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Material Characteristics and Provenance Interpretation for Chloritic Beads from the Boseong Geoseokri and Haenam Buntori Sites, Korea (보성 거석리 및 해남 분토리 유적 출토 녹니석제 구슬의 재질특성과 원산지 해석)

  • Kim, Ji-Young;Lee, Chan-Hee;Kim, Jin-Young
    • Journal of Conservation Science
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    • v.23
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    • pp.25-37
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    • 2008
  • This study focuses on identification of mineralogical and geochemical characteristics and interpretation of raw material sources for prehistoric chlorite beads excavated from Geoseokri site in Boseong and Buntori site in Haenam, Korea. These prehistoric beads consist of three grayish blue ring-shaped beads, one dark green tubular bead and one greenish black tubular bead that show acicular-columnar and fibrous microtexture. The beads are composed of $SiO_2$, $Al_2O_3$, MgO and FeO as majors and a trace amount of $K_2O$, CaO and Na_2O$. Mineral species is mostly chlorite with a small amount of quartz and feldspar. Quantitative analysis indicates that the grayish blue ring-shaped beads and the dark green tubular bead belong to clinochlore and the greenish black tubular bead does to the boundary between clinochlore and sheridantie. Chlorite is a hydrous phyllosilicate mineral and it shows various microtexture of acicular, sheeted, earthy, granular andfibrous shapes. As its hardness is 2, chlorite is easily engraved due to its softness. It has aesthetic worthy as it shows green, black and greenish gray colors and pearly to greasy luster as well. These factors would lead to the extensive use of chloritic beads as ornaments from prehistoric times. Though the mineral sources of the chlorite beads can be found in central western region of Chungnam and Iwon of Hamnam, those areas are too distant from the two relic sites. Instead, chlorite ores commonly occur as altered products in wall rock alteration zone of every hydrothermal deposit. Therefore, it is probable that raw materials of chlorite were supplied from neighboring hydrothermal environment rather than far deposits. The result needs further study to verify raw material provenance interpretation, supply, manufacture and distribution on the basis of archaeological points of view.

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Occurrence of Papaya ringspot virus Infecting Cucurbit Crops in Korea (박과작물에 발생하는 파파야원형반점바이러스의 발생 보고)

  • Jin, Tae-Seong;Kim, Sang-Mok;Ko, Sug-Ju;Lee, Su-Heon;Choi, Hong-Soo;Park, Jin-Woo;Cha, Byeong-Jin
    • The Korean Journal of Pesticide Science
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    • v.13 no.4
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    • pp.298-308
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    • 2009
  • A flexuous rod-shaped virus was isolated from Cucurbita pepo leaves showing as green mosaic and puckering symptoms at Anseong, Korea. Based on the biological analysis, electron microscopy, and reverse transcription-polymerase chain reaction (RT-PCR), the virus isolate was identified as Papaya ringspot virus type watermelon (PRSV-W). From biological analysis, the host range of PRSV-W was limited to the families Cucurbitaceae and Chenopodiaceae. Most susceptible cucurbit species, such as Cucumis lanatus, Cucumis sativus, Cucurbita pepo, and Citrullus lanatus, showed symptoms of green mosaic, malformation, puckering, and narrow laminae by infection with PRSV-W. The local lesion were showed on the inoculated leaves of both Chenopodium amaranticolor and C. quinoa. Field survey of PRSV, Watermelon mosaic virus (WMV) and Zucchini yellow mosaic virus (ZYMV), three major viruses infecting cucurbit, was done during 2001 to 2003 on 173 commercial cucurbit cultivating fields distributed over the three regions of Gyeonggi, Gyeongbuk and Jeonnam Provinces where cucurbits are grown in different environmental conditions and cropping patterns. Typical viral symptoms were observed from 107 cultivating fields, and all three kinds of potyviruses were detected from 206 samples out of the 235 samples using RT-PCR. Watermelon mosaic virus (WMV) and Zucchini yellow mosaic virus (ZYMV) are the most widely distributed viruses in outdoor and retarding-culture fields, at an infection rating of 48 and 33 percents, respectively. PRSV was detected from 12 percent of 235 samples. The nucleotide and amino acid sequences of coat proteins (CP) of eight PRSV isolates, collected from several areas including Anseong, were determined and sequenced heterogeneity among the isolates was performed. The CP gene of PRSV showed 88.6~97.3 percent homology in nucleotide sequences and 95.1~99.3 percent homology in amino acid sequences with other PRSV isolates worldwide. The phylogenetic analysis indicated that the Korean PRSV isolates belong to the southern-east Asian cluster.

