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http://dx.doi.org/10.5423/PPJ.2005.21.3.195

Functional Characterization of PR-1 Protein, β-1,3-Glucanase and Chitinase Genes During Defense Response to Biotic and Abiotic Stresses in Capsicum annuum  

Hong, Jeum-Kyu (Division of Bioscience and Technology, College of Life and Environmental Science, Korea University)
Hwang, Byung-Kook (Division of Bioscience and Technology, College of Life and Environmental Science, Korea University)
Publication Information
The Plant Pathology Journal / v.21, no.3, 2005 , pp. 195-206 More about this Journal
Abstract
Spatial and temporal expression of pathogenesis-related (PR) gene and proteins has been recognized as inducible defense response in pepper plants. Gene expression and/or protein accumulation of PR-1, $\beta-1,3-glucanase$ and chitinase was predominantly found in pepper plants during the inoculations by Xanthomonas campestris pv. vesicatoria, Phytophthora capsici and Colletotrichum coccodes. PR-1 and chitinase genes were also induced in pepper plants in response to environmental stresses, such as high salinity and drought. PR-1 and chitinase gene expressions by biotic and abiotic stresses were regulated by their own promoter regions containing several stress-related cis-acting elements. Overexpression of pepper PR-1 or chitinase genes in heterogeneous transgenic plants showed enhanced disease resistance as well as environmental stress tolerances. In this review, we focused on the putative function of pepper PR-1, $\beta-1,3-glucanase$ and chitinase proteins and/or genes at the biochemical, molecular and cytological aspects.
Keywords
chitinase; disease resistance; environmental stress; PR-1;
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1 A-H-Mackerness, S., John, C. F., Jordan, B. and Thomas, B. 2001. Early signaling components in ultraviolet-B responses:distinct roles for different reactive species and nitric oxide. FEBS Lett. 489:237-242   DOI   PUBMED   ScienceOn
2 Breda, C., Sallaud, C., EI-Turk, J., Buffard, D., de Kozak, I., Esnault, R. and Kondorosi, A. 1996. Defense reaction in Medicago sativa: a gene encoding a class 10 PR protein is expressed in vascular bundles. Mol. Plant-Microbe Interact. 9:713-719   DOI   ScienceOn
3 Constabel, C. P. and Brisson, N. 1995. Stigma- and vascular-specific expression of the PR-10a gene of potato: a novel pattern of expression of a pathogenesis-related gene. Mol. Plant-Microbe Interact. 8:104-113   DOI
4 Do, H. M., Hong, J. K., Jung, H. W., Ham, J. H. and Hwang, B. K. 2003. Expression of peroxidase-like genes, $H_2O_2$ production and peroxidase activity during the hypersensitive response to Xanthomonas campestris pv. vesicatoria in Capsicum annuum. Mol. Plant-Microbe Interact. 16:196-205   DOI   ScienceOn
5 Eyal, Y., Meller, Y., Lev-Yadan, S. and Fluhr, R. 1993. A basic-type PR-1 promoter directs ethylene responsiveness, vascular and abscission zone-specific expression. Plant J. 4:225-234   DOI   ScienceOn
6 Hong, J. K. and Hwang, B. K. 1998. Influence of inoculum density, wetness duration, plant age, inoculation method, and cultivar resistance on infection of pepper plants by Colletotrichum coccodes. Plant Dis. 82: 1079-1083   DOI   ScienceOn
7 Hong, J. K. and Hwang, B. K. 2005a. Induction of enhanced disease resistance and oxidative stress tolerance by overexpression of pepper basic PR-1 gene in Arabidopsis. Physiol. Plant. 124:267-277   DOI   ScienceOn
8 Hong, J. K. and Hwang, B. K. 2005b. Promoter activation of pepper class II basic chitinase gene, CAChi2, and enhanced bacterial disease resistance and osmotic stress tolerance in the CAChi2-overexpressing Arabidopsis. Planta (in press)
