• Journal of Microbiology and Biotechnology
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• v.12 no.3
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• pp.522-525
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• 2002

A very rapid and efficient separation technique for cellular rhizobial cyclosophoraoses was developed based on fractional precipitation and partition chromatography. Cyclosophoraoses are known to function in the osmotic regulation and root nodule formation of legumes during the nitrogen fixation process. Cyclosophoraoses are produced as unbranched cyclic (1longrightarrow12)-${\beta}$-D-glucans in Agrobacterium or Rhizobium species. Recent research has shown that cyclosophoraoses can form inclusion complexation with various unstable or insoluble guest chemicals, thereby implying great potential for industrial application. Typical separation of pure cellular cyclosophoraoses has been so far carried out by several time-consuming steps, including size exclusion, anion exchange, and desalting liquid chromatographies, with a relatively poor recovery. However, the proposed method demonstrated that the successive application of fractional ethanol precipitation and one step of silica gel-based flash column chromatography was enough to simultaneously purify neutral or anionic forms of cyclosophoraoses. This novel technique is very rapid and provides a high recovery.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.20 no.2
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• pp.136-145
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• 1987

From the sea-urchin, Hemicentrotus pulcherrismus, we have purified by four column chromatographic steps for DNA polymerase $\alpha$ activity. The molecular weight of DNA polymerase u was determined to be around 137,000-138,000 by Sephadex G-200 gel filtration and SDS-polyacrylamide gel electrophoresis. The purified enzyme had the optimal activity at pH 7.4. This enzyme showed to be a function of the metal ion $K^+,\;Na^+$\;and\;Mg^{2+}$ employed as activators, the optimum $K^+$\;or\;Na^+ concentration were 20 mM or 25mM and the optimum $Mg^{2+}$ concentration was 10 mM. The enzyme activity was inhibited by N-ethyl-maleimide, aphidicolin, cytosine $\beta-D-arabinofuranoside$ 5'-triphoshate (ara CTP) and phosphonoacetic acid.

• Communications of the Korean Mathematical Society
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• v.9 no.4
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• pp.881-889
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• 1994

Let ($X, \Beta, \mu$) be a measure space with the $\sigma$-algebra $\Beta$ and the probability measure $\mu$. Throughouth this article set equalities and inclusions are understood as being so modulo measure zero sets. A transformation T defined on a probability space X is said to be measure preserving if $\mu(T^{-1}E) = \mu(E)$ for $E \in B$. It is said to be ergodic if $\mu(E) = 0$ or i whenever $T^{-1}E = E$ for $E \in B$. Consider the sequence ${x, Tx, T^2x,...}$ for $x \in X$. One may ask the following questions: What is the relative frequency of the points $T^nx$ which visit the set E\ulcorner Birkhoff Ergodic Theorem states that for an ergodic transformation T the time average $lim_{n \to \infty}(1/N)\sum^{N-1}_{n=0}{f(T^nx)}$ equals for almost every x the space average $(1/\mu(X)) \int_X f(x)d\mu(x)$. In the special case when f is the characteristic function $\chi E$ of a set E and T is ergodic we have the following formula for the frequency of visits of T-iterates to E : $$ lim_{N \to \infty} \frac{$\mid${n : T^n x \in E, 0 \leq n $\mid$}{N} = \mu(E) $$ for almost all $x \in X$ where $$\mid$\cdot$\mid$$ denotes cardinality of a set. For the details, see [8], [10].

