• 한국약용작물학회지
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• 제28권5호
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• pp.331-338
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• 2020

Background: The aim of this study was to analyze the total phenol and total flavonoid contents and antioxidant activity of steam-treated leaves and rhizomes of Polygonatum sibiricum Redoute. In addition, we aimed to confirm their potential use as cosmetic materials by investigating their anti-aging and skin-whitening activity. Methods and Results: The leaves and rhizomes of P. sibiricum were treated with steam at different temperatures for different durations, and the antioxidant activity (DPPH and ABTS radical scavenging activity) and total phenol and total flavonoid contents of each sample were tested. The steam temperature and treatment duration siginificantly affected the antioxidant activity and, total phenol and total flavonoid content of the leaves and rhizome of P. sibiricum. Treating the P. sibiricum samples with steam at 120℃ for 12 h, yielded higher total phenol and total flavonoid contents. Comparatively, the samples treated with steam at 120℃ for 12 to 24 h showed significantly higher antioxidant activity. Further, the steamed samples of P. sibiricum demonstrated collagenase and tyrosinase inhibition activity, which indicated their anti-aging and skin-whitening properties. The samples steamed at 120℃ for 12 h, exhibited higher collagenase and tyrosinase inhibition activity. Conclusions: Leaves and rhizomes of P. sibiricum steamed at 120℃ for 12 h, showed highest antioxidant activity and, total phenol and total flavonoid contents than all other samples. Our results indicate the potential of using P. sibiricum as a cosmetic material by confirming its excellent anti-aging and whitening activity.

• 대한한의학방제학회지
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• 제24권4호
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• pp.271-282
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• 2016

Objective : In this study, we compared the antioxidant and anticancer properties of four multi-herbal formulas which were recorded in 'Dongeuibogam': Gilgyung-tang (GGT), Mokdanpi-tang (MDPT), Samso-eum (SSE), Samchulbobi-tang (SCBBT). Methods : We checked antioxidant properties of four multi-herbal formula through total phenolic content, radical scavenging activities, protective effects on genomic DNA oxidation. To investigate anticancer effects, we conducted MTT assay and analyzed morphologic change in A549 non-small lung cancer cells. Results : Total phenolic contents of four multi-herbal formulas were in a rich order of MDPT > SSE > GGT > SCBBT. Especially, MDPT revealed the highest activity than others in all antioxidant experiments. Our results indicated that treatment of those multi-herbal formulas induced growth retardation in A549 cells and MDPT also showed the highest anticancer effect ($IC_{50}=1.374mg/ml$) among them. Conclusions : Our data suggested that MDPT would be a powerful ingredient for lung cancer treatment.

• Nutritional Sciences
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• 제9권1호
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• pp.48-54
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• 2006

Methods have been developed to evaluate the total antioxidant capacity of foods and plasma but limitations are associated with their ability to determine precisely the contribution of lipophilic antioxidants in a lipid milieu as well as interactions among them Thus, we modified the Oxygen Radical Absorbance Capacity (ORAC) assay to determine the peroxyradical scavenging ability of both hydrophilic and lipophilic compartments in plasma The hydrophilic ORAC assay was performed in a phosphate buffer system utilizing 2,2'-azobis (2-amidinopropane) dihydrochloride as a peroxyradical generator and fluorescein as the target The lipophilic ORAC assay was carried out in a dimethylsulfoxide :butyronitrile (DMSO/BN, 9:1 v/v) system using 2,2'-azobis (2,4-dimethyl valeronitrile) as a peroxyradical generator and BODIPY C11 581/591 as the target Analyses were conducted in bovine serum supplemented with water - and lipid - soluble antioxidants and in human plasma. Albumin (0.5$\sim$5 g/dL) and uric acid (0.1$\sim$0.5 $\mu$mol/L) increased hydrophilic ORAC values in a dose-dependent fashion ($R^{2}$=0.97 and 0.98, respectively) but had no impact on lipophilic ORAC values. $\alpha$-Tocopherol (15$\sim$200 $\mu$mol/L) increased lipophilic ORAC values in a dose-dependent fashion ($R^{2}$=0.94); neither $\alpha$-tocopherol nor $\beta$-carotene had an impact on hydrophilic ORAC values. However, addition of $\beta$-carotene at physiological concentration (0.23$\sim$1.86 $\mu$mol/L), either alone or in combination with other carotenoids, had no significant impact on lipophilic ORAC values. Thus, while assays of 'total antioxidant capacity' in biological matrices would be a useful research and clinical tool, existing methods are limited by the lack of complete responsiveness to the full range of dietary antioxidants.

