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http://dx.doi.org/10.6114/jkood.2021.34.4.001

Skin Anti-aging and Anti-wrinkle Effects of Pinus koreaiensis Seed Oil  

Kim, Hyung-Mook (Dept. of Manufacturing Pharmacy, College of pharmacy, Chungbuk National University)
Kim, Tae-Jun (Dept. of Korean Medicine, Ophthalmology, Otolaryngology & Dermatology, College of Korean Medicine, Semyung University)
Im, Dong-bin (Dept. of Korean Medicine, College of Korean Medicine, Semyung University)
Ha, Sun-Bong (Dept. of Korean Medicine, College of Korean Medicine, Semyung University)
Kim, Ee-Hwa (Dept. of Korean Medicine, College of Korean Medicine, Semyung University)
Cha, Byung-Sun (Dept. of Biological Sciences, College of Natural Sciences, Ajou University)
Heo, Hyo-Jin (Applied Biotechnology Program, Graduate School, Ajou University)
Brito, Sofia (Applied Biotechnology Program, Graduate School, Ajou University)
Lee, Yong-Moon (Dept. of Manufacturing Pharmacy, College of pharmacy, Chungbuk National University)
Bin, Bum-Ho (Dept. of Biological Sciences, College of Natural Sciences, Ajou University)
Kwak, Byeong-Mun (Dept. of Cosmetic Science and Beauty Biotechnology, Semyung University)
Publication Information
The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology / v.34, no.4, 2021 , pp. 1-11 More about this Journal
Abstract
Objectives : The aim of this study was to investigated the anti-aging and anti-wrinkles effects of the pinus koreaiensis seed(PKS) oil. Methods : The anti-oxidant effect was performed by beta-carotene bleaching assay and the intracellular proteome was analyzed expression of each 15 proteins by 2-D electrophoresis. And fatty acid was analysed by gas chromatography. Anti-wrinkle effect was analyzing human skin by the PRIMOS system. Results : Fatty acid analysis of PKS oil has shown oleic acid was 49.7% and linoleic acid was 34.1%. And the antioxidant effect was about 125% compared with alpha-tocoperol(0.1%) by beta carotene bleaching assay. In 2D PAGE analysis, fifteen protein changes in five mechanisms which was collagen synthesis pathway, MMPs, ECM-cell interaction, cytokine, antioxidant enzymes were analyzed. In case of anti-wrinkle effect was proved in vivo by analyzing human skin by the PRIMOS system. The analysis results of eye wrinkles for 4 weeks showed an improvement effect of over 6%. Conclusions : In this study, the amount of protein change in the five mechanism through the cell experiment and the skin anti wrinkle efficacy by the human in vivo test were investigated. As a result pinus koreaiensis seed oil by supercritical extraction could be used as a anti-aging and anti-wrinkle substance for the skin.
Keywords
Pinus koreaiensis; Anti-oxidant; Anti-weinkle; Supercritical fluid extraction; Anti-oxidant;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
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