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Adipocyte Differentiation Inhibitor Isolated from the Barks of Phellodendron amurense  

Kim, Kyung-Hee (Korea Research Institute of Bioscience and Biotechnology (KRIBB))
Ahn, Soon-Cheol (Korea Research Institute of Bioscience and Biotechnology (KRIBB))
Lee, Myung-Sun (Korea Research Institute of Bioscience and Biotechnology (KRIBB))
Kweon, Oh-Song (Korea Research Institute of Bioscience and Biotechnology (KRIBB))
Oh, Won-Keun (Korea Research Institute of Bioscience and Biotechnology (KRIBB))
Kim, Min-Soo (Korea Research Institute of Bioscience and Biotechnology (KRIBB))
Sohn, Cheon-Bae (Department of Food and Nutrition, Chungnam National University)
Ahn, Jong-Seog (Korea Research Institute of Bioscience and Biotechnology (KRIBB))
Publication Information
Korean Journal of Food Science and Technology / v.35, no.3, 2003 , pp. 503-509 More about this Journal
Abstract
For the development of the anti-obesity natural drug, the inhibitor of adipocyte differentiation was screened from Korean traditional medicinal plants. Phellodendri Cortex was selected as a candidate of adipocyte differentiation inhibitor. An inhibitory compound PC-4 was purified from the methanol (MeOH) extract of Phellodendri Cortex using silica gel and ODS RP-18 column chromatography and HPLC. PC-4 was obtained as yellow powder; UV ${\lambda}_{max}$ (MeOH): 230, 260, 340 and 430 nm. The chemical structure of PC-4 was determined as an isoquionoline alkaloid, berberine, on the basis of various NMR experiments including $^1H-\;and\;^{13}C-NMR$. The PC-4 inhibited the differentiation of preadipocyte NIH-3T3 L1 cells at a concentration of $1\;{\mu}g/mL$.
Keywords
Phellodendri Cortex; inhibitor; adipocyte differentiation; Phellodendron amurense; berberine;
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1 Michell, A.L. Becoming fat. Genes Develop. 16: 1-5 (2002)   DOI   PUBMED   ScienceOn
2 Kim, J.P., Jung, M.Y., Kim, J.P. and Kim, S.Y. Antiphotooxidative activity of protoberberines derived from Coptis japonica Makino in the chlorophyll-sensitized photooxidation of oil. J. Agric. Food Chem. 48: 1058-1063 (2000)   DOI   ScienceOn
3 Iwasa, K., Moriyasu, M., Yamori, T., Turuo, T., Lee, D.U. and Wolfgang, W. In vitro cytotoxicity of the protoberberine-type alkaloids. J. Nat. Prod. 64: 896-898 (2001)   DOI   ScienceOn
4 Park, K.S., Kang, K.C., Kim, J.H., Adams, D.J., lohng, T.N. and Paik, Y.K. Differentiation inhibitory effects of protoberberines on sterol chitin biosyntheses in Candida albicans. British J. Antimicrob. Chemother. 43: 667-674 (1999)   DOI   ScienceOn
5 Sabir, M. and Bhide, N.K. Study of some pharmacological actions of berberine. Indian J. Physiol. Pharmacol. 15: 111-132 (1971)
6 Kim, Y.S. Transcriptional factors involved in insulin resistance. Korean J. Lipidol. 9: 28-35 (1999)
7 Mitani, N., Murakami, K., Yamaura, T., Ikeda, T. and Saiki, I. Inhibitory effect of berberine on the mediastinal lymph node metastasis produced by orthotopic implantation of Lewis lung carcinoma. Cancer Lett. 165: 35-42 (2001)   DOI   ScienceOn
8 Huang, K.C. The Pharmacology of Chinese Herbs, p. 289. CRC press, Tokyo, Japan (1993)
9 Kim, J.B. New horizon in atherosclerosis research: Insights into fat cell differentiation and insulin sensitivity with ADDl/SREBPl and PPAR$\gamma$. Korean J. Lipidol. 11: 79-83 (2001)
10 Loftus, T.M. and Lane, M.D. Modulating the transcriptional control of adipogenesis. Genetics Develop. 7: 603-606 (1997)
