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http://dx.doi.org/10.4333/KPS.2005.35.4.249

Effect of Natural Compounds on P-glycoprotein Activity in Human Uterine Sarcoma Cells  

Chung, Soo-Yeon (College of Pharmacy, Ewha Womans University)
Go, Eun-Jung (College of Pharmacy, Ewha Womans University)
Kim, Na-Hyung (College of Pharmacy, Ewha Womans University)
Sung, Min-Kyung (College of Pharmacy, Ewha Womans University)
Jang, Jung-Ok (College of Pharmacy, Ewha Womans University)
Lee, Hwa-Jeong (College of Pharmacy, Ewha Womans University)
Publication Information
Journal of Pharmaceutical Investigation / v.35, no.4, 2005 , pp. 249-254 More about this Journal
Abstract
Multidrug resistance (MDR) of cancer cells is, at least in part, associated with the overexpression of P-glycoprotein (P-gp). Many studies have demonstrated that natural compounds obtained from fruits, vegetables, teas and medicinal plants may modulate P-gp activity. The objective of the present investigation was to examine the effect of seven natural compounds on the P-gp activity in human uterine sarcoma cell line, MES-SA/DX5. Daunomycin uptake was significantly increased by biochanin A and silymarin (p<0.0001) whereas it was reduced by morin (p<0.01). The efflux of daunomycin from the cells was significantly inhibited by biochanin A, morin, cephalotaxine, berberine (p<0.05) and silymarin (p<0.0001). Biochanin A, berberine and silymarin significantly decreased $IC_{50}$ value of daunomycin (p<0.05) while morin increased it (p<0.05). These results suggest that some natural compounds such as biochanin A and silymarin may inhibit P-gp function and can be developed as MDR reversing agents to improve the efficacy of chemotherapeutic drugs when administered concomitantly.
Keywords
P-glycoprotein; Natural compounds; Daunomycin; MES-SA/DX5 cells; Multidrug resistance;
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