• 생명과학회지
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• 제15권3호
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• pp.423-426
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• 2005

운향과(Rutaceae)인 황백나무(Phellodendron nmurense Rupr.)의 줄기 점 질에서 2종의 isoquinoline 화합물과 1종의 quinolone 화합물을 분리하였다. Isoquinoline 화합물은 thalifoline (2)과 칼슘 방출을 저해하여 혈압조절에 관여하는 berberine (3)으로 구조동정 되었다. 또한 quinolone 화합물은 N-methylatanine (1)로 구조동정 되었다. 이들의 화합물 중에서 N-methylatanine (1)과 thalifoline (2)는 황백나무에서 처음으로 분리하였다.

• Parasites, Hosts and Diseases
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• 제51권6호
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• pp.711-717
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• 2013

Opisthorchis viverrini (O. viverrini) is a well-known causative agent of cholangiocarcinoma (CCA) in humans. CCA is very resistant to chemotherapy and is frequently fatal. To understand the pathogenesis of CCA in humans, a rodent model was developed. However, the development of CCA in rodents is time-consuming and the xenograft-transplantation model of human CCA in immunodeficient mice is costly. Therefore, the establishment of an in vivo screening model for O. viverrini-associated CCA treatment was of interest. We developed a hamster CCA cell line, Ham-1, derived from the CCA tissue of O. viverrini-infected and N-nitrosodimethylamine-treated Syrian golden hamsters. Ham-1 has been maintained in Dulbecco's Modified Essential Medium supplemented with 10% fetal bovine serum for more than 30 subcultures. These cells are mostly diploid (2n=44) with some being polyploid. Tumorigenic properties of Ham-1 were demonstrated by allograft transplantation in hamsters. The transplanted tissues were highly proliferative and exhibited a glandular-like structure retaining a bile duct marker, cytokeratin 19. The usefulness of this for in vivo model was demonstrated by berberine treatment, a traditional medicine that is active against various cancers. Growth inhibitory effects of berberine, mainly by an induction of G1 cell cycle arrest, were observed in vitro and in vivo. In summary, we developed the allo-transplantable hamster CCA cell line, which can be used for chemotherapeutic drug testing in vitro and in vivo.

• 한국식품과학회지
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• 제35권3호
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• pp.503-509
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• 2003

비만 저해 물질을 탐색하고자 전통의학에서 사용되는 200여 종류의 식용 또는 약용식물을 대상으로 지방세포 분화저해활성을 보이는 생약식물을 선별하여 용매층으로 활성이 이행되는 생약 중 황백(Phellodendri Cortex)을 최종적으로 후보식물로 선정하였다. 황백을 메탄올로 추출한 후, silica gel column chromatography, ODS RP-18 column chromatography, HPLC 등을 수행하여 지방세포 분화 저해활성을 갖는 화합물 PC-4를 분리하였다. PC-4는 노란색의 분말형태로 메탄올을 용매로 UV 최대흡수치를 조사한 결과, 222, 265, 349, 429 nm에서 최대의 UV의 흡수치를 보였으며 산이나 알카리 조건에서 최대 흡수치의 변화가 관찰되지 않았다. EI-mass spectrum을 측정한 결과, 분자량이 336.1로 예상되었으며 $^1H-NMR,\;^{13}C-NMR,\;DEPT,\;^1H-^1H\;COSY$, HMQC, HMBC NMR spectrum을 통해, PC-4는 isoquionoline alkaloide계열의 berberine으로 동정되었다. PC-4는 지방의 대사에 관여하는 중요한 효소인 fatty acid synthase와 pancreatic lipase에 대한 저해활성은 나타나지 않았으나, 지방세포 분화 저해활성은 $1\;{\mu}g/mL$의 낮은 농도에서도 관찰되었다.