• Applied Biological Chemistry
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• 제32권4호
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• pp.357-361
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• 1989

두유 제조에서 얻어지는 두유박으로 밀가루 고오지를 이용하여 밀된장을 만든 결과 된장 숙성 중 환원당은 모든 시험구가 숙성 50일경까지 증가하다가 그 이후에는 감소하였고 두유박의 배합비가 많을수록 환원당의 함량이 감소하였으며 amino태 질소함량은 각 시험구 다같이 50일까지는 급격히 증가하나 그 이후에는 증가가 둔화되며 시험구별로는 숙성 초기에는 두유박의 배합비가 많을수록 amino태 질소 함량이 높았으나 숙성후기에는 두유박의 배합비가 많을수록 amino태 질소 함량이 낮았다. 총산 함량은 40일까지는 현저하게 증가하나 그 이후에는 둔화되었으며 각 시험구별로는 그 차이가 극히 적었다. 80일 숙성 된장의 색도는 두유박의 배합비가 많을수록 명도가 높으며 적색도가 약해지는 반면 황색도가 높게 나타났다. 숙성 90일 되는 된장의 관능 검사결과 콩나물 된장국은 대조구가 가장 좋고 두유박의 배합비가 많을수록 맛은 약간 떨어지나 대조구와 시험구 D 사이에만 유의차가 있을 뿐, 대조구와 시험구 A,B,C 사이에는 유의차가 없었다. 된장 그대로의 관능 검사 결과 대조구가 가장 좋은 점수를 얻었고, 대조구 A,B,C,D 순으로 낮은 점수를 얻어 두유박의 배합비가 많을수록 맛이 떨어지는 것으로 나타났으나 대조구와 시험구 A 사이, 시험구 B와 C 사이에는 유의차가 없었다.

• Applied Biological Chemistry
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• 제29권2호
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• pp.227-236
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• 1986

찹쌀 및 가루를 사용하여 담금한 고추장(시험구 A:찹쌀가루, 시험구 B: 찹쌀, 시험구 C: 찹쌀 및 가루의 혼용)의 화학성분, 효소력 및 texture를 측정한 결과는 다음과 같다. 수분, 조단백, 조지방, 적정산도, 무기성분, alcohol의 함량 및 pH는 시험구간에 차이가 없었다. 아미노태 질소함량과산성 protease 및 당화 amylase 활성은 A구가 높았고 환원당은 B구가 경시적으로 높았다. Ca, Cu, K, Mn, Mg, P, Fe의 무기성분이 각 시험구에서 검출되었고 이중 K의 함량이 가장 높았으며 Cu, Mn, Fe은 극히 미량이었다. Aspartic acid, glutamic acid, cystine, agrginine, proline등의 유리아미노산이 60일 숙성 고추장에서 양적으로 많이 검출되었고 histidine, valine의 함량은 적었으며, 유리아미노산의 총량은 A구 16.49, B구 13.68, C구 15.84mg/g으로서 A구가 가장 많았고 B구의 고추장이 가장 적었다. Texture를 측정한 결과 견고성과 부착성은 담금 직후에는 A구가, 숙성 후기에는 B구가 높았으며 응집성, 탄력성은 A구가 높았고 B구가 낮았다.