• Journal of the Korean Mathematical Society
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• v.54 no.2
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• pp.599-612
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• 2017

If ${\psi}$ is analytic on the open unit disk $\mathbb{D}$ and ${\varphi}$ is an analytic self-map of $\mathbb{D}$, the weighted composition operator $C_{{\psi},{\varphi}}$ is defined by $C_{{\psi},{\varphi}}f(z)={\psi}(z)f({\varphi}(z))$, when f is analytic on $\mathbb{D}$. In this paper, we study normal, cohyponormal, hyponormal and normaloid weighted composition operators on the Hardy and weighted Bergman spaces. First, for some weighted Hardy spaces $H^2({\beta})$, we prove that if $C_{{\psi},{\varphi}}$ is cohyponormal on $H^2({\beta})$, then ${\psi}$ never vanishes on $\mathbb{D}$ and ${\varphi}$ is univalent, when ${\psi}{\not\equiv}0$ and ${\varphi}$ is not a constant function. Moreover, for ${\psi}=K_a$, where |a| < 1, we investigate normal, cohyponormal and hyponormal weighted composition operators $C_{{\psi},{\varphi}}$. After that, for ${\varphi}$ which is a hyperbolic or parabolic automorphism, we characterize all normal weighted composition operators $C_{{\psi},{\varphi}}$, when ${\psi}{\not\equiv}0$ and ${\psi}$ is analytic on $\bar{\mathbb{D}}$. Finally, we find all normal weighted composition operators which are bounded below.

• Biomedical Science Letters
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• v.12 no.3
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• pp.139-143
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• 2006

Jopock (Jpk) has previously been ascertained that induces both bacterial and mammalian cell death. The Escherichia coli cells expressing Glutathion S-transferase (GST) fused Jpk showed elongated phenotype and inhibited cell growth which led eventual cell death. In an attempt to search the genetic suppressor of the lethal protein Jpk in bacterial cells, we constructed a unidirectional protein expression vector inserting tac promoter next to the C-terminus Jpk in pGEX-Jpk. The function of additional tac promoter was confirmed by substituting lac promoter in Plac-TOPO plasmid. The cells harboring plac- TOPO, which regulates $lacZ{\alpha}$ gene expression under lac promoter, formed blue colonies in 5-bromo-4-3 $indolyo-{\beta}-D-galactoside$ (X-gal) plate. When lac promoter was changed to tac promoter, same results were observed. Since the addition of tac promoter did not affect the toxic effect of Jpk, the pGEX-Jpk-ptac could be a useful vector for the screening of suppressor(s) for Jpk, in which GST-Jpk and a putative Jpk-suppressing protein are coexpressing from two unidirectional tac promoters, which response to the same inducer, $isopropyl-{\beta}-D-thiogalactopyranoside (IPTG)$.

• The Journal of Korea CHUNA Manual Medicine
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• v.1 no.1
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• pp.125-137
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• 2000

the aim of this study was to investigate whether osteoporosis can be advanced after cerebral infarction Of not and role of female sex hormone. all animals were classified 4 groups; group of ovariectomy (OVXgroup) group of cerebral infarction( INF group), group of combination ovariectomy and cerebral infarction(OVX + INF group), naturally intact group for control data (NOR group ). cerebral infarction was made by Chen,s method with some modification and ovariectomy was performed by Wayforth,s method. experimental data was collected at 15 days, 1 month, 3 months and 6months after starting observation. serum 17 ${{\beta}-estradiol\;(E_2)}$ was determined by radioimmunoassay, for comparision of osteoporosis formation, bone density, serum osteocalcin, Serum total calcium and phosphorus, Serum AST Concentration, Serum ALT Concentration, Creatinine Concentration were also calculated. we have found that cerebral ischemia decreases not only the serum concentration of 17 ${{\beta}-estradiol\;(E_2)}$ and inhibits but also the physiologically compensatory function of the ovariectomized rats and that the decreased estrogen concentration followed by cerebral infarction have not produced osteoporosis, regretfully.

