptides from Bacillus and Pseudomonas: more than surfactants and antibiotics. FEMS Microbiol. Rev. 34: 1037-1062.   DOI
4 Shoda M. 2000. Bacterial control of plant diseases. J. Biosci. Bioeng. 89: 515-521.   DOI   ScienceOn
5 Van Elsas JD, Kastelein P, Van Bekkum P, Van der Wolf JM, de Vries P, Van Overbeek LS. 2000. Survival of Ralstonia solanacearum biovar 2, the causative agent of potato brown rot in field and microcosm soils in temperate climates. Phytopathology 90: 1358-1366.   DOI   ScienceOn
6 Fravel DR. 2005. Commercialization and implementation of biocontrol. Annu. Rev. Phytopathol. 43: 337-359.   DOI   ScienceOn
7 Jourdan E, Henry G, Duby F, Dommes J, Barthelemy JP, Thonart P, et al. 2009. Insights into the defense-related events occurring in plant cells following perception of surfactin-type lipopeptide from Bacillus subtilis. Mol. Plant Microbe Int. 22: 456-468.   DOI
8 Kalinovskaya NI, Kuznetsova TA, Ivanova EP, Romanenko LA, Voinov VG, Huth F, et al. 2002. Characterization of surfactin-like cyclic depsipeptides synthesized by Bacillus pumilus from ascidian Halocynthia aurantium. Marine Biotechnol. 4: 179-188.   DOI
9 Kaneda T. 1991. Iso- and anteiso-fatty acids in bacteria: biosynthesis, function, and taxonomic significance. Microbiol. Rev. 55: 288-302.
10 Kim JT, Kim SD. 2007. Improvement of biological control against bacterial wilt by the combination of biocontrol agents with different mechanisms of action. J. Appl. Biol. Chem. 50: 136-143.
11 Kowall M, Vater J, Kluge B, Stein T, Franke P, Ziessow D. 1998. Separation and characterization of surfactin isoforms produced by Bacillus subtilis OKB 105. J. Colloid Interface Sci. 204: 1-8.   DOI   ScienceOn
12 Kracht M, Rokos H, Ozel M, Kowall M, Pauli G, Vater J. 1999. Antiviral and hemolytic activities of surfactin isoforms and their methyl ester derivatives. J. Antibiot. 52: 613-619   DOI
13 Leclère V, Marti R, Béchet M, Fickers P, Jacques P. 2006. The lipopeptides mycosubtilin and surfactin enhance spreading of Bacillus subtilis strains by their surface-active properties. Arch. Microbiol. 186: 475-483.   DOI
14 Denny TP. 2000. Ralstonia solanacearum - a plant pathogen in touch with its host. Trends Microbiol. 8: 486-489.   DOI
15 Nimura N, Kinoshita T. 1986. o-Phthalaldehyde-N-acetyl-Lcysteine as a chiral derivativatization reagent for liquid chromatographic optical resolution of amino acid enantiomers and its application to conventional amino acid analysis. J. Chromatogr. 352: 169-177.   DOI
16 Bais HP, Fall R, Vivano M. 2004. Biocontrol of Bacillus subtilis against infection of Arabidopsis roots by Pseudomonas syringae is facilitated by biofilm formation and surfactin production. Plant Physiol. 134: 307-319.   DOI   ScienceOn
17 Baumgart F, Kluge B, Ullrich C, Vater J, Ziessow D. 1991. Identification of amino acid substitutions in the lipopeptide surfactin using 2D NMR spectroscopy. Biochem. Biophys. Res. Commun. 177: 998-1005.   DOI   ScienceOn
18 Hayward AC. 1991. Biology and epidemiology of bacterial wilt caused by Pseudomonas solanacearum. Annu. Rev. Phytopath. 29: 65-87.   DOI   ScienceOn