Study on the Biodegradability of Dispersants and Dispersant/Bunker-C Oil Mixtures and the Dissolved Oxygen Consumption in the Seawater(II) - The Biodegradability of Dispersant/Bunker-C Oil Mixtures and the Dissolved Oxygen Consumption in the Seawater - (해수중에서 유처리제 및 유처리제/Bunker-C유 혼합물의 생분해도와 용존산소소비에 관한 연구(II) - 유처리제/Bunker-C유 혼합물의 생분해도와 용존산소소비 -)

  • KIM Gwang-Su;PARK Chung-Kil;KIM Jong-Gu
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.26 no.6
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    • pp.519-528
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    • 1993
  • The biodegradation experiment, the TOD analysis and the element analysis for dispersant, Bunker-C and dispersant/Bunker-C oil mixtures were conducted for the purposes of evaluating the biodegradability of dispersnat/Bunker-C oil mixtures and studying the consumption of dissolved oxygen with relation to biodegradation in the seawater. The results of biodegradation experiment showed the mixtures with $1:10{\sim}5:10$ mix ratios of dispersant to 4mg/l of Bunker-C oil to be $0.34{\sim}2.06mg/l$ of $BOD_5$ and to be $1.05{\sim}5.47mg/l$ of $BOD_{20}$ in natural seawater. The results of TOD analysis showed 1mg of Bunker-C oil to be 3.16mg of TOD. The results of element analysis showed the contents of carbon and hydrogen to be $87.3\%\;and\;11.5\%$ for Bunker-C oil, respectively, but nitrogen element was not detected in Bunker-C oil. The biodegradability of dispersant/Bunker-C oil mixture shown as the ratio of $BOD_5$/TOD was increased from $3\%\;to\;11\%$ as a mix ratio of dispersant to 4mg/l of Bunker-C oil changed from 1:10 to 5:10, and the mixtures were found to belong in the organic matter group of low-biodegradability. The deoxygenation rates($K_1$) and ultimate oxygen demands($L_o$) obtained through the biodegration experiment and Thomas slope method were found to be $0.072{\sim}0.097/day$ and $1.113{\sim}6.746mg/l$ for the mixtures with $1:10{\sim}5:10$ mix ratios of dispersant to 4mg/l of Bunker-C oil, respectively. The ultimate oxygen demand of mixture was increased as a mix ratio of dispersant to Bunker-C oil changed from 1:10 to 10:5. This means that the more dispersants are applied to the sea for Bunker-C oil cleanup, the more decreases the dissolved oxygen level in the seawater.

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Effects of Cover Plants on Soil Microbial Community in Organic Apple Orchards (피복작물이 유기 사과과원 토양미생물상에 미치는 영향)

  • Oh, Young-Ju;Kang, Seok-Boem;Song, Yang-Ik;Choi, Jin-Ho;Paik, Woen-Ki
    • Korean Journal of Soil Science and Fertilizer
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    • v.45 no.5
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    • pp.822-828
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    • 2012
  • Organic fruit production has increased due to consumer's interest and government's political support for environmentally-friendly agriculture. The aim of this study was to investigate the effects of cover plants on soil microbial community and establish the fruit cultivation method by organic farming techniques. Cover plants used as an organic nutrient source in an apple orchard were rye and barley, the Gramineae and red clover and hairy vetch, the Leguminosae. In the effects of cover plants on the soil chemical characteristics, the soil pH values were higher than that of conventional organic pear orchard. The content of P showed no significant difference between control and cover plant plots. Organic matter level was similar in control and Gramineae cover plant plots, while organic matter content in cover plants belong to Leguminosae was lower than that of control plot. K content was lower in the plots treated with rye and red clover than control plot, while K content in hairy vetch treated plot was higher than control plot. Ca content was lower in control plot than in cover plant treated plots. Concentrations of Mg in the plots treated with barley and hairy vetch was lower than control plot. In August rye and red clover covered soil showed higher bacterial community density than that of control soil and barley treated soil showed highest Actinomycetes community density among treatments. Barley and hairy vetch soils showed higher level of fungi community density than that of control soil in August. In pyrosequencing analysis barley treated soil showed highest distribution ratio of Actinomycetes among treatment. Our findings might be used as basic data for choosing cover plant with effective organic matter decomposition and nutrition supply capacity.

