• Korean Journal of Plant Tissue Culture
• /
• v.22 no.6
• /
• pp.351-355
• /
• 1995

A truncated Polygalacturonase (PG) cDNA was fused in reverse orientation to the CaMV 35S promoter of the binary vector pCA643, and introduced into tomato cells by Agrobaderium - mediated transformation. Transformed cells were selected using kanamycin as select agent then regenerated into plants. After selfed, one transgenic line (T9), was germinated and grown on MS medium containing 1 mg/mL of kanamycin Genomic Southern analysis of a T9 progeny with labelled PG2 cDNA probe showed a single antisense PC fragment as well as the endogenous PG2 gene, suggesting that PC antisense gene was integrated into tomato genome. Northern blot analysis demonstrated that the antisense RNA was produced from the transgene at much tiger level than the endogenous PG2 gene. Polygalacturonase activity analysis of the fruit from transgenic plants demonstrated that the antisense transgene expression caused 4 to 60% reduction of endogenous PG activity.

• Journal of Microbiology and Biotechnology
• /
• v.15 no.1
• /
• pp.91-98
• /
• 2005

The hyoscyamine 6${\beta}$-hydroxylase (h6h) gene was introduced into the genome of Scopolia parviflora through the Agrobacterium rhizogenes binary vector system. The enzyme was expressed ally and tissue specific selectively in roots, resulting in five transgenic hairy root lines. The presence of the h6h gene in kanamycin-resistant hairy roots and its overexpression were confirmed by polymerase chain reaction (PCR), Northern blotting, and Western blotting, respectively. In the transgenic hairy root lines which constitutively expressed the H6H enzyme, hyoscyamine and scopolamine accumulated in high concentration. Among the transgenic hairy root lines that expressed the H6H enzyme, only two were more productive. The levels of tropane alkaloids in transgenic hairy root varied greatly: The best transgenic line (#5) contained 8.12 mg of scopolamine per g dry weight, which produced the compound three times more than wild-type root. These results suggest a possibility of improving the yield of tropane alkaloids in hairy root lines by genetic and metabolic engineering.

• Journal of Plant Biotechnology
• /
• v.32 no.3
• /
• pp.161-165
• /
• 2005

Agrobacterium tumefaciens-mediated cotyledonary explants transformation was used to produce transgenic cucumber. Cotyledonary explants of cucumber (c.v., Eunchim) were co-cultivated with strains Agrobaderium (LBA4404, GV3101, EHA101) containing the binary vector (pPTN289) carrying with CaMV 355 promoter-gus gene as reporter and NOS promoter-bar gene conferring resistance to glufosinate (herbicide Basta) as selectable marker. There was a significant difference in the transformation frequency depending Agrobacterium strains. The EHA101 of bacterial strains employed gave the maximum frequency (0.35%) for cucumber transformation. Histochemical gus and leaf painting assay showed that 15 individual lines were transgenic with the gus and bar gene. Southern blot analysis also revealed that the gus gene was successfully integrated into each genome of transgenic cucumber.

• Journal of the Institute of Convergence Signal Processing
• /
• v.13 no.4
• /
• pp.194-200
• /
• 2012

In this paper we analyze the code sequence generating algorism defined on $GF(2^n)$ proposed by S.Bostas and V.Kumar[7] and derive the implementation functions of code sequence generator using Boolean functions which can map the vector space $F_2^n$ of all binary vectors of length n, to the finite field with two elements $F_2$. We find the code sequence generating boolean functions based on two kinds of the primitive polynomials of degree, n=5 and n=7 from trace function. We then design and implement the code sequence generators using these functions, and produce two code sequence groups. The two groups have the period 31 and 127 and the magnitudes of out of phase(${\tau}{\neq}0$) autocorrelation and crosscorrelation functions {-9, -1, 7} and {-17, -1, 15}, satisfying the period $L=2^n-1$ and the correlation functions $R_{ij}({\tau})=\{-2^{(n+1)/2}-1,-1,2^{(n+l)/2}-1\}$ respectively. Through these results, we confirm that the code sequence generators using boolean functions are designed and implemented correctly.

• Journal of Korea Game Society
• /
• v.17 no.6
• /
• pp.113-120
• /
• 2017

With the development of virtual reality technology, in recent years, user-friendly hand gesture interface has been more studied for natural interaction with a virtual 3D object. Most earlier studies on the hand-gesture interface are using relatively simple hand gestures. In this paper, we suggest an intuitive hand gesture interface for interaction with 3D object in the virtual reality applications. For hand gesture recognition, first of all, we preprocess various hand data and classify the data through the binary decision tree. The classified data is re-sampled and converted to the chain-code, and then constructed to the hand feature data with the histograms of the chain code. Finally, the input gesture is recognized by MCSVM-based machine learning from the feature data. To test our proposed hand gesture interface we implemented a 'Virtual Block' game. Our experiments showed about 99.2% recognition ratio of 16 kinds of command gestures and more intuitive and user friendly than conventional mouse interface.