• 대한임상검사과학회지
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• 제51권2호
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• pp.214-220
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• 2019

황련해독탕은 한방산업에서 염증관련 질환에 다빈도로 사용되는 처방이다. 본 연구의 목적은 전통적 한약인 황련해독탕의 항균활성을 확인하고 지표성분을 선정하는 것이다. 황련해독탕의 항균활성을 측정하기 위하여 디스크 확산법과 최소억제농도법을 사용하였다. 사용균주는 Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 23724였으며 대조군으로 oxacillin과 ciprofloxacin을 이용하여 황련해독탕의 항균활성을 확인하였다. 결과적으로 디스크 확산법에서는 S.aureus ATCC 25923에서 황련해독탕 20 mg과 10 mg은 $11.7{\pm}1.3mm$, $8.7{\pm}0.7mm$의 억제환을 형성하였으며 E.coli ATCC 23724에서는 황련해독탕 20 mg이 $8.0{\pm}0.0mm$의 억제환을 형성하였다. 최소억제농도는 S.aureus ATCC 25923가 31.25 mg/mL, E.coli ATCC 23724 에서는 125.0 mg/mL였다. 표준화를 위한 HPLC 정량분석 결과, 황련해독탕은 berberine을 16.55 mg/g 함유하고 있었으며 geniposide를 81.85 mg/g 함유하고 있었다. 천연물을 이용하여 항생제를 개발할 경우에는 내성세균을 억제하고 부작용을 줄일 수 있다는 장점이 있다. 이러한 점을 착안하여 지속적으로 연구를 진행한다면 한방산업과 더불어 여러 산업에서의 발전을 기대할 수 있을 것이다.

• 한국임상수의학회지
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• 제14권2호
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• pp.168-171
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• 1997

The present experiment was conducted to compare the therapeutic effects of laser irradiation on Kyo-So Hyul with those of antidisrrheal drugs gentamycin, berberine and sulfisomidine for 173 piglets with diarrhea as major symptoms. The results obtained were as follows; antidiarrheal drugs were effective in fifty nine (78%) of seventy six piglets and ineffective in seventeen (22%) of seventy six piglets. Laser irradiation on the Kyo-So Hyul were proved as effective in eighty eight (91%) of ninety seven piglets and invalid in nine (9%) of ninety seven piglets.

• Archives of Pharmacal Research
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• 제19권1호
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• pp.36-40
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• 1996

The effect of glycyrrhetinic acid(18.betha.-glycyrrhetinic acid, GA) on histamine synthesis and release was investigated in cocultured mast cells with Swiss 3T3 fibroblasts. GA has strong dose dependent inhibitory activity for histamine synthesis and release in cocultured mast cells. GA(50 .mu.M) inhibited about 85% of histidine decarboxylase (HDC) activity. The appearance of cells staining positively with berberine sulface was also decreased in the presence of GA. It indicates that transdifferentiation of cultured mas cells (CMCs) was also inhibited.

• 한국환경농학회지
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• 제34권2호
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• pp.128-133
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• 2015

BACKGROUND: Plant extracts have been used as environment friendly agricultural materials for organic farming in South Korea. However safety evaluation on the plant extracts was not properly tested. The aim of this study was to evaluate safety of the extracts from Melia azedarach, Nerium indicum and Coptis chinensis on cultivating rice. METHODS AND RESULTS: Pant extarcts 300-fold diluted were treated on rice, and residues of M. azedarach, N. indicum and C. chinensis were determined. The analytes from the rice samples were detected by liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS). The method was validated, and good linearities ($r^2=0.995-0.998$), specificity, and recoveries were obtained. Limits of detection were 0.01 mg/kg for all of the target compounds. Recoveries were 79.3-118.3% at 0.1 mg/kg and 75.2-111.5% at 0.5 mg/kg. The residue levels were below 0.030 mg/kg for azadirachtin, 0.320 mg/kg for oleandrin and 1.460 mg/kg for berberine. CONCLUSION(S): The extracts of M. azedarach, N. indicum and C. chinensis contained azadirachtin, oleandrin and berberine as an active ingredient, respectively. The residue of three active ingredients dramatically decreased after treatment in all fruits, stems and roots of rice.