9 Hong, J. K., Lee, S. C. and Hwang, B. K. 2005. Activation of pepper basic PR-1 gene promoter during defense signaling to pathogen, abiotic and environmental stresses. Gene 356: 169-180   DOI   PUBMED   ScienceOn
10 Hwang, B. K. 2001. Cytology, physiology and molecular genetics of resistance to Phytophthora blight in pepper plants. Plant Pathol. J. 17:9-21   과학기술학회마을   ScienceOn
11 Hwang, B. K., Sunwoo, J. Y., Kim, Y. J. and Kim, B. S. 1997. Accumulation of $\beta$-1, 3-glucanase and chitinase isoforms, and salicylic acid in the DL-$\beta$-amino-n-butyric acid-induced resistance response of pepper stems to Phytophthora capsici. Physiol. Mol. Plant Pathol. 51:305-322   DOI   ScienceOn
12 Jacinto, J., McGurl, B., Franceschi, V., Delano-Freier, J. and Ryan, C. A. 1997. Tomato prosystemin promoter confers wound-inducible, vascular bundle-specific expression of the $\beta$-glucuronidase gene in transgenic tomato plants. Planta 203 :406-412   DOI   ScienceOn
13 Kim, Y. J. and Hwang, B. K. 1997. Isolation of a basic 34 kiloDalton $\beta$-1 ,3-glucanase with inhibitory against Phytophthora capsici from pepper stems. Physiol. Mol. Plant Pathol. 50: 103-115   DOI   ScienceOn
14 Niderman, T., Genetet, I., Bruyere, T, Gees, R., Stintzi, A., Legrand, M., Fritig, B. and Mosinger, E. 1995. Pathogenesisrelated PR-1 proteins are antifungal. Plant Physiol. 108: 17-27   DOI   ScienceOn
15 Eulgem, T., Rushton, P. J., Schmelzer, E., Hahlbrock, K. and Somssich, I. E. 1999. Early nuclear events in plant defence signalling: rapid gene activation by WRKY transcription factors. EMBO J. 18:4689-4699   DOI   ScienceOn
16 Pasqualini, S., Piccioni, C., Reale, L., Ederli, L., Torre, G. D. and Ferranti, F. 2003. Ozone-induced cell death in tobacco cultivar Bel W3 plants. The role of programmed cell death in lesion formation. Plant Physiol. 133:1122-1134   DOI   ScienceOn
17 Sarowar, S., Kim, Y. J., Kim, E. N., Kim, K. D., Hwang, B. K., Islam, R. and Shin, J. S. 2005. Overexpression of a pepper basic pathogenesis-related protein 1 gene in tobacco plants enhances resistance to heavy metal and pathogen stresses Plant Cell Rep. 24:216-224   DOI   ScienceOn
18 Takeuchi, Y., Yoshikawa, M., Takeba, G., Kunisuke, T., Shibata, D. and Horino, O. 1990. Molecular cloning and ethylene induction of mRNA encoding a phytoalexin elicitor-releasing factor, $\beta$-1 ,3-endoglucanase, in soybean. Plant Physiol. 93:673-682   DOI   ScienceOn
19 Lee, S. C. and Hwang, B. K. 2005. Induction of some defense-related genes and oxidative burst is required for the establishment of systemic acquired resistance in Capsicum annuum. Planta 221 :790-800   DOI   ScienceOn
20 Linthorst, H. J. M., Meuwissen, R. L. J., Kauffinann, S. and Bol, J. F. 1989. Constitutive expression of pathogenesis-related proteins PR-1, GRP, and PR-S in tobacco has no effect on virus infection. Plant Cell 1:285-291   DOI   ScienceOn
21 Shinozaki, K., Yamaguchi-Shinozaki, K. and Seki, M. 2003. Regulatory network of gene expression in the drought and cold stress responses. Curr. Opin. Plant Biol. 6:410-417   DOI   ScienceOn
22 Lee, Y. K., Hippe-Sanwald, S., Jung, H. W., Hong, J. K., Hause, B. and Hwang, B. K. 2000a. In situ localization of chitinase mRNA and protein in compatible and incompatible interactions of pepper stems with Phytophthora capsici. Physiol. Mol. Plant Pathol. 57: 111-121   DOI   ScienceOn
23 Mithofer, A., Schulze, B. and Boland, W. 2004. Biotic and heavy metal stress response in plants: evidence for common signals. FEBS Lett. 566:1-5   DOI   PUBMED   ScienceOn
24 Punja, Z. K. and Zhang, Y. Y. 1993. Plant chitinases and their roles in resistance to fungal diseases. J. Nematol. 25:526-540