• Journal of Applied Biological Chemistry
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• 제60권4호
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• pp.357-362
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• 2017

본 연구는 새로운 기능성 화장품 소재 개발을 위해 노랑어리연꽃 추출물 및 분획물을 이용하여 섬유아세포내에서의 항노화 효과를 확인 하고자 하였다. 노랑어리연꽃 추출물 및 분획물의 CCD-986sk 세포를 이용하여 항노화 효과를 측정한 결과, EA 분획물이 UVB에 의해 증가된 ROS 발현을 50% 이상 억제하였으며, BuOH 분획물의 경우 pro-collagen mRNA 발현을 $25{\mu}g/mL$에서 50% 이상 발현을 증가시켰다. EA 분획물과 BuOH 분획물 모두 MMP-1 protein과 mRNA발현을 농도 의존적으로 저해시켰으며, BuOH 분획물의 경우 $10{\mu}g/mL$에서 40% 이상의 MMP-1 mRNA 발현 억제율을 확인하였다. 이는 노화 인자인 활성산소종의 생성 억제와 주름과 밀접한 관련이 있는 콜라겐의 합성 및 분해억제를 통해 항산화 및 주름 개선 효과가 있는 것으로 판단되었으며 특히 EA 와 BuOH 분획물의 높은 효능이 확인되었다. 따라서 노랑어리연꽃 EA 및 BuOH 분획물은 화장품 분야에서 항노화 소재로서의 활용가치가 있을 것으로 사료된다.

• Journal of Ginseng Research
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• 제40권3호
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• pp.260-268
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• 2016

Background: During the aging process, skin shows visible changes, characterized by a loss of elasticity and the appearance of wrinkles due to reduced collagen production and decreased elasticity of elastin fibers. Panax ginseng Meyer has been used as a traditional medicine for various diseases due to its wide range of biological activities including skin protective effects. Ginsenosides are the main components responsible for the biological activities of ginseng. However, the protective activities of an enzymatic preparation of red ginseng against human skin aging have not been investigated. Methods: The efficacy of an enzyme-treated powder complex of red ginseng (BG11001) in preventing human skin aging was evaluated by oral administration to 78 randomized individuals. All patients were requested to take three daily capsules containing either 750 mg of BG11001 or a placebo vehicle for 24 wk; at the end of the testing period, skin roughness, elasticity, and skin water content were measured. Results: BG11001 significantly reduced the average roughness of eye wrinkles and the Global Photo Damage Score compared with the placebo, although there were no significant differences in arithmetic roughness average between the groups. In addition, gross elasticity and net elasticity values increased, and transepidermal water loss level decreased, indicating improved skin elasticity and moisture content. Conclusion: In conclusion, enzyme-treated red ginseng extract significantly improved eye wrinkle roughness, skin elasticity, and moisture content. Moreover, enzyme-treated red ginseng extract would be useful substance as a bio-health skin care product.

• 한방안이비인후피부과학회지
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• 제34권4호
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• pp.1-11
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• 2021

Objectives : The aim of this study was to investigated the anti-aging and anti-wrinkles effects of the pinus koreaiensis seed(PKS) oil. Methods : The anti-oxidant effect was performed by beta-carotene bleaching assay and the intracellular proteome was analyzed expression of each 15 proteins by 2-D electrophoresis. And fatty acid was analysed by gas chromatography. Anti-wrinkle effect was analyzing human skin by the PRIMOS system. Results : Fatty acid analysis of PKS oil has shown oleic acid was 49.7% and linoleic acid was 34.1%. And the antioxidant effect was about 125% compared with alpha-tocoperol(0.1%) by beta carotene bleaching assay. In 2D PAGE analysis, fifteen protein changes in five mechanisms which was collagen synthesis pathway, MMPs, ECM-cell interaction, cytokine, antioxidant enzymes were analyzed. In case of anti-wrinkle effect was proved in vivo by analyzing human skin by the PRIMOS system. The analysis results of eye wrinkles for 4 weeks showed an improvement effect of over 6%. Conclusions : In this study, the amount of protein change in the five mechanism through the cell experiment and the skin anti wrinkle efficacy by the human in vivo test were investigated. As a result pinus koreaiensis seed oil by supercritical extraction could be used as a anti-aging and anti-wrinkle substance for the skin.