11 Robert, M.C., Robert, E.L. and Robert, E.M. Jr. Molecular regulation of adipocyte differentiation. Cell Develop. BioI. 10: 3-10 (1999)   DOI   ScienceOn
12 Michio, S., Moritake, H., Zhou, Y.T., Wang, M.Y., Christopher, B.N. and Roger, H.U. Lipoapoptosis in beta-cells of obese prediabetic fa/fa rats. J. BioI. Chem. 273: 32487-32490 (1998)   DOI   ScienceOn
13 Bradford, B.L. and Bruce, M.S. Towards a molecular understanding of adaptive thermogenesis. Nature 404: 652-660 (2000)   PUBMED
14 Jiang, M.S and Lane, M.D. Sequential repression and activation of the CCAAT enchancer-binding protein-$\alpha$ (C/EBP$\alpha$) gene during adipogensis. Proc. Natl. Acad. Sci. USA 97: 12519-12523 (2000)   DOI   ScienceOn
15 Szeto, S., Yow, C.M.N. and Fung, K.W. Characterization of berberine on human cancer cells in culture. Turk. J. Med. Sci. 32: 363-368 (2002)
16 Jeon, Y.W., Jung, J.W.., Kang, M.R., Chung, I.K. and Lee, W.T NMR studies on antitumor drug candidates, berberine and berberrubine. Bull. Korean Chem. Soc. 23: 391-394 (2002)   DOI
17 Holst, D. and Grimaldi, P.A. New factors in the regulation of adipose differentiation and metabolism. Lipidology 13: 241-245 (2002)
18 Ren$e, K., Gert, S. and Hesselink, M.K.C. Optimization of Oil red O staining permits combination with immunofluorescence and automated quantification of lipids. Histochem. Cell. Biol. 116: 63-68 (2001)   PUBMED
19 Uchiyama, T., Kamikawa, H. and Ogita, Z. Anti-ulcer effect of extract from Phellodendri cortex. Yakugaku Zasshi 109: 672-676 (1989)   PUBMED
20 Evan, D.R., Christopher, J.W., Pere, P. and Bruce, M.S. Transcriptional regulation of adipogenesis. Genes Develop. 14: 1293-1307 (2000)
21 Rosen, E.D., Hsu, C.H., Wang, X., Sakai, S., Freeman, M.W., Gonzalez, F.J. and Spiegelman, B.M. C/EBP$\alpha$ induces adipogenesis through PPAR$\gamma$: a unified pathway. Genes Develop. 16: 22-26 (2002)   DOI   ScienceOn
22 Dils, R. and Carey, E. M. Methods in Enzymol. 35: 74-83 (1975)   DOI   ScienceOn
23 Pan, J.F., Yu, C., Zhu, D.Y., Zhang, H., Zeng, J.F., Jiang, S.H. and Ren, J.Y. Identification of three sulfate-conjugated metabolites of berberine chloride in healthy volunteers' urine after oral administration. Acta Pharmacol. Sin. 23: 77-82 (2002)   PUBMED
24 Tang, Q.Q. and Lane, M.D. Role of C/EBP homologous protein (CHOP-10) in the programmed activation of CCAAT/enhancerbinding protein-$\beta$ during adipogensis. Proc. Natl. Acad. Sci. USA 97: 12446-12450 (2000)   DOI   ScienceOn
25 Andrea, B., Laura, G. and Alexander, S. Rapamycin inhibits human adipocyte differentiation in primary culture. Obesity Res. 8: 249-254 (2000)
26 Ramirez-Zacarias, J.L., Castro-Muiiozledo, F. and Kuri-Harcuch, W. Quantiation of adipose conversion and triglycerides by staining intracytoplasmic lipids with Oil red O. Histochemistry 493-497 (1992)
27 Tracy, C.L. Purification and crystallization of rat liver fatty acid synthetase. Arch. Biochem. Biophys. 209: 613-619 (1981)   DOI   PUBMED   ScienceOn
28 Xueming, G. and Kan, L. Analysis of gene expression profile during 3T3-L1 preadipocyte differentiation. Gene 251: 45-53 (2000)   DOI   ScienceOn
29 Spiegelman, B.A. and Flier, J.S. Adipogenesis and obesity: Rounding out the big picture. Cell 87: 377-389 (1996)   DOI   ScienceOn
30 Chen, D. and Garg A. Monogenic disorders of obesity and body fat distribution. J. Lipid Res. 40: 1735-1746 (1999)   PUBMED