• Spatial Information Research
• /
• v.14 no.1 s.36
• /
• pp.151-161
• /
• 2006

This paper has a goal to construct monitoring web GIS system which displays maps that are results of the fire detecting algorithms using MODIS image. To design and build more efficient system, foreign fire monitoring systems using satellite image are researched and analyzed. As a result of that, the information about interfaces and services provided by them are obtained. In concretely, new logical DFD is used to do a process modelling. ArcIMS 4.0 of ESRI, IIS 5.1 of Microsoft are utilized to build the web GIS System. In the aspects of data input and transfer, a specific module, which converts a binary image to a kind of vector file, is developed to adjust raster data to the web GIS system.

• Journal of The Korean Society of Grassland and Forage Science
• /
• v.17 no.3
• /
• pp.285-292
• /
• 1997

This study was conducted to obtain the transgenic Brnssica napus plants with tobacco Apase gene using the binary vector system of Agrobacteriurn fumefociens. The results obtained were summarized as follows: A repressible acid phosphatase gene of Saccharon~yces cerevisiae, pho105 was used for screening of tobacco Apase cDNA. In order to identify Apase gene in tobacco genome, Southern blot analysis was pcrformed and the Apase gcnc may be present as a single copy, or at most two or three copies, in tobacco genome. To isolate the tobacco Apase gene, tobacco cDNA library was constructed using purifed mRNA from -Pi treated tobacco root and the plaque forming unit of the library was 2.8 x $10^5$ pfu/m${\ell}$, therefore the library might cover all expressed mRNAs. Using pho5 as a probe. tobacco Apase cDNA was cloned, and restriction mapping and Southern blot analysis of cDNA insert were revealed that the 3.6 kb cDNA contained tobacco acid phosphatase cDNA. Plasmid pGA695 -tcAPl was constructed by subcloning tobacco Apase cDNA into the Hind site of pGA695 with 35s promoter which can be expressed constitutively in plants. The Brassica napus cotyledonary petioles were cocultivated with the ,4 grobacteriunz and transferred to the selection medium. The transformed and regenerated plants were transplanted to soil medium. Southern blot analysis was done on the transformed plants, and it was confirmed that a foregin gene was stably integrated into the genonies of B. nnpus plants.

• Journal of Communications and Networks
• /
• v.18 no.2
• /
• pp.218-226
• /
• 2016

Coordination among users is an inevitable but time-consuming operation in wireless networks. It severely limit the system performance when the data rate is high. We present FC-MAC, a novel MAC protocol that can complete a contention within one contention slot over a joint frequency-code domain. When a node takes part in the contention, it generates randomly a contention vector (CV), which is a binary sequence of length equal to the number of available orthogonal frequency division multiplexing (OFDM) subcarriers. In FC-MAC, different user is assigned with a distinct signature (i.e., PN sequence). A node sends the signature at specific subcarriers and uses the sequence of the ON/OFF states of all subcarriers to indicate the chosen CV. Meanwhile, every node uses the redundant antennas to detect the CVs of other nodes. The node with the minimum CV becomes the winner. The experimental results show that, the collision probability of FC-MAC is as low as 0.05% when the network has 100 nodes. In comparison with IEEE 802.11, contention time is reduced by 50-80% and the throughput gain is up to 200%.

• Journal of Navigation and Port Research
• /
• v.39 no.4
• /
• pp.307-312
• /
• 2015

ARPA(Automatic Radar Plotting Aid) is a device to calculate CPA(closest point of approach)/TCPA(time of CPA), true course and speed of targets by vector operation of relative courses and speeds. The purpose of this study is to develop target acquisition and tracking technology for ARPA Radar implementation. After examining the previous studies, applicable algorithms and technologies were developed to be combined and basic ARPA functions were developed as a result. As for main research contents, the sequential image processing technology such as combination of grayscale conversion, gaussian smoothing, binary image conversion and labeling was deviced to achieve a proper target acquisition, and the NNS(Nearest Neighbor Search) algorithm was appllied to identify which target came from the previous image and finally Kalman Filter was used to calculate true course and speed of targets as an analysis of target behavior. Also all technologies stated above were implemented as a SW program and installed onboard, and verified the basic ARPA functions to be operable in practical use through onboard test.

• Journal of Intelligence and Information Systems
• /
• v.9 no.3
• /
• pp.81-97
• /
• 2003

In this paper, we suggest a method of robust watermarking for protection of multimedia data using the wavelet transform and artificial neural network. for the purpose of implementation, we decompose a original image using wavelet transform at level 3. After we classify transformed coefficients of other subbands using neural network except fur the lowest subband LL$_3$, we apply a calculated threshold about chosen cluster as the biggest. We used binary logo watermarks to make sure that it is true or not on behalf of the Gaussian Random Vector. Besides, we tested a method of dual watermark insertion and extraction. For the purpose of implementation, we decompose a original image using wavelet transform at level 3. After we classify transformed coefficients of other subbands using neural network except for the lowest subband LL$_3$, we apply a above mentioned watermark insert method. In the experimental results, we found that it has a good quality and robust about many attacks.