Assessment of the Value and Distribution of Geological Heritages in Korea: Jeolla Province (한국의 지질유산 분포와 가치평가: 전라권)

  • Cho, Hyeongseong;Kang, Hee-Cheol;Kim, Jong-Sun;Cheong, Daekyo;Paik, In Sung;Lim, Hyoun Soo;Choi, Taejin;Kim, Hyun Joo;Roh, Yul;Cho, Kyu-Seong;Huh, Min;Shin, Seungwon
    • The Journal of the Petrological Society of Korea
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    • v.28 no.4
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    • pp.319-345
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    • 2019
  • Recently planification and effort for management, assessment and discovery of geological heritagesare being increasingly demanded with institutional strategies such as geopark, as their preservation is asked socially. In this study, we discovered geological heritages in the Jeolla Province and then performed assessment of the value and grading of them and finally suggested a promising and suitable site for the National Geopark. A total of 325 geological heritages are listed on literature review and then detailed description in field and assessment of the value for selected 158 geoheritages are completed. The assessment items are categorized into intrinsic value, subsidiary value, and preservation/management part. The intrinsic value is subdivided into scientific/educational value, composed of representativeness, rarity, geodiversity, typicality, reproducibility, and particularity, and geomorphological/landscape value composed of scale, naturality (integrity), scenery (aesthetic value). Also, subsidiary value consist of 7 subsections of soil function, ecological function, tourism value, geological resource, historical value, folk tale or legend and symbolic value, and accessibility, convenient facility (infrastructure), management condition (legal protection) is evaluated in preservation/management part. The heritages in the Jeolla Province subdivided into three types: 73 geological heritages, 42 geomorphological heritages, and 42 composite heritages. Based on points acquired in intrinsic value, all geological heritages are graded Class-I to -V. As a result, numbers of geoheritage belong to Class-I (protection at world level), -II (protection at national level), -III (nationdesignated management), -IV (involved management list), -V (candidate management list) are 12, 39, 52, 34, 21, respectively. Finally, we construct database based on Arc-GIS with all informations for each geological heritage and suggest three promising and suitable sites, 'Jirisan-Seomjingang Area' and 'south coast area of Jeolla Province', for the National Geopark.

Geographic Variations and DNA Polymorphisms in Gizzard-shad (Konosirus punctatus) (전어 (Konosirus punctatus)의 지리적 변이와 DNA 다형성)

  • Park, Su-Young;Kim, Jong-Yeon;Yoon, Jong-Man
    • Korean Journal of Ichthyology
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    • v.18 no.4
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    • pp.300-310
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    • 2006
  • Genomic DNA isolated from three geographical gizzard-shad (Konosirus punctatus) populations in Seocheon (SC), Busan (BS) and Gochang (GC) collected in the West Sea and the southern sea, respectively, off the Korean Peninsula, were PCR-amplified repeatedly. Eight selected decamer and 20-mer primers generated a total of 713 loci in the SC population, 791 in the BS population, and 732 in the GC population, with a DNA fragment size ranging from 100 bp to 2,800 bp. We identified 50 unique loci for the SC population, 70 unique loci for the BS population and 130 for the GC population: 120 shared loci for the three populations. There were 108 specific loci (15.1%) for the SC population, 74 (9.4%) for the BS population, and 67 (9.2%) for the GC population. Eight primers also generated 48 polymorphic loci (6.7%) for the SC population, 26 (3.3%) for the BS population, and 16 (2.2%) for the GC population. The similarity matrix ranged from 0.756 to 0.936 for the SC population, from 0.800 to 0.938 for the BS population, and from 0.731 to 0.959 for the GC population. The dendrogram obtained by the eight primers indicates three genetic clusters: cluster 1 (SEOCHEON 01~SEOCHEON 10), cluster 2 (BUSAN 11~BUSAN 20 and GOCHANG 23~GOCHANG 24), and cluster 3 (GOCHANG 21, 22, 25, 26, 27, 28, 29 and 30). As stated above, some individuals of the GC population appear to belong in BS population. When seeing this result, it was thought with the fact that some individuals of 2 populations seem to come and go partially. Thus, RAPD-PCR analysis revealed a significant genetic distance between the three geographical gizzard-shad populations. Using various decamer and 20-mer primers, RAPD-PCR may be applied to identify specific/polymorphic markers that are particular to a species and geographic population, and to define genetic diversity, polymorphisms, and similarities among geographical gizzard-shad populations.

What Do Scientists Think about the Nature of Science? - Exploring Views of the Nature of Science of Korean Scientists Related with Life Science Area (우리나라 생명과학 관련 분야 재미 과학자들은 어떻게 과학의 본성을 이해하고 있는가?)