25 Wubben, J. P., Joosten, M. H. A. J., Van Kan, J. A. L. and De Wit, P. J. G M. 1992. Subcellular localization of plant chitinase and 1,3-$\beta$-glucanases in Cladosporium fulvum (syn. Fulvia fulva)infected tomato leaves. Physiol. Mol. Plant Pathol. 41:23-32   DOI
26 Rauscher, M., Adam, A. L., Wirtz, S., Guggenheim, R., Mendgen, K. and Deising, H. B. 1999. PR-1 protein inhibits the differentiation of rust infection hyphae in leaves of acquired resistant broad bean. Plant J. 19:625-633   DOI   ScienceOn
27 Sietsma, J. H., Everleigh, D. E. and Haskins, R. H. 1969. Cell wall composition and protoplast formation of some oomycete species. Biochem. Biophys. Acta 184:306-317   DOI   ScienceOn
28 van Kan, J. A., Joosten, M. H., Wagemakers, C. A., van den Berg-Velthuis, G. C. and de Wit, P. J. 1992. Differential accumulation of mRNAs encoding extracellular and intracellular PR proteins in tomato induced by virulent and avirulent races of Cladospoium fulvum. Plant Mol. Biol. 20:513-527   DOI   ScienceOn
29 Apse, M. P. and Blumwald, E. 2002. Engineering salt tolerance in plants. Curr. Opin. Biotechnol. 13:146-150   DOI   ScienceOn
30 Sutoh, K. and Yamauchi, D. 2003. Two Cis-acting elements necessary and sufficient for giberellin-upregulated proteinase expression in rice seeds. Plant J. 34:635-645   DOI   ScienceOn
31 Chen, R. D., Yu, L. X., Greer, A. F., Cheriti, H. and Tabaeizadeh, Z. 1994. Isolation of an osmotic stress- and abscisic acid-induced gene encoding an acidic endochitinase from Lycoper-sicon chilense. Mol. Gen. Genet. 245: 195-202
32 Cherif, M., Benhamou, N. and Belanger, R. 1992. Occurrence of cellulose and chitin in the hyphal walls of Pythium ultimum: a comparative study with other plant pathogenic fungi. Can. J. Microbiol. 39:213-222   DOI   ScienceOn
33 Alexander, D., Goodman, R. M., Gut-Rella, M., Glascock, C., Weymann, K., Friedrich, L., Madoz, D., Ahl-goy, P., Luntz, T., Ward, E. and Ryals, J. 1993. Increased tolerance to two oomycete pathogens in transgenic tobacco expressing pathogenesis-regulated la. Proc. Natl. Acad. Sci. U.S.A. 90:7327-7331
34 Kim, Y. J. and Hwang, B. K. 2000. Pepper gene encoding a basic pathogenesis-related 1 protein is pathogen and ethylene inducible. Physiol. Plant. 108:51-60   DOI
35 Metraux, J. P., Streit, L. and Staub, T. 1988. A pathogenesis-related protein in cucumber is a chitinase. Physiol. Mol. Plant Pathol. 33:1-9   DOI
36 Sela-Buurlage, M. B., Ponstein, A. S., Bres-Vloemans, S. A., Melchers, L. S., Van den Elzen, P. J. M. and Cornelissen, B. J. C. 1993. Only specific tobacco (Nicotiana tabacum) chitinases and $\beta$-1 ,3-glucanases exhibit antifungal activity. Plant Physiol. 101:857-863   DOI
37 Hong, J. K., Jung, H. W., Kim, Y. J. and Hwang, B. K. 2000. Pepper gene encoding a basic class II chitinase is inducible by pathogen and ethephon. Plant Sci. 159:39-49   DOI   ScienceOn
38 van Loon, L. C. and van Strien, E. A. 1999. The families of pathogenesis-related proteins, their activities, and comparative analysis of PR-1 type proteins. Physiol. Mol. Plant Pathol. 55:85-97   DOI   ScienceOn
39 Hoegen, E., Stromberg, A., Pihlgren, U. and Kombrink, E. 2002. Primary structure and tissue-specific expression of the pathogenesis-related protein PR-1b in potato. Mol. Plant Pathol. 3:329-345   DOI   ScienceOn
40 Apel, K. and Hirt, H. 2004. Reactive oxygen species: metabolism, oxidative stress, and signal transduction. Annu. Rev. Plant Biol. 55:373-99   DOI   PUBMED   ScienceOn
41 Kuzniak, E. and Sklodowska, M. 2005. Fungal pathogen-induced changes in the antioxidant systems of leaf peroxisomes from infected tomato plants. Planta (in press)
42 Keen, N. T. and Yoshikawa, M. 1983. $\beta$-1,3-Endoglucanase from soybean releases elicitor-active carbohydrates from fungus cell wall. Plant Physiol. 71:460-465   DOI   ScienceOn