• 보존과학회지
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• 제38권3호
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• pp.180-191
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• 2022

목조문화재의 대부분은 외부 환경에 그대로 노출되어 있어 위치와 크기에 의해 보존에 관련하여 많은 어려움을 가지고 있다. 그중 흰개미나 곰팡이에 의해 발생하는 생물학적 피해는 큰 비용과 시간을 소모하게 한다. 생물학적 피해를 예방하기 위해 목재 보존제를 처리하는 방식을 선택하여 사용한다. 목재 보존제를 선별하여 최악의 환경조건인 온도는 60±3℃, 습도는 55±5%, 광량은 0.35 W/m²로 열화 처리를 하여 화학적 변화를 분석하였다. 열화 과정을 통해 색차변화에서는 Control 군에 비하여 목재 보존제 처리에서 그 변화량이 감소하는 것을 확인하였다. 목재 보존제의 열화 과정에서 함유된 유효성분 함량을 측정한 결과에서도 비교 대상인 Wood Keeper A에 비해 Gori22와 Bondex Preserve III가 유효성분 함량이 높은 것을 확인하였다. 실험을 통해 목재 보존제가 열화 과정에서 목재 시편의 변화에 영향을 주는 정도와 유효성분의 함량을 측정하여 유효 기간 및 처리 기간을 예측할 수 있다. 추후 다양한 목재 보존제를 갖추어 선택적으로 환경이나 지형 및 기간에 맞추어 목재 보존제를 선정하는 데 주요 평가 요인이 될 가능성을 제시하였다.

• 대한한의학방제학회지
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• 제25권2호
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• pp.179-191
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• 2017

Objectives : Increased oxidative stress by reactive oxygen species (ROS) has been suggested as a major cause of muscle fatigue. Although several studies have demonstrated the various biological properties of Sophora flavescens Aiton, Glycyrrhiza uralensis Fischer and Dictamnus dasycarpus Turcz, but the antioxidative potentials have not been clearly demonstrated. The present study was designed to investigate the protective effects of their water and ethanol extract mixtures (medicinal herbal mixtures, MHMIXs) on hydrogen peroxide ($H_2O_2$)-induced cell damage and apoptosis in C2C12 myoblasts. Methods : Cytotoxicity was assessed by an MTT assay. Quantitative evaluation of apoptosis induction and ROS production was evaluated by flow cytometry analysis. Expression levels of apoptosis regulatory and DNA-damage proteins were detected by Western blotting. Result : The inhibition of $H_2O_2$-induced cell proliferation was effectively blocked in extracts of 3: 1: 1 (EMHMIXs-1) or 2: 2: 1 (EMHMIXs-2) of S. flavescens, G. uralensis and D. dasycarpus Turcz, ethanol extracts from various complex extracts in C2C12 myoblasts. EMHMIXs-1 and EMHMIXs-2 also effectively attenuated $H_2O_2$-induced C2C12 cell apoptosis, which was associated with the restoration of the upregulation of Bad and death receptor 4, and downregulation of XIAP and cIAP-1 induced by $H_2O_2$. In addition, these herbal mixtures significantly blocked the $H_2O_2$-induced ROS generation and phosphorylation of $p-{\gamma}H2A.X$, which suggests that they can prevent $H_2O_2$-induced cellular DNA damage. Conclusions : The results suggest that EMHMIXs-1 and EMHMIXs-2 could block the DAN damage and apoptosis of C2C12 myoblasts by oxidative stress through blocking ROS generation.