  • Lee, Young Hee
    • Journal of The Korean Association For Science Education
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    • v.34 no.7
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    • pp.677-691
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    • 2014
  • Understanding of the nature of science (NOS) has been a consistent topic as one of the most important goals in science education for the past several decades. Even though there is a variety of research related with the NOS conducted in science education, few researches has been conducted for the conception of scientists regards to the nature of science (Bayir et al., 2014; Taylor et al., 2008; Wong & Hudson, 2008). Recently, researchers in science education turned their attention to identifying views of scientists about the nature of science since they recognized the importance of participation of scientists in science education (Southerland et al., 2003; Taylor et al., 2008). This study was conducted to examine the Korean scientists' views of the nature of science. Through the use of semi-structured questionnaire and in-depth interview the views of 35 scientists who belong to the Korean-American Scientists and Engineers Association (KSEA) regards to the nature of science were explored. Findings show that while the scientists have more informed views with respect to the tentativeness of scientific knowledge, cultural and social influence embedded in science, the limitation of science, and the collaboration of science with others, the scientists have more na${\ddot{i}}$ve views about the distinction between laws and theories, the existence of a universal scientific method, and the importance of imagination and creativity. As such, it can be assumed that the scientists cannot conceptualize their notion in a philosophical sense even though they are engaged in scientific work in reality (Bayir et al., 2014).

Genetic Divergence and Phylogenetic Relationships among the Korean Fireflies, Hotaria papariensis, Luciola lateratis, and Pyrocoelia rufa(Coleoptera: Lampyridae), using Mitochondrial DNA Sequences (미토콘드리아 DNA의 염기서열을 이용한 파파리반딧불이, 애반딧불이 및 늦반딧불이 (딱정벌레목: 반딧불이과)의 유전적 분화 및 계통적 관련)

  • 김익수;이상철;배진식;진병래;김삼은;김종길;윤형주;양성렬;임수호
    • Korean journal of applied entomology
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    • v.39 no.4
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    • pp.211-226
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    • 2000
  • Genetic divergence and phylogenetic relationships among the major Korean fireflies (Hotaria papariensis, Luciola lateralis, and Pyrocoelia rufa) were studied. A portion of mitochondrial COI (403 bp) and 165 rRNA (490~504 bp) genes were sequenced, and the GenBank-registered, homologous 165 rRNA sequences of Japanese fireflies were compared (27 species of Lampyridae, one of Lycidae, and one of Rhgophthalmidae). Greatest DNA and/or amino acid sequence divergence was found when P rufa, belonging to Lampyrinae was compared with H. papariensis and L. lateralis, both belong-ing to Luciolinae, confirming the current taxonomic status of the species. In the PAUP and PHYLIP analyses with 165 rRNA data, grouping of the two geographic samples of H. papariensis with H. tsushimana validate the use of generic name, Hotaria. Nevertheless, lack of sister-group relationship of the two geographic samples of H. papariensis renders further investigation on this group . Although the Korean and Japanese L. lateralis formed a strong monophyletic group, a substantial genetic differentiation was detected between them (2.9% of 165 rRNA gene sequence divergence). Finally, the geographic samples of Korean p. rufa strongly formed a group with Japanese p. rufa, warranting the use of generic name, Pyrocoelia, but the genetic distance observed between the Cheju-Island individual and all others requires further investigation on this subject. Summarized, this study supports the current taxonomic status of the Korean fireflies in that each respectively formed a strong monophyletic group with its own species or genus.

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Binding Mode Analysis of Bacillus subtilis Obg with Ribosomal Protein L13 through Computational Docking Study