43 Broglie, K., Chet, I., Holliday, M., Cressman, R., Biddle, P., Knowlton, S., Mauvais, C. J. and Broglie, R. 1991. Transgenic plants with enhanced resistance to the fungal pathogen Rhizoctonia solani. Science 254:1194-1197   DOI   PUBMED   ScienceOn
44 Ernst, D., Schraudner, M., Langebartels, C. and Sandermann, H. Jr. 1992. Ozone-induced changes of mRNA levels of $\beta$-I ,3-glucanase, chitinase and 'pathogenesis-related' protein 1b in tobacco plants. Plant Mol. Biol. 20:673-682   DOI   ScienceOn
45 Abe, H., Yamaguchi-Shinozaki, K., Urao, T., Iwasaki, T., Hosokawa, D. and Shinozaki K. 1997. Role of arabidopsis MYC and MYB homologs in drought-and abscisic acid-regulated gene expression. Plant Cell 10: 1859-1868
46 Hong, J. K and Hwang, B. K. 2002. Induction by pathogen, salt and drought of a basic class II chitinase mRNA and in situ localization in pepper (Capsicum annuum). Physiol. Plant. 114:549-558   DOI   ScienceOn
47 Eulgem, T., Rushton, P. J., Robatzek, S. and Somssich, I. E. 2000. The WRKY superfamily of plant transcription factors. Trends Plant Sci. 5:199-206   DOI   ScienceOn
48 Jung, H. W. and Hwang, B. K. 2000b. Pepper gene encoding a basic $\beta$-1 ,3-glucanase is differentially expressed in pepper tissues upon pathogen infection and ethephon or methyl jasmonate. Plant Sci. 159:97-106   DOI   ScienceOn
49 Kim, Y. J. and Hwang, B. K. 1994. Differential accumulation of $\beta$-1 ,3-glucanase and chitinase isoforms in pepper stems infected by compatible and incompatible isolates of Phytophthora capsici. Physiol. Mol. Plant Pathol. 45: 195-209   DOI   ScienceOn
50 Kim, Y. J. and Hwang, B. K. 1996. Purification, N-terminal amino acid sequencing and antifungal activity of chitinases from pepper stems treated with mercuric chloride. Physiol. Mol. Plant Pathol. 48:417-432   DOI   ScienceOn
51 Wu, H., Michler, C. H., LaRussa, L. and Davis, J. M. 1999. The pine Pschi4 promoter directs wound-induced transcription. Plant Sci. 142:199-207   DOI   ScienceOn
52 Cutt. J. R., Harpster, M. H., Dixon, D. C., Carr, J. P., Dunsmuir, P. and Klessig, D. F. 1989. Disease response to tobacco mosaic virus in transgenic tobacco plants that constitutively express the pathogenesis-related PR1b gene. Virology 173:89-97   DOI   ScienceOn
53 Lee, Y. K., Hippe-Sanwald, S., Lee, S. C., Hohenberg, H. and Hwang, B. K. 2000b. In situ localization of PR-1 mRNA and PR-1 protein in compatible and incompatible interactions of pepper stems with Phytophthora capsici. Protoplasma 211:64-75   DOI   ScienceOn
54 Jung, H. W and Hwang, B. K. 2000a. Isolation, partial sequencing, and expressing of pathogenesis-related cDNA genes from pepper leaves infected by Xanthomonas campestris pv. vesicatoria. Mol. Plant-Microbe Interact. 13:136-142   DOI   ScienceOn
55 Nielsen, K. K., Mikkelsen, J. D., Kragh, K. M. and Bojsen, K. 1993. An acidic class III chitinase in sugar beet: Induction by Cercospora beticola, characterization, and expression in transgenic tobacco plants. Mol. Plant-Microbe Interact. 6:495-506   DOI   ScienceOn
56 Finkelstein, R. R., Gampala, S. S. L. and Rock, C. D. 2002. Abscisic acid signaling in seeds and seedlings. Plant Cell S15-S45
57 Daugrois, J. H., Lafiette, C., Barthe, J. P. and Touze, A. 1990. Induction of $\beta$-I ,3-glucanase and chitinase activity in compatible and incompatible interactions between Colletotrichum linthemuthianum and bean cultivars. J. Phytopathol. 130:225-234   DOI
58 Lee, Y. K. and Hwang, B. K. 1996. Differential induction and accumulation of $\beta$-1 ,3-glucanase and chitinase isoforms in the intercellular space and leaf tissues of pepper by Xanthomonas campestris pv. vesicatoria. J. Phytopathol. 144:79-87   DOI
59 Mauch, F., Mauch-Mani B. and Boller, T. 1988. Antifungal hydrolases in pea tissue. Plant Physiol. 88:936-942   DOI   ScienceOn