• Journal of Yeungnam Medical Science
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• v.29 no.2
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• pp.77-82
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• 2012

AMP-activated protein kinase (AMPK) is an important cellular fuel sensor. Its activation requires phosphorylation at Thr-172, which resides in the activation loop of the ${\alpha}1$ and ${\alpha}2$ subunits. Several AMPK upstream kinases are capable of phosphorylating AMPK at Thr-172, including LKB1 and CaMKK${\beta}$ ($Ca^{2+}$/calmodulin-dependent protein kinase kinase${\beta}$). AMPK has been implicated in the regulation of physiological signals, such as in the inhibition of cholesterol fatty acid, and protein synthesis, and enhancement of glucose uptake and blood flow. AMPK activation also exhibits several salutary effects on the vascular function and improves vascular abnormalities. AMPK is modulated by numerous hormones and cytokines that regulate the energy balance in the whole body. These hormone and cytokines include leptin, adiponectin, ghrelin, and even thyroid hormones. Moreover, AMPK is activated by several drugs and xenobiotics. Some of these are in being clinically used to treat type 2 diabetes (e.g., metformin and thiazolidinediones), hypertension (e.g., nifedipine and losartan), and impaired blood flow (e.g., aspirin, statins, and cilostazol). I reviewed the precise mechanisms of the AMPK activation pathway and AMPK-modulating drugs.

• Korean Journal of Adult Nursing
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• v.29 no.2
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• pp.200-210
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• 2017

Purpose: The purpose of this study was to assess the relationship between menopausal symptoms and decline in cognitive functioning of menopausal women with mediating effects of health promoting behavior. Methods: Using a convenience sampling, 140 menopausal women were recruited for the cross-sectional survey. Data were collected by using the Menopause Rating Scale, Health Promoting Lifestyle Profile, Everyday Cognition, and Korean Mini-Mental State Examination. Results: The mean scores for menopausal symptoms, health promotion behavior, and subjective cognitive decline were 14.40, 153.79, and 67.40 respectively. Health promotion behavior was directly affected by menopausal symptom ($R^2=8%$). Cognitive decline was directly affected by menopausal symptom ($R^2=11%$). Menopausal symptom (${\beta}=.33$, p<.001) and health promotion behavior (${\beta}=.21$, p=.014) were found to be predictive factors in subjective cognitive decline and explained 14%. Health promotion behavior had a partial mediating effect in the relationship between menopausal symptom and perceived cognitive decline (Sobel test: Z=2.05, p=.040). Conclusion: Based on the findings of this study, developing nursing intervention programs focusing on decreasing menopausal symptoms and encouraging health promotion behavior are recommended to improve cognitive decline in menopausal women.

• The Plant Pathology Journal
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• v.21 no.3
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• pp.195-206
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• 2005

Spatial and temporal expression of pathogenesis-related (PR) gene and proteins has been recognized as inducible defense response in pepper plants. Gene expression and/or protein accumulation of PR-1, $\beta-1,3-glucanase$ and chitinase was predominantly found in pepper plants during the inoculations by Xanthomonas campestris pv. vesicatoria, Phytophthora capsici and Colletotrichum coccodes. PR-1 and chitinase genes were also induced in pepper plants in response to environmental stresses, such as high salinity and drought. PR-1 and chitinase gene expressions by biotic and abiotic stresses were regulated by their own promoter regions containing several stress-related cis-acting elements. Overexpression of pepper PR-1 or chitinase genes in heterogeneous transgenic plants showed enhanced disease resistance as well as environmental stress tolerances. In this review, we focused on the putative function of pepper PR-1, $\beta-1,3-glucanase$ and chitinase proteins and/or genes at the biochemical, molecular and cytological aspects.

• Journal of Dental Rehabilitation and Applied Science
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• v.32 no.2
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• pp.102-108
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• 2016

Headache disorders, one of most common disease in general population, have been developed according to many versions of international classifications. The primary headaches are those in which no consistently identified organic cause can be determined. It is divided into the following categories: (1) migraine, (2) tension-type headache, (3) cluster headache and other trigeminal autonomic cephalalgias, (4) other primary headaches. This review described a diagnosis of primary headache disorders based on International Classification of Headache Disorders (ICHD)-3 beta criteria.