  • Lee, Yu-No;Bang, Woo-Young;Kim, Song-Mi;Lazar, Prettina;Bahk, Jeong-Dong;Lee, Keun-Woo
    • Interdisciplinary Bio Central
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    • v.1 no.1
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    • pp.3.1-3.6
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    • 2009
  • Introduction: GTPases known as translation factor play a vital role as ribosomal subunit assembly chaperone. The bacterial Obg proteins ($Spo{\underline{0B}}$-associated ${\underline{G}}TP$-binding protein) belong to the subfamily of P-loop GTPase proteins and now it is considered as one of the new target for antibacterial drug. The majority of bacterial Obgs have been commonly found to be associated with ribosome, implying that these proteins may play a fundamental role in ribosome assembly or maturation. In addition, one of the experimental evidences suggested that Bacillus subtilis Obg (BsObg) protein binds to the L13 ribosomal protein (BsL13) which is known to be one of the early assembly proteins of the 50S ribosomal subunit in Escherichia coli. In order to investigate binding mode between the BsObg and the BsL13, protein-protein docking simulation was carried out after generating 3D structure of the BsL13 structure using homology modeling method. Materials and Methods: Homology model structure of BsL13 was generated using the EcL13 crystal structure as a template. Protein-protein docking of BsObg protein with ribosomal protein BsL13 was performed by DOT, a macro-molecular docking software, in order to predict a reasonable binding mode. The solvated energy minimization calculation of the docked conformation was carried out to refine the structure. Results and Discussion: The possible binding conformation of BsL13 along with activated Obg fold in BsObg was predicted by computational docking study. The final structure is obtained from the solvated energy minimization. From the analysis, three important H-bond interactions between the Obg fold and the L13 were detected: Obg:Tyr27-L13:Glu32, Obg:Asn76-L13:Glu139, and Obg:Ala136-L13:Glu142. The interaction between the BsObg and BsL13 structures were also analyzed by electrostatic potential calculations to examine the interface surfaces. From the results, the key residues for hydrogen bonding and hydrophobic interaction between the two proteins were predicted. Conclusion and Prospects: In this study, we have focused on the binding mode of the BsObg protein with the ribosomal BsL13 protein. The interaction between the activated Obg and target protein was investigated with protein-protein docking calculations. The binding pattern can be further used as a base for structure-based drug design to find a novel antibacterial drug.

Effect of Market Basket Size on the Accuracy of Association Rule Measures (장바구니 크기가 연관규칙 척도의 정확성에 미치는 영향)

  • Kim, Nam-Gyu
    • Asia pacific journal of information systems
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    • v.18 no.2
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    • pp.95-114
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    • 2008
  • Recent interests in data mining result from the expansion of the amount of business data and the growing business needs for extracting valuable knowledge from the data and then utilizing it for decision making process. In particular, recent advances in association rule mining techniques enable us to acquire knowledge concerning sales patterns among individual items from the voluminous transactional data. Certainly, one of the major purposes of association rule mining is to utilize acquired knowledge in providing marketing strategies such as cross-selling, sales promotion, and shelf-space allocation. In spite of the potential applicability of association rule mining, unfortunately, it is not often the case that the marketing mix acquired from data mining leads to the realized profit. The main difficulty of mining-based profit realization can be found in the fact that tremendous numbers of patterns are discovered by the association rule mining. Due to the many patterns, data mining experts should perform additional mining of the results of initial mining in order to extract only actionable and profitable knowledge, which exhausts much time and costs. In the literature, a number of interestingness measures have been devised for estimating discovered patterns. Most of the measures can be directly calculated from what is known as a contingency table, which summarizes the sales frequencies of exclusive items or itemsets. A contingency table can provide brief insights into the relationship between two or more itemsets of concern. However, it is important to note that some useful information concerning sales transactions may be lost when a contingency table is constructed. For instance, information regarding the size of each market basket(i.e., the number of items in each transaction) cannot be described in a contingency table. It is natural that a larger basket has a tendency to consist of more sales patterns. Therefore, if two itemsets are sold together in a very large basket, it can be expected that the basket contains two or more patterns and that the two itemsets belong to mutually different patterns. Therefore, we should classify frequent itemset into two categories, inter-pattern co-occurrence and intra-pattern co-occurrence, and investigate the effect of the market basket size on the two categories. This notion implies that any interestingness measures for association rules should consider not only the total frequency of target itemsets but also the size of each basket. There have been many attempts on analyzing various interestingness measures in the literature. Most of them have conducted qualitative comparison among various measures. The studies proposed desirable properties of interestingness measures and then surveyed how many properties are obeyed by each measure. However, relatively few attentions have been made on evaluating how well the patterns discovered by each measure are regarded to be valuable in the real world. In this paper, attempts are made to propose two notions regarding association rule measures. First, a quantitative criterion for estimating accuracy of association rule measures is presented. According to this criterion, a measure can be considered to be accurate if it assigns high scores to meaningful patterns that actually exist and low scores to arbitrary patterns that co-occur by coincidence. Next, complementary measures are presented to improve the accuracy of traditional association rule measures. By adopting the factor of market basket size, the devised measures attempt to discriminate the co-occurrence of itemsets in a small basket from another co-occurrence in a large basket. Intensive computer simulations under various workloads were performed in order to analyze the accuracy of various interestingness measures including traditional measures and the proposed measures.