• 한국의류학회지
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• 제25권3호
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• pp.617-628
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• 2001

Cotton and silk fabrics dyed with brazilein(Caesalpinia Sappan), berberine (Phellodendron Amurense), and shikonin(Lithospermum Erythrorhizon) were stored in the air, in the water, and under the ground for about one year. The change of the color, the flexibility, and the breaking strength were measured at the intervals of few weeks. The results are as follows; 1. The color of the fabrics changed most extensively in the fabrics that were under the ground, then in the order of the ones that were in the water, and in the air. The color of the fabrics changed to the achromatic color over the time. 2. The flexibility change among the dyes was similar. All the fabrics became stiff under the ground and in the water over the storage time. 3. In general, cotton and silk fabrics dyed with berberine showed better strength retention than the fabrics dyed with other substances. 4. The strength retention of cotton fabrics was high in the order of the fabrics which were in the air, in the water, and under the ground. Only minuscule change occurred in the strength of the cotton fabrics. The strength of the fabrics that were in the water and under the ground decreased remarkably after 30 weeks and 20 weeks respectively. 5. The strength retention of silk fabrics was high in the order of the fabrics which were in the air, under the ground, and in the water. In the air, the strength on silk fabrics decreased rapidly after 30 weeks. In the water, the strength of silk fabrics decreased more rapidly than that of the cotton fabrics. Under the ground, the strength retention of silk was higher than that of cotton.

• Journal of Pharmaceutical Investigation
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• 제35권4호
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• pp.249-254
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• 2005

Multidrug resistance (MDR) of cancer cells is, at least in part, associated with the overexpression of P-glycoprotein (P-gp). Many studies have demonstrated that natural compounds obtained from fruits, vegetables, teas and medicinal plants may modulate P-gp activity. The objective of the present investigation was to examine the effect of seven natural compounds on the P-gp activity in human uterine sarcoma cell line, MES-SA/DX5. Daunomycin uptake was significantly increased by biochanin A and silymarin (p<0.0001) whereas it was reduced by morin (p<0.01). The efflux of daunomycin from the cells was significantly inhibited by biochanin A, morin, cephalotaxine, berberine (p<0.05) and silymarin (p<0.0001). Biochanin A, berberine and silymarin significantly decreased $IC_{50}$ value of daunomycin (p<0.05) while morin increased it (p<0.05). These results suggest that some natural compounds such as biochanin A and silymarin may inhibit P-gp function and can be developed as MDR reversing agents to improve the efficacy of chemotherapeutic drugs when administered concomitantly.

• The Korean Journal of Physiology and Pharmacology
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• 제16권2호
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• pp.79-89
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• 2012

We examine whether Phellodendron amurense (PA) and its major alkaloid compound, berberine (BER), improved memory defects caused by administering scopolamine in rats. Effects of PA and BER on the acetylcholinergic system and pro-inflammatory cytokines in the hippocampus were also investigated. Male rats were administered daily doses for 14 days of PA (100 and 200 mg/kg, i.p.) and BER (20 mg/kg, i.p.) 30 min before scopolamine injection (2 mg/kg, i.p.). Daily administration of PA and BER improved memory impairment as measured by the passive avoidance test and reduced the escape latency for finding the platform in the Morris water maze test. Administration of PA and BER significantly alleviated memory-associated decreases in cholinergic immunoreactivity and restored brain-derived neurotrophic factor and cAMP-response element-binding protein mRNA expression in the hippocampus. PA and BER also decreased significantly the expression of proinflammatory cytokines such as interleukin-$1{\beta}$, tumor necrosis factor-${\alpha}$ and cyclooxygenase-2 mRNA in the hippocampus. These results demonstrated that PA and BER had significant neuroprotective effects against neuronal impairment and memory dysfunction caused by scopolamine in rats. These results suggest that PA and BER may be useful as therapeutic agents for improving cognitive functioning by stimulating cholinergic enzyme activity and alleviating inflammatory responses.